BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,461,398	+G	94.0%	intergenic (‑108/+22)	RCAP_rcc03260 ← / ← RCAP_rcc03261	NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,461,398	1	.→G	94.0%	18.1 ‑2.8 ‑0.1	133	intergenic (‑108/+22)	RCAP_rcc03260/RCAP_rcc03261	NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand): new base (62/63): ref base (7/1): total (69/64)
Fisher's exact test strand distribution p-value = 6.36e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.97e-01

Only 100 of 143 total aligned reads displayed.


CGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGA  >  NC_014034/3461307‑3461492


cGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCGGgcccc.caaccccgg                                                                                       >  1:3733976/1‑93 (MQ=255)


    cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                                               <  2:2107219/101‑1 (MQ=255)


     ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                                              <  2:4343925/101‑1 (MQ=255)


     ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                                              <  2:2858808/101‑1 (MQ=255)


     ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                                              >  2:533440/1‑101 (MQ=255)


       gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                                                            <  2:1952006/101‑1 (MQ=255)


        cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                                                           >  2:2947658/1‑101 (MQ=255)


        cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                                                           <  1:2517397/101‑1 (MQ=255)


        cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                                                           <  1:663438/101‑1 (MQ=255)


         aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                                                          >  2:202186/1‑101 (MQ=255)


         aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                                                          <  1:3417163/101‑1 (MQ=255)


          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                                                         <  2:4437657/101‑1 (MQ=255)


          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GC..AGCCAACC.GGCCGCGCCCCCAACCCCGGTCGTTCGATc                                                                         >  1:3211255/1‑101 (MQ=255)


           cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                                                        >  1:3494710/1‑101 (MQ=255)


           cTTAGAAAGATGCGCGGGGCCGCCCCGCCGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GGCCCAGCC.ACCCCGCCG...CG..CCCCCAACCCCGGTCGTTCGATCa                                                                        <  2:567523/101‑1 (MQ=255)


             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      <  1:520629/101‑1 (MQ=255)


             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      >  1:3730562/1‑101 (MQ=255)


             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      <  1:1442277/101‑1 (MQ=255)


              aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCG...CGC.CCCCC.ACCCCGGTCGTTCGATCAGtt                                                                     >  1:4339176/1‑101 (MQ=255)


              aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                     <  2:3999632/101‑1 (MQ=255)


              aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                     >  1:3207027/1‑101 (MQ=255)


              aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGACGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                     >  2:1817176/1‑101 (MQ=255)


               gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                                                    >  2:3800219/1‑101 (MQ=255)


                 aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                                                  <  2:3955126/101‑1 (MQ=255)


                   gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                                >  2:2197475/1‑101 (MQ=255)


                   gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                                >  1:2642963/1‑101 (MQ=255)


                    aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                                               >  1:566592/1‑101 (MQ=255)


                    aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                                               >  2:2675822/1‑101 (MQ=255)


                    aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                                               >  1:1754427/1‑101 (MQ=255)


                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                              >  2:3434680/1‑101 (MQ=255)


                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                              <  2:2349676/101‑1 (MQ=255)


                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                              <  2:51690/101‑1 (MQ=255)


                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                              <  2:1244882/101‑1 (MQ=255)


                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                              >  1:4204945/1‑101 (MQ=255)


                      gCACGGGCGCGGTCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                                                             >  1:2424205/1‑101 (MQ=255)


                      gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                                                             <  1:2848254/101‑1 (MQ=255)


                      gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGGCGGTCGATCAGTTCTTCCcga                                                             >  2:2722907/1‑101 (MQ=255)


                       cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                            <  1:4440837/101‑1 (MQ=255)


                       cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                            <  2:2192517/101‑1 (MQ=255)


                       cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                            <  2:3474612/101‑1 (MQ=255)


                        aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                                                           >  1:1807860/1‑101 (MQ=255)


                        aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                                                           >  2:3756480/1‑101 (MQ=255)


                         cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGCTCAGTTCTTGCcgacga                                                          >  1:743932/1‑101 (MQ=255)


                          gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                         >  2:2858817/1‑101 (MQ=255)


                           ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                                                        >  1:821679/1‑101 (MQ=255)


                            gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCTGGTCGTTCGATCAGTTCTTGCCGACGATGa                                                       <  2:3976994/101‑1 (MQ=255)


                              gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                                                     <  1:4051574/101‑1 (MQ=255)


                              gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                                                     <  2:698467/101‑1 (MQ=255)


                                ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                                   >  2:2125424/1‑101 (MQ=255)


                                ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                                   >  2:1009380/1‑101 (MQ=255)


                                ggCCAGACGCAGTTTGGCCCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                                   <  1:1693145/101‑1 (MQ=255)


                                 gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.A.G.GG.GC.GC..AACCAACC.GGCGGCGGCCCCAACGCCGGTCGTTCGCTCAGTTCTTGCCGCCGCTGACGTCa                                                  >  2:2178736/1‑101 (MQ=255)


                                  ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTccg                                                 >  2:3184684/1‑99 (MQ=255)


                                   cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                                <  1:3936270/101‑1 (MQ=255)


                                   cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                                <  1:1596684/101‑1 (MQ=255)


                                    aGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                               <  1:3069809/101‑1 (MQ=255)


                                    aGACGAAGTTTGGCGCAGGGCCAAAGGAA.G.T.GT.GC.GCAGGCAC..CCGGGCGCG.CCCCCAACCCCGGTCGTTCTATCAGTTCTTTGCGACGATGACGTCagag                                               >  2:738617/1‑101 (MQ=255)


                                     gACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                                              >  1:276565/1‑101 (MQ=255)


                                        gCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt                                           >  1:4432896/1‑101 (MQ=255)


                                         cAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt                                          <  1:274372/101‑1 (MQ=255)


                                         cAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt                                          >  2:1557234/1‑101 (MQ=255)


                                          aGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                         <  1:913877/101‑1 (MQ=255)


                                           gTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga                                        >  1:1232571/1‑101 (MQ=255)


                                           gTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga                                        >  1:1066661/1‑101 (MQ=255)


                                           gTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga                                        <  1:1243131/101‑1 (MQ=255)


                                            tttGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat                                       <  2:154270/101‑1 (MQ=255)


                                              tGGCGCAGGGCCAAAGGAAGGAG.GC.GCAGCC.ACCC.....GGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                     >  1:2538275/1‑101 (MQ=255)


                                              tGGCGCAGGGCCAAAGGAAGG.GAGC.GCAGCC.ACCCTGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                     <  2:743852/101‑1 (MQ=255)


                                              tGGCGCAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                     >  1:270951/1‑101 (MQ=255)


                                              tGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                     <  2:2943594/101‑1 (MQ=255)


                                               ggCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                                    <  2:2798682/101‑1 (MQ=255)


                                                 cgcAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg                                  >  1:2120997/1‑101 (MQ=255)


                                                 cgcAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg                                  >  2:2880198/1‑101 (MQ=255)


                                                  gcAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc                                 >  2:4175234/1‑101 (MQ=255)


                                                  gcAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc                                 >  1:3375662/1‑101 (MQ=255)


                                                                         gggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGcc                  <  2:1807741/91‑1 (MQ=255)


                                                                         gggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGcc                  >  1:423160/11‑101 (MQ=255)


                                                                         gggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGcc                  <  1:497979/91‑1 (MQ=255)


                                                                          ggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCg                 <  1:1558551/92‑1 (MQ=255)


                                                                             gcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAcc              <  1:3461341/95‑1 (MQ=255)


                                                                             gcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAcc              <  2:566540/95‑1 (MQ=255)


                                                                              cgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCt             >  1:676497/6‑101 (MQ=255)


                                                                               gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt            >  1:1869076/5‑101 (MQ=255)


                                                                               gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt            >  2:1701005/5‑101 (MQ=255)


                                                                                cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc           <  2:66609/98‑1 (MQ=255)


                                                                                cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc           <  2:4204684/98‑1 (MQ=255)


                                                                                 agccccccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa          <  2:179310/93‑1 (MQ=255)


                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTcg          >  1:2125543/3‑100 (MQ=255)


                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa          <  1:2255717/99‑1 (MQ=255)


                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa          >  2:4320305/3‑101 (MQ=255)


                                                                                 agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa          <  1:3434919/99‑1 (MQ=255)


                                                                                  gccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAg         <  2:3514315/100‑1 (MQ=255)


                                                                                   ccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgc        >  2:1105312/1‑101 (MQ=255)


                                                                                       ccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCa    <  2:1738253/99‑1 (MQ=255)


                                                                                        cggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAg   <  1:734021/100‑1 (MQ=255)


                                                                                        cggCCGCGCCCCCAACCCCGGCCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGCCGCCGACCTTCAGCGcccg   >  1:2245322/2‑99 (MQ=255)


                                                                                         ggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGa  <  1:3017301/101‑1 (MQ=255)


                                                                                         ggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGa  <  2:3089388/101‑1 (MQ=255)


                                                                                         ggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGa  >  2:3666503/1‑101 (MQ=255)


                                                                                         ggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGa  >  2:3564034/1‑101 (MQ=255)


CGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGA  >  NC_014034/3461307‑3461492

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG