Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,840 Δ1 bp 2.1% intergenic (+27/+130) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8400C→. 2.1% 4.2 ‑1.2 ‑0.1186intergenic (+27/+130)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (68/114):  total (68/118)
Fisher's exact test strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.73e-01

Only 100 of 203 total aligned reads displayed.

GGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG  >  NC_014034/1303742‑1303919
                                                                                                      |                                                                               
ggcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGGTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGc                                                                                 >  1:3813917/1‑101 (MQ=255)
 gcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGcc                                                                                >  1:681014/1‑101 (MQ=255)
  cgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCt                                                                               <  2:2986430/101‑1 (MQ=255)
  cgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCt                                                                               >  2:1083553/1‑101 (MQ=255)
   gcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTc                                                                              <  2:2719788/101‑1 (MQ=255)
   gcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTc                                                                              >  1:295109/1‑101 (MQ=255)
     ggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAc                                                                            >  2:157184/1‑101 (MQ=255)
     ggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAc                                                                            <  1:770578/101‑1 (MQ=255)
       tGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCccc                                                                          >  1:2676906/1‑101 (MQ=255)
         gCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAccccc                                                                        <  1:1026270/101‑1 (MQ=39)
          cGATGGAGGGCACGAAGGACAGCCTCTCCCCCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGCCGCTG..CCCCCTCGGCTTCGCCTCACCCCCg                                                                       <  1:3971448/101‑1 (MQ=255)
           gATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTTACCCCCgg                                                                      >  1:1754909/1‑101 (MQ=255)
           gATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCgg                                                                      >  2:1868296/1‑101 (MQ=39)
           gATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAACCCCgg                                                                      >  2:4439935/1‑101 (MQ=255)
              ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGat                                                                   <  2:360240/101‑1 (MQ=38)
              ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGat                                                                   <  2:3680532/101‑1 (MQ=38)
              ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCGG..CCCCCTCGGCTTCGCCTCACCCCCGGGat                                                                   >  1:2060014/1‑101 (MQ=255)
               gAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGata                                                                  <  2:2541706/101‑1 (MQ=38)
               gAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGata                                                                  <  1:391459/101‑1 (MQ=38)
                aGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGatat                                                                 <  1:238094/101‑1 (MQ=38)
                 gggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATAtt                                                                <  2:3870730/101‑1 (MQ=38)
                 gggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATAtt                                                                <  1:29160/101‑1 (MQ=38)
                  ggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATAttt                                                               <  2:2071938/101‑1 (MQ=38)
                  ggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATAttt                                                               <  1:118296/101‑1 (MQ=38)
                    cACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgg                                                             <  2:1809427/101‑1 (MQ=38)
                     aCGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTggg                                                            <  1:3415657/101‑1 (MQ=38)
                      cGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgggg                                                           <  2:70247/101‑1 (MQ=38)
                      cGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgggg                                                           >  1:603463/1‑101 (MQ=38)
                      cGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgggg                                                           <  1:1447642/101‑1 (MQ=38)
                      cGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGCTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgggg                                                           <  1:3739743/101‑1 (MQ=37)
                       gAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCCCCCCGGGATATTTGGGGc                                                          >  2:3730921/1‑101 (MQ=38)
                       gAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGc                                                          <  1:3376921/101‑1 (MQ=38)
                       gAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGc                                                          >  2:1199525/1‑101 (MQ=38)
                        aaGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTGCCCCCC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCa                                                         >  2:1483551/1‑101 (MQ=38)
                        aaGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCa                                                         >  2:2672922/1‑101 (MQ=38)
                        aaGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCa                                                         <  2:3757404/101‑1 (MQ=38)
                        aaGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCa                                                         <  1:2522488/101‑1 (MQ=38)
                         aGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCaa                                                        <  1:2766694/101‑1 (MQ=37)
                         aGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCaa                                                        >  2:4066991/1‑101 (MQ=37)
                          ggACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAg                                                       <  1:222855/101‑1 (MQ=37)
                           gACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGa                                                      <  1:1868420/101‑1 (MQ=37)
                           gACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGa                                                      >  2:2567570/1‑101 (MQ=37)
                             cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATa                                                    <  1:1058716/101‑1 (MQ=37)
                             cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATa                                                    <  1:3025391/101‑1 (MQ=37)
                             cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATa                                                    <  2:775306/101‑1 (MQ=37)
                             cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATa                                                    <  1:3727151/101‑1 (MQ=37)
                              aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGGGGTGCCCCCC..CGGCTTCGCCTCCCCCCCCGGGTATTTGGGGGAAGATaa                                                   >  1:1552880/1‑101 (MQ=255)
                              aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATaa                                                   >  2:1963024/1‑101 (MQ=37)
                              aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATaa                                                   <  1:3569241/101‑1 (MQ=37)
                              aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATaa                                                   >  1:3646637/1‑101 (MQ=37)
                               gCCTGTCCCGCGAACCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGATG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATaaa                                                  <  2:3396055/101‑1 (MQ=25)
                               gCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATaaa                                                  <  1:4429985/101‑1 (MQ=37)
                                 cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAAcc                                                <  1:909789/101‑1 (MQ=37)
                                 cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAAcc                                                <  2:497128/101‑1 (MQ=37)
                                  tGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAAccc                                               >  1:2817650/1‑101 (MQ=37)
                                    tCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCa                                             <  1:1356658/101‑1 (MQ=37)
                                     cccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCAt                                            <  1:981910/101‑1 (MQ=37)
                                         cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCtt                                        >  2:1236502/1‑101 (MQ=37)
                                         cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCtt                                        <  2:3924512/101‑1 (MQ=37)
                                         cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCGCACCCCCGGGATATTTGGGGCAAGATAAAACCCATCCtt                                        >  2:2516216/1‑101 (MQ=25)
                                          gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCttt                                       >  2:954734/1‑101 (MQ=37)
                                           aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTc                                      <  2:1655431/101‑1 (MQ=37)
                                           aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTc                                      <  1:4183610/101‑1 (MQ=37)
                                           aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTc                                      >  1:1964968/1‑101 (MQ=37)
                                             aCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCAc                                    <  1:4067251/101‑1 (MQ=37)
                                               ccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCt                                  <  2:1309633/101‑1 (MQ=37)
                                                cTGCGCGCCGTGGTCAGCGTTTAGGCGAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCtt                                 <  1:2694111/101‑1 (MQ=25)
                                                cTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCtt                                 <  2:1157380/101‑1 (MQ=37)
                                                 tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTg                                <  1:2340026/101‑1 (MQ=37)
                                                   cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGcc                              >  2:268817/1‑101 (MQ=37)
                                                   cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGcc                              <  2:709830/101‑1 (MQ=37)
                                                     cgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGc                            <  1:2484888/101‑1 (MQ=37)
                                                        cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCaaa                         <  1:3252659/101‑1 (MQ=37)
                                                         gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAat                        <  1:548970/101‑1 (MQ=37)
                                                         gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAat                        >  1:945980/1‑101 (MQ=25)
                                                         gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGAAAat                        <  2:603395/101‑1 (MQ=25)
                                                           ggTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCTCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAatat                      <  1:4408920/101‑1 (MQ=37)
                                                            gTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATc                     <  1:4091011/101‑1 (MQ=37)
                                                             tCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATcc                    <  2:3109187/101‑1 (MQ=37)
                                                               aGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCg                  <  2:2251175/101‑1 (MQ=37)
                                                                 cGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCggg                >  2:3450970/1‑101 (MQ=25)
                                                                 cGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCggg                <  2:1935375/101‑1 (MQ=37)
                                                                   tttAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCggggg              <  2:2141317/101‑1 (MQ=37)
                                                                      aGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCCCCTTGCCGCAAATATCCCGGGGGGTg           <  2:2817443/101‑1 (MQ=25)
                                                                      aGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg           >  2:3034117/1‑101 (MQ=25)
                                                                       ggCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa          <  2:433091/101‑1 (MQ=37)
                                                                        gCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa         >  2:627859/1‑101 (MQ=25)
                                                                        gCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa         <  1:2713358/101‑1 (MQ=37)
                                                                        gCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa         <  2:2298036/101‑1 (MQ=37)
                                                                        gCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGaa         >  1:3093844/1‑101 (MQ=25)
                                                                         cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGGCAGATAAACCCCATACTTTCACCTTGCCGCAAATATTCCGGGGGGGGact        >  2:4417341/1‑99 (MQ=21)
                                                                         cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt        <  1:2281954/101‑1 (MQ=37)
                                                                          ctgggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt       <  1:2093029/99‑1 (MQ=25)
                                                                          aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt       <  2:537103/101‑1 (MQ=37)
                                                                          aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt       <  1:3714195/101‑1 (MQ=37)
                                                                           aGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg      <  2:2710675/101‑1 (MQ=37)
                                                                           aGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg      <  2:3443337/101‑1 (MQ=37)
                                                                            gggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAAGATCCCGGGGGGTGAAtgag     >  2:2092892/1‑98 (MQ=25)
                                                                            gggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg     <  1:3824735/101‑1 (MQ=25)
                                                                               ggCGCTG..CCCCCGCGGCTTCGCGACACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTGGCCGCAAATGTCCCGGGGGGTGAATTGgccg  <  2:4177995/101‑1 (MQ=25)
                                                                                                      |                                                                               
GGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGATAAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG  >  NC_014034/1303742‑1303919

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG