BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	539,743	T→G	100%	Y201D (TAC→GAC)	RCAP_rcc00505 →	type II secretion system protein E

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	539,743	0	T→G	100.0%	853.2	229	Y201D (TAC→GAC)	RCAP_rcc00505	type II secretion system protein E
Reads supporting (aligned to +/- strand): new base (112/117): ref base (0/0): total (112/117)

Only 100 of 235 total aligned reads displayed.


CGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACAA  >  NC_014034/539645‑539840


cGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGAc                                                                                                 >  1:3239887/1‑101 (MQ=255)


cGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGAc                                                                                                 >  1:939290/1‑101 (MQ=255)


     gTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCg                                                                                            >  2:2556980/1‑101 (MQ=255)


      tGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGt                                                                                           <  1:2310084/101‑1 (MQ=255)


         ccTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTg                                                                                        >  1:2011046/1‑101 (MQ=255)


         ccTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTg                                                                                        <  2:2414932/101‑1 (MQ=255)


           ttCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGCCGCCCGTCTGGc                                                                                      <  1:2428766/101‑1 (MQ=255)


           ttCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGc                                                                                      <  1:4418474/101‑1 (MQ=255)


            tCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGcc                                                                                     >  1:3917711/1‑101 (MQ=255)


            tCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGcc                                                                                     >  1:2976205/1‑101 (MQ=255)


             taaGGATGAAAAGCTCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCg                                                                                    <  2:3689491/100‑1 (MQ=255)


              aaGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGa                                                                                   <  1:4283788/101‑1 (MQ=255)


               aGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGAt                                                                                  <  1:577300/101‑1 (MQ=255)


               aGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGAt                                                                                  >  2:1184427/1‑101 (MQ=255)


               aGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGAt                                                                                  <  2:1378464/101‑1 (MQ=255)


               aGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGAt                                                                                  <  1:3274312/101‑1 (MQ=255)


                ggATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATg                                                                                 >  2:27488/1‑101 (MQ=255)


                  aTGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGc                                                                               <  1:4260927/101‑1 (MQ=255)


                   tGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCt                                                                              <  2:3281733/101‑1 (MQ=255)


                   tGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCt                                                                              >  1:540032/1‑101 (MQ=255)


                   tGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTAGGTCGACGCCCGTCTGGCCGATGGCt                                                                              <  2:2081946/101‑1 (MQ=255)


                    gAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATggtct                                                                             <  2:3192032/101‑4 (MQ=255)


                     aaaaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGcgct                                                                            <  2:393568/101‑4 (MQ=255)


                     aaaaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcg                                                                            >  2:1938311/1‑101 (MQ=255)


                     aaaaGCACCTGTTGCGGATCATCGACAAGATAGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcg                                                                            >  1:4014329/1‑101 (MQ=255)


                      aaaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcgc                                                                           >  1:609480/1‑101 (MQ=255)


                       aaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcgcg                                                                          <  2:4173511/101‑1 (MQ=255)


                       aaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcgcg                                                                          <  2:1675691/101‑1 (MQ=255)


                        aGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGt                                                                         >  2:4288317/1‑101 (MQ=255)


                         gCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGtt                                                                        >  1:3940138/1‑101 (MQ=255)


                          cACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGttt                                                                       <  2:2210019/101‑1 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTc                                                                      <  1:1773239/101‑1 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     >  2:2333704/1‑101 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     >  2:1827080/1‑101 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     <  2:2709816/101‑1 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     <  1:2135702/101‑1 (MQ=255)


                             cTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCaa                                                                    >  1:1352829/1‑101 (MQ=255)


                              tGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAc                                                                   <  1:3471372/101‑1 (MQ=255)


                              tGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAc                                                                   <  2:359996/101‑1 (MQ=255)


                              tGTTGCGGATCATCGACAAGATCCTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAc                                                                   <  1:3203195/101‑1 (MQ=255)


                                 tGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcgcg                                                                <  2:3664816/101‑1 (MQ=255)


                                  gCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGa                                                               >  2:2640928/1‑101 (MQ=255)


                                    ggATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATg                                                             >  1:169423/1‑101 (MQ=255)


                                     gATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGGTTCAAGGCGATgg                                                            >  1:4144535/1‑101 (MQ=255)


                                      atcatcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATggg                                                           >  2:1528009/1‑100 (MQ=255)


                                           cGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGcccc                                                      <  2:4203085/101‑1 (MQ=255)


                                            gACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGccccc                                                     <  1:1876083/101‑1 (MQ=255)


                                              cAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCgg                                                   <  1:1172265/101‑1 (MQ=255)


                                              cAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCgg                                                   <  2:4420272/101‑1 (MQ=255)


                                              cAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCgg                                                   <  1:759014/101‑1 (MQ=255)


                                              cAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGGGCCCCCgg                                                   >  2:1260740/1‑101 (MQ=255)


                                               aaGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGt                                                  >  1:2612908/1‑101 (MQ=255)


                                                aGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTc                                                 >  1:1967560/1‑101 (MQ=255)


                                                 gATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTcg                                                <  1:4067605/101‑1 (MQ=255)


                                                 gATCGTGTCCGCGGAGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGCCGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGAAAGCGCCCCCGGAcg                                                >  2:3401325/1‑101 (MQ=255)


                                                  aTCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTcgc                                               <  2:1692579/101‑1 (MQ=255)


                                                    cGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCgg                                             >  1:2221025/1‑101 (MQ=255)


                                                     gtgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCTCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGt                                            <  1:3758808/101‑1 (MQ=255)


                                                     gtgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGGGCCCCCGGTCGCGGt                                            >  1:4399042/1‑101 (MQ=255)


                                                      tgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTg                                           >  2:1028031/1‑101 (MQ=255)


                                                       gtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGGgg                                          >  2:3466637/1‑101 (MQ=255)


                                                         ccGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGAt                                        >  1:812887/1‑101 (MQ=255)


                                                                  ttccgatcttCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgctc                               <  1:1829448/92‑1 (MQ=255)


                                                                  ggcggcGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgctc                               <  2:539980/101‑1 (MQ=255)


                                                                  ggcggcGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgctc                               >  1:3988889/1‑101 (MQ=255)


                                                                   gcggcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGctcgctcg                              <  2:609412/101‑1 (MQ=255)


                                                                     ggcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtc                            >  2:1762571/1‑101 (MQ=255)


                                                                     ggcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtc                            >  2:4348305/1‑101 (MQ=255)


                                                                      gcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtct                           <  1:62380/101‑1 (MQ=255)


                                                                         tgtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGa                        >  2:886561/1‑101 (MQ=255)


                                                                         tgtCCATGAAAGCGACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGa                        <  2:1111547/101‑1 (MQ=255)


                                                                           tCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATc                      <  1:2635500/101‑1 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATcc                     <  1:2500158/101‑1 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATcc                     <  2:4301417/101‑1 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATcc                     >  1:2824096/1‑101 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGGTTCAACGCGATGGCGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATcc                     >  1:1378177/1‑101 (MQ=255)


                                                                             gATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCg                    <  1:3656108/101‑1 (MQ=255)


                                                                              aTGAAAGCAACCCCTATGTCGACGCCCGTGTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCgg                   >  1:2454177/1‑101 (MQ=255)


                                                                              aTGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCgg                   <  1:27501/101‑1 (MQ=255)


                                                                              aTGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCgg                   <  1:1193274/101‑1 (MQ=255)


                                                                               tGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGa                  <  2:3006003/101‑1 (MQ=255)


                                                                               tGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGa                  <  1:3867576/101‑1 (MQ=255)


                                                                                    gCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGCTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTc             >  1:1939388/1‑101 (MQ=255)


                                                                                     cAACCCCTATGTCGACGTCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCa            <  2:2300030/101‑1 (MQ=255)


                                                                                      aaCCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaa           >  2:1207223/1‑101 (MQ=255)


                                                                                       tccccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGCTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaag          <  1:3106407/100‑1 (MQ=255)


                                                                                        ccccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaaga         >  2:3106183/1‑101 (MQ=255)


                                                                                        ccccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaaga         <  1:1235849/101‑1 (MQ=255)


                                                                                        ccccTATGTCGACGCCCGTCTGGCCGATGGCTCGCCTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaaga         <  1:1033120/101‑1 (MQ=255)


                                                                                         cccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaagaa        >  1:1099433/1‑101 (MQ=255)


                                                                                          ccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCGGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaagaag       <  1:1086356/101‑1 (MQ=255)


                                                                                          ccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGTTGGCTCGCTCGTCTCGATCCGGAAATTCaagaag       >  2:3201728/1‑101 (MQ=255)


                                                                                          ccTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaagaag       <  2:1352732/101‑1 (MQ=255)


                                                                                           cTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAgg      <  1:3178124/101‑1 (MQ=255)


                                                                                              tGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACa   <  1:1809330/101‑1 (MQ=255)


                                                                                              tGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACa   <  2:1150175/101‑1 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  <  1:1430345/101‑1 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  <  1:1541084/101‑1 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  <  2:3533631/101‑1 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAACGACaa  >  2:679007/1‑101 (MQ=255)


CGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACAA  >  NC_014034/539645‑539840

Base quality scores:


ATCG

< 3 ≤


ATCG

< 19 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG