Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,552 C→G 32.1% intergenic (+364/‑567) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,5520C→G32.1% 286.5 ‑2.2 ‑0.8317intergenic (+364/‑567)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (44/56):  ref base (115/98):  total (161/156)
Fisher's exact test strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.74e-01

Only 100 of 340 total aligned reads displayed.

GCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1395452‑1395652
                                                                                                      |                                                                                                     
gCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.c                                                                                                       >  2:3692906/1‑101 (MQ=255)
 cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.cc                                                                                                      <  1:3579236/101‑1 (MQ=255)
  gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCa                                                                                                     <  2:4178411/101‑1 (MQ=255)
   tGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAg                                                                                                    >  1:145093/1‑101 (MQ=255)
    ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGGTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGc                                                                                                   <  1:584867/101‑1 (MQ=255)
      aTCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGAGTGGATCGAAACATCTCGGTGCCGTGGCTCG.T.CCAGCCg                                                                                                 >  2:2519687/1‑101 (MQ=255)
        cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.c                                                                                              >  2:3581973/1‑101 (MQ=255)
          aaaCATCGCCGCTTTGGCGGTGGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                                            >  1:2546586/1‑101 (MQ=255)
          aaaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCt                                                                                            >  2:1981166/1‑101 (MQ=255)
              aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtc                                                                                        <  1:2519588/101‑1 (MQ=255)
               tCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcg                                                                                       >  1:160646/1‑101 (MQ=255)
                 gccgcTTTGGCGGTCGACAGGCTGGTAACGTGACGCTCCCCCCGCGGGGGAGTGGATCGAAACATCTCGGTGCCGCCGCTCG.T.CCAGCCGG.CCGCCtcgtc                                                                                     >  1:2790155/1‑101 (MQ=38)
                  ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcgtcg                                                                                    <  2:4337967/101‑1 (MQ=255)
                  ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCtcgtcg                                                                                    <  2:2726385/101‑1 (MQ=255)
                   cgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCACGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGc                                                                                   >  2:2981382/1‑101 (MQ=255)
                     cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTc                                                                                 >  1:84126/1‑101 (MQ=255)
                     cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTc                                                                                 >  2:2578642/1‑101 (MQ=255)
                          gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCGCGTCGCTcccccc                                                                            >  2:1415436/1‑101 (MQ=255)
                           cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTccccccc                                                                           >  1:308252/1‑100 (MQ=255)
                            ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCcgc                                                                          >  2:2642920/1‑101 (MQ=255)
                            ggTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCcgc                                                                          >  1:3022552/1‑101 (MQ=255)
                                gACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCgggg                                                                      >  2:1000043/1‑101 (MQ=255)
                                 aCAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCggggg                                                                     <  1:715024/101‑1 (MQ=255)
                                  cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                                    <  1:1921584/101‑1 (MQ=255)
                                  cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGc                                                                    <  2:1855227/101‑1 (MQ=255)
                                  cAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCCGCCCGTCGCTCCCCCCCCGGGGGc                                                                    >  1:282830/1‑101 (MQ=38)
                                     nnnnnnnaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGtn                                                                 <  1:1870365/94‑2 (MQ=255)
                                     gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCATCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTg                                                                 >  2:1051501/1‑101 (MQ=255)
                                     gCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCGGGCGG.CCTGCTCGGCGCTCCCCCCGCTGGGGCGTg                                                                 >  2:1762548/1‑101 (MQ=38)
                                      agcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.TGGCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTgg                                                                <  2:2096070/91‑1 (MQ=2)
                                      aaggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTgg                                                                >  1:1571544/11‑101 (MQ=2)
                                       gcggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  1:3181707/92‑1 (MQ=0)
                                       aggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  1:2635088/92‑1 (MQ=0)
                                        ggccggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  1:1704250/93‑1 (MQ=2)
                                         ggctgcggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGGGGATc                                                             >  1:3221567/8‑101 (MQ=0)
                                         ctcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             >  2:4065638/8‑101 (MQ=2)
                                          tcgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            >  1:2408261/7‑101 (MQ=2)
                                          gctgcgggcGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  1:3283593/93‑1 (MQ=2)
                                          ccgcacgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  2:828281/95‑1 (MQ=2)
                                           cggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           <  1:916137/96‑1 (MQ=2)
                                           aaCGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGa                                                           >  1:428770/1‑101 (MQ=255)
                                            ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          >  1:3928751/5‑101 (MQ=2)
                                            ggtggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          >  1:2108022/5‑101 (MQ=2)
                                             gtggtCGCCCCCCCCGCGGGGGCGCGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         <  1:3480137/98‑1 (MQ=2)
                                             ccggtCGCGCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCGGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         <  2:3042668/98‑1 (MQ=2)
                                             cGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         >  2:4106974/1‑101 (MQ=255)
                                              ctgccccTCCCCCCGCGGGGGCGTGGATCCAAACACCTCGGTGCCGCCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                        <  2:4212068/95‑1 (MQ=37)
                                               tgtCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                       <  1:1130060/101‑1 (MQ=255)
                                               ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacc                                                       <  2:2144519/100‑1 (MQ=2)
                                               ggtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAact                                                       <  1:1300839/100‑2 (MQ=2)
                                               cgtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaat                                                       >  2:70497/2‑99 (MQ=2)
                                                gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCCCGGGGGCGTGGATCGCAactg                                                      >  2:1610703/1‑99 (MQ=2)
                                                gtCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacta                                                      <  2:3646860/101‑3 (MQ=2)
                                                 gcGCCCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgc                                                     <  2:1132644/100‑4 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccacc                                                    >  1:4141306/1‑101 (MQ=255)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatg                                                    <  2:3549457/101‑5 (MQ=2)
                                                   gccccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                                   <  1:3151513/98‑5 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                                   >  1:4033531/1‑96 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                                   <  1:2585977/101‑6 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgt                                                   >  1:2376134/1‑96 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                                   >  1:2968658/1‑97 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgc                                                   <  2:1502136/101‑5 (MQ=2)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCtt                                                  >  2:2359555/1‑101 (MQ=255)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgct                                                  <  1:1370187/101‑7 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                 <  2:1206696/101‑1 (MQ=255)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                 >  1:245244/1‑101 (MQ=255)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctg                                                 <  2:1273044/101‑8 (MQ=2)
                                                     cccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTg                                                 <  1:3422241/100‑1 (MQ=255)
                                                      ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGc                                                >  1:4202298/1‑101 (MQ=255)
                                                      ccccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCACCGCGGGGGCGTGGATCGAAACCACCTTGc                                                <  1:1030052/101‑1 (MQ=255)
                                                      ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                                >  1:3664895/1‑94 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACGCG.GCAACCGG.CCCG.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccccgcggc                                                >  1:3492231/1‑94 (MQ=2)
                                                       cccccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGC.CTAT.G.GCTGACGGACCCGCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactgcgtgcgc                                               >  2:3099463/1‑92 (MQ=2)
                                                       cccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAactatgctggc                                               >  2:2200738/1‑92 (MQ=2)
                                                        ccccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCTCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCgg                                              >  2:482597/1‑101 (MQ=255)
                                                        ccccGCGGGGGCGTGGATCGAAACA.CCCGGCGCCGCAGCGAG.T.GCGTCTGGAACAGGATGTCGCTCCCCCCGCGGGGGCGGGGATCGAAactatactggct                                              >  1:1853914/1‑91 (MQ=2)
                                                         cccGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGa                                             >  1:2745835/1‑101 (MQ=255)
                                                               ggggCGTGGATCGAACCATCTTGGCGCCGTTGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCGTGCGGATTGTCa                                       <  2:2432641/101‑1 (MQ=37)
                                                               ggggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCa                                       >  2:3388108/1‑101 (MQ=255)
                                                                gggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCAc                                      >  1:4078246/1‑101 (MQ=255)
                                                                 ggCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACt                                     >  1:1667766/1‑101 (MQ=255)
                                                                    gTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTc                                  >  2:2672026/1‑101 (MQ=255)
                                                                     tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                 <  2:385895/101‑1 (MQ=255)
                                                                     tGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTcg                                 <  1:1068494/101‑1 (MQ=255)
                                                                         tCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCACCTTGCGGATTGTCACTTTCGCggg                             >  1:490881/1‑101 (MQ=255)
                                                                         tCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCCCTTTCGCggg                             >  1:1531161/1‑101 (MQ=255)
                                                                           gAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGc                           >  2:203211/1‑101 (MQ=255)
                                                                            aaaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                          <  2:514822/101‑1 (MQ=255)
                                                                            aaaCATCCCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCg                          <  2:2882355/101‑1 (MQ=255)
                                                                             aaCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGa                         <  2:3835187/101‑1 (MQ=255)
                                                                              aCATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGG.TCGAAAACCCCCTTCGGGTTGGCCCTTTTGCCGGGCGAAg                       >  2:1674191/1‑101 (MQ=25)
                                                                               cctctcGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                       <  2:3182117/99‑1 (MQ=255)
                                                                               cATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAGCACCTTGCGGATTGTCACTTTCGCGGGGCGAAg                       >  1:3789093/1‑101 (MQ=255)
                                                                                aTCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                      >  2:1325554/1‑101 (MQ=255)
                                                                                aTCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGc                      <  1:2219277/101‑1 (MQ=255)
                                                                                 tctcGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCg                     >  1:2702784/1‑101 (MQ=255)
                                                                                     ggTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGc                 <  1:4443810/101‑1 (MQ=255)
                                                                                        gCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCCcc              >  1:4317755/1‑101 (MQ=255)
                                                                                         ccGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTccc             <  1:1981295/101‑1 (MQ=255)
                                                                                                  g.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCgggg    >  2:3989161/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
GCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCG.T.CCAGCCGG.CCTGCTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCACCTTGCGGATTGTCACTTTCGCGGGGCGAAGCGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1395452‑1395652

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG