Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,158 Δ1 bp 5.4% coding (40/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1580C→. 5.4% 20.8 ‑0.7 ‑0.3167coding (40/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/6):  ref base (76/82):  total (79/88)
Fisher's exact test strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.54e-01

Only 100 of 222 total aligned reads displayed.

CGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396058‑1396258
                                                                                                      |                                                                                                     
cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                       <  2:4620435/101‑1 (MQ=255)
cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGcgccgc                                                                                                       >  2:344575/1‑101 (MQ=255)
cGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCcgccgc                                                                                                       >  1:3294525/1‑101 (MQ=255)
 gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccgcc                                                                                                      <  1:2079925/101‑1 (MQ=255)
 gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCgccccc                                                                                                      >  2:1068643/1‑101 (MQ=255)
 gAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCCCgccccc                                                                                                      >  1:1361085/1‑101 (MQ=255)
  aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCa                                                                                                     <  2:371462/101‑1 (MQ=255)
  aaaCAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTTTTCGTC.ATCTGG.CGGGCTCGCGCCGCCa                                                                                                     >  1:39839/1‑101 (MQ=255)
      aGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCt                                                                                                 <  2:4570156/101‑1 (MQ=255)
      aGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCCCCACGcc                                                                                                 >  1:4356455/1‑100 (MQ=255)
        gcgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGc                                                                                               >  2:745756/1‑101 (MQ=255)
          gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGGCACGCCGCgg                                                                                             >  2:1977547/1‑100 (MQ=255)
          gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGt                                                                                             >  2:3408897/1‑101 (MQ=255)
           cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTc                                                                                            <  1:3430976/101‑1 (MQ=255)
            aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTcc                                                                                           >  1:537422/1‑100 (MQ=255)
            aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCg                                                                                           >  1:4125989/1‑101 (MQ=255)
             tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGc                                                                                          <  1:1977664/101‑1 (MQ=255)
              cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCt                                                                                         <  1:1703059/101‑1 (MQ=255)
              cTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCCGCGTCGCt                                                                                         >  1:2969149/1‑101 (MQ=255)
               tGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTc                                                                                        >  2:2402785/1‑101 (MQ=255)
                gATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTcc                                                                                       >  2:3043371/1‑101 (MQ=255)
                 aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTccc                                                                                      >  2:886399/1‑101 (MQ=255)
                 aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccc                                                                                      >  1:652836/1‑101 (MQ=255)
                 aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACCCTGCGTCGCTccc                                                                                      >  1:2588816/1‑101 (MQ=255)
                   gTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCCCTccccc                                                                                    >  2:1418765/1‑101 (MQ=255)
                    tataTGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTcccccc                                                                                   >  1:91800/1‑101 (MQ=255)
                     atatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccccc                                                                                  >  1:1824298/1‑100 (MQ=255)
                     atatGCCCGGTGAGACGGCTGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTccccccc                                                                                  >  1:2694517/1‑100 (MQ=255)
                        tGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCgg                                                                               <  2:1295602/101‑1 (MQ=255)
                            cGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGc                                                                           >  2:2875943/1‑101 (MQ=255)
                              gTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGCC.ATCTGG.CAGGCTCCCGCCGCACCGCCGCGCCTCTCCCCCCCCgggggggg                                                                         >  1:4708137/1‑98 (MQ=25)
                               tgtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                        >  2:1886555/11‑101 (MQ=9)
                               tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCggg                                                                        >  1:3656463/1‑99 (MQ=255)
                               tGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTg                                                                        <  2:4230507/101‑1 (MQ=255)
                                gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                       <  2:4033645/92‑1 (MQ=2)
                                gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                       <  1:983142/92‑1 (MQ=2)
                                gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCGCTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                       <  1:2742549/92‑1 (MQ=2)
                                  gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                     <  2:1575601/101‑1 (MQ=255)
                                  gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                     <  1:279901/101‑1 (MQ=255)
                                  ctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCCGCCTCGATCCGGTCAGCCACGTATTG.CGCGCTG.GTCGCTCACCCCGCGGGGGCGTGGAt                                                                     >  2:1545657/8‑101 (MQ=2)
                                    cGGCTGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   <  1:3490663/101‑1 (MQ=255)
                                        tGTTGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCG.AAAc                                                              >  1:3129290/1‑101 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAAcc                                                             >  2:4125533/1‑101 (MQ=255)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCCCGGGGGCGTGGATCG.AAAcc                                                             >  1:4404526/1‑101 (MQ=255)
                                         gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAAcc                                                             <  1:1073624/101‑1 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCgg                                                          >  1:3168384/1‑101 (MQ=255)
                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTGTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCgg                                                          >  1:4222408/1‑101 (MQ=255)
                                               ccccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGa                                                       <  2:2735335/101‑1 (MQ=255)
                                                cccccGCAGGGTCGTGGATCGAACCTTTTCGTC.ATCTGG.CAGCCTGGCGCCAACCCGCTGCGTCCCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaa                                                      <  2:297817/101‑1 (MQ=25)
                                                 ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaa                                                     <  2:2636561/101‑1 (MQ=255)
                                                 ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGaaa                                                     <  1:1159199/101‑1 (MQ=255)
                                                       gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGc                                               >  2:2153645/1‑101 (MQ=255)
                                                       gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGGGGATCG.AAACCCGGCGAAAAACGGc                                               >  2:1952544/1‑101 (MQ=255)
                                                         gggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCa                                             <  2:3804653/101‑1 (MQ=255)
                                                          ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCAt                                            <  1:272742/101‑1 (MQ=255)
                                                          ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCAt                                            >  2:3825698/1‑101 (MQ=255)
                                                           gCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATg                                           <  2:3894336/101‑1 (MQ=255)
                                                           gCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATg                                           <  1:1068722/101‑1 (MQ=255)
                                                            cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGc                                          >  1:802627/1‑101 (MQ=255)
                                                             gTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAAAGGCCATGcc                                         >  1:2943322/1‑101 (MQ=255)
                                                              tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCt                                        <  1:172893/101‑1 (MQ=255)
                                                              tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCt                                        >  2:1622606/1‑101 (MQ=255)
                                                              tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGACGCCAAGTTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGccc                                        >  1:1640593/1‑100 (MQ=255)
                                                               ngNNCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTc                                       <  1:2957933/100‑1 (MQ=255)
                                                               ggATCGAAACTTTTCGTC.ATCTGG.CAGGCCCGCGCCCCCCCGCCTGGGCCCTCCCCCCCCGGGGGCGGGGGTCGAAAACCCGGGGAAAAACGGcacggccc                                        >  2:3356747/1‑94 (MQ=255)
                                                                gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTcc                                      <  1:661565/101‑1 (MQ=255)
                                                                 aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCg                                     <  1:4071441/101‑1 (MQ=255)
                                                                 aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCg                                     <  2:2437921/101‑1 (MQ=255)
                                                                  tCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCgg                                    >  2:289567/1‑101 (MQ=255)
                                                                  tCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCgg                                    <  1:2655209/101‑1 (MQ=255)
                                                                   cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCggg                                   >  2:2259782/1‑101 (MQ=255)
                                                                   cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCggg                                   >  1:4637567/1‑101 (MQ=255)
                                                                    gAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGgt                                  <  2:3204444/101‑1 (MQ=255)
                                                                       aCTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTc                               <  2:2060619/101‑1 (MQ=255)
                                                                        cTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCt                              <  1:495618/101‑1 (MQ=255)
                                                                           ttCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGc                           >  2:3365259/1‑101 (MQ=255)
                                                                             cGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTg                         >  1:4448034/1‑101 (MQ=255)
                                                                              atctaTCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTg                         <  1:3388508/97‑1 (MQ=255)
                                                                               tC.ATCTGG.CAGGCTCGCGCCGCCCCGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                       <  1:4715882/101‑1 (MQ=255)
                                                                               tC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                       >  2:476572/1‑101 (MQ=255)
                                                                               tC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                       <  1:3328938/101‑1 (MQ=255)
                                                                               tC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                       <  2:1775601/101‑1 (MQ=255)
                                                                               tC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTc                       <  2:2209958/101‑1 (MQ=255)
                                                                               tC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTGTc                       >  1:1509109/1‑101 (MQ=255)
                                                                                c.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                      <  2:443141/101‑1 (MQ=255)
                                                                                  aTCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGc                     >  2:3632692/1‑101 (MQ=255)
                                                                                    cTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTc                   <  2:4057628/101‑1 (MQ=255)
                                                                                     tGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc                  >  2:3866857/1‑101 (MQ=255)
                                                                                     tGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCGGTCGCTcc                  >  1:1881722/1‑101 (MQ=255)
                                                                                       g.CAGGCCCGCCCCGCCGCGGTGGCTGCCCCCCCCCGGGGGGGCGTGGATTG.AAACCCGGCGAAAAACCGGCAATCCCCCGGGTGTTCTGCTGTCGCCcccc                <  2:859217/101‑1 (MQ=255)
                                                                                         cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc               <  1:1311222/101‑1 (MQ=255)
                                                                                         cAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccccc               >  1:4031508/1‑101 (MQ=255)
                                                                                          aGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc              <  1:2203485/101‑1 (MQ=255)
                                                                                           ggCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCcg             <  2:3288951/101‑1 (MQ=255)
                                                                                                gcgcCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCgggg        >  1:3145696/1‑101 (MQ=255)
                                                                                                   ccgccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCg     >  1:4283292/1‑101 (MQ=255)
                                                                                                      ccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTgg  >  2:4483557/1‑101 (MQ=255)
                                                                                                      ccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTgg  <  1:750425/101‑1 (MQ=255)
                                                                                                      ccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTgg  >  2:807248/1‑101 (MQ=255)
                                                                                                      ccACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTgg  >  2:1162159/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
CGAAACAGGCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACGCTGCGTCGCTCCCCCCGCGGGGGCGTGGATCG.AAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1396058‑1396258

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG