Rv1358 Adenylyl cyclase class-3/4/guanylyl cyclase / Disease resistance domain-containing protein / Tetratricopeptide repeat-containing protein / Transcriptional regulator, LuxR family

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv1358 Adenylyl cyclase class-3/4/guanylyl cyclase / Disease resistance domain-containing protein /... CDS 1526612 1530091 + 3 480 1 159 FALSE

Rv1358 (Adenylyl cyclase class-3/4/guanylyl cyclase / Disease resistance domain-containing protein / Tetratricopeptide repeat-containing protein / Transcriptional regulator, LuxR family) is predicted to be co-regulated in modules bicluster_0467 with residual 0.48 and bicluster_0563 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.04 for bicluster_0467 and 0.00 and 1,400.00 for bicluster_0563 respectively.

These modules are enriched for following go terms: generation of precursor metabolites and ..., oxidation-reduction process, single-organism metabolic process, deaminase activity, hydrolase activity, acting on carbon-nit... .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.768 1526717 1526612
Product (LegacyBRC) Product (RefSeq)
PROBABLE TRANSCRIPTIONAL REGULATORY PROTEIN transcriptional regulatory protein
Operon # Operon
912
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608498 NP_215874.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.140000 1.33

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.391485
p-value INH: 0.844561
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D24I 3 24 26.60 5.46 I
D28I 4 28 30.61 5.98 I
D21I 4 21 23.23 7.19 I
D17I 3 17 19.15 7.58 I
D14I 4 14 15.63 8.57 I
D24U 3 24 26.60 8.65 U
D17U 3 17 19.15 9.02 U
D21U 4 21 23.23 9.07 U
D28U 4 28 30.61 9.14 U
D14U 4 14 15.63 9.63 U
D3U 3 3 3.83 11.27 U
D3I 3 3 3.83 11.30 I
D7U 19 7 8.14 11.59 U
D7I 18 7 8.14 12.01 I
D0U 27 0 0.00 12.07 U
D5I 9 5 6.00 14.50 I
D5U 17 5 6.00 14.53 U