Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 1,696,206 | Δ1 bp | 98.4% | intergenic (‑134/+91) | RCAP_rcc01564 ← / ← mmsA | isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase |
Read alignment evidence... | ||||||||||
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seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 1,696,206 | 0 | G→. | 98.4% | 2.2 ‑2.3 ‑1.8 | 192 | intergenic (‑134/+91) | RCAP_rcc01564/mmsA | isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase |
Reads supporting (aligned to +/- strand): new base (87/102): ref base (3/0): total (90/102) | ||||||||||
Fisher's exact test strand distribution p-value = 1.01e-01 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.53e-02 |
Only 100 of 194 total aligned reads displayed.
AATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAG > NC_014034/1696110‑1696300
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tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca < 2:4692694/101‑2 (MQ=255)
ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac < 2:3067531/101‑3 (MQ=255)
tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATACACCCCGGGACGTTTGGGCCAA..GGTGAAacacc > 1:2672944/1‑98 (MQ=255)
gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGGCAA..GGTGAAacacctt > 2:1759935/1‑96 (MQ=12)
gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt < 1:782048/101‑6 (MQ=255)
gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaactt > 2:4582449/1‑99 (MQ=12)
aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg > 2:4522496/1‑95 (MQ=255)
ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt > 2:3613016/1‑92 (MQ=255)
ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt < 1:4234604/101‑10 (MQ=9)
ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt > 1:2591201/1‑92 (MQ=255)
gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc < 2:2563312/101‑1 (MQ=255)
gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc > 2:1797634/1‑101 (MQ=255)
cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt < 2:1157285/101‑1 (MQ=255)
aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt < 2:710272/101‑1 (MQ=9)
aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt < 1:1057439/101‑1 (MQ=9)
cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg < 1:1365413/101‑1 (MQ=255)
cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg > 2:3849871/1‑101 (MQ=255)
cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg < 1:3842979/101‑1 (MQ=255)
cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg < 2:4117171/101‑1 (MQ=255)
cGAATTTTGCAAGGAGGCGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg < 2:4266530/101‑1 (MQ=255)
gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..GCCTTGCTTTTTCTTgg < 1:3614949/101‑1 (MQ=255)
gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg < 2:509463/101‑1 (MQ=255)
aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc > 1:4688075/1‑101 (MQ=255)
aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc < 2:3948760/101‑1 (MQ=9)
aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg < 2:1926297/101‑1 (MQ=255)
ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc > 2:2030324/1‑101 (MQ=9)
tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa > 1:2371884/1‑101 (MQ=255)
tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa > 1:1940386/1‑101 (MQ=255)
tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa > 1:4128518/1‑101 (MQ=9)
tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa > 1:2061442/1‑101 (MQ=9)
gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt > 1:4199260/1‑101 (MQ=255)
gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTG.TTTCA.CCTTGCTTTTTCTTGGCGCAAAt < 2:3028697/101‑1 (MQ=2)
cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa < 1:2808289/101‑1 (MQ=255)
aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATAc < 1:4300157/101‑1 (MQ=255)
aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc < 2:3106469/101‑1 (MQ=255)
aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc > 1:712446/1‑101 (MQ=9)
aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg < 2:4439867/101‑1 (MQ=9)
aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct < 2:1293761/101‑1 (MQ=9)
gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct < 2:4372683/101‑1 (MQ=255)
gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct < 1:4361545/101‑1 (MQ=255)
gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct > 1:1118131/1‑101 (MQ=9)
gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc > 2:4559569/10‑101 (MQ=16)
aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc > 1:3169384/9‑101 (MQ=16)
ggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg < 1:1123222/94‑1 (MQ=255)
ggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg > 2:2333317/8‑101 (MQ=12)
gggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa > 1:1666108/7‑101 (MQ=16)
ggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa > 2:4194955/6‑101 (MQ=16)
gcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg < 1:1582882/97‑1 (MQ=255)
cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg > 2:3676459/4‑101 (MQ=16)
ctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc < 1:3968355/100‑1 (MQ=19)
ctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc > 1:2624254/2‑101 (MQ=16)
ctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc < 1:3661463/100‑1 (MQ=255)
ctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc < 2:3821435/100‑1 (MQ=255)
ttccgatcttGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa < 1:3562819/92‑1 (MQ=16)
tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa < 1:1342061/101‑1 (MQ=19)
tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa > 1:4184023/1‑101 (MQ=16)
gCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa < 2:2964375/101‑1 (MQ=255)
gCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa < 1:421007/101‑1 (MQ=255)
gCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCC.AAGGG.GAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa > 1:3388995/1‑101 (MQ=19)
ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg < 1:2999592/101‑1 (MQ=255)
ccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc > 1:171891/1‑101 (MQ=19)
cTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg < 1:4029361/101‑1 (MQ=19)
cTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg < 1:2242794/101‑1 (MQ=255)
ctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgcgc < 2:142872/100‑1 (MQ=19)
ctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgcgc > 2:2593146/2‑101 (MQ=255)
ggCCTTTCGGCCAATTCGCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg < 1:2325558/101‑1 (MQ=19)
ggCCTTTCGGCCAATTCACCCCNGGANNNTNGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg < 1:2708938/101‑1 (MQ=255)
ccTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa < 1:1923404/101‑1 (MQ=19)
ccTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa < 1:179516/101‑1 (MQ=19)
cTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg < 2:2139435/101‑1 (MQ=19)
cTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg > 2:2162204/1‑101 (MQ=255)
tttCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAgg > 2:1241919/1‑101 (MQ=255)
tcggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg > 2:2583068/6‑101 (MQ=255)
ggccAATTCACCCCGGGATGTTTGGGCCAA..GGCGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg > 1:1643640/4‑101 (MQ=19)
gcaattcgcccGGGGATGTTTGGGCAAA.GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGc < 2:1836203/93‑1 (MQ=255)
aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc > 1:649195/1‑101 (MQ=25)
aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc > 1:4413879/1‑101 (MQ=255)
aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc < 2:737674/101‑1 (MQ=25)
attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc > 1:3264214/7‑101 (MQ=255)
ttcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CATTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGCAGGCCCCGccccc > 2:2468519/6‑101 (MQ=19)
caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt > 2:349396/4‑101 (MQ=255)
cccccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg < 2:1875194/99‑1 (MQ=25)
cccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg < 2:1344501/99‑1 (MQ=37)
ccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGa > 2:270900/2‑101 (MQ=255)
ccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGa < 2:4670131/100‑1 (MQ=37)
cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt > 1:1102114/1‑101 (MQ=255)
ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc > 1:880304/1‑101 (MQ=255)
ggatgtttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTg < 2:1102028/95‑1 (MQ=37)
ggatgtttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAACTACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGAGCGGCCCCCCTGATCGTg > 2:3230932/7‑101 (MQ=255)
gggtgTTTGGGCAAA.GGTGAAACC.CCTTGG.TTTTTCTGGGCGCAAATGCGCTTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTg < 1:4614699/97‑1 (MQ=255)
ggaGGTTTGGGCAAA.GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTg < 1:1231192/99‑1 (MQ=255)
gatgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGc > 2:2770782/6‑101 (MQ=255)
atgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGcc > 2:4144068/5‑101 (MQ=255)
atgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGcc > 2:3404605/5‑101 (MQ=255)
tgtttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCt > 2:4222012/4‑101 (MQ=37)
tttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTGGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa < 2:2898267/100‑1 (MQ=255)
ttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAg > 1:4398642/1‑101 (MQ=255)
ttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAg > 2:169454/1‑101 (MQ=255)
ttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAg > 1:4135601/1‑101 (MQ=255)
ttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAg < 2:1161192/101‑1 (MQ=255)
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AATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAG > NC_014034/1696110‑1696300
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 18 ≤
ATCG
< 27 ≤
ATCG
< 33 ≤
ATCG
< 41 ≤
ATCG
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