Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,464 Δ1 bp 4.6% intergenic (+67/+81) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4640G→. 4.6% 17.5 ‑1.2 ‑0.3172intergenic (+67/+81)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (71/93):  total (76/96)
Fisher's exact test strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.59e-01

Only 100 of 186 total aligned reads displayed.

CGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGC  >  NC_014034/432365‑432562
                                                                                                      |                                                                                                   
cgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAg                                                                                                     <  1:4073935/101‑1 (MQ=255)
 gcACGCTTTCGGCGGCGGCGGGCCACGCCGGAGGCCGGGCAGGGGGGCGCGGCCCCCCGGC.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGc                                                                                                    <  1:2678140/101‑1 (MQ=21)
  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                                                   <  2:686013/101‑1 (MQ=255)
  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCCGGATATTTTAGGCAAGATGAAAGcc                                                                                                   >  2:1331927/1‑101 (MQ=21)
   aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                                                  <  1:3722350/101‑1 (MQ=255)
       tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCTCCGGGGTATTTGAGGCAAGATGAAGCCCGATcc                                                                                              >  1:3850092/1‑101 (MQ=21)
        ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                             <  1:1968169/101‑1 (MQ=255)
            gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                         <  2:941398/101‑1 (MQ=255)
             cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                        <  1:2731068/101‑1 (MQ=255)
              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                       <  2:2773287/101‑1 (MQ=255)
              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                       >  2:2595712/1‑101 (MQ=255)
               gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                      <  2:1134490/101‑1 (MQ=255)
                 ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                    <  1:2047272/101‑1 (MQ=255)
                  gggggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                   <  1:3136909/99‑1 (MQ=255)
                   cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGGTTc                                                                                  >  2:4038378/1‑101 (MQ=255)
                    gggCCACGCCTGAGGCCGGGCAGCGGGGCGCTGCCCCCC.GTCC.TGCGAACTCCCCACGGGATATTTGAGGCAAGATGAGAGCAGATCCGGGCTTCGTTGCa                                                                                 >  2:736359/1‑101 (MQ=18)
                      gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                               <  2:2054554/101‑1 (MQ=255)
                       ccACGCCTGAGGCCGGGGAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                              <  1:1267872/101‑1 (MQ=255)
                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                             <  2:173876/101‑1 (MQ=255)
                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                             >  2:3922964/1‑101 (MQ=255)
                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                             <  2:1778673/101‑1 (MQ=255)
                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCCTCGTTTCATcct                                                                             >  2:797111/1‑99 (MQ=255)
                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCAGATCCGGGCTTCGTTTTACCtt                                                                             >  2:59682/1‑101 (MQ=18)
                         aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                                                            <  1:1877662/101‑1 (MQ=255)
                          cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                           <  2:3170758/101‑1 (MQ=255)
                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                          >  2:3974588/1‑101 (MQ=255)
                             cTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGGTATTTGAGGTAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                        >  1:3680842/1‑101 (MQ=21)
                             cTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                        <  1:2609731/101‑1 (MQ=255)
                             cTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                        <  1:719163/101‑1 (MQ=255)
                              tGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                       <  1:599427/101‑1 (MQ=255)
                                  gCCGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                   <  2:2481959/101‑1 (MQ=255)
                                    cGGGCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                                                                 <  2:3175109/101‑1 (MQ=255)
                                      ggacggggggCTCTGCCCCCC.GG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAtt                                                   >  1:1895554/5‑101 (MQ=2)
                                       gacggggggCTCTGCCCCCC.GG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttg                                                  >  1:3903003/4‑100 (MQ=2)
                                       gCAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                              <  1:1391283/101‑1 (MQ=255)
                                        cAGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTAGTTTCATCTTGCCCCAAATATCCCgg                                                             >  1:3553983/1‑101 (MQ=25)
                                        acggggggCTCTGCCCCCC.GG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttgg                                                 >  2:3465243/3‑99 (MQ=2)
                                         cggggggCTCTGCCCCCC.GG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttggc                                                <  1:2878129/100‑4 (MQ=2)
                                         aGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                            >  1:2153747/1‑101 (MQ=255)
                                         aGGGGGGCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                            >  2:1951916/1‑101 (MQ=255)
                                          ggggggCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                           <  2:458048/101‑1 (MQ=255)
                                            ggggCGCTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGCTATTTGAGGCAATACGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCTAAATATCCCGGGGGt                                                         >  2:3000739/1‑101 (MQ=25)
                                              ggCGCTGCCCCCC.GT.CTTGCGGCCGCCCCCCGGCATATTTGAGGCAGGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.a                                                      <  2:4257360/101‑1 (MQ=25)
                                               gcccTGCCCCCC.GTCC.TGCGGGCCTCCCCCGGGATAATTGAGGGAAAATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.aa                                                     <  2:2566367/98‑1 (MQ=25)
                                                cgcTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAt                                                    >  2:127379/1‑101 (MQ=255)
                                                cgcTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAt                                                    >  1:337882/1‑101 (MQ=255)
                                                  cTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTTCCCCAAATATCCCCGGGGGG.AAttt                                                  >  2:36233/1‑101 (MQ=25)
                                                  cTGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAttt                                                  <  2:307592/101‑1 (MQ=255)
                                                   tGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCTTCTTGCCCCAAATATCCCGGGGGGG.AAtttt                                                 >  1:4268833/1‑101 (MQ=25)
                                                   tGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                 >  2:1915128/1‑101 (MQ=255)
                                                   tGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                 >  1:2809354/1‑101 (MQ=255)
                                                   tGCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCAGGACTTCGTTTCATCTTGCCCCAAATATCCCCGGGGTG.AAtttt                                                 >  2:4412183/1‑101 (MQ=25)
                                                   cgCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                 <  1:2284194/100‑1 (MQ=255)
                                                    gCCCCCC.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTg                                                >  2:3455892/1‑101 (MQ=255)
                                                      ccccc.GTCC.TGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTTATCTTGCCCCCAATATTCCGGGGGGGAAATTTTgg                                               >  2:3130228/1‑101 (MQ=21)
                                                       cccc.GTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGgcg                                             <  1:1370521/101‑1 (MQ=255)
                                                        ccc.GTCC.TGCGGACTCCCCCCGGGCTATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGgcgc                                            >  2:3342972/1‑101 (MQ=255)
                                                          ccgtcc.TGCGGACTCCCCCCGGGATATTTTAGGCAAAATGAAAACCGAACCGGGGTTCGTTTCATCTTGCCCCCAAAATCCCCGGGGGG.AATTTTgtggcc                                           >  2:1539672/6‑96 (MQ=18)
                                                          ccgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCa                                           <  2:667298/96‑1 (MQ=255)
                                                           cgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAg                                          <  1:3467986/97‑1 (MQ=255)
                                                           cgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATACGGGCCTCGTTTCATTTTGCCCCAAATATCCCGGGGGGG.GATTTTGGAGCAg                                          >  1:3947264/5‑101 (MQ=18)
                                                             tcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGcc                                        <  1:4379286/99‑1 (MQ=255)
                                                             tcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGcc                                        <  1:4015698/99‑1 (MQ=255)
                                                              cc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCa                                       >  1:3461589/2‑101 (MQ=255)
                                                                   cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAag                                   <  1:2162705/101‑1 (MQ=255)
                                                                    ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAaga                                  <  1:2020531/101‑1 (MQ=255)
                                                                        tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGTCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                              <  2:3680589/101‑1 (MQ=255)
                                                                        tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                              >  1:747322/1‑101 (MQ=255)
                                                                        tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                              >  2:4361890/1‑101 (MQ=255)
                                                                        tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                              >  2:2668008/1‑101 (MQ=255)
                                                                        gccccccGGAATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                              <  2:2826603/100‑1 (MQ=255)
                                                                          cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGc                            >  1:951457/1‑101 (MQ=255)
                                                                           ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCa                           <  1:4364882/101‑1 (MQ=255)
                                                                            cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCat                          >  1:1123477/1‑100 (MQ=255)
                                                                             ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgc                         <  1:478951/101‑1 (MQ=255)
                                                                              cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                        <  1:3640770/101‑1 (MQ=255)
                                                                              cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                        >  2:1422484/1‑101 (MQ=255)
                                                                              cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAccg                        <  2:2949293/101‑1 (MQ=255)
                                                                               gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                       >  2:1119944/1‑101 (MQ=255)
                                                                                 gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                     >  2:2771542/1‑101 (MQ=255)
                                                                                   tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                   <  2:3763654/101‑1 (MQ=255)
                                                                                    atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                  >  1:1211740/1‑101 (MQ=255)
                                                                                    atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                  >  2:201369/1‑101 (MQ=255)
                                                                                    atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                  <  1:1560879/101‑1 (MQ=255)
                                                                                    atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                  >  1:3175898/1‑101 (MQ=255)
                                                                                    atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                  >  2:4278323/1‑101 (MQ=255)
                                                                                     tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                 >  2:1853137/1‑101 (MQ=255)
                                                                                     tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                 >  1:2401888/1‑101 (MQ=255)
                                                                                      ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                <  2:1386528/101‑1 (MQ=255)
                                                                                      ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                <  1:3309251/101‑1 (MQ=255)
                                                                                       tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc               >  1:3586026/1‑101 (MQ=255)
                                                                                       tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCCATc               >  1:2845410/1‑101 (MQ=255)
                                                                                         aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc             >  1:3760642/1‑101 (MQ=255)
                                                                                           gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg           <  2:2077529/101‑1 (MQ=255)
                                                                                            cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc          >  2:1616172/1‑101 (MQ=255)
                                                                                              aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg        >  1:150635/1‑101 (MQ=255)
                                                                                               gATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc       <  1:769328/101‑1 (MQ=255)
                                                                                                 tGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc     >  2:1599185/1‑101 (MQ=255)
                                                                                                  gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg    >  1:1472402/1‑101 (MQ=255)
                                                                                                    aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc  <  1:1050148/101‑1 (MQ=255)
                                                                                                      |                                                                                                   
CGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGC  >  NC_014034/432365‑432562

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG