Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,478 A→C 5.6% E120D (GAA→GAC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,4780A→C 5.6% 20.7 ‑2.1 ‑0.0197E120D (GAA→GACRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/3):  ref base (87/99):  total (95/102)
Fisher's exact test strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.53e-01

Only 100 of 212 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCT  >  NC_014034/1396379‑1396578
                                                                                                   |                                                                                                     
cccGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGaaa                                                                                                      <  1:346199/101‑1 (MQ=255)
cccGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGaaa                                                                                                      <  2:345033/101‑1 (MQ=255)
cccGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGaaa                                                                                                      <  1:187107/101‑1 (MQ=255)
  cgcgGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAAtt                                                                                                    <  2:4191064/101‑1 (MQ=255)
    ggggggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTcc                                                                                                  <  2:818890/100‑1 (MQ=255)
    cgGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTcc                                                                                                  <  1:1948230/101‑1 (MQ=255)
     gggggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCcg                                                                                                 <  1:4363096/101‑1 (MQ=255)
     gggggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCcg                                                                                                 >  1:206977/1‑101 (MQ=255)
      ggggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCcgc                                                                                                <  2:2104686/101‑1 (MQ=255)
      ggggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCcgc                                                                                                <  1:449237/101‑1 (MQ=255)
        ggCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCgg                                                                                              <  1:2797402/101‑1 (MQ=255)
           gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGaa                                                                                           <  2:2582080/101‑1 (MQ=255)
           gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGaa                                                                                           >  1:2553633/1‑101 (MQ=255)
               aTCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAAcgc                                                                                       >  1:2512934/1‑101 (MQ=255)
                  gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggc                                                                                    >  2:1559833/1‑101 (MQ=255)
                  gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggc                                                                                    <  2:1958735/101‑1 (MQ=255)
                  gAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGCCGCTACCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggc                                                                                    >  1:1249967/1‑101 (MQ=255)
                   aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggcg                                                                                   >  1:3596670/1‑101 (MQ=255)
                   aaaCCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggcg                                                                                   >  1:215409/1‑101 (MQ=255)
                      ccGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggcggcg                                                                                >  2:3302961/1‑101 (MQ=255)
                       cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGa                                                                               <  2:3629792/101‑1 (MQ=255)
                        gcgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAg                                                                              <  2:1693431/101‑1 (MQ=255)
                         cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                             >  2:1539486/1‑101 (MQ=255)
                          gcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcggcgggcagtc                                                                            <  1:98669/101‑7 (MQ=255)
                          gcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCa                                                                            <  2:3635654/101‑1 (MQ=255)
                           cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                           <  1:295585/101‑1 (MQ=255)
                           cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                           <  1:2262518/101‑1 (MQ=255)
                            ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                         >  1:3239671/1‑101 (MQ=255)
                            ccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.g                                                                         <  1:2428015/101‑1 (MQ=255)
                             cTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.Gt                                                                        >  1:3273411/1‑101 (MQ=255)
                               gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                      >  2:3940587/1‑101 (MQ=255)
                               gCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCg                                                                      <  1:4290709/101‑1 (MQ=255)
                                cGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAG.GTCGc                                                                     >  2:3117329/1‑101 (MQ=255)
                                   gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                  >  2:2025512/1‑101 (MQ=255)
                                    tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                 >  1:835709/1‑101 (MQ=255)
                                    tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                 >  2:4268755/1‑101 (MQ=255)
                                    tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GGCGCTccc                                                                 >  1:1806124/1‑101 (MQ=255)
                                     gATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccc                                                                <  1:557946/101‑1 (MQ=255)
                                       tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                              <  1:1654634/101‑1 (MQ=255)
                                       tGGCGACGGGCACCACGGCGCTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                              <  2:2773348/101‑1 (MQ=255)
                                        ggCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccca                                                             >  2:1654528/1‑100 (MQ=255)
                                        ggCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCcg                                                             >  1:4187471/1‑101 (MQ=255)
                                        ggCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCcg                                                             >  2:1173729/1‑101 (MQ=255)
                                           ggcGGGCACCCCGGCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgg                                                          <  1:927802/99‑1 (MQ=255)
                                             cGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgggg                                                        >  1:699924/1‑101 (MQ=255)
                                             cGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgggg                                                        >  2:1744702/1‑101 (MQ=255)
                                              gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                       <  1:494071/101‑1 (MQ=255)
                                              gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                       >  2:3067359/1‑101 (MQ=255)
                                               ggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAGGGACCTGAAATTCCGCGGGGAATGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGc                                                      >  1:3805944/1‑101 (MQ=255)
                                               ggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGc                                                      <  2:3607202/101‑1 (MQ=255)
                                                gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                     >  2:3715976/1‑101 (MQ=255)
                                                gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                     >  2:2671276/1‑101 (MQ=255)
                                                  accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                   >  1:2677001/1‑101 (MQ=255)
                                                  accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                   <  1:4299197/101‑1 (MQ=255)
                                                  accacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTg                                                   >  2:2569929/1‑101 (MQ=255)
                                                   cttccgatctCGCACCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  2:3689453/92‑1 (MQ=38)
                                                   ccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:3246819/101‑1 (MQ=255)
                                                     tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                               >  2:2926361/7‑101 (MQ=2)
                                                     acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  2:84265/101‑1 (MQ=255)
                                                     acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                >  2:3284226/1‑101 (MQ=255)
                                                     acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:38229/101‑1 (MQ=255)
                                                      cggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               >  2:27172/1‑101 (MQ=255)
                                                         cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            <  2:3760315/101‑1 (MQ=255)
                                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           >  2:4406262/1‑101 (MQ=255)
                                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  2:1217914/101‑1 (MQ=255)
                                                          ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           <  2:405988/101‑1 (MQ=255)
                                                            tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgc                                        >  1:132077/1‑99 (MQ=2)
                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGagatcggaag                                        >  1:3689706/1‑92 (MQ=255)
                                                             cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                        <  2:552835/101‑1 (MQ=255)
                                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                       <  2:2285844/101‑1 (MQ=255)
                                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                      <  2:475743/101‑5 (MQ=0)
                                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                      >  1:756548/1‑97 (MQ=0)
                                                              gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                      >  1:1826570/1‑97 (MQ=0)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                                     <  1:2050827/101‑1 (MQ=255)
                                                                tCCCCCCGCGGGGGCGTGGATCGAAACGCTCCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGCGGATCGAAAcaccggc                                    >  1:3751175/1‑95 (MQ=0)
                                                                 ccccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCttt                                    >  2:661692/1‑101 (MQ=255)
                                                                  cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                   <  2:889485/101‑1 (MQ=255)
                                                                  cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                   >  2:3542081/1‑101 (MQ=255)
                                                                    cccGCGGGGGCGTGGATCGAAACAGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCAAAACTGCTTTCGt                                 <  1:4148103/101‑1 (MQ=255)
                                                                     ccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                                <  2:2748458/101‑1 (MQ=255)
                                                                         gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCCC.GTCGTTCCCCCCGCGGGGGCGCGGATCGAAACTGCTTTCGTGGGAc                            >  2:4429082/1‑101 (MQ=255)
                                                                         gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                            >  2:1584674/1‑101 (MQ=255)
                                                                          ggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                           <  1:4026033/101‑1 (MQ=255)
                                                                            ggCGTTGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTgg                         <  2:3558959/101‑1 (MQ=255)
                                                                               gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCa                      >  2:763393/1‑101 (MQ=255)
                                                                                tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                     <  2:707756/101‑1 (MQ=255)
                                                                                tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                     <  2:4337350/101‑1 (MQ=255)
                                                                                 ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                    >  1:664905/1‑101 (MQ=255)
                                                                                  gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                   <  2:2422072/101‑1 (MQ=255)
                                                                                     cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgccgcc                >  1:3589218/1‑101 (MQ=255)
                                                                                      gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc               >  2:3030819/1‑101 (MQ=255)
                                                                                      gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc               <  1:1047790/101‑1 (MQ=255)
                                                                                       aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg              >  1:3392202/1‑101 (MQ=255)
                                                                                       aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccg              <  2:1301747/101‑1 (MQ=255)
                                                                                        aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc             <  2:2877478/101‑1 (MQ=255)
                                                                                        aaCGGACCTGAAATTCCGCGGGAAAAGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgcccgc             >  2:567031/1‑101 (MQ=255)
                                                                                            gACCCGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCa         <  2:1240579/101‑1 (MQ=255)
                                                                                             aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCaa        <  1:3174943/101‑1 (MQ=255)
                                                                                                  aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc   >  1:122189/1‑101 (MQ=255)
                                                                                                   aaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt  >  2:830669/1‑101 (MQ=255)
                                                                                                   |                                                                                                     
CCCGCGGGGGCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCT  >  NC_014034/1396379‑1396578

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG