Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,796,987 Δ1 bp 100% coding (11/2238 nt) RCAP_rcc01660 → signal transduction histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,796,9870G→.100.0% 984.5 223coding (11/2238 nt)RCAP_rcc01660signal transduction histidine kinase
Reads supporting (aligned to +/- strand):  new base (118/104):  ref base (0/1):  total (118/105)

Only 100 of 244 total aligned reads displayed.

ACCTATGGCGAGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCAGGACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCATGCCGAGGA  >  NC_014034/1796887‑1797083
                                                                                                    |                                                                                                
aCCTATGGCGAGCAGGTGGGGGCGATTCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCAg                                                                                                  <  1:2856158/101‑1 (MQ=255)
aCCTATGGCGAGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCAg                                                                                                  >  1:3764156/1‑101 (MQ=255)
aCCTATGGCGAGCAGGTGGGGGCGACGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCAg                                                                                                  <  2:3284536/101‑1 (MQ=255)
  cTATGGCGAGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCAgac                                                                                                >  2:2811905/1‑99 (MQ=255)
  cTATGGCGAGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCAgac                                                                                                <  1:2834485/101‑3 (MQ=255)
  cTATGGCGAGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCAGAGGTCCGCAGATGAGCCCCGATGACCTGCAgac                                                                                                <  1:4726115/101‑3 (MQ=255)
   tATGGCGAGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCAgacg                                                                                               >  2:3474209/1‑98 (MQ=255)
     tGGCGAGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGc                                                                                            <  1:2557336/101‑1 (MQ=255)
        cGAGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCg                                                                                         >  2:1793087/1‑101 (MQ=255)
        cGAGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCg                                                                                         <  2:849467/101‑1 (MQ=255)
          aGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCggg                                                                                       >  2:199508/1‑101 (MQ=255)
          aGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCggg                                                                                       <  2:3350275/101‑1 (MQ=255)
             aGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGgcgc                                                                                    >  1:2160412/1‑101 (MQ=255)
             aGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGgcgc                                                                                    <  1:2187580/101‑1 (MQ=255)
             aGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGgcgc                                                                                    >  2:3157111/1‑101 (MQ=255)
              ggTGGGGGCGATGCATGTGAACCAGACCCTGCCCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCt                                                                                   <  1:4199247/101‑1 (MQ=255)
                tGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTgg                                                                                 >  1:3946285/1‑101 (MQ=255)
                 gggggCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGt                                                                                <  2:2458839/101‑1 (MQ=255)
                 gggggCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGt                                                                                >  1:3497899/1‑101 (MQ=255)
                 gggggCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGt                                                                                >  2:4093776/1‑101 (MQ=255)
                 gggggCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGt                                                                                >  1:329574/1‑101 (MQ=255)
                  ggggCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTc                                                                               <  1:1887079/101‑1 (MQ=255)
                  gatctgATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTc                                                                               <  2:1381008/96‑1 (MQ=255)
                   gggCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCg                                                                              <  1:2394768/101‑1 (MQ=255)
                      cGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATc                                                                           >  2:1871147/1‑101 (MQ=255)
                       gATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATcc                                                                          >  2:428979/1‑101 (MQ=255)
                       gATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATcc                                                                          >  1:3867142/1‑101 (MQ=255)
                       gATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATcc                                                                          >  2:4582731/1‑101 (MQ=255)
                        aTGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCcg                                                                         <  2:1218153/101‑1 (MQ=255)
                        aTGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCcg                                                                         >  1:2761653/1‑101 (MQ=255)
                          gCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCcgcg                                                                       <  1:1380442/101‑1 (MQ=255)
                                gAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCAGG.CGGCCCGGGCGCTGGTCGATCCGCGCGATcc                                                                 <  1:3126975/101‑1 (MQ=255)
                                gAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATcc                                                                 <  1:122540/101‑1 (MQ=255)
                                 aaCCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCg                                                                >  2:2115078/1‑101 (MQ=255)
                                  aCCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGc                                                               <  2:3736610/101‑1 (MQ=255)
                                   ccAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGcc                                                              <  1:729229/101‑1 (MQ=255)
                                    cAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGccc                                                             <  2:2245049/101‑1 (MQ=255)
                                     aGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCg                                                            <  1:1033853/101‑1 (MQ=255)
                                         ccTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCg                                                        <  2:1157773/101‑1 (MQ=255)
                                          cTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCgg                                                       >  1:664497/1‑101 (MQ=255)
                                           tGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGc                                                      <  1:3648112/101‑1 (MQ=255)
                                            gACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGGAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCa                                                     >  2:2907027/1‑101 (MQ=255)
                                            gACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCa                                                     <  1:4582984/101‑1 (MQ=255)
                                            gACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCa                                                     <  2:3097369/101‑1 (MQ=255)
                                            gACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCa                                                     <  1:3857772/101‑1 (MQ=255)
                                              ccGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGGCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGa                                                   >  1:1196695/1‑101 (MQ=255)
                                               cGGGCTGTCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGAc                                                  <  2:2136198/101‑1 (MQ=255)
                                               cGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGAc                                                  >  2:2736058/1‑101 (MQ=255)
                                                gggCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGAcc                                                 <  2:548980/101‑1 (MQ=255)
                                                gggCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGAGGACCTGCA.GACGGCCCGGGGGCTCGCCGATCCGCGCGCGCCGCCCGAGCGGCAGAcc                                                 >  1:4054763/1‑101 (MQ=255)
                                                 ggCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCg                                                <  1:794831/101‑1 (MQ=255)
                                                 ggCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCg                                                <  1:280730/101‑1 (MQ=255)
                                                 ggCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCg                                                <  2:3986844/101‑1 (MQ=255)
                                                   cTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCgag                                              >  1:1544269/1‑101 (MQ=255)
                                                   cTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCgag                                              >  1:2053208/1‑101 (MQ=255)
                                                   cTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCgag                                              >  2:1600419/1‑101 (MQ=255)
                                                    tGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCgaga                                             >  2:926353/1‑101 (MQ=255)
                                                    tGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCgaga                                             >  2:1054971/1‑101 (MQ=255)
                                                     ggCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCgagag                                            >  2:129295/1‑100 (MQ=255)
                                                     ggCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGaa                                            <  2:2668912/101‑1 (MQ=255)
                                                     ggCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGaa                                            <  2:706641/101‑1 (MQ=255)
                                                        cTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCt                                         >  1:3095056/1‑101 (MQ=255)
                                                        cTTTTATCCGCCCGAGGCCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGCGCGGCAGACCGAGAAGCt                                         >  1:1752090/1‑101 (MQ=255)
                                                         ttttATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCtt                                        >  2:323937/1‑101 (MQ=255)
                                                         ttttATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCtt                                        >  1:644829/1‑101 (MQ=255)
                                                         ttttATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCtt                                        >  2:46608/1‑101 (MQ=255)
                                                           ttATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCt                                      >  1:1590421/1‑101 (MQ=255)
                                                             aTCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGa                                    <  2:3946043/101‑1 (MQ=255)
                                                               ccGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGAcc                                  <  1:3543942/101‑1 (MQ=255)
                                                               ccGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGAcc                                  >  1:670317/1‑101 (MQ=255)
                                                                cGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCa                                 <  2:1544168/101‑1 (MQ=255)
                                                                 gCCCGAGGTCCGCGGATGAGCCCCGATGACCTACA.GAGGGCCCGGGGGCTGGTCGATCCGCGCGTTCCGCCCGAGCGGCAGACCGAGAAGCTTCTGAcatc                                <  2:2774440/101‑4 (MQ=255)
                                                                 ccccGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCAt                                <  2:119540/100‑1 (MQ=255)
                                                                  cccGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCAtc                               <  1:1029838/101‑1 (MQ=255)
                                                                  cccGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCAtc                               >  2:4321715/1‑101 (MQ=255)
                                                                    cGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCAtcgt                             >  1:843030/1‑101 (MQ=255)
                                                                    cGAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCAtcgt                             >  2:2534250/1‑101 (MQ=255)
                                                                     gAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCAtcgtc                            >  1:244301/1‑101 (MQ=255)
                                                                     gAGGTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCAtcgtc                            <  1:3420476/101‑1 (MQ=255)
                                                                        gTCCGCAGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGgc                         >  1:4118423/1‑101 (MQ=255)
                                                                         cccggatctGAGCCCCGAGCGCCTGCA.GACGGCCCGGTCGCTGGCCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGgcg                        <  2:1782700/93‑1 (MQ=255)
                                                                              aGATGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTg                   >  2:3271833/1‑101 (MQ=255)
                                                                                atcaGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGAt                 >  1:2712812/4‑101 (MQ=255)
                                                                                 tGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATc                >  1:133660/1‑101 (MQ=255)
                                                                                 tGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATc                <  1:2583407/101‑1 (MQ=255)
                                                                                 tGAGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATc                >  2:363892/1‑101 (MQ=255)
                                                                                   aGCCCCGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCg              <  2:4101018/101‑1 (MQ=255)
                                                                                     ccccGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGcc            <  1:1403813/101‑1 (MQ=255)
                                                                                     ccccGATGACCTGC.GGACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGcc            <  2:3859415/101‑1 (MQ=255)
                                                                                       ccGATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCAt          <  1:926430/101‑1 (MQ=255)
                                                                                       cagATGTCCTGCAAG.CCGCCCGAGCGCTGGTCGATCCGCGCGATCCGCCTGAGCGGCAGACCGAAAAGCTTCTGAACATCGTTGGCGCGCTGATCTGCCAt          <  2:1567851/99‑1 (MQ=255)
                                                                                         gATGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCATGc        >  2:229353/1‑101 (MQ=255)
                                                                                          aggACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCATGcc       <  2:1944781/99‑1 (MQ=255)
                                                                                          aTGACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCATGcc       >  2:4744164/1‑101 (MQ=255)
                                                                                            gACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCATGCCGa     <  1:332568/101‑1 (MQ=255)
                                                                                            gACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCATGCCGa     >  2:3823419/1‑101 (MQ=255)
                                                                                            gACCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCATGCCGa     <  2:664433/101‑1 (MQ=255)
                                                                                             aCCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCATGCCGAg    >  2:4532665/1‑101 (MQ=255)
                                                                                             aCCTGCA.GACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCATCTGACCATCGTCGGCGCGCTGATCCGCCATGCCGAg    <  1:3284495/101‑1 (MQ=255)
                                                                                             aCCTGCA.GACGGCCCGGGCGCTGGACGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCATGCCGAg    >  2:3569464/1‑101 (MQ=255)
                                                                                                    |                                                                                                
ACCTATGGCGAGCAGGTGGGGGCGATGCATGTGAACCAGACCCTGACCGGGCTGGCCTTTTATCCGCCCGAGGTCCGCAGATGAGCCCCGATGACCTGCAGGACGGCCCGGGCGCTGGTCGATCCGCGCGATCCGCCCGAGCGGCAGACCGAGAAGCTTCTGACCATCGTCGGCGCGCTGATCCGCCATGCCGAGGA  >  NC_014034/1796887‑1797083

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG