Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,477 C→T 14.8% T453T (ACC→AAT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4770C→T14.8% 91.6 ‑2.0 ‑0.0236T453T (ACC→ACTRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/19):  ref base (119/81):  total (136/100)
Fisher's exact test strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.94e-01

Only 100 of 259 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCC  >  NC_014034/1397377‑1397577
                                                                                                      |                                                                                                      
cccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acc                                                                                                        <  2:3559294/101‑1 (MQ=255)
 ccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acca                                                                                                       <  2:147597/101‑1 (MQ=255)
  cgcgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.accac                                                                                                      <  1:708068/101‑1 (MQ=255)
   gcgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.g                                                                                                    <  1:3427816/101‑1 (MQ=255)
   gcgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.g                                                                                                    <  1:3518290/101‑1 (MQ=255)
      ggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCaa                                                                                                 <  2:1833453/101‑1 (MQ=255)
       gggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.c                                                                                               <  1:4359660/101‑1 (MQ=255)
          cGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGc                                                                                            >  2:1448018/1‑101 (MQ=255)
          cGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGc                                                                                            >  1:3684800/1‑101 (MQ=255)
             ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCt                                                                                         >  1:4260984/1‑101 (MQ=255)
              gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTg                                                                                        <  2:854780/101‑1 (MQ=255)
                ctgAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GTAA.CCGCCCTGca                                                                                      <  1:2414944/99‑1 (MQ=255)
                 tgAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcac                                                                                     <  2:4227498/100‑1 (MQ=255)
                 cGAAACTCTTCAGTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcac                                                                                     >  1:2427023/1‑101 (MQ=255)
                 cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcac                                                                                     >  2:577065/1‑101 (MQ=255)
                   aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcacca                                                                                   >  1:4324662/1‑101 (MQ=255)
                    aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGcaccac                                                                                  <  2:2409425/101‑1 (MQ=255)
                     aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCaccacc                                                                                 >  2:2820852/1‑101 (MQ=255)
                      ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCg                                                                                >  2:3684923/1‑101 (MQ=255)
                       tctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCGCCgg                                                                               <  2:402389/101‑1 (MQ=255)
                        ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGt                                                                              >  1:793775/1‑101 (MQ=255)
                        ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCACCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGt                                                                              >  2:2279656/1‑101 (MQ=255)
                         ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTc                                                                             >  1:2764568/1‑101 (MQ=255)
                         ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTc                                                                             <  1:874534/101‑1 (MQ=255)
                             aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTc                                                                         >  1:3891165/1‑101 (MQ=255)
                             aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTc                                                                         >  1:760246/1‑101 (MQ=255)
                             aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTc                                                                         <  2:1412342/101‑1 (MQ=255)
                              tGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTcc                                                                        >  1:1761681/1‑101 (MQ=255)
                                  tatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTcccccc                                                                    <  1:1262614/101‑1 (MQ=255)
                                   atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                   <  2:951813/101‑1 (MQ=255)
                                   atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                   <  1:2120015/101‑1 (MQ=255)
                                   atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcg                                                                   >  1:1602501/1‑101 (MQ=255)
                                     ggCACAACCGTCCGCGACATGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcgcg                                                                 <  2:2001456/101‑1 (MQ=255)
                                     ggCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCcgcg                                                                 >  1:4238246/1‑101 (MQ=255)
                                       cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggg                                                               >  2:3922258/1‑101 (MQ=255)
                                       cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggg                                                               >  2:135557/1‑101 (MQ=255)
                                       cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggg                                                               >  2:3581633/1‑101 (MQ=255)
                                        aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACAAC.GCAA.CCGCCCTGCACCAACGGTCGCTCCCCCCGCgggg                                                              >  2:2570081/1‑101 (MQ=255)
                                           aCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCg                                                           >  1:4142256/1‑101 (MQ=255)
                                           aCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.ACAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCCGGGGCg                                                           >  2:2693687/1‑101 (MQ=255)
                                            ccGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGt                                                          <  2:4324393/101‑1 (MQ=255)
                                            ccGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGt                                                          <  1:1984003/101‑1 (MQ=255)
                                             cGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTg                                                         <  2:173220/101‑1 (MQ=255)
                                              gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  2:3228578/101‑1 (MQ=255)
                                              gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTgg                                                        >  1:4075263/1‑101 (MQ=255)
                                              gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGACCTGCACCACCGGTCGATCCCCCCGCGGGGGAGTgg                                                        >  2:2545645/1‑101 (MQ=255)
                                                 cgcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     >  1:205645/1‑101 (MQ=255)
                                                 cgcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  1:599647/101‑1 (MQ=255)
                                                  gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  1:2239037/1‑101 (MQ=255)
                                                    gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  >  2:1102270/1‑101 (MQ=255)
                                                    accgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  1:965237/6‑101 (MQ=2)
                                                       ctGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCCCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaaca                                               <  2:3027066/100‑1 (MQ=255)
                                                       aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaaca                                               >  1:578383/1‑101 (MQ=255)
                                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaac                                             <  1:2550768/101‑1 (MQ=255)
                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacgg                                              >  2:3178708/1‑99 (MQ=2)
                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAccc                                           <  2:3316427/101‑1 (MQ=255)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtg                                            <  1:1717244/101‑5 (MQ=2)
                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgt                                           <  1:1672519/101‑6 (MQ=2)
                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggggt                                           >  1:1884655/1‑96 (MQ=2)
                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggcgt                                           >  2:1969851/1‑96 (MQ=2)
                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGa                                         >  1:548051/1‑101 (MQ=255)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                          <  2:181173/101‑7 (MQ=2)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                          <  2:308167/101‑7 (MQ=2)
                                                                cccccGCGGGGGCGTGGATCGAAACATCGATTATC.AAATCGGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                        <  1:4436900/101‑9 (MQ=2)
                                                                cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                        <  1:3026767/101‑9 (MQ=2)
                                                                 ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                                       <  2:2083408/101‑10 (MQ=2)
                                                                 ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                     <  1:1994561/101‑1 (MQ=255)
                                                                  cccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCC.GCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccagc                                      <  1:1909549/101‑11 (MQ=2)
                                                                  cccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                    >  1:4280316/1‑101 (MQ=255)
                                                                   ccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                   >  1:2391337/1‑101 (MQ=255)
                                                                   ccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATgg                                   <  1:3766829/101‑1 (MQ=255)
                                                                     gcgGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                                 >  1:3400422/1‑101 (MQ=255)
                                                                       gggggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                               >  2:4089654/1‑101 (MQ=255)
                                                                       gggggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                               >  2:1272215/1‑101 (MQ=255)
                                                                         gggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                             <  2:2426866/101‑1 (MQ=255)
                                                                         gggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                             >  2:1144214/1‑101 (MQ=255)
                                                                          ggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                            >  1:2700489/1‑101 (MQ=255)
                                                                          ggCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                            <  2:1602395/101‑1 (MQ=255)
                                                                           gCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                           >  2:1900110/1‑101 (MQ=255)
                                                                            cGTGGATCGAAACACCG.TCATC.ACCAC.GCAC.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATAATGGCCCGCCTCa                          <  2:2894483/101‑1 (MQ=255)
                                                                            cGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                          <  1:3659040/101‑1 (MQ=255)
                                                                             gTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                         >  1:3284405/1‑101 (MQ=255)
                                                                                gATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACAACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                      >  1:1489945/1‑101 (MQ=255)
                                                                                 aTCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgcc                     >  1:1416367/1‑101 (MQ=255)
                                                                                  tCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccg                    <  1:3112800/101‑1 (MQ=255)
                                                                                   cGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                   <  1:1099134/101‑1 (MQ=255)
                                                                                   cGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                   >  2:297089/1‑101 (MQ=255)
                                                                                    gAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCcgcg                  >  1:2818027/1‑101 (MQ=255)
                                                                                    gAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCcgcg                  >  1:2937446/1‑101 (MQ=255)
                                                                                    gAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCcgcg                  >  1:2193962/1‑101 (MQ=255)
                                                                                      aaCACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCt                >  2:666550/1‑101 (MQ=255)
                                                                                        cacCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGa              <  1:3182106/101‑1 (MQ=255)
                                                                                         acCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGAc             >  2:2120712/1‑101 (MQ=255)
                                                                                           cG.TCTTC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCTGGGGCGTGGATCGAAACAACCCGACCATGGCCCGCCTCAGGGCCGCGCTGACga           >  2:712878/1‑100 (MQ=38)
                                                                                            g.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc          <  2:3470332/101‑1 (MQ=255)
                                                                                                atc.aCCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCt       >  1:1196816/1‑101 (MQ=255)
                                                                                                 tc.aCCAC.GCAA.CCGCCGTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTc      <  2:3257767/101‑1 (MQ=255)
                                                                                                  c.accac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc     <  1:1001709/101‑1 (MQ=255)
                                                                                                  c.accac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc     >  2:519135/1‑101 (MQ=255)
                                                                                                      cac.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc  <  1:1438461/101‑1 (MQ=255)
                                                                                                      |                                                                                                      
CCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCAC.GCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCC  >  NC_014034/1397377‑1397577

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG