Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,459 A→C 28.1% V8V (GTA→GTC RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4590A→C28.1% 183.8 ‑1.5 ‑0.2217V8V (GTA→GTCRCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (27/34):  ref base (84/72):  total (111/106)
Fisher's exact test strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.16e-01

Only 100 of 237 total aligned reads displayed.

CGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGC  >  NC_014034/2155359‑2155559
                                                                                                    |                                                                                                    
cccccTCCCCTTCTGATCCCCTCGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTa                                                                                                      <  1:346486/99‑1 (MQ=255)
  cccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                                    >  2:2485463/1‑101 (MQ=255)
  cccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGAGGCAAGGGGCCTCGTaga                                                                                                    >  2:791631/1‑101 (MQ=255)
   ccTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTagag                                                                                                   >  1:3207261/1‑101 (MQ=255)
     tCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGACGCAAGGGGCCTCGGAGAGCt                                                                                                 >  2:1606494/1‑101 (MQ=255)
      cACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtc                                                                                                >  2:2150208/1‑101 (MQ=255)
       aCCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTGGTAGAGCtct                                                                                               >  1:3122024/1‑100 (MQ=255)
         cTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                                             >  1:416043/1‑101 (MQ=255)
          ttCTGAGCCCCTGGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtcg                                                                                            <  1:2821915/101‑1 (MQ=255)
           tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                                           >  1:4437611/1‑101 (MQ=255)
              gATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGc                                                                                        >  1:2893162/1‑101 (MQ=255)
                     tCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                                 >  1:818782/1‑101 (MQ=255)
                     gcGGGCGCCGCCCCCTCCCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                                 <  2:4284514/100‑1 (MQ=255)
                     ccGGGCGCCGCCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                                 <  2:3030637/100‑1 (MQ=255)
                     ccGGGCCCCGTCCCCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgg                                                                                 <  1:4327806/100‑1 (MQ=255)
                       gggCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTgggg                                                                               >  1:2140914/1‑101 (MQ=255)
                              ccccccgcccccgcccgcGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                                        <  2:850128/91‑1 (MQ=255)
                                ccccgcccccgcccgcCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                                      <  2:50054/93‑1 (MQ=255)
                                caccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                                      >  1:1136534/1‑101 (MQ=255)
                                      ccccccccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                                                <  1:306494/96‑1 (MQ=255)
                                      caccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                                                >  2:1738177/1‑101 (MQ=255)
                                      caccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcaccac                                                                >  1:1273781/1‑101 (MQ=255)
                                         cccccgcgGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                                             <  2:3458913/99‑1 (MQ=255)
                                         cccccgccGGGGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGAt                                                             <  1:1812969/99‑1 (MQ=255)
                                           ccccacgcgcggcAAGCACCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                                           <  2:4014818/93‑1 (MQ=255)
                                            ccgccgGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                                          <  1:606248/101‑1 (MQ=255)
                                             cggggggGGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcg                                                         <  1:802769/97‑1 (MQ=255)
                                               ccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggc                                                       >  1:1500340/1‑101 (MQ=255)
                                                cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                                      >  2:3875971/1‑101 (MQ=255)
                                                cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                                      >  2:407313/1‑101 (MQ=255)
                                                 gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                                     <  2:3280481/101‑1 (MQ=255)
                                                 gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                                     >  2:194524/1‑101 (MQ=255)
                                                 gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                                     >  1:1972937/1‑101 (MQ=255)
                                                    cggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGgcgc                                                  >  2:2230985/1‑101 (MQ=255)
                                                      gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                                                <  2:1549979/99‑1 (MQ=255)
                                                           ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCgg                                           <  2:2735417/101‑1 (MQ=255)
                                                            cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                                          <  1:117665/101‑1 (MQ=255)
                                                            cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                                          >  1:722885/1‑101 (MQ=255)
                                                              cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                                        >  2:4258425/1‑101 (MQ=255)
                                                               gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                                       >  1:78607/1‑101 (MQ=255)
                                                                aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                                      <  2:3002323/101‑1 (MQ=255)
                                                                aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                                      <  1:1236401/101‑1 (MQ=255)
                                                                  aGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCCTCTGGGGCGGGGCCGGCACCACGATGGCGGGGGGGCTCGCCGggcggccg                                    >  2:942364/1‑99 (MQ=255)
                                                                   gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCNCCNNGGCGGCGGCGCTCCCCGGGCGGCCGa                                   >  1:3598218/1‑101 (MQ=255)
                                                                   gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCGGGCGGCTGa                                   >  2:3112701/1‑101 (MQ=255)
                                                                   gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                                   <  1:907775/101‑1 (MQ=255)
                                                                   gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                                   >  2:3589505/1‑101 (MQ=255)
                                                                   ggagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGGGCCTGa                                   >  2:2903888/11‑101 (MQ=255)
                                                                     gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGTGCTCGTCGATGCCATCTGGGGCGGTGCCGTCACAAAGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg                                 >  2:1239165/1‑101 (MQ=255)
                                                                      gaaaacaaTGACCGATGTCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                                <  2:3610242/94‑1 (MQ=255)
                                                                      gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt                                <  2:418279/94‑1 (MQ=255)
                                                                          aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa                            >  2:4357495/4‑101 (MQ=255)
                                                                          aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa                            >  1:1943500/1‑101 (MQ=255)
                                                                            cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAtt                          <  2:3400019/101‑1 (MQ=255)
                                                                            antGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                          >  2:2579376/3‑101 (MQ=255)
                                                                            aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt                          >  1:1633827/2‑101 (MQ=255)
                                                                             aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                         <  2:4022155/101‑1 (MQ=255)
                                                                             aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                         <  1:2784472/101‑1 (MQ=255)
                                                                             aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc                         >  1:2228588/1‑101 (MQ=255)
                                                                             aTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTc                         <  1:2464081/101‑1 (MQ=255)
                                                                              tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg                        <  2:416003/101‑1 (MQ=255)
                                                                              tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg                        <  1:3257311/101‑1 (MQ=255)
                                                                              tGACCGATACCAGAGGCCTCGTCGAGGTCAACGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg                        >  1:3971944/1‑101 (MQ=255)
                                                                               gaccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGa                       <  1:3880653/98‑1 (MQ=255)
                                                                                accGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAc                      <  2:3815147/99‑1 (MQ=255)
                                                                                aGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGAc                      >  1:2005210/1‑101 (MQ=255)
                                                                                 ccGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAcc                     >  1:967410/2‑101 (MQ=255)
                                                                                  cgataccggaggCCTCGTCGAGGTCATCGATGCCCTTTGGGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg                    <  1:3028980/91‑1 (MQ=255)
                                                                                  cGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCg                    <  2:278422/101‑1 (MQ=255)
                                                                                  cGATGCAAGGGGCATCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCCTTCGACCg                    >  2:4382483/1‑101 (MQ=255)
                                                                                  cGATACCAGAGGCCTCGTCTAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg                    <  2:3144636/101‑1 (MQ=255)
                                                                                  cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg                    >  2:809981/1‑101 (MQ=255)
                                                                                  cGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCg                    <  1:1741035/101‑1 (MQ=255)
                                                                                   gataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGa                   >  1:4200882/10‑101 (MQ=255)
                                                                                   gATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGACGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGa                   >  1:1775999/1‑101 (MQ=255)
                                                                                    ataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAg                  >  2:3942549/9‑101 (MQ=255)
                                                                                    ataccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAg                  <  1:255206/93‑1 (MQ=255)
                                                                                    aTGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAg                  <  2:1636543/101‑1 (MQ=255)
                                                                                       ccagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAgggg               >  2:515703/6‑99 (MQ=255)
                                                                                       cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg               <  1:1577283/101‑1 (MQ=255)
                                                                                       cAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTg               >  2:168496/1‑101 (MQ=255)
                                                                                        cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc              >  2:3723932/5‑101 (MQ=255)
                                                                                        cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc              >  1:1618584/5‑101 (MQ=255)
                                                                                        cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc              <  1:3543580/97‑1 (MQ=255)
                                                                                        cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc              <  2:2003137/97‑1 (MQ=255)
                                                                                        cagaggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGc              >  2:908282/5‑101 (MQ=255)
                                                                                        aaGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGAGGCTGATGTGGCATTCCACCGAGGTGc              >  2:2673448/1‑101 (MQ=255)
                                                                                         aGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGGTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCg             >  1:3759839/1‑101 (MQ=255)
                                                                                          ggggCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCgg            >  2:286931/1‑101 (MQ=255)
                                                                                          gaggCCTCGGCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCgg            <  2:541722/99‑1 (MQ=255)
                                                                                           aggCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCggg           >  2:1870436/2‑101 (MQ=255)
                                                                                              gcTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgc        <  2:1008398/100‑1 (MQ=255)
                                                                                              ccTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgcgc        >  2:3235617/1‑101 (MQ=255)
                                                                                               cTCGTCGTGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcg       >  2:1122098/1‑101 (MQ=255)
                                                                                               cTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGgcgcg       >  1:1046059/1‑101 (MQ=255)
                                                                                               cTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgcgcg       <  2:2801308/101‑1 (MQ=255)
                                                                                                tCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGgcgcgc      <  2:1818977/101‑1 (MQ=255)
                                                                                                  gTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCGCCAGGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCaa    <  1:4024891/101‑1 (MQ=255)
                                                                                                  gTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCaa    >  1:4312618/1‑101 (MQ=255)
                                                                                                    agagCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAgc  >  2:2360117/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGC  >  NC_014034/2155359‑2155559

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG