Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,590,199	+C	100%	intergenic (‑98/‑158)	glnE ← / → RCAP_rcc02415	glutamate‑ammonia‑ligase adenylyltransferase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,590,199	1	.→C	100.0%	242.4	65	intergenic (‑98/‑158)	glnE/RCAP_rcc02415	glutamate‑ammonia‑ligase adenylyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (64/1):  ref base (0/0):  total (64/1)

CATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.C.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTGA  >  NC_014034/2590099‑2590290                                                                                                        |                                                                                                 cATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAg.cg                                                                                                   >  1:3534084/1‑101 (MQ=255) cATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGGGCGG.GGGGCAg.cg                                                                                                   >  2:2916783/1‑101 (MQ=255)  aTGGCGCCAGCCTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAg.cg.c                                                                                                 <  2:2111390/101‑1 (MQ=255)  aTGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCGg.cg.c                                                                                                 >  1:82120/1‑101 (MQ=255)  aTGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAg.cg.c                                                                                                 <  1:1947440/101‑1 (MQ=255)   tGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.c                                                                                               >  1:3947979/1‑101 (MQ=255)     gcgcCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc                                                                                             >  1:992639/1‑101 (MQ=255)      cgcCAGCCTACCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.c                                                                                           <  1:2683795/101‑1 (MQ=255)      cgcCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.c                                                                                           >  1:3146320/1‑101 (MQ=255)      cgcCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.c                                                                                           <  2:3247176/101‑1 (MQ=255)      ccccAGCCTACCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.c                                                                                           <  1:1324193/99‑1 (MQ=255)       gcCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.cc                                                                                          >  2:4047258/1‑100 (MQ=255)       gcCAGACTAGCCGCCAAAAGCCCGGGGTCAGGGCCCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.cc                                                                                          <  2:1794443/101‑2 (MQ=37)       cccacccacgcccccGCAAGCCCCGGGTCAAGGCGCGCAAAGCCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.cc                                                                                          <  2:1692633/92‑2 (MQ=37)       cccAGACTAGCCCCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.cc                                                                                          <  1:1438271/100‑2 (MQ=255)         cAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.ccgc                                                                                        >  2:2897269/1‑98 (MQ=255)          aGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.ccgcc                                                                                       >  2:996062/1‑97 (MQ=255)          aGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGAGCGG.GGGGCAG.CG.c.ccc.ccgcc                                                                                       >  2:885674/1‑97 (MQ=255)           gACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.c.ccc.ccgccg                                                                                      >  2:1334438/1‑96 (MQ=255)             cTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCggg                                                                                  >  2:2588072/1‑101 (MQ=255)              tAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCgggc                                                                                 >  1:2103562/1‑100 (MQ=255)              tAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGt                                                                                 >  2:4048014/1‑101 (MQ=255)               aGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTc                                                                                >  2:1331730/1‑101 (MQ=255)               aGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTc                                                                                >  2:2243959/1‑101 (MQ=255)                  cgccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGGGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGACGcc                                                                             >  2:777501/1‑101 (MQ=38)                   gccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCt                                                                            >  2:4118466/1‑101 (MQ=255)                   gccgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCt                                                                            >  2:3177072/1‑101 (MQ=255)                     cgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCTTCg                                                                          >  1:2351424/1‑101 (MQ=255)                     cgcAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCg                                                                          >  1:4037785/1‑101 (MQ=255)                       cAAGCCCCGGGTGAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.CGCCC.C..G.CCGGGTCGCCTCGgc                                                                        >  1:2421423/1‑101 (MQ=255)                       cAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGgc                                                                        >  2:2381382/1‑101 (MQ=255)                            cccGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAg                                                                   >  2:29385/1‑101 (MQ=255)                              cGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGcc                                                                 >  2:312750/1‑101 (MQ=255)                              cGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.CGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGcc                                                                 >  1:3103559/1‑101 (MQ=255)                                ggTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCCAGCCGa                                                               >  1:3057874/1‑101 (MQ=255)                                    aaGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.C.CCCTC..G.CCGGGTCCCCTCGGCGCAAGCCGAGGCg                                                           >  1:296209/1‑101 (MQ=255)                                    aaGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGCGCGG.GGGGCTG.CGCC.CCC.C..G.CCGGGGCGGCCCGGCGCCAGCCGAGGCg                                                           >  1:835130/1‑101 (MQ=255)                                     aGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGa                                                          >  2:541429/1‑101 (MQ=255)                                      ggCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGaa                                                         >  2:3288174/1‑101 (MQ=255)                                        cgcgcAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAAt                                                       >  2:3941378/1‑101 (MQ=255)                                        cgcgcAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCCCGGCGCCAGCCGAGGCGaaaa                                                       >  1:11513/1‑100 (MQ=38)                                             aaaTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCACGCCGAGGCGAAAtctctc                                                  >  1:1756131/1‑101 (MQ=255)                                             aaaTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAAtctctc                                                  >  2:117111/1‑101 (MQ=255)                                               aTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGGAG.CGCC.CCC.C..G.CCGGGGCGCCTCGGCGCAAGCCGCGGCGAAATCTTTccc                                                >  2:3044332/1‑101 (MQ=25)                                               aTGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTccc                                                >  2:3611280/1‑101 (MQ=255)                                                   cccgccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGAc                                            >  1:2790897/1‑101 (MQ=255)                                                    ccgccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGCGGCGAAATCTCTcccccccc                                           >  1:3075744/1‑97 (MQ=37)                                                    ccgccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGAcc                                           >  1:1841028/1‑101 (MQ=255)                                                      gccccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGAcccc                                         >  2:1875895/1‑101 (MQ=255)                                                        cccTGTCATCTTGGCGCGAATATCCCGGGGGGTGGGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGGCGAGGCGAAATCTCTCCCCGACCCCtt                                       >  1:3680547/1‑101 (MQ=38)                                                        cccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCtt                                       >  2:3610189/1‑101 (MQ=255)                                                        cccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCtt                                       >  1:2063192/1‑101 (MQ=255)                                                        cccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCtt                                       >  1:1470856/1‑101 (MQ=255)                                                         ccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTg                                      >  1:1991355/1‑101 (MQ=255)                                                         ccTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTg                                      >  1:244043/1‑101 (MQ=255)                                                          cTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgc                                     >  1:98730/1‑101 (MQ=255)                                                          cTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgc                                     >  1:375968/1‑101 (MQ=255)                                                             tCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcggc                                  >  1:3513509/1‑101 (MQ=255)                                                              cATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcggcg                                 >  2:1468128/1‑101 (MQ=255)                                                              cATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTgcggcg                                 >  2:4213324/1‑101 (MQ=255)                                                                tcatGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCCCCCCGACCCCTTGCGGCGCa                               >  1:2553786/4‑101 (MQ=37)                                                                tCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGCCCCCttcccgccca                               >  2:2503653/1‑93 (MQ=25)                                                                tCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCa                               >  2:1444734/1‑101 (MQ=255)                                                                 cTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCGCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGgcgcca                              >  2:2616938/1‑99 (MQ=255)                                                                  ttGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAAt                             >  2:4203286/1‑101 (MQ=255)                                                                   tGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATg                            >  1:2387437/1‑101 (MQ=255)                                                                     gctgaactcccccGGGGGGCGCGG.GGCCTCC.CC.C.CCC.C..GTCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTa                          <  2:778601/91‑1 (MQ=21)                                                                     gcgcGAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTa                          >  2:3574719/1‑101 (MQ=255)                                                                      cgcgAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTaa                         >  1:1714135/1‑101 (MQ=255)                                                                        cgAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTaaaa                       >  2:2857024/1‑101 (MQ=255)                                                                        cgAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTaaaa                       >  1:3181704/1‑101 (MQ=255)                                                                         gAATATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTaaaaa                      >  2:646233/1‑101 (MQ=255)                                                                          aaTATCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCCATGTAAAAAt                     >  2:3143698/1‑101 (MQ=255)                                                                           atatCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATc                    >  2:2328178/1‑101 (MQ=255)                                                                             atCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCtt                  >  2:374158/1‑101 (MQ=255)                                                                              tCCCGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCttt                 >  1:1550771/1‑101 (MQ=255)                                                                              gcggggggggGGGGG.GGGCCCC.CC.C.CCC.C..GCCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCttt                 <  1:510940/97‑1 (MQ=25)                                                                              agcgggggggGGCGGTGGGGCCGCCC.C.CCC.C..GCCCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCt                   <  2:2111375/97‑1 (MQ=25)                                                                                ccGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTca               >  1:4033301/1‑101 (MQ=255)                                                                                 cGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCCAGCCGAGGCGAAATCTCTCCCCGACCCCCTGCGGCGCAATGTAAAAATCTTTcac              >  2:1636739/1‑101 (MQ=37)                                                                                 cGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTcac              >  1:1056543/1‑101 (MQ=255)                                                                                 cGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTcac              >  1:933287/1‑101 (MQ=255)                                                                                 cGGGGGGTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTcac              >  2:2384747/1‑101 (MQ=255)                                                                                   tcgggGGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCTACCCCTTGCGGCGCAATGTAAAAATCTTTCACAt            <  2:3971959/99‑1 (MQ=38)                                                                                      ggTGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCTACCCCTTGCGGCGCAATGTAAAAATCTTTCACAtaga         >  1:3972215/1‑98 (MQ=38)                                                                                        tGCGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGCAATCTCTCCCCCACCCCCTGCGGCGCACTGTAAAAATCTTTCACATccccc       >  1:937039/1‑100 (MQ=38)                                                                                        cgggg.ggggCGG.CCCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCct       <  1:1331827/98‑1 (MQ=38)                                                                                         gCGG.GGGGCCG.CC.C.CCC.CCGG.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCct       <  2:1412873/101‑1 (MQ=38)                                                                                          cGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCctct     >  1:894547/1‑101 (MQ=255)                                                                                          cGG.GGGGCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCctct     >  2:2176076/1‑101 (MQ=255)                                                                                           gg.ggggCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCtt    >  1:3579138/1‑101 (MQ=255)                                                                                            g.ggggCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCCAGCCGAGGCGAAATCTCTCCCCGACCCCCTGCGGCGGCATGTAAAAATCTTTTACATCCCCCCttt   >  1:1626936/1‑100 (MQ=25)                                                                                              ggggCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTga  >  2:4221913/1‑101 (MQ=255)                                                                                              ggggCAG.CGCC.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTga  >  2:600649/1‑101 (MQ=255)                                                                                                        |                                                                                                 CATGGCGCCAGACTAGCCGCCGCAAGCCCCGGGTCAAGGCGCGCAAATGCCCCGCCCCTGTCATCTTGGCGCGAATATCCCGGGGGGTGCGG.GGGGCAG.CG.C.CCC.C..G.CCGGGTCGCCTCGGCGCAAGCCGAGGCGAAATCTCTCCCCGACCCCTTGCGGCGCAATGTAAAAATCTTTCACATCCCCTCTTGA  >  NC_014034/2590099‑2590290 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG