Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,223,935	G→C	100%	P129A (CCC→GCC)	fdhD ←	formate dehydrogenase accessory protein FdhD

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,223,935	0	G→C	100.0%	43.8	12	P129A (CCC→GCC)	fdhD	formate dehydrogenase accessory protein FdhD
Reads supporting (aligned to +/- strand):  new base (3/9):  ref base (0/0):  total (3/9)

CGCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGGGC  >  NC_014034/3223839‑3223954                                                                                                 |                    cgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggg                 <  1:3164662/101‑1 (MQ=255)  gcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcggggg                <  2:247665/101‑1 (MQ=255)   ccGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgc               <  1:1719834/101‑1 (MQ=255)   ccGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgc               <  2:661757/101‑1 (MQ=255)   ccGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGAACCATCGGCCAGGGGGGCGGGGgc               <  1:2004195/101‑1 (MQ=255)    cGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcg              >  2:2582653/1‑100 (MQ=255)     gTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgc             <  1:4312919/101‑3 (MQ=255)       cccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcca           <  1:2128682/101‑2 (MQ=255)        ccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcggcca          >  2:3074533/1‑96 (MQ=255)            aaCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgc      >  1:4222846/1‑95 (MQ=255)              ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca    <  1:1418559/101‑9 (MQ=255)              ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca    <  1:4345349/101‑9 (MQ=255)               cccGCCGCAGGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccag   <  1:1807635/101‑10 (MQ=255)                ccgccgCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccagc  <  1:256185/101‑11 (MQ=255)                                                                                                 |                    CGCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGGGC  >  NC_014034/3223839‑3223954 Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG