New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_014034	= 1224266	202 (0.990)	36 (0.180)	13/176	24.2	0.17	intergenic (‑138/+31)	hisF/hisA	imidazole glycerol phosphate synthase subunit HisF/1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino] imidazole‑4‑carboxamide isomerase
	?	NC_014034	3011299 =	148 (0.650)					intergenic (+98/+44)	purK/RCAP_rcc02822	phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase
Rejected: Position hash score below cutoff.

GCGTCTTGCGGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGG....................................................................................................  >  NC_014034/1224158‑1224266 ...................................................................................................TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACCGGCTCGAACT  >  NC_014034/3011299‑3011398                                                                                                                                                                                                                            GGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGA                                                                                                     <  1:3659977/101‑1 (MQ=1)          GGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGA                                                                                                     <  2:2285462/101‑1 (MQ=1)          GGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGA                                                                                                     <  2:2540591/101‑1 (MQ=1)          GGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGA                                                                                                     <  1:2524859/101‑1 (MQ=1)           GCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGAG                                                                                                    <  1:273974/101‑1 (MQ=1)                                                                                  TTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCG                             >  2:4439637/1‑101 (MQ=255)                                                                                  TTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCG                             >  2:2491595/1‑101 (MQ=255)                                                                                   TCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGC                            >  1:3721980/1‑101 (MQ=9)                                                                                   TCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGC                            >  1:3638370/1‑101 (MQ=9)                                                                                   TCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGC                            >  1:1922505/1‑101 (MQ=9)                                                                                   TCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGC                            >  1:1504774/1‑101 (MQ=9)                                                                                     accttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGC                          >  2:3402938/9‑101 (MQ=2)                                                                                      ccttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCC                         >  1:2973579/8‑101 (MQ=2)                                                                                       cttgggACAAATATCCCGGGGGGAGACTTGCCGGAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCC                        >  1:371499/7‑101 (MQ=2)                                                                                       cttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCCGCCGCTGCAAGGCCGCGCCC                        >  2:4417777/7‑101 (MQ=2)                                                                                       cttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCCGCCGCTGCAAGGCCGCGCCC                        >  1:4376899/7‑101 (MQ=2)                                                                                        ttgggaaAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTCCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCC                       <  2:1870437/94‑1 (MQ=0)                                                                                        ttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCC                       >  1:1916686/6‑101 (MQ=11)                                                                                         tgggaaAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      <  2:2376995/95‑1 (MQ=0)                                                                                         tgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      >  2:580321/5‑101 (MQ=11)                                                                                         tgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      >  2:18417/5‑101 (MQ=11)                                                                                         tgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      <  1:998754/97‑1 (MQ=11)                                                                                         tgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      >  1:2937364/5‑101 (MQ=11)                                                                                         tgggACAAATACCCGGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      <  2:3187602/97‑1 (MQ=2)                                                                                         tggaACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      <  2:3881960/97‑1 (MQ=11)                                                                                          gggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG                     <  1:4170000/98‑1 (MQ=11)                                                                                           ggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGC                    >  1:2345687/3‑101 (MQ=11)                                                                                            gACAAATACCCGGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCCCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCC                   <  2:3623407/100‑1 (MQ=2)                                                                                             ACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCC                  >  2:1861339/1‑101 (MQ=11)                                                                                             ACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCCCTGCACGGCCGCGCCCCAcgcc                  >  1:232493/1‑97 (MQ=2)                                                                                              CAAATACCCGGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCG                 <  1:245215/101‑1 (MQ=0)                                                                                               AAATATCGCGGGGGGAGACTGGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCCCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGG                <  1:2147362/101‑1 (MQ=0)                                                                                               AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGG                >  1:2808094/1‑101 (MQ=1)                                                                                               AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCCCGCCCCAGagatc                >  2:2599721/1‑96 (MQ=0)                                                                                                AATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGC               >  1:719590/1‑101 (MQ=1)                                                                                                AATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGC               >  2:1315440/1‑101 (MQ=1)                                                                                                 ATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCGCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCc              >  2:3855287/1‑100 (MQ=1)                                                                                                  TATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCAC             >  1:3149632/1‑101 (MQ=1)                                                                                                  TATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCAC             >  1:20426/1‑101 (MQ=1)                                                                                                   ATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCCGCCCGGCACC            >  2:1534682/1‑101 (MQ=1)                                                                                                   ATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGATGCAAGGCCGCGCCCCAGCCCGGGACC            >  2:2792568/1‑101 (MQ=0)                                                                                                                                                                                                                   GCGTCTTGCGGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGG....................................................................................................  >  NC_014034/1224158‑1224266 ...................................................................................................TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACCGGCTCGAACT  >  NC_014034/3011299‑3011398 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG