Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,154 G→A 4.5% A12A (GCG→GTA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1540G→A 4.5% 11.3 ‑0.8 ‑1.5156A12A (GCG→GCARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (71/77):  total (74/82)
Fisher's exact test strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.27e-02

Only 100 of 212 total aligned reads displayed.

GGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1396054‑1396254
                                                                                                      |                                                                                                     
ggATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTcgcg                                                                                                       >  1:2532987/1‑101 (MQ=255)
  aTCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgcc                                                                                                     <  1:701668/101‑1 (MQ=255)
   tCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccg                                                                                                    <  2:3994801/101‑1 (MQ=255)
   tCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccg                                                                                                    <  2:370312/101‑1 (MQ=255)
    cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                   <  1:3882472/101‑1 (MQ=255)
       aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                                <  2:1421925/101‑1 (MQ=255)
       aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                                >  2:554489/1‑101 (MQ=255)
       aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                                <  2:2296904/101‑1 (MQ=255)
       aaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCAc                                                                                                <  2:1324920/101‑1 (MQ=255)
        aCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACg                                                                                               <  1:4066658/101‑1 (MQ=255)
           ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcccacccg                                                                                            >  1:3099263/1‑95 (MQ=255)
           ggCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTg                                                                                           <  2:1193340/101‑1 (MQ=255)
            gcgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGc                                                                                          <  1:3417775/101‑1 (MQ=255)
              gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCgg                                                                                        >  1:3500820/1‑100 (MQ=255)
              gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGt                                                                                        >  2:1760031/1‑101 (MQ=255)
               cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTc                                                                                       <  1:3920597/101‑1 (MQ=255)
               cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCCCCACG.CTGCGTc                                                                                       >  1:938107/1‑101 (MQ=255)
                aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCg                                                                                      >  2:2475520/1‑101 (MQ=255)
                aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCg                                                                                      >  2:4092765/1‑101 (MQ=255)
                 tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CCGCGTCGc                                                                                     >  1:1022697/1‑101 (MQ=255)
                 tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGc                                                                                     >  2:3630665/1‑101 (MQ=255)
                  cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCt                                                                                    <  2:1055562/101‑1 (MQ=255)
                   tGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCGGCGCTc                                                                                   >  2:3019500/1‑101 (MQ=255)
                     aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCGTCGCTccc                                                                                 >  2:4107885/1‑101 (MQ=255)
                     aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTccc                                                                                 <  2:3901952/101‑1 (MQ=255)
                     aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTccc                                                                                 >  1:3549689/1‑101 (MQ=255)
                      tGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGGCACG.CTGCGTCGCTcccc                                                                                >  2:470058/1‑101 (MQ=255)
                      tGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTcccc                                                                                <  2:507125/101‑1 (MQ=255)
                        tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCGTCGCCcccccc                                                                              >  2:3084293/1‑101 (MQ=255)
                          tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcgc                                                                            >  2:4191456/1‑101 (MQ=255)
                          tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcgc                                                                            >  1:562775/1‑101 (MQ=255)
                           atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCC.CCGCGGCCCCCCCCCcccg                                                                           >  1:892226/1‑101 (MQ=25)
                           atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCcgcg                                                                           <  1:3704234/101‑1 (MQ=255)
                           atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCCCCCCCcgcg                                                                           >  1:3191280/1‑101 (MQ=255)
                             gCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCggg                                                                         >  1:647074/1‑101 (MQ=255)
                             gCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCggg                                                                         <  1:1156947/101‑1 (MQ=255)
                              cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCgggg                                                                        <  2:1655156/101‑1 (MQ=255)
                              cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCgggg                                                                        >  2:3591668/1‑101 (MQ=255)
                              cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCgggg                                                                        <  1:1215641/101‑1 (MQ=255)
                              cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CCGCGGCGCTCCCCCCGCgggg                                                                        >  2:925479/1‑101 (MQ=255)
                               ccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCggggg                                                                       >  1:2857460/1‑101 (MQ=255)
                               ccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGGCGCTCCCCCCGCggggg                                                                       >  1:3965988/1‑101 (MQ=255)
                                cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCgggggg                                                                      >  2:3887366/1‑100 (MQ=255)
                                   tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                   >  1:1586227/11‑101 (MQ=9)
                                   tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                   <  2:3121347/91‑1 (MQ=9)
                                    gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  1:616963/92‑1 (MQ=2)
                                    gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCCGGGGCGGgg                                                                  >  2:1499785/1‑101 (MQ=255)
                                     agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTggg                                                                 <  2:1590442/101‑2 (MQ=255)
                                       aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               >  2:2697953/1‑101 (MQ=255)
                                         ggCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCCCGGGGGCGTGGATCGa                                                             >  1:845441/1‑101 (MQ=255)
                                           cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGAttcaaa                                                           >  1:3280479/1‑96 (MQ=255)
                                           cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           >  1:4268349/1‑101 (MQ=255)
                                           cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  1:537105/101‑1 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGGGGGGATCGCAAcc                                                         >  1:4202618/1‑101 (MQ=255)
                                             gTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTTTTCGCC.AACTCG.CAGGCCCGCCCCCCCACGCCTGC.CCTCTCCCTCCGGGGGGCGGTGGATGGGAAcc                                                         >  2:2366674/1‑101 (MQ=9)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGc                                                     <  1:190951/101‑1 (MQ=255)
                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGc                                                     <  2:821189/101‑1 (MQ=255)
                                                   ccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGa                                                   >  1:2192144/1‑101 (MQ=255)
                                                    cccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaa                                                  <  1:1004755/101‑1 (MQ=255)
                                                     ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaa                                                 >  2:4298928/1‑101 (MQ=255)
                                                        cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                                              <  2:3358831/101‑1 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                                            >  1:28233/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGc                                           >  1:1987193/1‑101 (MQ=255)
                                                            ggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGcc                                          >  1:1380032/1‑101 (MQ=255)
                                                             gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCa                                         <  2:3483793/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                        <  2:2010350/101‑1 (MQ=255)
                                                              ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                        <  2:2622726/101‑1 (MQ=255)
                                                               gCGTGGATCGAGACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                                       <  1:1276218/101‑1 (MQ=255)
                                                                cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGc                                      >  2:3567995/1‑101 (MQ=255)
                                                                 gTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGcc                                     >  1:2469021/1‑101 (MQ=255)
                                                                  tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                                    <  1:2366345/101‑1 (MQ=255)
                                                                  tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                                    <  1:3084517/101‑1 (MQ=255)
                                                                   ggATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTc                                   <  2:1128865/101‑1 (MQ=255)
                                                                    gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGGCATGCCCcc                                  >  2:862384/1‑101 (MQ=255)
                                                                     aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg                                 >  2:1709740/1‑101 (MQ=255)
                                                                      tCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgg                                >  2:1634177/1‑101 (MQ=255)
                                                                      tCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgg                                <  1:2144205/101‑1 (MQ=255)
                                                                       cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCggg                               <  1:470110/101‑1 (MQ=255)
                                                                        gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgggg                              >  1:744698/1‑100 (MQ=255)
                                                                          aaCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGgtgt                            <  2:3908046/101‑1 (MQ=255)
                                                                           aCTTTTCCTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc                           <  2:2179115/101‑1 (MQ=255)
                                                                             ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                         <  1:985873/101‑1 (MQ=255)
                                                                             ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                         <  1:1537340/101‑1 (MQ=255)
                                                                             ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCCGGGGTCTg                         >  2:3201355/1‑101 (MQ=255)
                                                                             atttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                         <  2:2794350/100‑1 (MQ=255)
                                                                              tttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTgg                        <  1:1643388/101‑1 (MQ=255)
                                                                                 cGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGcgg                     <  2:2884333/101‑3 (MQ=255)
                                                                                 cGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTg                     >  2:156754/1‑101 (MQ=255)
                                                                                   tC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAAGGCCATGCCTCCGGGTGTCTGGCTGTc                   >  1:782812/1‑101 (MQ=255)
                                                                                      aTCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGc                 >  2:2496218/1‑101 (MQ=255)
                                                                                       tCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGGCTGGCTGTCGCt                >  1:2968694/1‑101 (MQ=255)
                                                                                           g.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGCAACCCGGCGAAAAACGGCCCTGCCTCCGGGTGTCTGGCTGTCGCTcccc            >  2:2828470/1‑101 (MQ=255)
                                                                                           g.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccc            <  2:2857252/101‑1 (MQ=255)
                                                                                           g.CAGGCTCGCGCCGCCACC.CTGCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCCGGCGAAAAACAGCCATGCCTCCCGGTGTCTGGCTGTCCCTcccc            >  1:1964771/1‑101 (MQ=37)
                                                                                              aGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc          >  2:1943024/1‑101 (MQ=255)
                                                                                              aGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc          >  1:3435522/1‑101 (MQ=255)
                                                                                                    gcgcCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCgggg    <  2:2192005/101‑1 (MQ=255)
                                                                                                     cgccgcCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggggg   >  2:195935/1‑101 (MQ=255)
                                                                                                     cgccgcCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggggg   >  1:2582251/1‑101 (MQ=255)
                                                                                                      gccgccACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc  <  2:91696/101‑1 (MQ=255)
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GGATCGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1396054‑1396254

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG