Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,342 +G 100% coding (35/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3421.→G100.0% 641.9 172coding (35/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (85/86):  ref base (1/0):  total (86/86)

Only 100 of 201 total aligned reads displayed.

ACCTTCCTTCATCTTGACGAAAA..GGGTTG..G..TCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCC.A.G.GCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTG  >  NC_014034/2177248‑2177434
                                                                                                        |                                                                                               
aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.a                                                                                                  >  2:2366269/1‑101 (MQ=255)
aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.a                                                                                                  >  2:4198614/1‑101 (MQ=255)
 ccttccttCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g                                                                                                <  1:2640702/101‑1 (MQ=255)
 ccttccttCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g                                                                                                >  1:2687925/1‑101 (MQ=255)
 ccttccttCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g                                                                                                <  1:3327607/101‑1 (MQ=255)
 ccttccttCATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g                                                                                                <  1:1937434/101‑1 (MQ=255)
  cttccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGACGCCCCGGCCAGCGCGGAAAACGCC.A.g.g                                                                                              >  2:3437572/1‑101 (MQ=255)
  cttccttcATCTTGACGAAAAAGGGGTTG..GTTTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.g                                                                                              <  2:254680/101‑1 (MQ=255)
   ttccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.gg                                                                                             >  2:317858/1‑100 (MQ=255)
    tccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.ggc                                                                                            <  2:100720/101‑3 (MQ=255)
     ccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.ggca                                                                                           >  2:2594256/1‑98 (MQ=255)
     ccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.ggca                                                                                           <  1:212437/101‑4 (MQ=255)
     ccttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.ggca                                                                                           >  2:1360483/1‑98 (MQ=255)
      cttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.ggcag                                                                                          <  1:2634457/101‑5 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.g.ggcaga                                                                                         >  1:3758530/1‑96 (MQ=255)
        tcATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCagag                                                                                         <  2:151283/101‑1 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAgg                                                                                        >  2:2829472/1‑101 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAgg                                                                                        >  1:2567866/1‑101 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGCGATCAGACACGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.A.GAGCAGAgg                                                                                        >  2:2994687/1‑101 (MQ=255)
          aTCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGG.c                                                                                      <  1:3393230/101‑1 (MQ=255)
          aTCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGCCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGG.c                                                                                      >  1:2880758/1‑101 (MQ=255)
           tCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGG.cc                                                                                     >  1:4206135/1‑101 (MQ=255)
           tCTTGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGG.cc                                                                                     >  2:3802831/1‑101 (MQ=255)
             ttGACGAAAAAGGGGTTGGTG..TCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAA.G.GCAGAGG.cccc                                                                                   >  2:2805088/1‑101 (MQ=255)
                           gttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tt                                                                     >  2:2641597/7‑101 (MQ=255)
                            ttggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.ttt                                                                    <  1:365333/96‑2 (MQ=255)
                             tggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttg                                                                   <  2:1584331/97‑3 (MQ=255)
                             tggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttg                                                                   >  2:1528965/5‑99 (MQ=255)
                             tggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttg                                                                   <  2:733138/97‑3 (MQ=255)
                             gttggtggcggtcTTGGTGATCAGACAGGCCCCGGTTTCGACCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCAAG.tt                                                                     <  2:132175/93‑1 (MQ=255)
                              ggtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                  >  2:1438379/4‑98 (MQ=255)
                               gtg..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAG.tttgcg                                                                 >  1:675556/3‑97 (MQ=255)
                                 g..tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                >  2:2463731/1‑101 (MQ=255)
                                    tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                                               <  2:876534/101‑1 (MQ=255)
                                    tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                                               <  2:4118202/101‑1 (MQ=255)
                                    tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                                               >  2:2973716/1‑101 (MQ=255)
                                        tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                                                           <  2:2677671/91‑1 (MQ=255)
                                        tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                                                           >  2:2567001/11‑101 (MQ=255)
                                        tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                                                           >  2:2447474/11‑101 (MQ=255)
                                         cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                          >  2:3147019/10‑101 (MQ=255)
                                          ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                         <  1:3116696/93‑1 (MQ=255)
                                            ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                                                       >  2:3711022/7‑101 (MQ=255)
                                             gtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGt                                                      >  2:2870893/6‑101 (MQ=255)
                                              tgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                     >  2:363739/5‑101 (MQ=255)
                                              tgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                     >  1:4184398/5‑101 (MQ=255)
                                              tgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                     >  1:1323705/5‑101 (MQ=255)
                                              tgatcagacagCCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                     <  1:3436730/97‑1 (MQ=255)
                                               gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                    <  2:340980/98‑1 (MQ=255)
                                               gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                    >  2:3550312/4‑101 (MQ=255)
                                                  cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctctc                                                 <  1:1002926/101‑1 (MQ=255)
                                                  cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctctc                                                 <  1:3957878/101‑1 (MQ=255)
                                                   agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                                                >  2:2891814/1‑101 (MQ=255)
                                                     acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                                              <  2:2612827/101‑1 (MQ=255)
                                                     acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                                              >  2:224604/1‑101 (MQ=255)
                                                       agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                            <  2:2426434/101‑1 (MQ=255)
                                                       agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                            >  1:155635/1‑101 (MQ=255)
                                                        ggCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg                                           >  1:336838/1‑101 (MQ=255)
                                                             cGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                      <  1:4334797/101‑1 (MQ=255)
                                                              ggTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc                                     >  2:4216316/1‑101 (MQ=255)
                                                                tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                   >  2:1039917/1‑101 (MQ=255)
                                                                 ttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                  <  1:3611355/101‑1 (MQ=255)
                                                                  tCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTACACTGTc                                 <  2:2041351/101‑1 (MQ=255)
                                                                   cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                <  2:1573851/101‑1 (MQ=255)
                                                                      ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                             <  2:2702092/97‑1 (MQ=255)
                                                                      ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                             <  1:352681/97‑1 (MQ=255)
                                                                       cgaaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                            >  2:3351704/4‑101 (MQ=255)
                                                                        gaaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                           <  2:1231221/99‑1 (MQ=255)
                                                                         aaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                          >  1:17692/2‑101 (MQ=255)
                                                                         aaGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                          <  1:2829679/100‑1 (MQ=255)
                                                                          aGCCGCCCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctc                         >  2:1760415/1‑101 (MQ=255)
                                                                           gccgccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                        >  1:2348501/1‑101 (MQ=255)
                                                                           gccgccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                        >  1:4352538/1‑101 (MQ=255)
                                                                            ccgccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcct                       <  2:1961826/101‑1 (MQ=255)
                                                                             cgccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                      <  1:762365/101‑1 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                     >  2:637716/1‑101 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                     <  2:4163284/101‑1 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                     >  2:518173/1‑101 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                     <  1:3185317/101‑1 (MQ=255)
                                                                                cccGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGCCCCCTCCTCCgg                   <  1:2430061/101‑1 (MQ=255)
                                                                                 ccGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                  >  1:3134469/1‑101 (MQ=255)
                                                                                 ccGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                  >  1:1305332/1‑101 (MQ=255)
                                                                                 ccGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                  <  2:106398/101‑1 (MQ=255)
                                                                                 ccGGCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                  <  1:1447333/101‑1 (MQ=255)
                                                                                   ggCCAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc                >  2:1174779/1‑101 (MQ=255)
                                                                                     ccAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc              <  1:2861351/101‑1 (MQ=255)
                                                                                     ccAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc              <  2:1254643/101‑1 (MQ=255)
                                                                                     ccAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc              >  2:4027966/1‑101 (MQ=255)
                                                                                      cAGCGCGGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTGCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg             >  1:3799374/1‑101 (MQ=255)
                                                                                         cgcgGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc          <  1:2973937/101‑1 (MQ=255)
                                                                                         cgcgGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc          <  2:3614538/101‑1 (MQ=255)
                                                                                          gcgGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGCCa         >  1:306880/1‑101 (MQ=255)
                                                                                          gcgGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACa         <  1:636294/101‑1 (MQ=255)
                                                                                           cgGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa        >  1:1525836/1‑101 (MQ=255)
                                                                                           cgGAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa        <  1:2447631/101‑1 (MQ=255)
                                                                                            ggAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa       >  1:4442928/1‑101 (MQ=255)
                                                                                            ggAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa       <  1:363778/101‑1 (MQ=255)
                                                                                            ggAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa       <  1:3519170/101‑1 (MQ=255)
                                                                                             gAAAACGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGCGCTCGGACAAAg      >  2:3984061/1‑101 (MQ=255)
                                                                                              aaaaCGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt     <  1:2027648/101‑1 (MQ=255)
                                                                                                 aCGCC.AGG.GCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg  <  1:163939/101‑1 (MQ=255)
                                                                                                        |                                                                                               
ACCTTCCTTCATCTTGACGAAAA..GGGTTG..G..TCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCC.A.G.GCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTG  >  NC_014034/2177248‑2177434

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG