Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,831 +T 96.9% intergenic (+18/+139) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8312.→T96.9% 9.7 0.0 ‑0.5195intergenic (+18/+139)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (74/115):  ref base (2/4):  total (76/119)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.34e-01

Only 100 of 223 total aligned reads displayed.

GCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG  >  NC_014034/1303732‑1303919
                                                                                                      |                                                                                           
 cTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ct                                                                                          <  2:3347905/101‑2 (MQ=255)
 cTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..cc                                                                                          >  2:3462454/1‑100 (MQ=255)
  tCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ctc                                                                                         >  1:433131/1‑99 (MQ=255)
   cAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGGGCTG..CCCc..ctcg                                                                                        >  1:471466/1‑98 (MQ=255)
   cAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ctcg                                                                                        <  1:212127/101‑4 (MQ=255)
   cAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ctcg                                                                                        >  1:2478284/1‑98 (MQ=255)
    aaaCGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ctcgg                                                                                       <  1:1711297/101‑5 (MQ=255)
      aCGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCc..ctcggct                                                                                     <  2:3740994/101‑7 (MQ=255)
           gcgcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGcc                                                                                  >  1:681014/1‑101 (MQ=255)
             gcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTc                                                                                <  2:185510/101‑1 (MQ=255)
             gcggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTc                                                                                <  2:2719788/101‑1 (MQ=255)
               ggTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAc                                                                              <  1:770578/101‑1 (MQ=255)
                gTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAcc                                                                             >  2:2705618/1‑101 (MQ=255)
                 tGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAccc                                                                            >  2:1170963/1‑101 (MQ=255)
                  ggCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAcccc                                                                           <  1:3724255/101‑1 (MQ=255)
                   gCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCAccccc                                                                          <  1:1026270/101‑1 (MQ=39)
                    cGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCcccccc                                                                         >  1:3757657/1‑100 (MQ=255)
                     gATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTTACCCCCgg                                                                        >  1:1754909/1‑101 (MQ=255)
                      aTGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCggg                                                                       <  2:518430/101‑1 (MQ=39)
                        ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGat                                                                     <  2:622757/101‑1 (MQ=38)
                        ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGat                                                                     <  2:360240/101‑1 (MQ=38)
                        ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGat                                                                     <  2:3680532/101‑1 (MQ=38)
                        ggAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCGG..CCCCCTCGGCTTCGCCTCACCCCCGGGat                                                                     >  1:2060014/1‑101 (MQ=255)
                         gAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGata                                                                    <  2:2541706/101‑1 (MQ=38)
                          aGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGatat                                                                   <  1:3949095/101‑1 (MQ=38)
                          aGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGatat                                                                   <  1:238094/101‑1 (MQ=38)
                          aGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGatat                                                                   >  2:1717776/1‑101 (MQ=37)
                           gggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATAtt                                                                  <  2:3870730/101‑1 (MQ=38)
                            ggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATAttt                                                                 <  1:118296/101‑1 (MQ=38)
                            ggCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATAttt                                                                 <  2:2071938/101‑1 (MQ=38)
                              cACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgg                                                               <  2:471414/101‑1 (MQ=38)
                               aCGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTggg                                                              <  1:3415657/101‑1 (MQ=38)
                                cGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgggg                                                             <  1:1447642/101‑1 (MQ=38)
                                cGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgggg                                                             >  1:603463/1‑101 (MQ=38)
                                cGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTgggg                                                             <  2:70247/101‑1 (MQ=38)
                                 gAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCCCCCCGGGATATTTGGGGc                                                            >  2:3730921/1‑101 (MQ=38)
                                 gAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGc                                                            <  1:3376921/101‑1 (MQ=38)
                                  aaGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTGCCCCCC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCa                                                           >  2:1483551/1‑101 (MQ=38)
                                  aaGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCa                                                           <  2:3757404/101‑1 (MQ=38)
                                   aGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCaa                                                          <  2:1895797/101‑1 (MQ=37)
                                    ggACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAg                                                         <  1:222855/101‑1 (MQ=37)
                                     gACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGa                                                        >  2:2567570/1‑101 (MQ=37)
                                     gACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGa                                                        <  1:1868420/101‑1 (MQ=37)
                                     gACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCCCCTCACCCCCGGGGTATTTGGGGCAAGa                                                        >  2:3042755/1‑101 (MQ=37)
                                      aCAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGGTATTTGGGGCAAGAt                                                       >  2:2677245/1‑101 (MQ=37)
                                       cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.a                                                     <  2:775306/101‑1 (MQ=37)
                                       cAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.a                                                     <  1:1058716/101‑1 (MQ=37)
                                        aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aa                                                    >  2:1963024/1‑101 (MQ=37)
                                        aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aa                                                    >  1:1935504/1‑101 (MQ=37)
                                        aGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aa                                                    <  1:3569241/101‑1 (MQ=37)
                                         gCCTGTCCCGCGAACCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGATG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.aaa                                                   <  2:3396055/101‑1 (MQ=25)
                                          ccTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc                                                  <  1:372764/101‑1 (MQ=37)
                                           cTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGGTATTTGGGGCAAGAT.AAAc.c                                                >  2:3936609/1‑101 (MQ=37)
                                            tGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc.cc                                               >  1:2817650/1‑101 (MQ=37)
                                             gTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAc.ccc                                              <  1:3090249/101‑1 (MQ=37)
                                              tCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCa                                             <  1:1356658/101‑1 (MQ=37)
                                                ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                           <  1:2504877/101‑1 (MQ=37)
                                                ccGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATc                                           <  1:297474/101‑1 (MQ=37)
                                                   cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCtt                                        >  2:1236502/1‑101 (MQ=37)
                                                   cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCGCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCCtt                                        >  2:2516216/1‑101 (MQ=25)
                                                    gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCttt                                       >  2:549019/1‑101 (MQ=37)
                                                    gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCttt                                       >  2:954734/1‑101 (MQ=37)
                                                     aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                      <  1:4183610/101‑1 (MQ=37)
                                                     aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                      <  2:1153367/101‑1 (MQ=37)
                                                     aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCATTTc                                      >  2:1634156/1‑101 (MQ=25)
                                                      aaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCa                                     <  1:2941568/101‑1 (MQ=37)
                                                        cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAcc                                   <  1:2909380/101‑1 (MQ=37)
                                                         ccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCt                                  <  2:1309633/101‑1 (MQ=37)
                                                         ccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCt                                  <  1:268955/101‑1 (MQ=37)
                                                           tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTg                                <  1:2340026/101‑1 (MQ=37)
                                                            gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGc                               >  1:4069879/1‑101 (MQ=37)
                                                            gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGc                               <  2:232175/101‑1 (MQ=37)
                                                             cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGcc                              <  2:709830/101‑1 (MQ=37)
                                                              gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCg                             >  1:381672/1‑101 (MQ=37)
                                                                  cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                         <  1:3252659/101‑1 (MQ=37)
                                                                  cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                         <  1:2567733/101‑1 (MQ=37)
                                                                   gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAat                        <  1:548970/101‑1 (MQ=37)
                                                                      gTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                     <  2:4366074/101‑1 (MQ=37)
                                                                      gTCAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                     <  2:2894150/101‑1 (MQ=37)
                                                                        cAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATccc                   <  2:1415316/101‑1 (MQ=37)
                                                                        cAGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCCCAAATATccc                   >  1:4031346/1‑101 (MQ=25)
                                                                         aGCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCg                  <  2:2251175/101‑1 (MQ=37)
                                                                          gCGTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCCCCCCCGGGGTATTTGGGGCAAGAT.AAAC.CCCCTCCTTTCACCTTGCCGCAAATATCCCgg                 >  2:4200577/1‑101 (MQ=25)
                                                                            gTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg               >  2:1044292/1‑101 (MQ=37)
                                                                            gTTTAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg               <  1:2713752/101‑1 (MQ=37)
                                                                             tttAGGCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg              <  2:2141317/101‑1 (MQ=37)
                                                                             tttAGGCAAGGGGGCGATG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg              >  1:4411521/1‑101 (MQ=25)
                                                                                cggacggggggCTCTGC.CCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAAt        <  1:3652129/95‑1 (MQ=2)
                                                                                 ggCAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa          <  1:94295/101‑1 (MQ=37)
                                                                                   cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGGCAGAT.AAAC.CCCATACTTTCACCTTGCCGCAAATATTCCGGGGGGGGact        >  2:4417341/1‑99 (MQ=21)
                                                                                   cAAGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt        <  1:2281954/101‑1 (MQ=37)
                                                                                   acggggggCTCTGC.CCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTgg     <  2:3002218/98‑1 (MQ=2)
                                                                                    ctgggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt       <  1:2093029/99‑1 (MQ=25)
                                                                                    aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCGCCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt       <  1:2705808/101‑1 (MQ=25)
                                                                                    aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAAtt       >  1:953618/1‑101 (MQ=25)
                                                                                    aaGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATACCTGTTCCTTGCCACAAATATGCCgggggggcaact       >  1:3837157/1‑95 (MQ=12)
                                                                                     aGGGGGCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg      <  2:2710675/101‑1 (MQ=37)
                                                                                      gggggCTCTGC.CCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca  <  2:4155742/101‑2 (MQ=2)
                                                                                      gggggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAAGATCCCGGGGGGTGAAtgag     >  2:2092892/1‑98 (MQ=25)
                                                                                        gggCGCTG..CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc   <  2:344965/101‑1 (MQ=37)
                                                                                                      |                                                                                           
GCTCAAACGCGGCGCGGTGGCGATGGAGGGCACGAAGGACAGCCTGTCCCGCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG  >  NC_014034/1303732‑1303919

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG