Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,607,903 Δ1 bp 100% intergenic (+173/‑174) RCAP_rcc01484 → / → murD hypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,607,9030G→.100.0% 15.0 118intergenic (+173/‑174)RCAP_rcc01484/murDhypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  new base (35/75):  ref base (5/2):  total (41/77)
Fisher's exact test strand distribution p-value = 4.52e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.45e-05

Only 100 of 156 total aligned reads displayed.

GCCTTGCGGGCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAG  >  NC_014034/1607803‑1608003
                                                                                                    |                                                                                                    
gCCTTGCGGGCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCg                                                                                                      <  1:3624159/101‑1 (MQ=255)
     gCGGGCGCCGTATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCgggaat                                                                                                 <  1:687682/101‑4 (MQ=255)
       gggCGCCGGATCGGGGCGGACGCCGCCCGGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGc                                                                                              <  2:4257360/101‑1 (MQ=255)
        ggCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGaagcg                                                                                              >  2:3330121/1‑98 (MQ=255)
        ggCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCg                                                                                             <  2:368905/101‑1 (MQ=255)
         gcgcCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGTCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCgg                                                                                            <  2:4205167/101‑1 (MQ=255)
          cgcCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGGCGCCCCGGG.ATGCggg                                                                                           >  2:2613120/1‑101 (MQ=255)
          cgcCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCggg                                                                                           <  2:1896334/101‑1 (MQ=255)
           gcCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGc                                                                                          <  2:2478256/101‑1 (MQ=255)
           gcCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGc                                                                                          <  2:3086792/101‑1 (MQ=255)
           gcCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGc                                                                                          <  2:4254878/101‑1 (MQ=255)
             cGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGcag                                                                                        <  2:146803/101‑3 (MQ=255)
              ggATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGcaga                                                                                       <  1:1240054/101‑4 (MQ=255)
                  cGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCgg                                                                                  >  2:329813/1‑101 (MQ=255)
                  cGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGG.GAGACCgg                                                                                  >  1:2231859/1‑101 (MQ=255)
                  cGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCgg                                                                                  <  2:1277508/101‑1 (MQ=255)
                  cGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCgg                                                                                  >  1:1485121/1‑101 (MQ=255)
                   ggggCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCggg                                                                                 >  1:2351304/1‑101 (MQ=255)
                   ggggCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.CGGAATGCGGGC.CGACCggg                                                                                 >  1:4439467/1‑101 (MQ=255)
                      gCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGG.GAGACCGGGCCg                                                                              >  2:37158/1‑101 (MQ=255)
                       cTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCga                                                                             <  1:2991246/101‑2 (MQ=255)
                        tGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGGGGGCC.GGGAAAGCGGG.GAGACCggggggga                                                                            >  2:2847218/1‑96 (MQ=255)
                        tGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGGCC.GGGAATGCGGGC.AGACCGGGCCgac                                                                            >  2:4332956/1‑99 (MQ=255)
                        tGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAACGCGGGC.AGACCGGGCCgac                                                                            >  1:2213847/1‑99 (MQ=255)
                         gACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCCGGG.ATGCGGGC.AGACCGGGCCgacc                                                                           >  1:4179873/1‑98 (MQ=255)
                         gACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCgacc                                                                           <  1:2808284/101‑4 (MQ=255)
                            gccgccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCCGCCCCGGG.ATGCGGGC.AGACCGGGCCgacccgc                                                                        >  2:612442/1‑95 (MQ=255)
                             ccgccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCgaccgccg                                                                       <  1:2079437/101‑8 (MQ=255)
                             ccgccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCCgaccgccc                                                                       >  1:2331671/1‑94 (MQ=255)
                               gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                    >  1:10013/1‑101 (MQ=255)
                                  aCGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCGCCCGt                                                                 >  1:4616334/1‑101 (MQ=255)
                                  aCGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGt                                                                 <  1:4587834/101‑1 (MQ=255)
                                  aCGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAAAGCGGGC.CGACCGGGGC.GACCGCCGCCTgc                                                                 >  1:4365869/1‑100 (MQ=255)
                                   cGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTc                                                                <  2:2343067/101‑1 (MQ=255)
                                     cccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtc                                                              >  1:3559860/1‑100 (MQ=255)
                                     cccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtc                                                              <  2:2325916/101‑2 (MQ=255)
                                     cccGCATTGGGCCCGCCGCGCCCCAGAACGCGCGCCCAGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtc                                                              <  1:4008234/101‑2 (MQ=255)
                                      ccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtcc                                                             <  1:1854767/101‑3 (MQ=255)
                                       cGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccg                                                            <  2:4614988/101‑4 (MQ=255)
                                          cttGTGCCCGCGGCGCCCCAGAACGGCCGCCCCGGCCCCTCAAGGATGGCGGC.GCCCCGGGAAGGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAcc                                                        <  2:2209586/100‑1 (MQ=255)
                                          aTTGTGGCTGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGGGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCCCCTGGCTCCCgcc                                                         >  2:2957928/1‑99 (MQ=255)
                                          aTTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAcc                                                        >  2:1497527/1‑101 (MQ=255)
                                            tgtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGACCtt                                                      >  1:4296006/1‑101 (MQ=255)
                                            tggggCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtt                                                      <  1:2893986/98‑1 (MQ=255)
                                             gtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATTGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCCCCCGTC.CCCGACCttt                                                     >  2:2972441/1‑101 (MQ=255)
                                             gtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCttt                                                     <  2:3447351/101‑1 (MQ=255)
                                             gtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCttt                                                     <  1:1203393/101‑1 (MQ=255)
                                                 ccgccgCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGTCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                 <  1:767612/101‑1 (MQ=255)
                                                  cgccgcGCCCCAGACCGGGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GCGAATGCGGGC.AGACCGGG.CGGACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                <  2:4748810/101‑1 (MQ=255)
                                                  cgccgcGCCCCAGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                <  2:944091/101‑1 (MQ=255)
                                                  cgccgcGCCCCAGAACGCGCGCCCCGGCCGCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGCG.GACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                <  2:4365622/101‑1 (MQ=255)
                                                    ccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCCGc                                              >  1:266805/1‑101 (MQ=255)
                                                      gcgcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCgg                                            <  2:428017/101‑1 (MQ=255)
                                                      gcgcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.CACCGCCGCCCGTC.TCCGACCTTTTCCCAGCgg                                            >  2:394846/1‑101 (MQ=255)
                                                          cccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGGCTGTC.TCCGACCCTTTCCCACCGGATcc                                        >  2:1372450/1‑101 (MQ=255)
                                                          cccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATcc                                        <  2:3920880/101‑1 (MQ=255)
                                                          cccAGAACGCGCGCCCCAGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATcc                                        >  1:1720973/1‑101 (MQ=255)
                                                           ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                       <  1:1985277/101‑1 (MQ=255)
                                                           ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                       <  2:2339750/101‑1 (MQ=255)
                                                            cAGAACGCGCCCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                      <  2:3559593/101‑1 (MQ=255)
                                                             aGACCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGG.CCGGGGAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                     <  1:355772/101‑1 (MQ=255)
                                                              gAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                    <  1:2977463/101‑1 (MQ=255)
                                                              gAACGCGCGCCCCGGCCCCCCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcg                                    <  1:3967457/101‑1 (MQ=255)
                                                               aaCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgcgc                                   >  2:4506993/1‑100 (MQ=255)
                                                               aaCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGa                                   <  1:478409/101‑1 (MQ=255)
                                                                   cgcgcCCCGGCCCCTCAAGGATGGCGGGGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTACCCGCGGATCCCGCGACggg                               >  2:2458509/1‑101 (MQ=255)
                                                                   cgcgcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGACCTTTTCCCAGCGGATCCCCCGCCggg                               >  2:1406761/1‑101 (MQ=255)
                                                                    gcgcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACgggg                              <  1:2971786/101‑1 (MQ=255)
                                                                       ccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGc                           >  2:4010409/1‑101 (MQ=255)
                                                                        cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                          <  1:4546495/101‑1 (MQ=255)
                                                                        cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                          <  2:3081566/101‑1 (MQ=255)
                                                                        cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                          >  1:3574203/1‑101 (MQ=255)
                                                                        cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                          <  1:619951/101‑1 (MQ=255)
                                                                        cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCg                          >  1:4047385/1‑101 (MQ=255)
                                                                         ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGa                         <  2:3729763/101‑1 (MQ=255)
                                                                         ccGCCCCCTCAGGGAGGGGGGC.GCCCGGGGAAGGCGGGC.AGCCCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGa                         <  1:3246064/101‑1 (MQ=255)
                                                                          cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGAc                        <  2:2163813/101‑1 (MQ=255)
                                                                           ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACg                       >  2:12129/1‑101 (MQ=255)
                                                                            gCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACGc                      >  2:1270787/1‑101 (MQ=255)
                                                                             ccccTCAAGGAGGGGGGCGGCGC.GGGAATGGGGGC.ACCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGcc                     <  1:1666080/101‑1 (MQ=255)
                                                                               ccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACGCCtg                   >  1:1218877/1‑100 (MQ=255)
                                                                                cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                  <  1:4668817/101‑3 (MQ=255)
                                                                                cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                  <  2:2384346/101‑3 (MQ=255)
                                                                                 tCAGGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                 <  2:874111/101‑4 (MQ=255)
                                                                                 tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGcagatc                 >  1:397666/1‑96 (MQ=255)
                                                                                 tCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                 >  1:1938771/1‑98 (MQ=255)
                                                                                  cAAGGATGGCGGC.GCCCCGGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGGGGCCtgcgg                >  2:1172845/1‑97 (MQ=255)
                                                                                   aaGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggc              >  2:54916/1‑101 (MQ=255)
                                                                                    aGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.Tgcggcg             >  1:610679/1‑101 (MQ=255)
                                                                                     ggATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGACGCC.TGCGgcgc            >  1:1673106/1‑101 (MQ=255)
                                                                                      gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc           <  1:1456730/101‑1 (MQ=255)
                                                                                       aTGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGGC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGAGCGACGccctcggccccg           >  2:557788/1‑91 (MQ=255)
                                                                                           cggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg      <  2:4295759/101‑1 (MQ=255)
                                                                                           cggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg      >  2:1067020/1‑101 (MQ=255)
                                                                                           cggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg      <  1:4302510/101‑1 (MQ=255)
                                                                                            ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGa     >  2:1583132/1‑101 (MQ=255)
                                                                                            ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGa     <  2:4736526/101‑1 (MQ=255)
                                                                                              gcggccGGGGAATGCGGGC.AGACGGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaa    <  2:4113781/97‑1 (MQ=255)
                                                                                                ggccGGGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg  <  2:4439217/99‑1 (MQ=255)
                                                                                                gcCCCGGGAATGCGGG.GAGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAg  <  1:3199277/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GCCTTGCGGGCGCCGGATCGGGGCTGACGCCGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAG  >  NC_014034/1607803‑1608003

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG