Rv3025c Cysteine desulfurase (EC 2.8.1.7)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3025c iscS Cysteine desulfurase (EC 2.8.1.7) CDS 3383885 3385066 - 1 182 393 FALSE

Rv3025c (Cysteine desulfurase (EC 2.8.1.7)) is predicted to be co-regulated in modules bicluster_0006 with residual 0.61 and bicluster_0321 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 8.50 and 1,100.00 for bicluster_0006 and 380.00 and 15,000.00 for bicluster_0321 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE CYSTEINE DESULFURASE ISCS [NIFS PROTEIN HOMOLOG] [NITROGENASE METALLOCLUSTERS BIOSYNTHESIS PROTEIN NIFS] cysteine desulfurase IscS
Operon # Operon
1980 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Cysteine desulfurase Thiamine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Thiamine metabolism

9
Total items in this category:  

KEGG

Sulfur relay system

18
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610162 NP_217541.1 Run
GO:0031071

cysteine desulfurase activity

cysteine desulfurase activity

Details: 
Catalysis of the reaction: L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0016226

iron-sulfur cluster assembly

iron-sulfur cluster assembly

Details: 
The incorporation of iron and exogenous sulfur into a metallo-sulfur cluster.
GO Category: 
biological_process
1
Total items in this category:  
GO:0031071

cysteine desulfurase activity

cysteine desulfurase activity

Details: 
Catalysis of the reaction: L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: