Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,392 G→C 92.6% intergenic (‑102/+28) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3920G→C92.6% 12.1 ‑3.3 ‑0.9124intergenic (‑102/+28)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (57/56):  ref base (8/1):  total (67/57)
Fisher's exact test strand distribution p-value = 3.58e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.34e-01

Only 100 of 132 total aligned reads displayed.

CGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCG  >  NC_014034/3461307‑3461487
                                                                                       |                                                                                                
    cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                                          <  2:2107219/101‑1 (MQ=255)
     ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                                         <  2:2858808/101‑1 (MQ=255)
     ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                                         <  2:2679688/101‑1 (MQ=255)
     ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                                         <  2:4343925/101‑1 (MQ=255)
     ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                                                         >  2:533440/1‑101 (MQ=255)
      cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                                                        <  1:1719150/101‑1 (MQ=255)
       gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                                                       <  2:1952006/101‑1 (MQ=255)
        cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                                                      >  2:2947658/1‑101 (MQ=255)
        cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                                                      >  1:996430/1‑101 (MQ=255)
        cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                                                      <  1:2517397/101‑1 (MQ=255)
         aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                                                     <  2:3413417/101‑1 (MQ=255)
         aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                                                     >  2:202186/1‑101 (MQ=255)
         aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                                                     <  1:3417163/101‑1 (MQ=255)
          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                                                    >  2:4416418/1‑101 (MQ=255)
          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GC..AGCCAACC.GGCCGCGCCCCCAACCCCGGTCGTTCGATc                                                                    >  1:3211255/1‑101 (MQ=255)
           cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                                                   >  1:3494710/1‑101 (MQ=255)
           cTTAGAAAGATGCGCGGGGCCGCCCCGCCGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCCCAGCC.ACCCCGCCG...CG..CCCCCAACCCCGGTCGTTCGATCa                                                                   <  2:567523/101‑1 (MQ=255)
            ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                                                  >  1:564622/1‑101 (MQ=255)
            ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                                                  >  2:3713298/1‑101 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                 >  1:3730562/1‑101 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                 <  1:520629/101‑1 (MQ=255)
              aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CGC.CCCCC.ACCCCGGTCGTTCGATCAGtt                                                                >  1:4339176/1‑101 (MQ=255)
              aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                >  1:3207027/1‑101 (MQ=255)
              aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCCTTCGATCAGtt                                                                >  2:2135246/1‑101 (MQ=255)
              aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGACGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                                                >  2:1817176/1‑101 (MQ=255)
                cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                                              >  1:708295/1‑101 (MQ=255)
                 aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                                             <  2:3955126/101‑1 (MQ=255)
                   gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                           >  2:2197475/1‑101 (MQ=255)
                   gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                           >  1:2642963/1‑101 (MQ=255)
                    aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                                          >  1:566592/1‑101 (MQ=255)
                    aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                                          >  1:1754427/1‑101 (MQ=255)
                    aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                                          >  2:2675822/1‑101 (MQ=255)
                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                         >  2:3434680/1‑101 (MQ=255)
                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                         >  1:4204945/1‑101 (MQ=255)
                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                         <  2:1244882/101‑1 (MQ=255)
                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                         <  2:51690/101‑1 (MQ=255)
                      gCACGGGCGCGGTCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                                                        >  1:2424205/1‑101 (MQ=255)
                      gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                                                        <  1:2848254/101‑1 (MQ=255)
                      gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGGCGGTCGATCAGTTCTTCCcga                                                        >  2:2722907/1‑101 (MQ=255)
                       cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                       <  2:3474612/101‑1 (MQ=255)
                       cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                       <  2:2192517/101‑1 (MQ=255)
                       cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                                                       <  1:4440837/101‑1 (MQ=255)
                        aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                                                      >  2:3756480/1‑101 (MQ=255)
                        aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                                                      >  1:1807860/1‑101 (MQ=255)
                         cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGCTCAGTTCTTGCcgacga                                                     >  1:743932/1‑101 (MQ=255)
                          gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                    >  2:2858817/1‑101 (MQ=255)
                          gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                    >  1:3089612/1‑101 (MQ=255)
                          gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                    >  1:485732/1‑101 (MQ=255)
                           ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                                                   >  1:821679/1‑101 (MQ=255)
                           ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                                                   <  1:2586714/101‑1 (MQ=255)
                            gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCTGGTCGTTCGATCAGTTCTTGCCGACGATGa                                                  <  2:3976994/101‑1 (MQ=255)
                             cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                 >  1:3910591/1‑101 (MQ=255)
                             cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                 <  2:3867993/101‑1 (MQ=255)
                              gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                                                <  1:4051574/101‑1 (MQ=255)
                               cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                                               <  2:2883855/101‑1 (MQ=255)
                                ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                              >  2:2125424/1‑101 (MQ=255)
                                  ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTccg                                            >  2:3184684/1‑99 (MQ=255)
                                   cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                           <  1:3936270/101‑1 (MQ=255)
                                   cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                           <  1:1596684/101‑1 (MQ=255)
                                    aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                          <  1:1563014/101‑1 (MQ=255)
                                    aGACGAAGTTTGGCGCAGGGCCAAAGGAA.GT.GT.GC.GCAGGCAC..CCGGGCGCG.CCCCCAACCCCGGTCGTTCTATCAGTTCTTTGCGACGATGACGTCagag                                          >  2:738617/1‑101 (MQ=255)
                                     gACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                                         >  2:4120964/1‑101 (MQ=255)
                                     gACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                                         >  1:276565/1‑101 (MQ=255)
                                      aCGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCTGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGc                                        <  2:2295402/101‑1 (MQ=255)
                                        gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt                                      >  1:4432896/1‑101 (MQ=255)
                                         cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt                                     <  2:622617/101‑1 (MQ=255)
                                         cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt                                     <  1:274372/101‑1 (MQ=255)
                                          aGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                    <  1:913877/101‑1 (MQ=255)
                                          aGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                    >  1:3418041/1‑101 (MQ=255)
                                           gTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga                                   >  2:352415/1‑101 (MQ=255)
                                           gTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga                                   <  1:1243131/101‑1 (MQ=255)
                                           gTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga                                   >  1:1232571/1‑101 (MQ=255)
                                           gTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga                                   >  1:1066661/1‑101 (MQ=255)
                                            tttGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat                                  <  2:154270/101‑1 (MQ=255)
                                              tGGCGCAGGGCCAAAGGAAGGGAGC.GCAGCC.ACCCTGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                <  2:743852/101‑1 (MQ=255)
                                              tGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCCGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                >  2:1792325/1‑101 (MQ=255)
                                              tGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                >  1:270951/1‑101 (MQ=255)
                                              tGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                <  2:2943594/101‑1 (MQ=255)
                                               ggCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                               >  2:1589245/1‑101 (MQ=255)
                                                 cgcAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg                             >  1:1282155/1‑101 (MQ=255)
                                                 cgcAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg                             >  1:2120997/1‑101 (MQ=255)
                                                  gcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc                            <  1:363640/101‑1 (MQ=255)
                                                  gcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc                            >  2:4175234/1‑101 (MQ=255)
                                                  gcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc                            >  1:3375662/1‑101 (MQ=255)
                                                                        gggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGcc             >  1:423160/11‑101 (MQ=255)
                                                                         ggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCg            <  1:1558551/92‑1 (MQ=255)
                                                                          gggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCgc           >  2:1530925/9‑100 (MQ=255)
                                                                             cgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCt        >  1:676497/6‑101 (MQ=255)
                                                                             cgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCt        <  2:564570/96‑1 (MQ=255)
                                                                              gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCtt       >  2:1701005/5‑101 (MQ=255)
                                                                               cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc      <  2:4204684/98‑1 (MQ=255)
                                                                               cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc      <  2:66609/98‑1 (MQ=255)
                                                                                agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTcg     >  1:2125543/3‑100 (MQ=255)
                                                                                agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa     >  2:2821300/3‑101 (MQ=255)
                                                                                agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa     <  1:2255717/99‑1 (MQ=255)
                                                                                agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa     >  2:4320305/3‑101 (MQ=255)
                                                                                agccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCa     <  1:3434919/99‑1 (MQ=255)
                                                                                  ccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgc   >  2:1105312/1‑101 (MQ=255)
                                                                                   cACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgcg  >  1:1900479/1‑101 (MQ=255)
                                                                                   cACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgcg  >  2:704230/1‑101 (MQ=255)
                                                                                       |                                                                                                
CGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCG  >  NC_014034/3461307‑3461487

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG