BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,566,555	C→T	100%	intergenic (+27/+167)	purD → / ← sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,566,555	0	C→T	100.0%	405.8	143	intergenic (+27/+167)	purD/sdaA	phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand): new base (38/103): ref base (0/0): total (38/105)

Only 100 of 153 total aligned reads displayed.


ATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GGGGGCGCT.GCCCCC.CTC.CT..G..CTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGC  >  NC_014034/2566458‑2566651


gtgcgagaggcGATGCGCTCGACTGGCCCGAGGGGTTTTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..g                                                                                                    <  2:1442056/91‑1 (MQ=255)


aTGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..g                                                                                                    <  2:1717287/101‑1 (MQ=255)


aTGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..g                                                                                                    <  2:2930986/101‑1 (MQ=255)


 tGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..gg                                                                                                   <  2:3120958/101‑2 (MQ=255)


   cGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGGGGCCGCTCTGAGAGG.GAAC.GGGGGGGGG.GCCCCc.ccc.cctgtgc                                                                                                  >  2:596607/1‑95 (MQ=255)


    gagggTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..ggcct                                                                                                <  2:1539579/98‑5 (MQ=38)


     aTGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGGGCGCGCTCTGAGAAGAAAAC.GGGGGCGCC.GCCCCc.cgc.cttggact                                                                                                 >  2:1417272/1‑95 (MQ=255)


      tGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..ggccttc                                                                                              <  1:1302888/101‑7 (MQ=38)


      tGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..ggccttc                                                                                              <  2:2142979/101‑7 (MQ=38)


       ggTGGATGCGATCGACTGGCCCGGGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGGCG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..ggccttcg                                                                                             <  2:1433428/101‑8 (MQ=37)


                 aTCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCAc                                                                                   <  2:1593355/101‑1 (MQ=37)


                   cGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCAccc                                                                                 <  2:2502891/101‑1 (MQ=37)


                     aCTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGGGCGCGGTCTGAGACG.AAACGGGGGGCGCC.GGCCCC.CTC.TT..GGCCTTGGGCCCATTCAccccc                                                                               >  1:3689634/1‑101 (MQ=255)


                      cTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCAcccccc                                                                              <  2:193483/101‑1 (MQ=25)


                      cTGGCCAGAGGGGTTAAGCCGACACAACATCGGCTGGCGCGAGCTCTGAGAAG.AAACGGGGGGCGCT.GCCCCC.ATC.TT..GGCCTTCGGCCAATTCAcccccc                                                                              <  2:4392946/101‑1 (MQ=12)


                       tGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCCGAGACG.AAACGGGGGGCGCT.CCCCCC.CCC.TTGGG..CCTCCGCCCATTCACCCCCCg                                                                             >  2:2784179/1‑101 (MQ=255)


                        ggCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCCATTCAcccccccg                                                                            >  2:3289298/1‑99 (MQ=25)


                         gCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGGC.GCCCCC.CTC.TTGGG..CTTCGGGCAATTCACCCCCCcgg                                                                           >  2:2311527/1‑101 (MQ=255)


                         gCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCggg                                                                           <  2:821208/101‑1 (MQ=25)


                           ccGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCCATTCACCCCCCCGGat                                                                         >  2:3591090/1‑101 (MQ=25)


                           ccGAGGGGTTATGCCGCCGCGACCTCGGCTGGCGCGCGCTCTGAGTCG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGat                                                                         <  1:3406132/101‑1 (MQ=21)


                            cgagctgTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGata                                                                        <  2:658737/95‑1 (MQ=21)


                            cGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCCCCCCCCCGGgta                                                                        >  1:3267721/1‑101 (MQ=21)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGGGGTGGCCCCC.CCT.TT..GC.CTTCGGCCCATTCACCCCCCcggcaat                                                                       >  2:3764620/1‑97 (MQ=255)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGata.t                                                                      <  2:832863/101‑1 (MQ=25)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGata.t                                                                      <  2:8710/101‑1 (MQ=25)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGata.t                                                                      <  1:1022552/101‑1 (MQ=25)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGata.t                                                                      <  1:2581725/101‑1 (MQ=25)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCCGGata.t                                                                      >  1:2267035/1‑101 (MQ=25)


                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGCCCAATTCACCCCCCGGGata.t                                                                      <  1:3286874/101‑1 (MQ=25)


                                gggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTc                                                                   <  2:716590/101‑1 (MQ=21)


                                gggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTc                                                                   <  1:149209/101‑1 (MQ=21)


                                   ttCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCggg                                                                <  1:2045935/101‑1 (MQ=21)


                                      tGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCaa                                                             <  1:1757897/101‑1 (MQ=21)


                                      tGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCCCT.TCCCCC.CTCTTT..GC.CTTCGGCCAATTCACCCCCCGCGGTA.TTTCGGGGaa                                                             >  2:1266654/1‑101 (MQ=21)


                                       gccgccGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTCTTT..GC.CTTCGGCCAATTCACCCCCCCGGATA.TTTCTGGGAAg                                                            >  2:3968143/1‑101 (MQ=21)


                                       gccgccGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAg                                                            <  2:3544526/101‑1 (MQ=21)


                                        ccgccgCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCCCCCCCCGGGATA.TTTCGGGCAAGa                                                           <  1:4402108/101‑1 (MQ=21)


                                        ccgccgCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGa                                                           <  1:768504/101‑1 (MQ=21)


                                         cgccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGAt                                                          <  1:2301070/101‑1 (MQ=21)


                                          gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATa                                                         <  1:1595489/101‑1 (MQ=21)


                                          gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATa                                                         <  1:3625649/101‑1 (MQ=21)


                                           ccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATaa                                                        <  2:2601768/101‑1 (MQ=21)


                                            cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATaaa                                                       <  2:3837105/101‑1 (MQ=21)


                                            cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATaaa                                                       <  2:1980484/101‑1 (MQ=21)


                                            cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAAATaaa                                                       <  1:295598/101‑1 (MQ=21)


                                             tcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TCTCGGGCAAGATAaag                                                      <  1:3750265/100‑1 (MQ=21)


                                             gcgACATCGGCTGGCGCGCGCTCTGAGACGAAAAG.GGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCCGCCAATTCACCCCCCCGGGTA.TTTCGGGGAAGATaaaa                                                      >  2:3461737/1‑100 (MQ=18)


                                             gcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGTCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAaag                                                      <  1:1358392/101‑1 (MQ=21)


                                               gACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAaagaa                                                    <  2:3920021/101‑1 (MQ=21)


                                               gACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAaagaa                                                    <  2:1061428/101‑1 (MQ=21)


                                               gACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.ATTCGGGCAAGATAaagaa                                                    >  1:2318559/1‑101 (MQ=21)


                                                aCATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAaaaaag                                                   >  1:1271813/1‑101 (MQ=21)


                                                 cATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAgg                                                  <  1:215566/101‑1 (MQ=21)


                                                 cATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAgg                                                  <  1:2985799/101‑1 (MQ=21)


                                                     ggCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCtt                                              <  2:1394035/101‑1 (MQ=21)


                                                     gacTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCtt                                              >  1:3966902/3‑101 (MQ=21)


                                                      gCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCttt                                             >  1:4154432/1‑101 (MQ=21)


                                                       cTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCtttt                                            <  1:885005/101‑1 (MQ=21)


                                                       cTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCtttt                                            <  2:327401/101‑1 (MQ=21)


                                                       cTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCtttt                                            <  1:4081669/101‑1 (MQ=21)


                                                        tGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCGCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTc                                           <  1:2647695/101‑1 (MQ=21)


                                                        tGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGGTA.ATTTGGGCAAGATAAAAAAggggctttt                                           >  1:2392665/1‑94 (MQ=12)


                                                        tGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTc                                           >  2:3361660/1‑101 (MQ=21)


                                                        tGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCCGGGTATTTTCGGGC.AGATAaaaaaagggttttt                                           >  2:3027699/1‑92 (MQ=9)


                                                          gcgcgcgcTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCt                                         <  2:53519/101‑1 (MQ=21)


                                                           cgcgcgcTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                        <  2:1094175/101‑1 (MQ=21)


                                                            gcgcgcTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                       <  1:3001361/101‑1 (MQ=21)


                                                             cgcgcTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                      <  1:1807017/101‑1 (MQ=21)


                                                             cgcgcTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGGCTTTTCCTTTc                                      >  2:413218/1‑101 (MQ=21)


                                                              gcgcTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                     <  2:124700/101‑1 (MQ=21)


                                                               cgcTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                    <  1:3596700/101‑1 (MQ=21)


                                                                gcTCTGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCCCCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                   <  1:817442/101‑1 (MQ=21)


                                                                   ctGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                >  1:1274440/1‑101 (MQ=21)


                                                                   ctGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                >  2:2689914/1‑101 (MQ=21)


                                                                   ctGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCAAATTCGCCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                <  1:4265530/101‑1 (MQ=21)


                                                                    tGAGACG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGAAA.TTTCCTGCAAGATAAAGAAGGTCTTTTTCTTTCATCTTGc                               >  1:495608/1‑101 (MQ=18)


                                                                     gagaCG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTGCCTTTCATCTTGcc                              <  1:519025/101‑1 (MQ=21)


                                                                       gaCG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                            <  1:1785610/101‑1 (MQ=21)


                                                                       gaCG.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                            >  1:1777787/1‑101 (MQ=21)


                                                                          g.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCCATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                         <  1:3107135/101‑1 (MQ=21)


                                                                          g.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTTGGGGAAGATAAAGAAGGGCTTTTCCTTTTATCTTTCCCCaaa                         >  1:3883112/1‑101 (MQ=17)


                                                                          g.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                         <  2:4135017/101‑1 (MQ=21)


                                                                          g.AAACGGGGGGCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGAAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                         >  2:1377123/1‑101 (MQ=21)


                                                                            aaacggggggCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCTCCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                        <  1:4394270/96‑1 (MQ=21)


                                                                            aaacggggggCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAat                        <  1:661025/96‑1 (MQ=21)


                                                                             aacggggggCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                       >  1:2794101/5‑101 (MQ=25)


                                                                             aacggggggCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                       <  1:3403555/97‑1 (MQ=25)


                                                                              acggggggCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                      <  1:1158219/98‑1 (MQ=25)


                                                                              acggggggCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                      >  2:4193579/4‑101 (MQ=25)


                                                                                agggggCGCT.G.CCCC.CTC.TT..GGCCTTTGGCCAATTCA.CCCCTGGGATA.TTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCg.gggg             >  1:3465033/2‑101 (MQ=0)


                                                                                 gggggCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                   <  1:2802997/101‑1 (MQ=25)


                                                                                 gggggCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                   <  1:2729715/101‑1 (MQ=25)


                                                                                 gggggCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATGCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                   >  1:4105956/1‑101 (MQ=25)


                                                                                  ggggCGCT.GCCCCCTCTT.GG..C..CTTTGGCCAATTCA.CCCCTGGGATA.TTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCg.ggggga           <  2:2846502/101‑3 (MQ=2)


                                                                                   gggCGCT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg.g                <  1:3108485/101‑1 (MQ=37)


                                                                                       gcT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGa            <  1:2932405/101‑1 (MQ=37)


                                                                                        cT.GCCCCC.CTC.TT..GGCCTTCGGCCAATTCACCCCCCGGGATA.CTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAg           >  2:3937016/1‑101 (MQ=37)


                                                                                           gCCCCC.CTC.TT..GGCCTTCGGCCAATTCCCCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGcc         <  1:693658/101‑1 (MQ=38)


                                                                                            ccccc.cTC.TG..G..CTTC.GCC.ATTCACCCCCCGGGATA.TTTCGGGCACGATGA..AAGG.CATCCGGTTTCATCTTGCTGCAAATATCCCGAGGGGAGGCCGATTgc  >  2:2946351/1‑101 (MQ=18)


ATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GGGGGCGCT.GCCCCC.CTC.CT..G..CTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCG.GGGGAGCCCGCTTGC  >  NC_014034/2566458‑2566651

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG