Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,960,668 Δ1 bp 96.5% coding (1111/1539 nt) RCAP_rcc01813 ← AMP‑dependent synthetase and ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,960,6680G→.96.5% 15.2 ‑2.0 ‑0.3202coding (1111/1539 nt)RCAP_rcc01813AMP‑dependent synthetase and ligase
Reads supporting (aligned to +/- strand):  new base (87/108):  ref base (1/6):  total (88/114)
Fisher's exact test strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.03e-01

Only 100 of 216 total aligned reads displayed.

CCTGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGG.CTCGGCGGCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCTCCGGGGGACAGGAC  >  NC_014034/1960569‑1960764
                                                                                                    |                                                                                                 
 cTGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGACCCAGTCCCCTTCCGCGGTGAACTTTGCCCGCGGCTCGGCCTcggcgc                                                                                                  >  2:1621764/1‑100 (MQ=255)
  tGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTcggcgcc                                                                                                 <  2:2294998/101‑3 (MQ=255)
  tGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTcggcgcc                                                                                                 >  1:528009/1‑99 (MQ=255)
   gATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGCGCcc                                                                                                <  1:906977/101‑1 (MQ=255)
    aTAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGCGCccc                                                                                               >  1:3481474/1‑101 (MQ=255)
    aTAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGCGCccc                                                                                               <  1:1847187/101‑1 (MQ=255)
       ggCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAAt                                                                                           >  2:1925832/1‑101 (MQ=255)
        gCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATa                                                                                          >  2:212744/1‑101 (MQ=255)
         cGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAg                                                                                         <  1:3270715/101‑1 (MQ=255)
         cGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAg                                                                                         <  1:1479446/101‑1 (MQ=255)
         cGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAg                                                                                         <  2:2699983/101‑1 (MQ=255)
          gATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGc                                                                                        >  2:2487077/1‑101 (MQ=255)
          gATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGc                                                                                        >  1:4489591/1‑101 (MQ=255)
           aTGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGcc                                                                                       >  2:2585274/1‑101 (MQ=255)
           aTGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGcc                                                                                       >  1:4190795/1‑101 (MQ=255)
           aTGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCCCGGCCCCGGCGCccccctaacc                                                                                        >  1:858610/1‑95 (MQ=255)
               ccccGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAgg                                                                                   <  1:2534217/101‑1 (MQ=255)
                 ccGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCACGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGaa                                                                                 <  1:4016353/101‑1 (MQ=255)
                 ccGGCCTCGGTCATCCGCACGATGTCACCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGaa                                                                                 <  1:4219284/101‑1 (MQ=255)
                  cGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGaaa                                                                                <  2:2023718/101‑1 (MQ=255)
                   ggCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGaaaa                                                                               >  1:3977342/1‑101 (MQ=255)
                   ggCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGaaaa                                                                               <  2:4061044/101‑1 (MQ=255)
                   ggCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGaaaa                                                                               <  1:713168/101‑1 (MQ=255)
                     ccTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGc                                                                             <  1:3432104/101‑1 (MQ=255)
                         ggTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCg                                                                         <  1:1597816/101‑1 (MQ=255)
                          gTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCgg                                                                        <  1:3681632/101‑1 (MQ=255)
                          gTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCgg                                                                        <  1:1465260/101‑1 (MQ=255)
                           tCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGa                                                                       >  1:3038595/1‑101 (MQ=255)
                            cATCCGCACGATGTCGCCGGTGCGGACCCAGTCCCCGTCCGCAGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGAt                                                                      <  2:2670032/101‑1 (MQ=255)
                            cATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGAt                                                                      >  2:882244/1‑101 (MQ=255)
                            cATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAACAGCCGAGGAAAAGCCCCGGAt                                                                      <  1:2699905/101‑1 (MQ=255)
                             aTCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGAtt                                                                     <  1:4560611/101‑2 (MQ=255)
                             aTCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATc                                                                     <  1:1197276/101‑1 (MQ=255)
                             aTCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATc                                                                     <  1:346715/101‑1 (MQ=255)
                             aTCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATc                                                                     <  2:1047725/101‑1 (MQ=255)
                                cGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCt                                                                  <  1:56453/101‑1 (MQ=255)
                                 gCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTg                                                                 >  1:666565/1‑101 (MQ=255)
                                 gCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTg                                                                 >  1:1685254/1‑101 (MQ=255)
                                  cACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGc                                                                <  1:1951192/101‑1 (MQ=255)
                                   aCGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCa                                                               <  1:2351605/101‑1 (MQ=255)
                                   aCGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCa                                                               <  2:4697690/101‑1 (MQ=255)
                                   aCGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCa                                                               <  1:2137379/101‑1 (MQ=255)
                                   aCGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCa                                                               >  1:3936317/1‑101 (MQ=255)
                                   aCGATGTCGCCGGTGCGGAACCAGTCCCCGGCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCa                                                               <  1:3312595/101‑1 (MQ=255)
                                     gATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCCAt                                                             >  2:3132113/1‑101 (MQ=255)
                                     gATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAAt                                                             <  2:3350909/101‑1 (MQ=255)
                                       tGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGc                                                           >  1:3184119/1‑101 (MQ=255)
                                           gCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCg                                                       <  2:1494904/101‑1 (MQ=255)
                                           gCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCg                                                       <  2:35360/101‑1 (MQ=255)
                                           gCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCg                                                       <  1:3623499/101‑1 (MQ=255)
                                            ccGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGa                                                      <  1:2875501/101‑1 (MQ=255)
                                             cGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGAc                                                     <  2:2217628/101‑1 (MQ=255)
                                              gttGCGCAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACa                                                    <  2:715845/99‑1 (MQ=255)
                                              ggTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACa                                                    <  1:2590365/101‑1 (MQ=255)
                                               gTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAg                                                   <  2:3400924/101‑1 (MQ=255)
                                                  cGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTc                                                >  2:2864933/1‑101 (MQ=255)
                                                   ggAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCg                                               >  2:3592811/1‑101 (MQ=255)
                                                   ggAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCg                                               >  2:1363094/1‑101 (MQ=255)
                                                   ggAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCg                                               >  1:4739772/1‑101 (MQ=255)
                                                    gAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGc                                              >  2:2988003/1‑101 (MQ=255)
                                                     aaCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGcc                                             >  2:4678732/1‑101 (MQ=255)
                                                     aaCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGcc                                             <  1:620582/101‑1 (MQ=255)
                                                      aCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGccc                                            <  1:2272881/101‑1 (MQ=255)
                                                       ccAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCCCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCg                                           <  1:3866405/101‑1 (MQ=255)
                                                        cAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCgg                                          >  1:3404230/1‑101 (MQ=255)
                                                         aGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGt                                         <  1:140499/101‑1 (MQ=255)
                                                            ccccGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCg                                      >  2:4516366/1‑101 (MQ=255)
                                                            ccccGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCg                                      <  1:3019792/101‑1 (MQ=255)
                                                            ccccGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCg                                      >  1:745552/1‑101 (MQ=255)
                                                            ccccGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCg                                      >  2:125311/1‑101 (MQ=255)
                                                              ccGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGcc                                    >  2:3872254/1‑101 (MQ=255)
                                                                   cgcgGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCggg                               >  1:1067155/1‑101 (MQ=255)
                                                                    gcgGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCgggg                              <  1:2687101/101‑1 (MQ=255)
                                                                       gTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCAc                           >  2:2530702/1‑101 (MQ=255)
                                                                         gAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCg                         <  2:4090480/101‑1 (MQ=255)
                                                                         gAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCg                         <  2:2878065/101‑1 (MQ=255)
                                                                            cTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGAt                      <  1:2009081/101‑1 (MQ=255)
                                                                            cTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGAt                      >  1:2079113/1‑101 (MQ=255)
                                                                            cTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGAt                      <  1:2093615/101‑1 (MQ=255)
                                                                            cTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGAt                      <  1:2491802/101‑1 (MQ=255)
                                                                             tttGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATg                     <  1:3746225/101‑1 (MQ=255)
                                                                              ttGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATgg                    >  2:1449288/1‑101 (MQ=255)
                                                                              ttGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATgg                    >  1:310458/1‑101 (MQ=255)
                                                                                  ccGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATCG.ccct               >  2:3945799/1‑99 (MQ=255)
                                                                                   cgcgCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.ccctc              >  1:2123704/1‑98 (MQ=255)
                                                                                   cgcgCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.ccctc              <  2:4190549/101‑4 (MQ=255)
                                                                                     cgcCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.ccctcgg            <  1:1323491/101‑6 (MQ=255)
                                                                                      gcCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCCgg            >  2:4039028/1‑101 (MQ=255)
                                                                                       ccTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCgggg          <  2:3038407/101‑1 (MQ=255)
                                                                                       ccTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCgggg          >  2:2208225/1‑101 (MQ=255)
                                                                                        ctcggcctcgGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCggggg         >  1:3548029/1‑101 (MQ=255)
                                                                                         tcggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCGGGGGa        <  2:4442869/96‑1 (MQ=255)
                                                                                          cggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCGGGGGAc       >  1:2815162/5‑101 (MQ=255)
                                                                                          cggcctcggc.GCCCCAATAGCCGAGGAAAAGACCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCGGGGGAc       >  1:4234138/5‑101 (MQ=255)
                                                                                           ggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCGGGGGACa      >  1:989719/4‑101 (MQ=255)
                                                                                            gcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCGGGGGACAg     <  2:2398355/99‑1 (MQ=255)
                                                                                            gcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCGGGGGACAg     <  1:4397700/99‑1 (MQ=255)
                                                                                             cctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCGGGGGACAgg    >  1:1557930/2‑101 (MQ=255)
                                                                                              ctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCGGGGGACAGGa   >  2:1989400/1‑101 (MQ=255)
                                                                                               tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCCTCgggggccggacc  >  1:2051833/1‑94 (MQ=255)
                                                                                                    |                                                                                                 
CCTGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGG.CTCGGCGGCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGG.CCTCCGGGGGACAGGAC  >  NC_014034/1960569‑1960764

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG