Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,385 T→G 16.1% intergenic (‑164/‑794) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3850T→G16.1% 108.8 0.0 ‑0.1262intergenic (‑164/‑794)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (20/23):  ref base (99/120):  total (119/143)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.22e-01

Only 100 of 282 total aligned reads displayed.

GGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGC  >  NC_014034/1434285‑1434485
                                                                                                      |                                                                                                      
ggTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..Gtct                                                                                                        <  1:497098/101‑1 (MQ=255)
 gTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..Gtctc                                                                                                       <  1:85483/101‑1 (MQ=255)
  tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                      <  2:1988112/101‑1 (MQ=255)
  tataTGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                      <  1:1864806/101‑1 (MQ=255)
  tataAGCACCGCGGGCCGTAGATCGAAACAGGCGGGTTTGGGCGGAACCCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTcc                                                                                                      <  2:2150501/101‑1 (MQ=25)
    tatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGc                                                                                                    >  2:1630598/1‑101 (MQ=255)
    tatGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGc                                                                                                    >  2:1063157/1‑101 (MQ=255)
     atGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGct                                                                                                   <  2:1733309/101‑1 (MQ=255)
       gCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGctct                                                                                                 <  2:2459098/101‑1 (MQ=255)
        cACTGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAC..GTCTCCGCTCTg                                                                                                >  2:1167739/1‑101 (MQ=255)
         aCCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGc                                                                                               >  2:195163/1‑101 (MQ=255)
           cGCTTACCGTGGATCGAAACACGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCtt                                                                                             <  2:33527/101‑1 (MQ=255)
            gCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTc                                                                                            <  2:2877209/101‑1 (MQ=255)
             cTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCa                                                                                           <  2:2395705/101‑1 (MQ=255)
              ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCAc                                                                                          <  2:3908332/101‑1 (MQ=255)
              ttACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCAc                                                                                          >  2:719398/1‑101 (MQ=255)
               tACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg                                                                                         >  2:3943652/1‑101 (MQ=255)
                aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                       <  2:4388734/101‑1 (MQ=255)
                aCCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACg.g                                                                                       >  2:945568/1‑101 (MQ=255)
                  cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.Ct                                                                                    >  1:3832469/1‑101 (MQ=255)
                  cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.Ct                                                                                    >  1:4335384/1‑101 (MQ=255)
                   gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTg                                                                                   >  2:1348755/1‑101 (MQ=255)
                      gATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGt                                                                                >  1:4382445/1‑101 (MQ=255)
                        tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCa                                                                              <  2:2685201/101‑1 (MQ=255)
                        tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCa                                                                              <  1:2043447/101‑1 (MQ=255)
                          gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAAc                                                                            >  1:4167736/1‑101 (MQ=255)
                            aaCAGGCTGCTTTGGGCGGAACACCTCATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCt                                                                          <  1:1783265/101‑1 (MQ=255)
                                ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTc                                                                      <  2:3124079/101‑1 (MQ=255)
                                 gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCg                                                                     >  2:58299/1‑101 (MQ=255)
                                   tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCt                                                                   <  1:3170026/101‑1 (MQ=255)
                                   tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCt                                                                   >  1:2935972/1‑101 (MQ=255)
                                      tttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGGCAACCTTGTCGCTccc                                                                >  1:163115/1‑101 (MQ=255)
                                        tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTccccc                                                              <  2:2329403/101‑1 (MQ=255)
                                          ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCcg                                                            <  2:1017068/101‑1 (MQ=255)
                                            cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCcgcg                                                          >  2:2971574/1‑101 (MQ=255)
                                             ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCgg                                                         >  2:2538669/1‑101 (MQ=255)
                                             ggAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCgg                                                         >  2:3214101/1‑101 (MQ=255)
                                              gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCCTGTCCCTCCCCCCCCggg                                                        >  2:1936292/1‑101 (MQ=255)
                                                  acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCg                                                    >  2:836379/1‑101 (MQ=255)
                                                    cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                  >  2:1876360/1‑101 (MQ=255)
                                                     tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTgg                                                 <  2:1288748/101‑1 (MQ=255)
                                                       gcgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTg                                                  <  1:2431405/91‑1 (MQ=2)
                                                       aTCCTGCTGGGTCGCTCCCCCGGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                               >  1:2543086/1‑101 (MQ=255)
                                                       aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                               <  1:566512/101‑1 (MQ=255)
                                                       aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGAt                                               <  1:3056009/101‑1 (MQ=255)
                                                        tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  1:4070240/101‑1 (MQ=255)
                                                        tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  1:676489/101‑1 (MQ=255)
                                                        tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                              <  1:2251377/101‑1 (MQ=255)
                                                         gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                >  1:3412312/9‑101 (MQ=2)
                                                         gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                >  2:4286213/9‑101 (MQ=2)
                                                         gggctatcgTCGCCCCGCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                <  2:3494524/93‑1 (MQ=2)
                                                          ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                               <  1:3548482/94‑1 (MQ=2)
                                                           tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           <  2:2122263/101‑1 (MQ=255)
                                                            ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                             <  1:2200877/96‑1 (MQ=2)
                                                             tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                            >  1:1451945/5‑101 (MQ=2)
                                                              tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                        <  1:3214330/101‑1 (MQ=255)
                                                              atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                           >  2:92064/4‑101 (MQ=2)
                                                               gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                       >  1:3984401/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                        >  2:3512317/1‑100 (MQ=2)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACgg                                     <  1:928347/101‑1 (MQ=255)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgc                                       >  1:3909243/1‑99 (MQ=2)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGa                                    <  1:1685690/101‑1 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                   <  1:694375/101‑1 (MQ=255)
                                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                     <  1:2619103/101‑5 (MQ=2)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                    <  1:2454522/101‑6 (MQ=2)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctca                                    >  2:1420907/1‑96 (MQ=2)
                                                                      tCCCCCCGCGGGGGCGTGGATCGAAACGGGCCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGCCGCTCCCCCCGCGGGGGCGTGGATCGAAacgatgct                                   >  2:3712838/1‑95 (MQ=2)
                                                                       ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                               <  2:1808225/101‑1 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                              <  2:4002554/101‑1 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                              >  1:2911328/1‑101 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                              <  1:2610500/101‑1 (MQ=255)
                                                                        cccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                              >  1:4145621/1‑101 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCG.CTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                                <  2:3796003/101‑10 (MQ=2)
                                                                         ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                             <  1:763554/101‑1 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGCCGCTCCCCCCGCGTGGGGGCGGTTCTAAAAACGGAGTGCCTg                             >  2:3566317/1‑101 (MQ=37)
                                                                          cccGGGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                            <  2:527727/101‑1 (MQ=255)
                                                                          cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                            <  1:1968487/101‑1 (MQ=255)
                                                                           ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                           >  2:2927204/1‑101 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                         >  2:3271822/1‑101 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                         >  1:112119/1‑101 (MQ=255)
                                                                               gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                       >  2:4345552/1‑101 (MQ=255)
                                                                                 gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                     >  1:830453/1‑101 (MQ=255)
                                                                                    cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                  >  1:1287111/1‑101 (MQ=255)
                                                                                    cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                  <  1:1063245/101‑1 (MQ=255)
                                                                                    cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                  >  1:2171886/1‑101 (MQ=255)
                                                                                    cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                  <  1:2729583/101‑1 (MQ=255)
                                                                                      tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                <  2:1548467/101‑1 (MQ=255)
                                                                                       ggATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg               >  2:3045302/1‑101 (MQ=255)
                                                                                           cGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt           >  2:677295/1‑101 (MQ=255)
                                                                                           cGAAAC..CGCCCCGGTCTTGTTCCCG.GCCTGCGCCACCCTTGT.GCCCCCCCCCGGGGGGGGGGGGTCGAAACCCGGATTGCATGGCGCCGAGGGACAGGCtt           <  1:1929593/101‑1 (MQ=2)
                                                                                             aaaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg         >  1:102042/1‑101 (MQ=255)
                                                                                              aaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc        >  2:4228079/1‑101 (MQ=255)
                                                                                              aaC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc        <  2:4083459/101‑1 (MQ=255)
                                                                                                   gTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt     <  2:3298033/101‑1 (MQ=255)
                                                                                                   gTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt     >  1:3355969/1‑101 (MQ=255)
                                                                                                    tctcCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc    >  1:3260301/1‑101 (MQ=255)
                                                                                                    tctcCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGCATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc    >  1:3304569/1‑101 (MQ=255)
                                                                                                     ctcCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTcc   >  1:4301399/1‑100 (MQ=255)
                                                                                                     ctcCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg   >  1:4018680/1‑101 (MQ=255)
                                                                                                      tcCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGc  >  1:1628774/1‑101 (MQ=255)
                                                                                                      |                                                                                                      
GGTATATGCACCGCTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.G.CTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGC  >  NC_014034/1434285‑1434485

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG