Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 222,006 C→G 0.6% T217T (ACG→ACC RCAP_rcc00185 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034222,0060C→G 0.6% 4.2 ‑1.7 ‑0.41368T217T (ACG→ACCRCAP_rcc00185hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/2):  ref base (699/658):  total (708/660)
Fisher's exact test strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.63e-01

Only 100 of 1704 total aligned reads displayed.

TCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCG  >  NC_014034/221906‑222106
                                                                                                    |                                                                                                    
cggtcgggtccgggggatcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATcc                                                                                                      >  1:3353720‑M1/51‑101 (MQ=255)
        tccgtgggatcggtttgttccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtc                                                                                              >  1:2337173‑M1/43‑101 (MQ=255)
         ccgtgggatcgggcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAtcg                                                                                             >  1:1051091‑M1/42‑101 (MQ=255)
            tgggatcggtcgggtccgtgggatcggtcggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCCGTGGGAtcggtc                                                                                          <  2:3451843‑M1/63‑1 (MQ=255)
            tgggatcggtcgggtccgtgggatcggtcggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                                                          <  2:242841‑M1/63‑1 (MQ=255)
            gcTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                                                                                          >  2:1211352/1‑101 (MQ=255)
             gggatgggtcggctccgttggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtcg                                                                                         >  2:4438679‑M1/38‑101 (MQ=255)
             cTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtcg                                                                                         >  2:1555913/1‑101 (MQ=255)
              tCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcggtcgg                                                                                        >  2:2535860/1‑101 (MQ=255)
               gatcggtcgggtccgtgggatcggtcggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCggg                                                                                       <  2:3845012‑M1/66‑1 (MQ=255)
               cATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCggg                                                                                       >  1:3207773/1‑101 (MQ=255)
                atcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGATCGGTGGGATCGGACGGGt                                                                                      <  2:233983‑M1/67‑1 (MQ=255)
                  cggtcgggtccgtgggatcggtcggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATcc                                                                                    <  2:3903049‑M1/69‑1 (MQ=255)
                   ggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCg                                                                                   <  1:1348872‑M1/70‑1 (MQ=255)
                           gCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcg                                                                           <  1:3741838/101‑1 (MQ=255)
                              tgggatgggtcggtgccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                                        >  1:3828647‑M1/21‑101 (MQ=255)
                              tCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                                        <  1:1116024/101‑1 (MQ=255)
                                    cggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATcc                                                                  <  1:3546489‑M1/87‑1 (MQ=255)
                                     ggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCg                                                                 <  2:784568‑M1/88‑1 (MQ=255)
                                     ggtcgggtccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCg                                                                 >  1:851978‑M1/14‑101 (MQ=255)
                                      gtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGt                                                                <  1:3459143‑M1/89‑1 (MQ=255)
                                      gtcggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGt                                                                <  1:3153483‑M1/89‑1 (MQ=255)
                                      gTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGt                                                                <  2:4083834/101‑1 (MQ=11)
                                        ggtgtcggtcgaATCCGTGGAGCCCGTGGGCTCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGtcg                                                              <  2:2691253/97‑3 (MQ=17)
                                        cggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTgg                                                              <  2:1422164‑M1/91‑1 (MQ=255)
                                          ggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGa                                                            <  1:282336‑M1/93‑1 (MQ=255)
                                          gatccgtgggatgggtcgggtccgtgggatcggtcgggtccgtgGGGTCCGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGa                                                            >  1:2417908/9‑101 (MQ=11)
                                             ccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcg                                                         >  2:1344810‑M1/6‑101 (MQ=255)
                                             ccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGGCGGATCCGTGGGAtcg                                                         <  1:2955516‑M1/96‑1 (MQ=255)
                                             ccgtgGGATCGGCCGGGTCCGTGGGATCGGTGGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGAtcg                                                         >  2:3286083‑M1/6‑101 (MQ=255)
                                              cgtggtatcggtcgggtccgtgggatcggtcgagtccgtgggGGCCGTCGGATGCGTTGGATCGGTCGGAGCCCTGGGATCAGTCCGATCCGCGGGAtcgt                                                        >  1:307578/7‑100 (MQ=2)
                                               gtgggatcgggcgggtccgtgggatcggtcgggtccgtgGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggt                                                       <  1:3959890/98‑1 (MQ=11)
                                               gtgGGATCTGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggt                                                       >  2:529300‑M1/4‑101 (MQ=255)
                                                tgggatcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTGGGATACCTGGGATCGGGGGGGGCGGTGGGATCTGACGGATAGGAGGGAtgtgaa                                                      >  2:850437/3‑96 (MQ=2)
                                                tgggatcggtcgggtccgtcggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGTAtcagtc                                                      >  2:602321/3‑101 (MQ=11)
                                                tgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAtcggtc                                                      >  2:2603267‑M1/3‑101 (MQ=255)
                                                tgGGATCGGTCGGGTCCGTGGGAACGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                                      >  1:1912451‑M1/3‑101 (MQ=255)
                                                 gggatgggtcgggtccgggggatcggtcgggtccgtgGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGGtccgtgg                                                     <  1:2015677/100‑3 (MQ=11)
                                                 gggatcggtcgggtccgtgggatcggtcgggtccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGGCCGTGGGATCGGTCGGCTACGTGGGGtccgtcg                                                     >  2:1294716/2‑101 (MQ=11)
                                                 gggatcggtcggatccgtgggatcggtcggatccgtgGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAtcggtcg                                                     >  1:541664/2‑101 (MQ=11)
                                                   ggtccatcgggtcagtcggagcggtcgggtccgtcggatcggTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCggg                                                   <  2:4353552/95‑1 (MQ=14)
                                                   gatcggtggtgtgcgtgggatgggtgggatccgtggGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggtcgga                                                   <  2:2087427/101‑2 (MQ=11)
                                                    atcggttggatccgtgggatcggtcggatccgtgggATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGAtcggtcggat                                                  <  2:397076/101‑3 (MQ=11)
                                                    atcggtcgggtccgtgggatcggtcgggtccgtgggGTCGGTCGGGTCCGTGGGATCGGCCGGGTCCGTGGGGTCGGTGGGATCGGTGGGGgcgggcggcc                                                  >  2:2199545/1‑99 (MQ=11)
                                                     tcggtcgcgtacgagggatcgggcgggtccgggggaCCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTc                                                 <  2:3668925/101‑1 (MQ=11)
                                                     tccgtcgggtccgtgggatcggtcgggtccgtcggatcgGTCGGGTCCGTGGGATCGGTCGGGCCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTc                                                 >  1:4089349/4‑101 (MQ=14)
                                                     tccgtcgcatccgtggggtccgtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTc                                                 <  2:1004390/92‑1 (MQ=11)
                                                     ccggtcggggccgtgggggcgatcgggtccctgggccCGGCCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTc                                                 <  2:925465/100‑1 (MQ=11)
                                                      ctctctggtccgtgggctctttcggatccgtgggctctgtcgGATCCGTGGGATCTGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTcc                                                <  2:2916142/95‑1 (MQ=11)
                                                       ggtcgggtccgtgggatcggtcgggtccgtgggatcGGTCGGGTCCGTGGGGTCCGTCGGATCCGTAGGGTCGGGCGGATGCGAGGGGTGGGGAGGGTCCg                                               >  2:4250652/1‑101 (MQ=2)
                                                        gtcggatccgtgggatcggtcgggtccgtgggatcgGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGt                                              <  1:4337715/101‑1 (MQ=11)
                                                        gtcggatccgtgggatcggtcggatccgtaggatcgGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGt                                              >  2:1842659/1‑101 (MQ=11)
                                                        gnnnnnnccgtgggatcngtcgggtccgtgggatcgGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGt                                              <  2:1068563/101‑1 (MQ=11)
                                                         tcgggtctgtgggatcggtcgggtccgtgggatcggTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGtg                                             <  2:2283475/101‑2 (MQ=14)
                                                         tcggatccgtgggatcggtctgatccgtgggatcggTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGtg                                             <  2:2823256/101‑2 (MQ=11)
                                                          gtgttccgtgggatcgggcgggtccggggggtccgtcggATCCGTGGGATCGGGCGGAGCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGtgg                                            <  1:3888537/97‑3 (MQ=14)
                                                          cggatccgtgggatcggtcggatccgtgggatcggtCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGtgg                                            >  2:1485280/1‑99 (MQ=11)
                                                              tccgtgggatggttcgggtccgtgggatcggtcggGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGAtc                                        <  2:3523739/101‑1 (MQ=11)
                                                              tccgtgggatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGCCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtc                                        >  1:1742937/1‑101 (MQ=11)
                                                               ccgtgggatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcg                                       >  1:2496926‑M2/1‑94 (MQ=255)
                                                                 gtgggttctgtcgggcccgtgggatcggccggGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggt                                     <  2:4127018/101‑1 (MQ=11)
                                                                  tgggatcggtcggatccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGAtcggtc                                    <  1:2650904/101‑1 (MQ=14)
                                                                  tgggatcggtcggatccgtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGAtcggtc                                    >  1:4018708/1‑101 (MQ=11)
                                                                  taggattggtcgggtccgtaggatccgtcggATCCGTGGGATCCGTCGTGTCCGGTGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGAtcggtc                                    <  2:2756315/99‑1 (MQ=17)
                                                                   gggatcggtcgggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcggtcg                                   <  1:598480‑M2/101‑12 (MQ=255)
                                                                   gggatcggtcggatccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcg                                   <  1:1498729‑M2/101‑12 (MQ=255)
                                                                     gatcggtcgggtccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcggg                                 <  1:694526‑M2/101‑14 (MQ=255)
                                                                      gtcggtcgggtccgtgggatcggtcggaTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTgggatcggtcggat                                >  2:3367764‑M2/2‑87 (MQ=255)
                                                                        cggtctggtccgtggggtccgtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtcc                              >  1:460132/1‑101 (MQ=11)
                                                                        cggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGATCCGTGGGATCGGTCGGGTCCGTggggtcggtcgggtcg                              <  1:1570859‑M2/101‑17 (MQ=255)
                                                                        cgggtgggtccgtgggatcgggcggatccgTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtcc                              >  1:2210165‑M2/6‑85 (MQ=255)
                                                                         ggtcgggtccgtgggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccg                             >  1:4250340‑M2/1‑84 (MQ=255)
                                                                         ggtcgggtccgtgggatcggtcggGTCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGGTTCgtcggatccg                             >  2:322993/1‑101 (MQ=11)
                                                                         gggcgggtccggcggatcggtgggGGGCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccg                             <  1:1677468/98‑1 (MQ=1)
                                                                           tcgggtccgtcggatcggtcggGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtc                           <  1:1284586/101‑1 (MQ=11)
                                                                            cggatccgtgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCGGTCGGGTCGGGGGGGTCGggcggatcggggg                          >  2:685239/1‑96 (MQ=11)
                                                                             gggtccgtgggatcggtcggTTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtggg                         >  2:3011744‑M2/1‑80 (MQ=255)
                                                                             ggatccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcgg                         >  1:3284457/4‑101 (MQ=255)
                                                                             ggatccgtaggatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGGTCGACCGGGTCCGTCGGATCCgtggggtccgtcgg                         >  1:1421556/4‑101 (MQ=11)
                                                                               tgccgtggggtccgttggATCCGTGGGGTCGGTCGGTTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGgtcgggtccgtggggt                       <  1:780274/99‑1 (MQ=11)
                                                                               gtccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggat                       <  2:1924879/101‑3 (MQ=255)
                                                                               atccgtgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGgtcgggtccgtcggat                       <  1:1001929/100‑3 (MQ=255)
                                                                                   gtgggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTcgggtccgtcggatccgt                   >  2:1768918/1‑101 (MQ=11)
                                                                                    tgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcggatccgtgggatcggtc                  >  1:2104033‑M2/1‑73 (MQ=255)
                                                                                    tgggatcggtcggATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGGGGGATCGGCCGGGTCCCTGGGACCGgccggatccgtgggatgggcc                  >  1:2288633/1‑94 (MQ=11)
                                                                                    tgggatcggtcggATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTcgggtccgtcggatcggtc                  <  2:2007889/101‑2 (MQ=11)
                                                                                     gggatcggtcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTgg                 <  2:1137504/101‑1 (MQ=11)
                                                                                      ggatcgggcggATCCGTAGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTCGGATCCGTggg                <  2:3170165/101‑1 (MQ=11)
                                                                                       gatcggtcggGTCCGTAGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCCGTGGGGGCCGTCCGGGCCGTgggg               >  2:3106460/1‑100 (MQ=11)
                                                                                        atcggtcggGTCCGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTAGGATCCGTGGGCTCGCcggggtccctggggtccgccggac              >  2:1649837/1‑95 (MQ=2)
                                                                                         tccgtcggAGCCGTGGGGTCCGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTCGGATCGGTGGGAtc             <  2:4018452/98‑1 (MQ=11)
                                                                                             tgggcgacGTAGGTTCGGTGGGGTCCGTCGGATCGATCGGATCCGTGGAATCGGTCGGGTCCGTCGGATGCGTGGGGTCCGTCGGGTCCGTGGGAtcggtc         <  1:2564235/94‑1 (MQ=14)
                                                                                             gtggggccGGGGGGTCGGTCGGAGCCGTGGGATCCGGCGGATCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGGTCCGTCGGAtccgtg         <  1:441142/95‑2 (MQ=2)
                                                                                                ggtCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGCCGGGTCCGTGGGGAGCGTCGGAtcagtagaa      >  1:2743936/3‑97 (MQ=11)
                                                                                                 gtcCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtggggtccgtcgggtccgtgggag     <  1:1192227‑M2/100‑42 (MQ=255)
                                                                                                  tCCGGGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcggtcggatccgtgggatcggtcggatccgtgggatc    >  1:1818868‑M2/1‑59 (MQ=255)
                                                                                                   ccGTGGGGTCCGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtgggatcggtcgggtccgtgggatcg   <  1:4127278‑M2/101‑44 (MQ=255)
                                                                                                   ccGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTgggatcggtcggatccgtgggatcggtcggatccgtgggatgg   <  1:931482‑M2/101‑44 (MQ=255)
                                                                                                   ccGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTgggatcggtcgggtccgtgggatcggtcggatccgtgggatcg   <  2:3742720‑M2/101‑44 (MQ=255)
                                                                                                    cGTGGGATCGGTCGGATCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTgggatcggtcggatccgtgggatcggtcgggtccgtgggatcgg  <  1:3657948‑M2/101‑45 (MQ=255)
                                                                                                    |                                                                                                    
TCGCCGGATCGCGCTCATCGACGGTGGGCGTCGGATGCGTGGGCTCGGTCGGATCGGTCGGGTCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTGGGATCGGTCGGATCCGTGGGATCGGTCGGGTCCGTAGGATCGGTCGGGTCCGTCGGGTCCGTGGGATCGGTCGGATCCG  >  NC_014034/221906‑222106

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG