Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,879 C→A 2.9% intergenic (+66/+91) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8790C→A 2.9% 3.6 ‑2.1 ‑0.9138intergenic (+66/+91)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (65/69):  total (65/73)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.20e-01

Only 100 of 155 total aligned reads displayed.

GCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCA  >  NC_014034/1303782‑1303972
                                                                                                      |                                                                                                        
gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCt                                                                                                         <  1:1209386/101‑1 (MQ=37)
gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCt                                                                                                         <  1:1717887/101‑1 (MQ=37)
gcgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCt                                                                                                         >  2:2713562/1‑101 (MQ=37)
 cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCtt                                                                                                        >  2:1236502/1‑101 (MQ=37)
 cgAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCGCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCCtt                                                                                                        >  2:2516216/1‑101 (MQ=25)
  gAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCttt                                                                                                       >  2:954734/1‑101 (MQ=37)
   aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                                      >  2:3716029/1‑101 (MQ=37)
   aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                                      >  1:1964968/1‑101 (MQ=37)
   aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTc                                                                                                      <  2:1153367/101‑1 (MQ=37)
   aaaCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAA.CCCATCATTTc                                                                                                      >  2:1634156/1‑101 (MQ=25)
     aCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAc                                                                                                    <  2:1520131/101‑1 (MQ=37)
      cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCAcc                                                                                                   <  1:2909380/101‑1 (MQ=37)
       ccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCt                                                                                                  <  1:268955/101‑1 (MQ=37)
         tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTg                                                                                                <  1:1029661/101‑1 (MQ=37)
         tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTg                                                                                                <  1:2340026/101‑1 (MQ=37)
          gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGc                                                                                               >  1:4069879/1‑101 (MQ=37)
           cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGcc                                                                                              <  2:709830/101‑1 (MQ=37)
           cgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGcc                                                                                              >  2:268817/1‑101 (MQ=37)
            gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCg                                                                                             >  2:198702/1‑101 (MQ=37)
            gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCg                                                                                             >  1:381672/1‑101 (MQ=37)
             cgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGc                                                                                            <  1:2484888/101‑1 (MQ=37)
                cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                                                                                         <  1:3252659/101‑1 (MQ=37)
                cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCaaa                                                                                         >  2:676889/1‑101 (MQ=37)
                 gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAat                                                                                        <  1:548970/101‑1 (MQ=37)
                 gTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGAAAat                                                                                        <  2:603395/101‑1 (MQ=25)
                   ggTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCTCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAatat                                                                                      <  1:4408920/101‑1 (MQ=37)
                   ggTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAatat                                                                                      >  1:1970261/1‑101 (MQ=37)
                    gTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                                                                                     <  2:4366074/101‑1 (MQ=37)
                    gTCAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATc                                                                                     <  1:4091011/101‑1 (MQ=37)
                      cAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATccc                                                                                   <  2:1415316/101‑1 (MQ=37)
                      cAGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCCCAAATATccc                                                                                   >  1:4031346/1‑101 (MQ=25)
                       aGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCg                                                                                  <  2:2251175/101‑1 (MQ=37)
                        gCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCCCCCCCGGGGTATTTGGGGCAAGAT.AAAC.CCCCTCCTTTCACCTTGCCGCAAATATCCCgg                                                                                 >  2:4200577/1‑101 (MQ=25)
                         cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                                >  2:3450970/1‑101 (MQ=25)
                         cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGAGATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                                >  1:3764368/1‑101 (MQ=37)
                          gTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg                                                                               >  2:1044292/1‑101 (MQ=37)
                          gTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg                                                                               <  1:2713752/101‑1 (MQ=37)
                           tttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                              <  2:2141317/101‑1 (MQ=37)
                            ttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggggg                                                                             >  2:3877198/1‑101 (MQ=25)
                             tAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGt                                                                            <  1:3450426/101‑1 (MQ=37)
                              cggacggggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAAt                                                                        <  1:3652129/95‑1 (MQ=2)
                              aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                           >  1:2803911/1‑101 (MQ=37)
                              aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                           >  2:3034117/1‑101 (MQ=25)
                               ggCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa                                                                          <  1:94295/101‑1 (MQ=37)
                                gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                         <  2:2298036/101‑1 (MQ=37)
                                gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGaa                                                                         >  1:3093844/1‑101 (MQ=25)
                                 cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGGCAGAT.AAAC.CCCATACTTTCACCTTGCCGCAAATATTCCGGGGGGGGact                                                                        >  2:4417341/1‑99 (MQ=21)
                                 cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                                        <  1:2281954/101‑1 (MQ=37)
                                 acggggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTgg                                                                     <  2:3002218/98‑1 (MQ=2)
                                  ctgggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                       <  1:2093029/99‑1 (MQ=25)
                                  aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCGCCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                       <  1:2705808/101‑1 (MQ=25)
                                  aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                       <  1:3714195/101‑1 (MQ=37)
                                  aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                       <  2:537103/101‑1 (MQ=37)
                                  aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAAtt                                                                       >  1:953618/1‑101 (MQ=25)
                                  aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATACCTGTTCCTTGCCACAAATATGCCgggggggcaact                                                                       >  1:3837157/1‑95 (MQ=12)
                                   aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                                      <  2:3421725/101‑1 (MQ=37)
                                   aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                                      <  2:3443337/101‑1 (MQ=37)
                                   aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                                      <  2:2710675/101‑1 (MQ=37)
                                   aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAGTg                                                                      >  2:3748250/1‑101 (MQ=25)
                                    gggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                                  <  2:4436892/101‑2 (MQ=2)
                                    gggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                                  <  2:4155742/101‑2 (MQ=2)
                                    gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                     >  2:3503051/1‑101 (MQ=37)
                                     ggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgc                                                                    >  2:2568582/1‑101 (MQ=37)
                                      gggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc                                                                   >  1:423240/1‑101 (MQ=37)
                                                                             tttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                         <  1:954819/101‑1 (MQ=14)
                                                                              ttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcgg                        <  2:4304026/101‑1 (MQ=14)
                                                                               tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGTGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                       >  2:300071/1‑101 (MQ=9)
                                                                               tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                       >  2:2885445/1‑101 (MQ=14)
                                                                               tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                       <  2:1970132/101‑1 (MQ=14)
                                                                               tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                       <  2:3221761/101‑1 (MQ=14)
                                                                               tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                       >  1:3138648/1‑101 (MQ=14)
                                                                                 gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                     >  2:4154139/1‑101 (MQ=14)
                                                                                    cAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                  >  2:3888972/1‑101 (MQ=14)
                                                                                    cAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                  >  1:3356076/1‑101 (MQ=14)
                                                                                     aaGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag                 >  1:210835/1‑101 (MQ=14)
                                                                                     aaGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag                 >  1:777618/1‑101 (MQ=14)
                                                                                      aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                >  1:2649734/1‑101 (MQ=14)
                                                                                      aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                >  2:1255868/1‑101 (MQ=14)
                                                                                      aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                >  2:2472281/1‑101 (MQ=14)
                                                                                      aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                >  2:3254270/1‑101 (MQ=14)
                                                                                        aT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggc              >  1:93677/1‑101 (MQ=14)
                                                                                           aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg            >  2:560068/1‑101 (MQ=14)
                                                                                           aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg            >  1:1427834/1‑101 (MQ=14)
                                                                                           aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg            >  2:423736/1‑101 (MQ=14)
                                                                                            aaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc           >  2:1781408/1‑101 (MQ=14)
                                                                                             aC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgca          >  1:3863071/1‑101 (MQ=14)
                                                                                              c.cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc         <  2:3813664/101‑1 (MQ=14)
                                                                                              c.cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc         >  2:2303029/1‑101 (MQ=14)
                                                                                                cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc        >  1:2702403/1‑101 (MQ=14)
                                                                                                 ccATCCTGTCACCTGGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTGCGGGGCGGGCCGCAGGGCCGCAccc       <  1:4417613/101‑1 (MQ=9)
                                                                                                  cctCCTTTCACCTTGCCGCAAATTTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt      <  2:4031089/99‑1 (MQ=9)
                                                                                                  cATCCTTTCACCTTGCCTTAAATATCCCGGGGGGTGAACTGGCC....GAAGACCAAGAGGGGGGCAGCGCCCCTTTTTCGGGGGGGGCCGCAGGGCCGCACCCt      <  2:2638958/101‑1 (MQ=9)
                                                                                                  cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt      <  1:549071/101‑1 (MQ=14)
                                                                                                    tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGt    >  1:453942/1‑101 (MQ=14)
                                                                                                     ccTTTCGCCTGGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc   <  2:4102175/101‑1 (MQ=9)
                                                                                                     ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc   >  2:2882358/1‑101 (MQ=14)
                                                                                                     ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc   >  1:2939892/1‑101 (MQ=14)
                                                                                                      cTTTCACCTTGCCGCAAATGTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa  <  2:4261764/101‑1 (MQ=9)
                                                                                                      cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa  >  2:4376887/1‑101 (MQ=14)
                                                                                                      cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa  <  2:1670313/101‑1 (MQ=14)
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GCGAAACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCA  >  NC_014034/1303782‑1303972

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG