BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	300,661	+G	2.1%	intergenic (+14/+130)	RCAP_rcc00263 → / ← RCAP_rcc00264	winged helix family two component transcriptional regulator/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	300,661	1	.→G	2.1%	4.7 ‑4.0 ‑0.0	221	intergenic (+14/+130)	RCAP_rcc00263/RCAP_rcc00264	winged helix family two component transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand): new base (4/0): ref base (78/138): total (83/138)
Fisher's exact test strand distribution p-value = 1.84e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.92e-01

Only 100 of 252 total aligned reads displayed.


TACCGGCTGCGCCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCGCCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAATATCCCGGGGGGA  >  NC_014034/300561‑300759


  ccGGCTGCGCCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..gg                                                                                                  <  1:3222510/101‑1 (MQ=255)


      cTGCGCCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGt                                                                                              <  1:2779167/101‑1 (MQ=255)


      cTGCGCCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGt                                                                                              <  2:4117938/101‑1 (MQ=255)


       tGCGCCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..ggggggt                                                                                             >  1:3383849/1‑99 (MQ=255)


       tGCGCCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..ggggggg                                                                                             >  2:3813446/1‑99 (MQ=255)


       tGCGCCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGtt                                                                                             >  1:1457412/1‑101 (MQ=255)


        gcgcCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGttt                                                                                            <  1:1649848/101‑1 (MQ=255)


        gcgcCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGttt                                                                                            <  1:2433080/101‑1 (MQ=255)


         cgcCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTc                                                                                           <  2:927957/101‑1 (MQ=255)


         cgcCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTc                                                                                           <  2:2961784/101‑1 (MQ=255)


          gcCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGGTTca                                                                                          >  2:4357208/1‑101 (MQ=255)


           ccAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTcac                                                                                         <  1:2158296/101‑1 (MQ=255)


           ccAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTcac                                                                                         <  2:3187758/101‑1 (MQ=255)


            cAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..gggggggtgtca                                                                                        >  2:4138117/1‑94 (MQ=255)


             agaagaTCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..gggggggttcacc                                                                                       >  1:1158755/1‑93 (MQ=255)


             agaagaTCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGgtttgcac                                                                                       >  1:1978808/1‑96 (MQ=255)


             agaagaTCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCacac                                                                                       <  1:1942069/101‑1 (MQ=255)


             agaagaTCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCacac                                                                                       >  2:4295882/1‑101 (MQ=255)


              gaagaTCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACAcc                                                                                      <  1:3003126/101‑1 (MQ=255)


              gaagaTCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGGTTGACAcc                                                                                      >  1:236397/1‑101 (MQ=255)


               aagaTCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACAccc                                                                                     <  2:1181853/101‑1 (MQ=255)


               aagaTCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACAccc                                                                                     <  2:2489291/101‑1 (MQ=255)


               aagaTCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACAccc                                                                                     <  1:2935865/101‑1 (MQ=255)


                  aTCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCcg                                                                                  <  1:2558683/101‑1 (MQ=255)


                      aGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCAcc                                                                              <  2:3268264/101‑1 (MQ=255)


                        cccGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GCG.GGGGGTTGCCCACCCCCCCCccc                                                                             >  2:2536124/1‑101 (MQ=255)


                           gATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGt                                                                         <  2:1555018/101‑1 (MQ=255)


                           gATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGt                                                                         <  2:4211220/101‑1 (MQ=255)


                             tCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTgg                                                                       >  1:4421070/1‑101 (MQ=255)


                             tCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGGTTCACACCCCCGCACCCCCGTgg                                                                       >  2:1445322/1‑101 (MQ=255)


                               cGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGa                                                                     <  1:4138377/101‑1 (MQ=255)


                               cGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGGTTCCCCCCCCCCCACCCCCCCGGGa                                                                     >  2:794249/1‑101 (MQ=255)


                                 tCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GCCGGGGGGTTTCCCCCCCCCCCCccccccgggcg                                                                     >  1:372396/1‑95 (MQ=255)


                                 tCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACGCCCCCGCACCCCCGTGGGata                                                                   <  1:2536287/101‑1 (MQ=255)


                                 tCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGata                                                                   <  2:665652/101‑1 (MQ=255)


                                 tCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGata                                                                   <  2:3888549/101‑1 (MQ=255)


                                  cGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGatat                                                                  >  1:3348367/1‑101 (MQ=255)


                                  cGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGGTTCACACCCCCGCACCCCCCTGGGatat                                                                  >  2:2857238/1‑101 (MQ=255)


                                    aaTGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATAttt                                                                <  1:3172710/101‑1 (MQ=255)


                                    aaTGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATAttt                                                                >  2:1276706/1‑101 (MQ=255)


                                       gcgcGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGGCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCa                                                             <  2:2201145/101‑1 (MQ=255)


                                         gcgGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGAGGC..GGGGGGGTTATACCCCCCCCCCCCCGCggggcactttcgc                                                            >  2:3963559/1‑91 (MQ=255)


                                          cgGCTTCTGATCCCGGAATCGGGCGGCTTCCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCa                                                          <  1:2015156/101‑1 (MQ=255)


                                           ggCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCaa                                                         <  2:3308440/101‑1 (MQ=255)


                                            gCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAg                                                        >  1:1374734/1‑101 (MQ=255)


                                            gCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAg                                                        <  1:2085319/101‑1 (MQ=255)


                                             cTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGGTTCACACCCCCCCACCCCCCCGGGGTATTTGCACCCAgg                                                       >  1:951429/1‑101 (MQ=255)


                                              ttCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGt                                                      <  1:2306614/101‑1 (MQ=255)


                                               tCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTg                                                     >  1:2107742/1‑101 (MQ=255)


                                                 tGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGaa                                                   <  2:2383554/101‑1 (MQ=255)


                                                 tGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGaa                                                   <  2:4077069/101‑1 (MQ=255)


                                                 tGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGGGaa                                                   >  2:2366199/1‑101 (MQ=255)


                                                    tCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAgg                                                <  2:3142408/101‑1 (MQ=255)


                                                      aCGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGc                                              <  2:377800/101‑1 (MQ=255)


                                                       cGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCa                                             <  2:3447403/101‑1 (MQ=255)


                                                       cGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGGGAAANNGcn                                             >  1:3912748/1‑100 (MQ=255)


                                                         gAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCAcc                                           <  1:3463049/101‑1 (MQ=255)


                                                         gAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGGTTTACACCCCCCCCCCCCCCGGGGATAATTGCACCAAAGGGAAAGGGGAcc                                           >  1:3741575/1‑101 (MQ=255)


                                                           aTCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCAcccc                                         <  2:1030542/101‑1 (MQ=255)


                                                           aTCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCAcccc                                         <  1:1775924/101‑1 (MQ=255)


                                                            tCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCCCCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCg                                        <  1:3563833/101‑1 (MQ=255)


                                                             cGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCgg                                       >  1:2395862/1‑101 (MQ=255)


                                                              gggCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCAGCCCtgg                                      >  1:2624177/1‑101 (MQ=255)


                                                              gggCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCggg                                      <  2:1101822/101‑1 (MQ=255)


                                                              gggCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCggg                                      <  2:170004/101‑1 (MQ=255)


                                                              gggCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCggg                                      <  1:3606836/101‑1 (MQ=255)


                                                               ggcggcTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGa                                     >  1:4087181/1‑101 (MQ=255)


                                                                 cggcTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTCGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATc                                   <  2:60966/101‑1 (MQ=255)


                                                                  ggcTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCt                                  <  2:275626/101‑1 (MQ=255)


                                                                   gcTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTg                                 >  1:1288950/1‑101 (MQ=255)


                                                                    cTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGc                                <  1:1524266/101‑1 (MQ=255)


                                                                    cTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGc                                >  2:3642707/1‑101 (MQ=255)


                                                                    cTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGc                                <  2:1164580/101‑1 (MQ=255)


                                                                    cTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGc                                >  2:1136243/1‑101 (MQ=255)


                                                                      aCCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCtt                              <  1:4278638/101‑1 (MQ=255)


                                                                       ccGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCttt                             <  2:2772558/101‑1 (MQ=255)


                                                                        cGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGCTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTc                            <  1:3813699/101‑1 (MQ=255)


                                                                            tGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCt                        >  2:2880667/1‑101 (MQ=255)


                                                                            tGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCt                        >  1:404424/1‑101 (MQ=255)


                                                                            tGGTCGCCTGAGACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCt                        <  2:1901197/101‑1 (MQ=255)


                                                                             ggTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCtt                       <  2:372357/101‑1 (MQ=255)


                                                                             ggTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCtt                       <  1:1062743/101‑1 (MQ=255)


                                                                             ggTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCtt                       <  2:2094815/101‑1 (MQ=255)


                                                                             ggTCGCCTGATACCGCGCAGAA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGAACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCtt                       >  2:600301/1‑101 (MQ=255)


                                                                              gTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTg                      <  1:849791/101‑1 (MQ=255)


                                                                              gTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTg                      <  2:323239/101‑1 (MQ=255)


                                                                               gcGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTgg                     <  1:1338635/100‑1 (MQ=255)


                                                                                 gCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTg                   <  2:196141/101‑1 (MQ=255)


                                                                                  ccTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGa                  <  1:414777/101‑1 (MQ=255)


                                                                                   cTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGaa                 <  2:1639698/101‑1 (MQ=255)


                                                                                    tGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGaaa                >  1:4204933/1‑101 (MQ=255)


                                                                                        aCCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAatat            <  2:2929821/101‑1 (MQ=255)


                                                                                         ccGCGCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAATATc           <  1:2890050/101‑1 (MQ=255)


                                                                                          cgcgCAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAATATcc          >  2:444388/1‑101 (MQ=255)


                                                                                           gcgcAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAATATccc         <  1:4357479/101‑1 (MQ=255)


                                                                                             gcAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAATATCCCgg       <  1:710114/101‑1 (MQ=255)


                                                                                             gcAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAATATCCCgg       >  1:3945254/1‑101 (MQ=255)


                                                                                              cAGGA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAATATCCCggg      >  1:3636628/1‑101 (MQ=255)


                                                                                                ggA.GC..GGGGGTTTCACACCCCCGCACCCCCGTGCGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAATATCCCggggg    <  1:3789206/101‑1 (MQ=255)


                                                                                                  a.GC..GGGGGTTTCACACCCCCGCACCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAATATCCCGGGGGGa  <  1:181036/101‑1 (MQ=255)


TACCGGCTGCGCCAGAAGATCGAGCCCGATCCGTCGAATGCGCGGCTTCTGATCACGGAATCGGGCGGCTACCGTCTGGTCGCCTGATACCGCGCAGGA.GC..GGGGGTTTCACACCCCCGCGCCCCCGTGGGATATTTGCACCAAGGTGAAAGGGCACCCCGGGATCTGCTTTCACCTTGGTGAAAATATCCCGGGGGGA  >  NC_014034/300561‑300759

Base quality scores:


ATCG

< 3 ≤


ATCG

< 19 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG