STRAIN: SE8R5

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breseq  version 0.22  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSE8R5_1_sequence.txt4,672,899471,962,799100.0%101.0 bases101 bases96.8%
errorsSE8R5_2_sequence.txt4,672,598471,932,398100.0%101.0 bases101 bases95.9%
total9,345,497943,895,197100.0%101.0 bases101 bases96.4%

Reference Sequence Information

seq idlengthfit meanfit dispersiondescription
coveragedistributionNC_0140343,738,958239.92.7Rhodobacter capsulatus SB 1003 chromosome, complete genome.
coveragedistributionNC_014035132,962137.31.8Rhodobacter capsulatus SB 1003 plasmid pRCB133, complete sequence.
total3,871,920

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

Junction Evidence

reference sequencepr(no read start)
NC_0140340.35518
NC_0140350.53920

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Polymorphism E-value cutoff2
Polymorphism frequency cutoffOFF
Polymorphism minimum coverage each strandOFF
Polymorphism homopolymer length cutoffOFF
Polymorphism bias cutoff0
Polymorphism predict indel polymorphismsOFF

Execution Times

stepstartendelapsed
Read and reference sequence file input08:55:54 22 Mar 201308:59:50 22 Mar 20133 minutes 56 seconds
Read alignment to reference genome08:59:51 22 Mar 201309:41:42 22 Mar 201341 minutes 51 seconds
Preprocessing alignments for candidate junction identification09:41:42 22 Mar 201309:49:37 22 Mar 20137 minutes 55 seconds
Preliminary analysis of coverage distribution09:49:37 22 Mar 201310:00:10 22 Mar 201310 minutes 33 seconds
Identifying junction candidates10:00:10 22 Mar 201310:00:19 22 Mar 20139 seconds
Re-alignment to junction candidates10:00:19 22 Mar 201310:13:57 22 Mar 201313 minutes 38 seconds
Resolving alignments with junction candidates10:13:57 22 Mar 201310:26:41 22 Mar 201312 minutes 44 seconds
Creating BAM files10:26:41 22 Mar 201310:36:10 22 Mar 20139 minutes 29 seconds
Tabulating error counts10:36:10 22 Mar 201310:39:33 22 Mar 20133 minutes 23 seconds
Re-calibrating base error rates10:39:33 22 Mar 201310:39:37 22 Mar 20134 seconds
Examining read alignment evidence10:39:37 22 Mar 201311:16:04 22 Mar 201336 minutes 27 seconds
Polymorphism statistics11:16:04 22 Mar 201311:16:17 22 Mar 201313 seconds
Output11:16:17 22 Mar 201311:33:36 22 Mar 201317 minutes 19 seconds
Total 2 hours 37 minutes 41 seconds