Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,471 Δ1 bp 4.8% intergenic (+74/+74) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4710C→. 4.8% 17.7 ‑0.7 ‑0.1167intergenic (+74/+74)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (73/84):  total (80/87)
Fisher's exact test strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.86e-01

Only 100 of 184 total aligned reads displayed.

TTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCG  >  NC_014034/432372‑432571
                                                                                                    |                                                                                                     
tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCTCCGGGGTATTTGAGGCAAGATGAAGCCCGATcc                                                                                                       >  1:3850092/1‑101 (MQ=21)
    ggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGc                                                                                                   >  1:2776396/1‑101 (MQ=255)
     gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                                  <  2:941398/101‑1 (MQ=255)
      cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                                 <  2:2928521/101‑1 (MQ=255)
       ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                                <  1:1214384/101‑1 (MQ=255)
       ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                                >  2:2595712/1‑101 (MQ=255)
          ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                             <  1:2047272/101‑1 (MQ=255)
           gggggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                            <  1:3136909/99‑1 (MQ=255)
            cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGGTTc                                                                                           >  2:4038378/1‑101 (MQ=255)
              ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                         >  2:3436232/1‑101 (MQ=255)
               gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                        <  2:2054554/101‑1 (MQ=255)
                 cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                      <  2:173876/101‑1 (MQ=255)
                 cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                      >  2:3922964/1‑101 (MQ=255)
                 cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                      <  2:1778673/101‑1 (MQ=255)
                 cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCAGATCCGGGCTTCGTTTTACCtt                                                                                      >  2:59682/1‑101 (MQ=18)
                  aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                                                                     <  1:1877662/101‑1 (MQ=255)
                  aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                                                                     <  1:1916388/101‑1 (MQ=255)
                   cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                                    >  1:558702/1‑101 (MQ=255)
                   cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                                    <  2:3170758/101‑1 (MQ=255)
                   cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                                    <  1:2370166/101‑1 (MQ=255)
                    gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                                   >  2:3974588/1‑101 (MQ=255)
                      cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTATTTGAGGTAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                 >  1:3680842/1‑101 (MQ=21)
                      cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                 <  1:2609731/101‑1 (MQ=255)
                      cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                                 <  1:719163/101‑1 (MQ=255)
                       tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                                <  1:599427/101‑1 (MQ=255)
                          ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                             <  2:2979145/101‑1 (MQ=255)
                            ccGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                           <  2:2311039/101‑1 (MQ=255)
                              gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                                                                         <  1:2715247/101‑1 (MQ=255)
                               ggacggggtgCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAtt                                                            <  2:1564473/97‑1 (MQ=2)
                               ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAtt                                                            >  1:1895554/5‑101 (MQ=2)
                                gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttg                                                           >  1:3903003/4‑100 (MQ=2)
                                gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                       <  1:2000895/101‑1 (MQ=255)
                                 acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttgg                                                          >  2:3465243/3‑99 (MQ=2)
                                  cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttggc                                                         <  1:2878129/100‑4 (MQ=2)
                                  aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                     <  2:1915454/101‑1 (MQ=255)
                                  aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                     >  1:2153747/1‑101 (MQ=255)
                                   ggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttggcc                                                        <  1:1418386/101‑5 (MQ=2)
                                   ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                                    >  1:1315167/1‑101 (MQ=255)
                                    gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGCGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggggg                                                                   >  2:3663196/1‑101 (MQ=255)
                                     ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                                                                  <  2:1267855/101‑1 (MQ=255)
                                      gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                                 <  1:3436471/101‑1 (MQ=255)
                                      gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                                 <  2:593968/101‑1 (MQ=255)
                                       ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.a                                                               <  2:1963676/101‑1 (MQ=255)
                                        gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.aa                                                              <  1:4038635/101‑1 (MQ=255)
                                        gcccTGCCCCCCGTCCTGCGGGCCTCCCCCGGGATAATTGAGGGAAAATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.aa                                                              <  2:2566367/98‑1 (MQ=25)
                                           cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAttt                                                           <  2:307592/101‑1 (MQ=255)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCTTCTTGCCCCAAATATCCCGGGGGGG.AAtttt                                                          >  1:4268833/1‑101 (MQ=25)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                          >  2:1915128/1‑101 (MQ=255)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                          >  2:748745/1‑101 (MQ=255)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                          <  1:1490855/101‑1 (MQ=255)
                                            tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCAGGACTTCGTTTCATCTTGCCCCAAATATCCCCGGGGTG.AAtttt                                                          >  2:4412183/1‑101 (MQ=25)
                                            cgCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                          <  1:2284194/100‑1 (MQ=255)
                                             gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTg                                                         >  2:3067673/1‑101 (MQ=255)
                                               cccccGTCCTGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTTATCTTGCCCCCAATATTCCGGGGGGGAAATTTTgg                                                        >  2:3130228/1‑101 (MQ=21)
                                                  ccgtccTGCGGACTCCCCCCGGGATATTTTAGGCAAAATGAAAACCGAACCGGGGTTCGTTTCATCTTGCCCCCAAAATCCCCGGGGGG.AATTTTgtggcc                                                    >  2:1539672/6‑96 (MQ=18)
                                                  ccgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCa                                                    <  2:667298/96‑1 (MQ=255)
                                                   cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAg                                                   <  1:3467986/97‑1 (MQ=255)
                                                    gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGTCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGc                                                  >  2:4209462/4‑101 (MQ=255)
                                                     tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGcc                                                 <  1:4379286/99‑1 (MQ=255)
                                                       cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCaa                                               >  2:3699599/1‑101 (MQ=255)
                                                       cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCaa                                               <  2:723720/101‑1 (MQ=255)
                                                        tGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCaaa                                              >  1:1386625/1‑101 (MQ=255)
                                                         gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCaaaa                                             <  1:3923218/101‑1 (MQ=255)
                                                           ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAaga                                           <  1:2020531/101‑1 (MQ=255)
                                                           ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAaga                                           >  1:3575681/1‑101 (MQ=255)
                                                              cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAggg                                        <  2:3314560/101‑1 (MQ=255)
                                                               tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                       >  2:4361890/1‑101 (MQ=255)
                                                               tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                       >  2:2668008/1‑101 (MQ=255)
                                                               tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                       >  1:747322/1‑101 (MQ=255)
                                                                 cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGc                                     >  1:951457/1‑101 (MQ=255)
                                                                   cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCat                                   >  1:1123477/1‑100 (MQ=255)
                                                                   cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAg                                   <  2:2776206/101‑1 (MQ=255)
                                                                    ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                  <  1:478951/101‑1 (MQ=255)
                                                                    ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGG.AATTTTGGCGCAGCCAAAAGAGGGGACAgc                                  >  1:3726286/1‑101 (MQ=255)
                                                                     cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                 <  1:3640770/101‑1 (MQ=255)
                                                                     cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                 >  2:1422484/1‑101 (MQ=255)
                                                                      gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                                >  1:2225443/1‑101 (MQ=255)
                                                                      gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                                >  2:1119944/1‑101 (MQ=255)
                                                                        gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                              >  2:2771542/1‑101 (MQ=255)
                                                                           atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                           >  1:3175898/1‑101 (MQ=255)
                                                                           atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                           <  1:1560879/101‑1 (MQ=255)
                                                                            tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                          >  1:2401888/1‑101 (MQ=255)
                                                                            tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                          >  2:1853137/1‑101 (MQ=255)
                                                                             ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                         <  2:1386528/101‑1 (MQ=255)
                                                                             ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                         <  1:3309251/101‑1 (MQ=255)
                                                                              tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                        >  1:3586026/1‑101 (MQ=255)
                                                                                 ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                     >  2:1302902/1‑101 (MQ=255)
                                                                                 ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                     <  2:4268564/101‑1 (MQ=255)
                                                                                   cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc                   <  2:3461349/101‑1 (MQ=255)
                                                                                   cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc                   >  2:1616172/1‑101 (MQ=255)
                                                                                     aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                 <  1:3967083/101‑1 (MQ=255)
                                                                                      gATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc                <  1:769328/101‑1 (MQ=255)
                                                                                         gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg             >  1:1472402/1‑101 (MQ=255)
                                                                                           aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc           <  1:1050148/101‑1 (MQ=255)
                                                                                              ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc        <  2:1123079/101‑1 (MQ=255)
                                                                                              ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc        <  2:863435/101‑1 (MQ=255)
                                                                                                gATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc      >  1:2813568/1‑101 (MQ=255)
                                                                                                 aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgcg     <  1:3000960/101‑1 (MQ=255)
                                                                                                  tCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGcggcgcggcggcgcgg    >  2:2632513/1‑101 (MQ=255)
                                                                                                    cGGGCTTCGTTTCATCTTGCCGCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGgcgcggcggcgcggcg  <  2:4085539/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
TTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCG  >  NC_014034/432372‑432571

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG