STRAIN: SE8R6

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breseq  version 0.22  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSE8R6_1_sequence.txt4,449,891449,438,991100.0%101.0 bases101 bases96.9%
errorsSE8R6_2_sequence.txt4,449,619449,411,519100.0%101.0 bases101 bases95.9%
total8,899,510898,850,510100.0%101.0 bases101 bases96.4%

Reference Sequence Information

seq idlengthfit meanfit dispersiondescription
coveragedistributionNC_0140343,738,958228.02.8Rhodobacter capsulatus SB 1003 chromosome, complete genome.
coveragedistributionNC_014035132,962132.71.9Rhodobacter capsulatus SB 1003 plasmid pRCB133, complete sequence.
total3,871,920

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

Junction Evidence

reference sequencepr(no read start)
NC_0140340.37180
NC_0140350.54998

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Polymorphism E-value cutoff2
Polymorphism frequency cutoffOFF
Polymorphism minimum coverage each strandOFF
Polymorphism homopolymer length cutoffOFF
Polymorphism bias cutoff0
Polymorphism predict indel polymorphismsOFF

Execution Times

stepstartendelapsed
Read and reference sequence file input16:05:34 21 Mar 201316:09:18 21 Mar 20133 minutes 44 seconds
Read alignment to reference genome16:09:19 21 Mar 201316:50:09 21 Mar 201340 minutes 50 seconds
Preprocessing alignments for candidate junction identification16:50:09 21 Mar 201316:57:53 21 Mar 20137 minutes 44 seconds
Preliminary analysis of coverage distribution16:57:54 21 Mar 201317:07:59 21 Mar 201310 minutes 5 seconds
Identifying junction candidates17:07:59 21 Mar 201317:08:09 21 Mar 201310 seconds
Re-alignment to junction candidates17:08:09 21 Mar 201317:21:04 21 Mar 201312 minutes 55 seconds
Resolving alignments with junction candidates17:21:04 21 Mar 201317:32:41 21 Mar 201311 minutes 37 seconds
Creating BAM files17:32:41 21 Mar 201317:41:35 21 Mar 20138 minutes 54 seconds
Tabulating error counts17:41:35 21 Mar 201317:44:50 21 Mar 20133 minutes 15 seconds
Re-calibrating base error rates17:44:50 21 Mar 201317:44:54 21 Mar 20134 seconds
Examining read alignment evidence17:44:54 21 Mar 201318:19:13 21 Mar 201334 minutes 19 seconds
Polymorphism statistics18:19:13 21 Mar 201318:19:24 21 Mar 201311 seconds
Output18:19:24 21 Mar 201318:36:39 21 Mar 201317 minutes 15 seconds
Total 2 hours 31 minutes 3 seconds