Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,701,433 +GGGCTTC 100% intergenic (+10/+39) grpE → / ← mutS GrpE protein/DNA mismatch repair protein MutS

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,701,4331.→G100.0% 103.6 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/39):  ref base (0/0):  total (0/40)
*NC_0140343,701,4332.→G100.0% 109.3 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)
*NC_0140343,701,4333.→G100.0% 104.8 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)
*NC_0140343,701,4334.→C100.0% 107.7 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)
*NC_0140343,701,4335.→T100.0% 109.1 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)
*NC_0140343,701,4336.→T100.0% 108.2 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)
*NC_0140343,701,4337.→C100.0% 107.1 40intergenic (+10/+39)grpE/mutSGrpE protein/DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  new base (0/40):  ref base (0/0):  total (0/40)

CATCATTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGG.......GCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGC  >  NC_014034/3701333‑3701498
                                                                                                     |||||||                                                                 
catcatTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg                                                                          >  2:1590595/1‑101 (MQ=255)
catcatTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg                                                                          <  2:2093882/101‑1 (MQ=255)
catcatTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg                                                                          <  1:3025972/101‑1 (MQ=255)
 atcatTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......g                                                                  <  2:1311886/101‑1 (MQ=255)
    atTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGACAGCCTGAGGCAGGCCgg.......gggc                                                               <  1:2545443/101‑4 (MQ=255)
    atTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggc                                                               <  2:1890935/101‑4 (MQ=255)
    atTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggc                                                               <  1:3442681/101‑4 (MQ=255)
    atTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggc                                                               <  1:307957/101‑4 (MQ=255)
      tCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggctt                                                             <  2:1296339/101‑6 (MQ=255)
      tCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggctt                                                             <  2:1125966/101‑6 (MQ=255)
      tCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggctt                                                             <  1:872921/101‑6 (MQ=255)
      tCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggctt                                                             <  1:412684/101‑6 (MQ=255)
      tCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggctt                                                             <  1:1503923/101‑6 (MQ=255)
      tCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggctt                                                             <  1:1590701/101‑6 (MQ=255)
       cAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttc                                                            <  1:433753/101‑7 (MQ=255)
       cAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttc                                                            <  1:1699272/101‑7 (MQ=255)
       cAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttc                                                            <  1:1563591/101‑7 (MQ=255)
       cAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttc                                                            <  2:3180379/101‑7 (MQ=255)
       cAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttc                                                            <  1:3248898/101‑7 (MQ=255)
       cAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttc                                                            <  2:3014869/101‑7 (MQ=255)
       cAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttc                                                            <  2:3002293/101‑7 (MQ=255)
          gTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCgg.......gggcttcgcc                                                         <  2:1420685/101‑10 (MQ=255)
                               ctgcACGGCCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATAtttt                                           <  1:3164676/101‑1 (MQ=21)
                               ctgcACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATAtttt                                           <  2:2659946/101‑1 (MQ=21)
                               ctgcACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATAtttt                                           <  2:2180082/101‑1 (MQ=21)
                               ctgcACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATAtttt                                           <  1:417735/101‑1 (MQ=21)
                               ctgcACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATAtttt                                           <  1:2794739/101‑1 (MQ=21)
                                tgcACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTc                                          <  1:3615165/101‑1 (MQ=21)
                                tgcACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTc                                          <  1:961114/101‑1 (MQ=21)
                                 gcACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCa                                         <  1:728953/101‑1 (MQ=21)
                                 gcACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCa                                         <  2:643228/101‑1 (MQ=21)
                                 gcACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCa                                         <  2:2968818/101‑1 (MQ=21)
                                  cACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAg                                        <  1:3527149/101‑1 (MQ=21)
                                         gTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATa                                 <  1:4162330/101‑1 (MQ=21)
                                         gTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATa                                 <  2:2321284/101‑1 (MQ=21)
                                          gctgctgCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATaa                                <  2:3302518/100‑1 (MQ=21)
                                           ctgctgCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATaaa                               <  1:604866/101‑1 (MQ=21)
                                             gctgcGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGa                             <  1:2140948/101‑1 (MQ=21)
                                             gctgcCTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGa                             <  2:1609939/101‑1 (MQ=21)
                                              ctgcTTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGAt                            <  2:70770/101‑1 (MQ=21)
                                              ctgcGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGAt                            <  1:2736718/101‑1 (MQ=21)
                                               tgcGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATc                           <  2:282858/101‑1 (MQ=21)
                                                gcGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCCAGATAAAGATCa                          <  1:2423954/101‑1 (MQ=21)
                                                 cGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAATCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAg                         <  2:3009973/101‑1 (MQ=21)
                                                  gTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGt                        <  1:1797108/101‑1 (MQ=21)
                                                  gTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGt                        <  1:2118511/101‑1 (MQ=21)
                                                   tCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCCAGATAAAGATCAGTc                       <  1:3752378/101‑1 (MQ=21)
                                                   tCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGAGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTc                       <  1:391305/101‑1 (MQ=25)
                                                     cGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCtt                     <  1:2734890/101‑1 (MQ=21)
                                                      ggCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCttt                    <  1:4238643/101‑1 (MQ=21)
                                                       gCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTCTc                   <  1:2557220/101‑1 (MQ=21)
                                                          aaaGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGa                <  2:2840976/101‑1 (MQ=21)
                                                          aaaGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGa                <  2:3141691/101‑1 (MQ=21)
                                                          aaaGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGa                <  2:372193/101‑1 (MQ=21)
                                                           aaGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGaa               <  1:545032/101‑1 (MQ=21)
                                                             gTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAgg             <  1:3868319/101‑1 (MQ=21)
                                                             gTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAgg             <  2:3891483/101‑1 (MQ=21)
                                                               cGGCGTGAGTTCTATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGgc           <  2:3893446/101‑1 (MQ=21)
                                                                ggCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGgcg          <  1:4314031/101‑1 (MQ=21)
                                                                     gAGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAg     <  1:2035393/101‑1 (MQ=21)
                                                                      aGTTCGATGCCGAAAGCCTGAGGCAGGCCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAgg    <  1:501886/101‑1 (MQ=21)
                                                                        ttCGATGCCGAAAGCCTGAGGCAGGGCGGGGGCTTCGCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGc  <  1:3234864/101‑1 (MQ=21)
                                                                                                     |||||||                                                                 
CATCATTCAGGTGATGGACGACGGCTTCCTGCTGCACGACCGTCTGCTGCGTCCGGCGAAAGTCGGCGTGAGTTCGATGCCGAAAGCCTGAGGCAGGCCGG.......GCCCCCGGACCCCCAGGATATTTTCAGCAAGATAAAGATCAGTCTTTCAGAAGGGCGCGCAGGGC  >  NC_014034/3701333‑3701498

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG