Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,564 C→G 39.9% R43R (CGC→CGG)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,5640C→G39.9% 0.0 ‑1.5 ‑1.2281R43R (CGC→CGG) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (66/46):  ref base (86/83):  total (152/129)
Fisher's exact test strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.96e-02

Only 100 of 312 total aligned reads displayed.

TCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGA  >  NC_014034/2155464‑2155664
                                                                                                    |                                                                                                    
 cgtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCt                                                                                                     >  1:3394810/1‑101 (MQ=255)
 cgtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCt                                                                                                     >  1:1398512/1‑101 (MQ=255)
  gtcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCtt                                                                                                    <  2:1851750/101‑1 (MQ=255)
   tcgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTc                                                                                                   <  2:3554219/101‑1 (MQ=255)
    cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGTGCCCGAGCGCCGCTTcc                                                                                                  >  2:1193245/1‑101 (MQ=255)
    cgATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTcc                                                                                                  >  1:830/1‑101 (MQ=255)
     gATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGAACGAGGTGCGGGCGCGGCGGCGCCGGttttt                                                                                                 >  1:723571/1‑98 (MQ=255)
           aTCTTGGGCGGGGCCGTCACCACGATGGCTTCGTCTCCCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCcg                                                                                           <  2:1775880/101‑1 (MQ=255)
             cTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGCGAGGGGGCGCAAGCGCCGCTTCCTGAGCcgcc                                                                                         >  1:1168163/1‑100 (MQ=255)
                  gcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgctg                                                                                    <  2:818700/101‑1 (MQ=255)
                  gcggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgctg                                                                                    >  2:1107728/1‑101 (MQ=255)
                    ggggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAgctgct                                                                                  >  1:4231188/1‑101 (MQ=255)
                     gggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGctc                                                                                 <  1:2782108/101‑1 (MQ=255)
                      ggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGctct                                                                                <  1:1650005/101‑1 (MQ=255)
                      ggcCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGctct                                                                                <  1:4286676/101‑1 (MQ=255)
                        ccGTCCCCACGATGGCGGCGGCGCTCGGCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTgg                                                                              <  1:3876225/101‑1 (MQ=255)
                         cggccccacGATGGCCGCGGCGCTGGCCGGGCGGCTGATGTGGTATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTggg                                                                             <  2:3692914/96‑1 (MQ=255)
                         cGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTggg                                                                             <  2:3761266/101‑1 (MQ=255)
                          gTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTgggg                                                                            >  2:1225535/1‑100 (MQ=255)
                          gTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGa                                                                            >  2:927635/1‑101 (MQ=255)
                          gTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGa                                                                            <  2:1625672/101‑1 (MQ=255)
                          atCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGa                                                                            >  1:3778892/2‑101 (MQ=255)
                            caccacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGc                                                                          >  2:1485035/1‑101 (MQ=255)
                            caccacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGc                                                                          >  2:3831005/1‑101 (MQ=255)
                            caccacGATGGCGGCGGCGCTAGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGc                                                                          >  1:3297310/1‑101 (MQ=255)
                               gacGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGc                                                                       >  1:524116/2‑101 (MQ=255)
                               cacGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGc                                                                       >  2:3117294/1‑101 (MQ=255)
                                tcggtGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGcc                                                                      <  1:2673620/97‑1 (MQ=255)
                                 cGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGGGGGAGCTGccg                                                                     >  1:4069873/1‑100 (MQ=255)
                                  gATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCg                                                                    <  2:1507281/101‑1 (MQ=255)
                                        ggcggcgCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTTCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCgg                                                              >  2:2272491/1‑101 (MQ=255)
                                         gcggcgCTGGCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTggccgg                                                             >  2:2178784/1‑98 (MQ=255)
                                            gcgcgggCCGGGCGCCGGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGgg                                                          <  2:1925505/95‑1 (MQ=255)
                                              gcTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCa                                                        <  1:3117518/101‑1 (MQ=255)
                                               ctggCCGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCAt                                                       <  1:3990086/98‑1 (MQ=255)
                                                tCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATg                                                      >  1:4297992/1‑101 (MQ=255)
                                                  gCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGc                                                    <  2:4434009/101‑1 (MQ=255)
                                                   ccGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCg                                                   <  1:2907631/101‑1 (MQ=255)
                                                   ccGGGCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCg                                                   >  2:1672080/1‑101 (MQ=255)
                                                     gggCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGat                                                 <  1:2475335/101‑1 (MQ=255)
                                                     gggCGCCTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGat                                                 >  2:1969965/1‑101 (MQ=255)
                                                      ggcggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatc                                                >  2:3287300/1‑101 (MQ=255)
                                                       gcggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatca                                               <  2:3394573/101‑1 (MQ=255)
                                                        cggcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGatcat                                              <  1:4112745/101‑1 (MQ=255)
                                                        cgccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcat                                              >  1:3736209/4‑101 (MQ=255)
                                                         gccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcatc                                             <  1:1615099/99‑1 (MQ=255)
                                                         gccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGatcatc                                             >  1:3167149/3‑101 (MQ=255)
                                                          gcTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCg                                            >  1:3027935/1‑101 (MQ=255)
                                                          ccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCg                                            <  2:2310968/100‑1 (MQ=255)
                                                          ccTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCg                                            >  1:851802/2‑101 (MQ=255)
                                                           cTGATGTGGCATTCGACCGTGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGTTGCCGGTGGCCGTGGGCATGGCGATCATCgg                                           <  1:2524501/101‑1 (MQ=255)
                                                           cTGATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCgg                                           >  1:2835004/1‑101 (MQ=255)
                                                             gATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCg                                         <  1:994987/101‑1 (MQ=255)
                                                             gATGTGGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCg                                         <  1:3238295/101‑1 (MQ=255)
                                                             gATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCg                                         <  2:1306413/101‑1 (MQ=255)
                                                              aTGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGa                                        <  1:2231130/101‑1 (MQ=255)
                                                              aTGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGa                                        >  2:2545506/1‑101 (MQ=255)
                                                               tgtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGGGATCATCGGCggg                                       >  2:264562/1‑99 (MQ=255)
                                                               tgtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAg                                       <  2:4094034/101‑1 (MQ=255)
                                                               tgtgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAg                                       >  1:3946220/1‑101 (MQ=255)
                                                               tgtgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAg                                       >  2:32181/1‑101 (MQ=255)
                                                               ggtgGCATTCGACCGAGGGGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAg                                       <  1:934514/100‑1 (MQ=255)
                                                                 tgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCTGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGc                                     <  2:3348293/101‑1 (MQ=255)
                                                                 tgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGc                                     <  1:1521269/101‑1 (MQ=255)
                                                                 tgGCATTCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGc                                     >  2:1053024/1‑101 (MQ=255)
                                                                 tgGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGc                                     <  2:2985983/101‑1 (MQ=255)
                                                                     aTTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTg                                 >  2:2388097/1‑101 (MQ=255)
                                                                       tCGACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggc                               <  2:668379/101‑1 (MQ=255)
                                                                       tCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAggagatcgg                               >  1:1081781/1‑94 (MQ=255)
                                                                       tCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggc                               <  1:217110/101‑1 (MQ=255)
                                                                       tCGACCGAGGGGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggc                               <  2:1422576/101‑1 (MQ=255)
                                                                        cGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcg                              >  1:2131312/1‑101 (MQ=255)
                                                                         gACCGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggcgg                             >  2:808975/1‑101 (MQ=255)
                                                                         gACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTggcgg                             >  2:129804/1‑101 (MQ=255)
                                                                          tccgatctGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTggcggc                            <  1:1917820/94‑1 (MQ=255)
                                                                           ccGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCt                           <  2:4200621/101‑1 (MQ=255)
                                                                           ccGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCt                           <  1:3004168/101‑1 (MQ=255)
                                                                            cGAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCtt                          >  1:603199/1‑101 (MQ=255)
                                                                             gAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                         >  1:461018/1‑101 (MQ=255)
                                                                             gAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                         >  1:2271262/1‑101 (MQ=255)
                                                                             gAGGTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTa                         >  1:2344444/1‑101 (MQ=255)
                                                                             gAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGCAGCTGCCCGGCCCGGTCGGCATGGGGATCATCGGCGAGCCGGTGGCGGCTTa                         >  2:1454227/1‑101 (MQ=255)
                                                                                gTGCGGGCGCGGCGGCGCCGGTTTTTCGGGCGTGAACTGCTGTGGGAGCTGCCGGTGGCCGTGGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTAttt                      <  2:2690948/101‑1 (MQ=255)
                                                                                   cGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGa                   >  1:175132/1‑101 (MQ=255)
                                                                                     ggCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGc                 <  1:1268193/101‑1 (MQ=255)
                                                                                     ggCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGc                 <  1:3484336/101‑1 (MQ=255)
                                                                                       cgcgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTc               <  2:3893555/101‑1 (MQ=255)
                                                                                       cgcgcAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTc               <  2:4312349/101‑1 (MQ=255)
                                                                                          ccacgccccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGc            <  1:791713/94‑1 (MQ=255)
                                                                                           cAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGTCGGTGGCGGCTTATTTCGAGCTCGGcc           <  1:3682255/101‑1 (MQ=255)
                                                                                           cAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGGGGCGGCTTATTTCGAGCTCGGcc           <  2:1168072/101‑1 (MQ=255)
                                                                                            aaGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCg          >  2:3444530/1‑101 (MQ=255)
                                                                                               cgccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAc       >  2:2939234/1‑101 (MQ=255)
                                                                                               cgccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAc       >  2:3616773/1‑101 (MQ=255)
                                                                                               cgccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAc       >  1:4163690/1‑101 (MQ=255)
                                                                                                cccgtTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGAcc      <  1:1210620/100‑1 (MQ=255)
                                                                                                 cgccTTCGTGAGCCGCGGGCTGCTCTGGGAGCGGCCAGCCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGTCTTATTTCGAGCCCGGCCGGACCg     <  2:2870125/98‑1 (MQ=255)
                                                                                                 ccgcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCg     >  1:436951/1‑101 (MQ=255)
                                                                                                   gcTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTg   <  2:3027714/101‑1 (MQ=255)
                                                                                                    cTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGa  >  1:358444/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACCGAGGTGCGGGCGCGCAAGCGCCGCTTCCTGAGCCGCGAGCTGCTCTGGGAGCTGCCCGTCGCGGTCGGCATGGCGATCATCGGCGAGGCGGTGGCGGCTTATTTCGAGCTCGGCCGGACCGTGA  >  NC_014034/2155464‑2155664

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG