Rv0377 Transcription regulator in CO-DH cluster


Product Feature Type Start End Strand Length AA Length is TF
Rv0377 Transcription regulator in CO-DH cluster CDS 454421 455386 + 966 321 TRUE

Rv0377 (Transcription regulator in CO-DH cluster) is predicted to be co-regulated in modules bicluster_0066 with residual 0.56 and bicluster_0467 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 47.00 and 3,300.00 for bicluster_0066 and 0.00 and 0.04 for bicluster_0467 respectively.

These modules are enriched for following go terms: glycerol ether metabolic process, ether metabolic process, protein disulfide oxidoreductase activit..., disulfide oxidoreductase activity generation of precursor metabolites and ..., oxidation-reduction process, single-organism metabolic process, deaminase activity, hydrolase activity, acting on carbon-nit....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized HTH-type transcriptional regulator Rv0377_MT0391 LysR family transcriptional regulator
Operon # Operon
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15607518 NP_214891.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426474 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426475 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426476 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv0377_B414 UCSC Browser Tracks

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.150000 3.76

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.909512
p-value INH: 0.988908
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 7.45 U
D3I 3 3 3.83 7.17 I
D3U 3 3 3.83 6.31 U
D5I 9 5 6.00 10.18 I
D5U 17 5 6.00 9.97 U
D7I 18 7 8.14 7.24 I
D7U 19 7 8.14 7.00 U
D14U 4 14 15.63 6.33 U
D14I 4 14 15.63 5.33 I
D17I 3 17 19.15 4.25 I
D17U 3 17 19.15 6.15 U
D21I 4 21 23.23 5.31 I
D21U 4 21 23.23 6.37 U
D24I 3 24 26.60 4.43 I
D24U 3 24 26.60 6.32 U
D28I 4 28 30.61 3.58 I
D28U 4 28 30.61 6.08 U