Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,537,516 T→A 100% R84W (AGG→TGG)  mdoG2 ← glucans biosynthesis protein G

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,537,5160T→A100.0% 748.4 186R84W (AGG→TGG) mdoG2glucans biosynthesis protein G
Reads supporting (aligned to +/- strand):  new base (107/79):  ref base (0/0):  total (107/79)

Only 100 of 190 total aligned reads displayed.

CATCTTCACCGGCTGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCTATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGCGCGGGCGGCCAGC  >  NC_014034/2537418‑2537613
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cATCTTCACCGGCTGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCCGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAAt                                                                                                 >  1:3837367/1‑101 (MQ=255)
  tCTTCACCGGCTGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCCATgc                                                                                               >  2:1031676/1‑101 (MQ=255)
  tCTTCACCGGCTGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATgc                                                                                               <  1:117171/101‑1 (MQ=255)
  tCTTCACCGGCTGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATgc                                                                                               >  1:2333033/1‑101 (MQ=255)
       aCCGGCTGGCGGAAATAGGGGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTTTCCTTGCGGGAGCGGATCTGCCAATGcgcgcc                                                                                          >  2:2267851/1‑99 (MQ=255)
        ccGGCTGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGcgtcg                                                                                         >  1:1833245/1‑101 (MQ=255)
          ggCTGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTa                                                                                       <  2:3259240/101‑1 (MQ=255)
           gCTGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTaa                                                                                      <  2:3003918/101‑1 (MQ=255)
            cTGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAAt                                                                                     <  1:1259377/101‑1 (MQ=255)
             tGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATc                                                                                    >  1:194023/1‑101 (MQ=255)
              ggCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCg                                                                                   >  1:1630471/1‑101 (MQ=255)
               gCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGa                                                                                  <  2:2661951/101‑1 (MQ=255)
                cGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGAt                                                                                 <  2:2771877/101‑1 (MQ=255)
                cGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGAt                                                                                 >  1:1690265/1‑101 (MQ=255)
                 ggAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATg                                                                                <  1:3040322/101‑1 (MQ=255)
                 ggAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATg                                                                                >  2:3835826/1‑101 (MQ=255)
                 ggAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATg                                                                                >  2:3007990/1‑101 (MQ=255)
                   aaaTAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATgcg                                                                              >  1:4139957/1‑101 (MQ=255)
                   aaaTAGGTGCCCAGATGGAAGAACGGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATgcg                                                                              <  2:2245039/101‑1 (MQ=255)
                    aaTAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATgcgc                                                                             >  1:4111316/1‑101 (MQ=255)
                      tAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGTTCTGCCAATGCGCGTCGTAATCGATGCGCTc                                                                           <  1:3957071/101‑1 (MQ=255)
                      tAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATGTGCCAATGCGCGTCGTAATCGATGCGCTc                                                                           >  2:3305332/1‑101 (MQ=255)
                      tAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTc                                                                           >  2:2852652/1‑101 (MQ=255)
                        ggTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCa                                                                         >  1:753013/1‑101 (MQ=255)
                          tGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGGGCTCCAGc                                                                       >  2:1652118/1‑101 (MQ=255)
                           gCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCa                                                                      >  1:2150557/1‑101 (MQ=255)
                             ccAGATGGCAGAACTGCACGGGCACGGCGCCCAGCTGCCGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCAcc                                                                    >  1:3002013/1‑101 (MQ=255)
                             ccAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCAcc                                                                    >  2:932568/1‑101 (MQ=255)
                              cAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCt                                                                   >  2:2137907/1‑101 (MQ=255)
                               aGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTc                                                                  >  2:1312347/1‑101 (MQ=255)
                               aGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTc                                                                  >  1:3167028/1‑101 (MQ=255)
                               aGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCAACTc                                                                  >  2:472772/1‑101 (MQ=255)
                                gATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCg                                                                 >  2:1857462/1‑101 (MQ=255)
                                 aTGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCgg                                                                >  2:3743590/1‑101 (MQ=255)
                                 aTGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCgg                                                                <  2:4080087/101‑1 (MQ=255)
                                   ggAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGc                                                              >  1:1331606/1‑101 (MQ=255)
                                    gaagaaCTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCt                                                             <  1:4436002/101‑1 (MQ=255)
                                     aagaaCTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTg                                                            <  1:488431/101‑1 (MQ=255)
                                      agaaCTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTgg                                                           >  1:3936426/1‑101 (MQ=255)
                                       gaaCTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGTGCGGCGTAATCGATGCGCTCCAGCACCCGGGGCTggg                                                          >  1:3803858/1‑100 (MQ=255)
                                         aCTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGgcgc                                                        <  2:4262462/101‑1 (MQ=255)
                                           tGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCgcgg                                                      <  1:675836/101‑1 (MQ=255)
                                            gcacgggcacggCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCgcggc                                                     <  1:1031764/101‑1 (MQ=255)
                                             cacgggcacggCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCgcggcg                                                    >  2:135188/1‑101 (MQ=255)
                                                gggcacggCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCgcggcggcg                                                 >  1:2475854/1‑101 (MQ=255)
                                                 ggcacggCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGgcgc                                                <  1:2682879/101‑1 (MQ=255)
                                                   cacggCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCgg                                              >  1:3636907/1‑101 (MQ=255)
                                                    actgcgcCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCggg                                             >  1:1571666/4‑101 (MQ=255)
                                                    acggCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCggg                                             >  1:3571570/1‑101 (MQ=255)
                                                     cggCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGc                                            >  2:2089936/1‑101 (MQ=255)
                                                      ggCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGca                                           >  1:2916191/1‑101 (MQ=255)
                                                        cgcCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGcaca                                         >  2:1715510/1‑101 (MQ=255)
                                                         gcCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACAt                                        >  1:2714975/1‑101 (MQ=255)
                                                         gcCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACAt                                        >  1:3129522/1‑101 (MQ=255)
                                                          cccAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATa                                       >  2:2375290/1‑101 (MQ=255)
                                                           ccAGCTGCAGCGTCTTGTCCTTGCGGTGGCGGATCTGCCAACGCGGGTCGTAATCGTGGCGCTCCAGCACCGCGGGCTGGCGCGGCGGCGCGGGCACATAg                                      >  2:3985865/1‑101 (MQ=255)
                                                            tttcgatctcGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAgg                                     <  2:2239299/92‑1 (MQ=255)
                                                            cAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAgg                                     <  2:2912974/101‑1 (MQ=255)
                                                             aGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAggg                                    >  2:2264381/1‑101 (MQ=255)
                                                              gCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgc                                   <  2:59960/101‑1 (MQ=255)
                                                               cTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgcg                                  <  2:1828874/101‑1 (MQ=255)
                                                                tGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgcgc                                 >  2:39186/1‑101 (MQ=255)
                                                                tGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgcgc                                 >  1:1531614/1‑101 (MQ=255)
                                                                tGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgcgc                                 <  2:2150418/101‑1 (MQ=255)
                                                                tGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgcgc                                 >  1:2770098/1‑101 (MQ=255)
                                                                tGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgcgc                                 >  1:948563/1‑101 (MQ=255)
                                                                 gCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgcgcg                                >  1:62037/1‑101 (MQ=255)
                                                                 gCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgcgcg                                <  2:193996/101‑1 (MQ=255)
                                                                 gCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgcgcg                                >  1:322849/1‑101 (MQ=255)
                                                                  cAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGgcgcgc                               >  1:1400361/1‑101 (MQ=255)
                                                                   aGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGcgcgcg                              >  2:1625566/1‑101 (MQ=255)
                                                                   aGCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGcgcgcg                              >  2:2899093/1‑101 (MQ=255)
                                                                    gCGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCgg                             <  2:1220712/101‑1 (MQ=255)
                                                                     cGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCggg                            >  1:2784464/1‑101 (MQ=255)
                                                                     cGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCggg                            >  2:1778350/1‑101 (MQ=255)
                                                                     cGTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCggg                            >  2:693302/1‑101 (MQ=255)
                                                                      gTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGc                           <  2:1988842/101‑1 (MQ=255)
                                                                      gTCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGc                           <  2:3139546/101‑1 (MQ=255)
                                                                       tCTTGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATACGGCGCGCGGGCg                          >  2:1781602/1‑101 (MQ=255)
                                                                         ttGTCCTTGCGGTAGCGGATCTGCCAATGCTCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGAt                        >  1:3428693/1‑101 (MQ=255)
                                                                         ttGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGAt                        >  2:4335336/1‑101 (MQ=255)
                                                                          tGTCCTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATg                       >  2:43779/1‑101 (MQ=255)
                                                                             ccTTGCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATggcg                    >  1:2039613/1‑101 (MQ=255)
                                                                                 gCGGTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATggcggcgg                <  1:3769740/101‑1 (MQ=255)
                                                                                   tccgatctaTCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGgcggcggcg              <  2:1089613/93‑1 (MQ=255)
                                                                                   ggTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGgcggcggcg              >  2:4115098/1‑101 (MQ=255)
                                                                                   ggTAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGgcggcggcg              >  1:1678344/1‑101 (MQ=255)
                                                                                     tAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGcgcg            <  1:2226633/101‑1 (MQ=255)
                                                                                     tAGCGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGcgcg            <  1:3008211/101‑1 (MQ=255)
                                                                                        cGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGTTGGCgagggg         >  1:3739514/1‑100 (MQ=255)
                                                                                        cGGATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGCgcgggc         <  2:4060052/101‑1 (MQ=255)
                                                                                          gATCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGCgcgggcgg       <  1:2401757/101‑1 (MQ=255)
                                                                                           aTCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGgcggcggcgagatcgga      >  1:1089701/1‑93 (MQ=255)
                                                                                           aTCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGCGCGggcggc      >  2:3482117/1‑101 (MQ=255)
                                                                                            tCTGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGCGCGGGCGGcc     >  1:2442781/1‑101 (MQ=255)
                                                                                              tGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGCGCGGGCGGCCAg   <  1:3373240/101‑1 (MQ=255)
                                                                                              tGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGCGCGGGCGGCCAg   >  1:4090583/1‑101 (MQ=255)
                                                                                              tGCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGC.CCGGCGGcccac  >  1:2658565/1‑98 (MQ=255)
                                                                                               gCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGCGCGGGCGGCCAgc  <  2:2518658/101‑1 (MQ=255)
                                                                                               gCCAATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGCGCGGGCGGCCAgc  <  2:1252883/101‑1 (MQ=255)
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CATCTTCACCGGCTGGCGGAAATAGGTGCCCAGATGGAAGAACTGCACGGGCACGGCGCCCAGCTGCAGCGTCTTGTCCTTGCGGTAGCGGATCTGCCTATGCGCGTCGTAATCGATGCGCTCCAGCACCTCGGGCTGGCGCGGCGGCGCGGGCACATAGGGCGCGCGGGCGATGGCGGCGGCGCGGGCGGCCAGC  >  NC_014034/2537418‑2537613

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG