Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,044,155 +C 8.3% intergenic (+28/+149) typA → / ← potG1 GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,044,1550.→C 8.3% 43.3 ‑0.5 ‑0.0193intergenic (+28/+149)typA/potG1GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand):  new base (6/9):  ref base (84/93):  total (91/102)
Fisher's exact test strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 223 total aligned reads displayed.

GCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCTTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCG  >  NC_014034/2044055‑2044252
                                                                                                                                                                                                       
gCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCt                                                                                                   >  1:3616968/1‑101 (MQ=255)
 ccGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCtt                                                                                                  <  1:2294286/101‑1 (MQ=255)
   gAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCtttt                                                                                                <  2:1898542/101‑1 (MQ=255)
   gAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCtttt                                                                                                <  2:3075108/101‑1 (MQ=255)
    aaGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCttttt                                                                                               <  1:2035461/101‑1 (MQ=255)
     aGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAAGGGGGGGGGCTCCCCCCCttttt                                                                                               >  1:3740143/1‑101 (MQ=255)
       aTCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCtttttcat                                                                                            <  1:3480228/101‑4 (MQ=255)
       aTCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCtttttcat                                                                                            <  2:3292707/101‑4 (MQ=255)
        tCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCtttttcatt                                                                                           >  2:153053/1‑97 (MQ=255)
        tCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCtttttcatt                                                                                           <  1:3336469/101‑5 (MQ=255)
         cGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTc                                                                                         <  1:1440343/101‑1 (MQ=255)
          gTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCg                                                                                        >  1:43969/1‑101 (MQ=255)
           tGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGt                                                                                       <  1:946053/101‑1 (MQ=255)
            gCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTg                                                                                      <  1:2724757/101‑1 (MQ=255)
            gCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTg                                                                                      <  1:1819798/101‑1 (MQ=255)
             cgcgtGCGCCAGCGGCTTCTGGTCCCGCACGAGCGCAAACGCGACTCGCGCTCGGAATAAGTCGTCAAAA.GCGGGGCTCTGCCCCCC.TTTTTCATTCGTGc                                                                                     <  2:3675140/97‑1 (MQ=255)
             cGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGc                                                                                     <  2:3865031/101‑1 (MQ=255)
              gTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGcc                                                                                    >  2:501359/1‑101 (MQ=255)
               tCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGcag                                                                                   <  1:3216494/101‑3 (MQ=255)
                cTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGa                                                                                  >  2:918590/1‑101 (MQ=255)
                   cgcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATg                                                                               <  1:1622724/101‑1 (MQ=255)
                   cgcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATg                                                                               <  1:2752239/101‑1 (MQ=255)
                    gcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGa                                                                              <  2:322770/101‑1 (MQ=255)
                    gcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGa                                                                              >  1:130625/1‑101 (MQ=255)
                    gcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGa                                                                              >  2:2483994/1‑101 (MQ=255)
                    gcAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGa                                                                              <  2:2072569/101‑1 (MQ=255)
                     cAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaa                                                                             >  2:80501/1‑101 (MQ=255)
                      aaGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGaaa                                                                            <  1:1352773/101‑1 (MQ=255)
                       aGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAAt                                                                           <  1:3451798/101‑1 (MQ=255)
                        gCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAAtt                                                                          <  2:3337743/101‑1 (MQ=255)
                          ggCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCa                                                                        <  2:1606828/101‑1 (MQ=255)
                              tCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCCCCTc                                                                    >  2:3024335/1‑101 (MQ=255)
                               cTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTcc                                                                   >  1:3988667/1‑101 (MQ=255)
                                tGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTccc                                                                  >  1:2945481/1‑101 (MQ=255)
                                tGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTccc                                                                  <  2:3760192/101‑1 (MQ=255)
                                    cccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCg                                                              <  2:2900661/101‑1 (MQ=255)
                                     ccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCgg                                                             <  2:2543293/101‑1 (MQ=255)
                                     ccGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCgg                                                             >  1:1582638/1‑101 (MQ=255)
                                      cGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCggg                                                            <  1:1593787/101‑1 (MQ=255)
                                         aCGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGTCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGata                                                         >  2:57367/1‑101 (MQ=255)
                                         aCGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGata                                                         >  1:395024/1‑101 (MQ=255)
                                          cGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGatat                                                        <  2:4358869/101‑1 (MQ=255)
                                          cGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGatat                                                        <  2:3488112/101‑1 (MQ=255)
                                            aGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATAttt                                                      <  2:3616715/101‑1 (MQ=255)
                                             gcgcAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTg                                                     <  1:4318328/101‑1 (MQ=255)
                                                cAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAgg                                                  >  2:1264238/1‑101 (MQ=255)
                                                 aaaCGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGc                                                 >  1:2483077/1‑101 (MQ=255)
                                                 aaaCGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGc                                                 >  1:1191428/1‑101 (MQ=255)
                                                  aaCGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCCCCTCCCCCCGGGATATTTGAGGCa                                                <  1:4291574/101‑1 (MQ=255)
                                                     gcgcCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGa                                             >  1:1009519/1‑101 (MQ=255)
                                                       gcCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATa                                           <  1:2097430/101‑1 (MQ=255)
                                                         cTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGGTCTTCCCCCC.TTTTTCATTTGTGCCGAATGAAATTTAACTCCCCCCGGGGTATTTTAGGGAAGATaaa                                         >  2:1673635/1‑101 (MQ=255)
                                                         cTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATaaa                                         <  1:750048/101‑1 (MQ=255)
                                                         cTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATaaa                                         >  2:1871978/1‑101 (MQ=255)
                                                         cTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATaaa                                         <  2:1038762/101‑1 (MQ=255)
                                                           cgcgCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGa                                       >  1:462082/1‑101 (MQ=255)
                                                             cgcTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACt                                     <  2:3739890/101‑1 (MQ=255)
                                                              gcTTGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACtt                                    <  1:1683512/101‑1 (MQ=255)
                                                              gcTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACtt                                    <  2:3144349/101‑1 (MQ=255)
                                                              gcTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACtt                                    <  1:1386760/101‑1 (MQ=255)
                                                              gcTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACtt                                    <  2:4116936/101‑1 (MQ=255)
                                                               cTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTg                                   <  1:3389781/101‑1 (MQ=255)
                                                                 cGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCg                                 >  2:3942348/1‑101 (MQ=255)
                                                                 cGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCg                                 >  2:2387099/1‑101 (MQ=255)
                                                                  ggAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGa                                >  2:3556087/1‑101 (MQ=255)
                                                                   gAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAg                               >  1:4263083/1‑101 (MQ=255)
                                                                    aaTAAGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCCTTTTTGCCGAATGAAATTCACCTCCCCCCCGGATATTTTAGGGAAGATAAAGACTCGCGAgg                              >  2:888421/1‑101 (MQ=255)
                                                                       aaGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtgt                           >  1:1511387/1‑101 (MQ=255)
                                                                       aaGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtgt                           >  1:1407073/1‑101 (MQ=255)
                                                                       aaGACGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGgtgt                           >  1:1718016/1‑101 (MQ=255)
                                                                        aGACGTCAAAA.GGGGAGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGtt                          >  1:3752858/1‑101 (MQ=255)
                                                                          aCGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttct                        >  2:3147201/1‑101 (MQ=255)
                                                                           cGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttctt                       <  2:4054439/101‑1 (MQ=255)
                                                                           cGTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttctt                       >  1:565006/1‑101 (MQ=255)
                                                                            gTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttcttc                      >  2:672010/1‑101 (MQ=255)
                                                                            gTCAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGttcttc                      <  1:2079332/101‑1 (MQ=255)
                                                                              cAAAA.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCAt                    <  2:4036336/101‑1 (MQ=255)
                                                                               aaaa.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATc                   <  1:192078/101‑1 (MQ=255)
                                                                               aaaa.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGGGTTCTTCAtt                   >  1:3066018/1‑100 (MQ=255)
                                                                                aaa.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCt                  >  1:1027869/1‑101 (MQ=255)
                                                                                aaa.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGGGTTCTTCATCt                  >  1:1465424/1‑101 (MQ=255)
                                                                                 aa.GGGGGGCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCtt                 >  1:2374894/1‑101 (MQ=255)
                                                                                    ggggggCTCTGCCCCCCTTTTT..ATTTCTGCCGAATGAAAATCACCCCCCCCCGGGTTATTTTAGGGAAGAAAAAGACCTGCCGGGGGGTCTTCATCttttt              >  2:3994760/1‑99 (MQ=255)
                                                                                     gggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCt              >  2:1975505/1‑101 (MQ=255)
                                                                                      ggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTg             >  2:2356835/1‑101 (MQ=255)
                                                                                      ggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTg             <  2:1059713/101‑1 (MQ=255)
                                                                                      ggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTg             <  1:4185791/101‑1 (MQ=255)
                                                                                      ggggCTCTGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTg             >  2:1214547/1‑101 (MQ=255)
                                                                                          ctctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCaaa         <  1:3118993/101‑1 (MQ=255)
                                                                                            ctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGa       >  1:1996339/1‑101 (MQ=255)
                                                                                            ctGCCCCCC.TTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGa       <  2:1571318/101‑1 (MQ=255)
                                                                                             tGCCCCCCCTTTTT.TTTCGTGGCGAATGAAATTTAACTCCCCCCCGGGTATTTTAGGCAAGATAAAGACTTGTGCGGGGTTTTTCCTCTTTCTTCAAAGAc      >  2:284533/1‑101 (MQ=255)
                                                                                              tgcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAc      >  1:2194130/3‑101 (MQ=255)
                                                                                              tgcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAc      <  1:2525120/99‑1 (MQ=255)
                                                                                              tgcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAc      <  1:104693/99‑1 (MQ=255)
                                                                                               gcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAcc     >  2:3811209/2‑101 (MQ=255)
                                                                                               gcccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGAcc     <  2:1774429/100‑1 (MQ=255)
                                                                                                 ccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACcgc   >  1:1559940/1‑101 (MQ=255)
                                                                                                 ccccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACcgc   <  2:4330616/101‑1 (MQ=255)
                                                                                                  cccCTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACcgcg  >  1:3867903/1‑101 (MQ=255)
                                                                                                                                                                                                       
GCCGAAGATCGTGCGTCTGCGCAAGCGGCTTCTGGACCCGCACGAGCGCAAACGCGCCTCGCGCTCGGAATAAGACGTCAAAA.GGGGGGCTCTGCCCCCCTTTTTTCATTCGTGCCGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCG  >  NC_014034/2044055‑2044252

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 40 ≤  ATCG