Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,548 A→G 10.8% F687L (TTT→CTT)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,5480A→G10.8% 44.4 0.0 ‑0.7195F687L (TTT→CTT) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (12/9):  ref base (98/76):  total (110/85)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.98e-01

Only 100 of 215 total aligned reads displayed.

TCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCG  >  NC_014034/681448‑681648
                                                                                                    |                                                                                                    
tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                                                                                      <  2:4280718/101‑1 (MQ=255)
 cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                                                                                     <  1:780467/101‑1 (MQ=255)
 cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                                                                                     >  2:175137/1‑101 (MQ=255)
  gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                                                                                    >  2:1917211/1‑101 (MQ=255)
   aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGcc                                                                                                   <  1:3509416/101‑1 (MQ=255)
   aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGcc                                                                                                   >  2:2289594/1‑101 (MQ=255)
     cgccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                                                                                                 >  1:2597334/1‑99 (MQ=2)
      gccgcGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcgcg                                                                                                >  2:1293045/1‑101 (MQ=255)
      gccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                                                                                                <  1:3082810/101‑4 (MQ=9)
        cgcgATCTGCGTGCGGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGa                                                                                              <  2:2525780/101‑1 (MQ=255)
         gcgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                                                                                             <  1:1917335/101‑1 (MQ=255)
         gcgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                                                                                             >  1:4170241/1‑101 (MQ=255)
           gATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                                                                           <  1:3220519/101‑1 (MQ=255)
            accTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCCTCGGTGCCGAGGCCCTTGATcgc                                                                                          >  1:147936/3‑101 (MQ=2)
            aTCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgc                                                                                          >  1:4219645/1‑101 (MQ=255)
            aTCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgc                                                                                          >  2:861525/1‑101 (MQ=255)
             tCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                                                                                         >  1:3190304/1‑101 (MQ=255)
              cTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATcgcat                                                                                        >  2:3641289/1‑99 (MQ=2)
              cTGCGGGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                                                                                        <  2:3287112/101‑1 (MQ=255)
               tGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccgc                                                                                       <  1:1186836/101‑1 (MQ=255)
                  gTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                                                                                    >  2:2308907/1‑101 (MQ=255)
                   tGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTg                                                                                   >  2:4161584/1‑101 (MQ=255)
                       ggTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa                                                                               <  2:3574863/101‑1 (MQ=255)
                       ggTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa                                                                               >  1:4339994/1‑101 (MQ=255)
                        gTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAg                                                                              >  1:2038975/1‑101 (MQ=255)
                        atctcggtgcCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAg                                                                              >  2:2030951/9‑101 (MQ=255)
                         tCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc                                                                             <  1:685771/101‑1 (MQ=255)
                         tCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc                                                                             <  1:4161094/101‑1 (MQ=255)
                           aGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc                                                                           >  2:2347894/1‑101 (MQ=255)
                             cgcgcCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccg                                                                         <  2:817906/101‑1 (MQ=255)
                              gcgcCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccgc                                                                        <  2:546586/101‑1 (MQ=255)
                                gcCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa                                                                      <  2:1567954/101‑1 (MQ=255)
                                 ccGATCTGCCCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAc                                                                     <  1:826334/101‑1 (MQ=255)
                                  cGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAcc                                                                    >  1:1226624/1‑101 (MQ=255)
                                      cTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCg                                                                <  2:940128/101‑1 (MQ=255)
                                      cTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGGCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCg                                                                <  2:1396715/101‑1 (MQ=255)
                                       tGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCCGCCCCGCCCCCGTCGc                                                               >  1:1046853/1‑101 (MQ=255)
                                       tGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGc                                                               <  2:3125059/101‑1 (MQ=255)
                                       tGCGCCGAGCCCAGACGCGCCGCCTGCCCCGTCGTCAGCTCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGc                                                               <  2:3688540/101‑1 (MQ=255)
                                         cgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCg                                                             >  2:4029198/1‑101 (MQ=255)
                                         cgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCg                                                             >  1:71686/1‑101 (MQ=255)
                                         cgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCg                                                             >  1:2621328/1‑101 (MQ=255)
                                          gcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGa                                                            <  2:3497503/101‑1 (MQ=255)
                                           ccGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGaa                                                           <  1:3567722/101‑1 (MQ=255)
                                            cGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAg                                                          <  1:2523802/101‑1 (MQ=255)
                                            cGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAg                                                          <  2:4169980/101‑1 (MQ=255)
                                            cGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCCCCGCAGTGCCCAGCGCCGCCACCGCCGCCgcac                                                          >  1:1727166/1‑98 (MQ=37)
                                             gAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGc                                                         >  1:3406452/1‑101 (MQ=255)
                                              aGCCCAGCCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc                                                        <  2:1664634/101‑1 (MQ=255)
                                              aGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCCCCGTCGCCGAAGca                                                        >  1:3551043/1‑100 (MQ=255)
                                              aGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc                                                        <  1:4058288/101‑1 (MQ=255)
                                                cccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAg                                                      <  1:2433432/101‑1 (MQ=255)
                                                 ccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgc                                                     <  1:2952995/101‑1 (MQ=255)
                                                 ccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgc                                                     <  1:227247/101‑1 (MQ=255)
                                                    gACGCGCCCCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgcc                                                  <  1:1077993/101‑1 (MQ=255)
                                                     aCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGGGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgccg                                                 >  2:716535/1‑101 (MQ=255)
                                                       gcgcCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCcgcg                                               >  1:1177141/1‑101 (MQ=255)
                                                       ccgccgcCTGCGCCGTCGTCAGCGCCCCGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgca                                               >  2:2096282/2‑100 (MQ=25)
                                                         gccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGAt                                             >  2:227697/1‑101 (MQ=255)
                                                         gccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGAt                                             >  2:558931/1‑101 (MQ=255)
                                                          ccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATc                                            >  1:3942465/1‑101 (MQ=255)
                                                           cgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgcaagct                                           <  2:952210/101‑6 (MQ=37)
                                                            gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTg                                          >  1:3640733/1‑101 (MQ=255)
                                                             ccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTgc                                         >  1:2004199/1‑101 (MQ=37)
                                                              cTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcg                                        <  2:2873621/101‑1 (MQ=255)
                                                              cTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTCCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcg                                        >  2:64881/1‑101 (MQ=255)
                                                               tGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcgc                                       >  1:2502967/1‑101 (MQ=255)
                                                                  gcCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGt                                    >  2:4378896/1‑101 (MQ=255)
                                                                   ccGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTg                                   >  2:3880636/1‑101 (MQ=255)
                                                                    cgtcgtCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGc                                  >  1:3031839/1‑101 (MQ=255)
                                                                    cgtcgtCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGc                                  >  2:486234/1‑101 (MQ=255)
                                                                     gtcgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCt                                 >  1:2840947/1‑101 (MQ=255)
                                                                      tcgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTc                                <  2:68885/101‑1 (MQ=255)
                                                                      tcgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTc                                <  1:2207369/101‑1 (MQ=255)
                                                                       cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCa                               >  1:3779551/1‑101 (MQ=255)
                                                                       cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCa                               <  2:4192471/101‑1 (MQ=255)
                                                                          cAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                            <  2:112489/101‑1 (MQ=255)
                                                                          cAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                            >  2:2131196/1‑101 (MQ=255)
                                                                           aGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcgc                           >  1:3332760/1‑101 (MQ=255)
                                                                            gcgcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgcc                          <  1:1318374/101‑1 (MQ=255)
                                                                               ccACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgcc                       <  1:4007466/101‑1 (MQ=255)
                                                                                   gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                   >  1:576900/1‑101 (MQ=255)
                                                                                   gATGTCATCGGTGCCAAAGCCGCGGAGCCCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTTCGCCGTGCTCAGCGCCGCCGCCt                   <  2:2519267/101‑1 (MQ=255)
                                                                                    aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTg                  <  2:1226531/101‑1 (MQ=255)
                                                                                    aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTg                  >  2:2582160/1‑101 (MQ=255)
                                                                                     tGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGc                 >  2:3958104/1‑101 (MQ=255)
                                                                                     tGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGAACCGCCCCCGTCGCCGAAGCCAGCGCCGCGCTCTCCGCCGTGCTCAGCGCCGCCGCCtcc                 >  1:2751457/1‑99 (MQ=38)
                                                                                      gTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCa                <  1:1784879/101‑1 (MQ=255)
                                                                                      gTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCa                >  2:1747965/1‑101 (MQ=255)
                                                                                        cATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAcc              >  2:1003088/1‑101 (MQ=255)
                                                                                           cGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAg           >  2:4356492/1‑101 (MQ=255)
                                                                                           cGGTCCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAg           <  2:2009030/101‑1 (MQ=255)
                                                                                            ggTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGc          >  2:1585657/1‑101 (MQ=255)
                                                                                            ggTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGc          <  2:3406215/101‑1 (MQ=255)
                                                                                            ggTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGc          >  2:776302/1‑101 (MQ=255)
                                                                                              tGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCa        >  2:2176252/1‑101 (MQ=255)
                                                                                                ccAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAg      >  2:27554/1‑101 (MQ=255)
                                                                                                ccAAAGCCGCGGATCCCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAg      <  2:4211899/101‑1 (MQ=255)
                                                                                                  aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc    >  1:3654916/1‑101 (MQ=255)
                                                                                                  aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc    >  1:185118/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCG  >  NC_014034/681448‑681648

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG