Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,974,355 Δ1 bp 100% intergenic (‑48/+7) rpe1 ← / ← cbbM ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,974,3550C→.100.0% 946.0 218intergenic (‑48/+7)rpe1/cbbMribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand):  new base (105/111):  ref base (0/1):  total (106/112)

Only 100 of 243 total aligned reads displayed.

TCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..G.GGC.GGGGTGT.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTCGCGATG  >  NC_014034/1974258‑1974451
                                                                                                       |                                                                                                
tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGCGTCC.CCCCGCCCGGATAGCAc                                                                                                  >  2:1664686/1‑101 (MQ=255)
 cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGGGTCC.CCCCGCCCGGATAGCAcg                                                                                                 >  1:3377180/1‑100 (MQ=255)
     aGAAAGGATCGAGGGGGCGATGTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGGGTCC.CCCCGCCCGGATAGCA.CGGCGt                                                                                            >  1:3500160/1‑101 (MQ=255)
      gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCGTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGtt                                                                                           <  2:749978/101‑1 (MQ=255)
      gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCAcagatcg                                                                                            >  1:1402521/1‑95 (MQ=255)
       gaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTc                                                                                          <  2:1361085/100‑1 (MQ=255)
       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTc                                                                                          <  1:454265/101‑1 (MQ=255)
       aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTc                                                                                          <  1:1939146/101‑1 (MQ=255)
        aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGT.CGCCCCGCCCGGATAGCA.CGGCGTTCa                                                                                         >  2:4706456/1‑101 (MQ=255)
        aaGGATCGAGGGAGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                                                         >  1:2565757/1‑101 (MQ=255)
            aTCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGttc                                                                                     <  1:2918092/101‑2 (MQ=255)
               gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGACGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                                                  >  1:1041786/1‑101 (MQ=255)
                   gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                                              <  2:12007/101‑1 (MQ=255)
                   gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                                              <  1:343784/101‑1 (MQ=255)
                    ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                                             <  2:464022/101‑1 (MQ=255)
                     gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                                            <  1:1226201/101‑1 (MQ=255)
                     gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                                            <  2:4180979/101‑1 (MQ=255)
                     gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                                            >  2:2134043/1‑101 (MQ=255)
                     gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                                            <  2:3947120/101‑1 (MQ=255)
                     gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                                            <  2:1116362/101‑1 (MQ=255)
                      cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCatag                                                                           <  1:2712582/101‑1 (MQ=255)
                      cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                                           >  2:352117/1‑101 (MQ=255)
                        aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                                                         <  1:2590427/101‑1 (MQ=255)
                        aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                                                         >  1:1544956/1‑101 (MQ=255)
                        aTCTTGATGGAACGGTCGAAGGCCATGTCGGTCTCCTGAGGGGTTGGG.GGG.GGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGCTCAGTTCCCGCCCAGGGCg                                                                         >  1:434825/1‑101 (MQ=255)
                          cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                                       <  2:498997/101‑1 (MQ=255)
                            tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                                                     >  2:4487067/1‑101 (MQ=255)
                            tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                                                     >  1:4431131/1‑101 (MQ=255)
                            tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                                                     >  2:1253770/1‑101 (MQ=255)
                              aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACgcgc                                                                   >  2:1154860/1‑101 (MQ=255)
                                ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                                                 <  2:1795367/101‑1 (MQ=255)
                                 gAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAg                                                                >  1:2794779/1‑101 (MQ=255)
                                 gAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAg                                                                <  1:2225294/101‑1 (MQ=255)
                                  aaCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGc                                                               >  2:2844603/1‑101 (MQ=255)
                                  aaCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGc                                                               >  1:1736196/1‑101 (MQ=255)
                                    cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                                                             >  2:2880545/1‑101 (MQ=255)
                                     ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                                                            <  1:1492779/101‑1 (MQ=255)
                                     ggTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCg                                                            >  2:3139942/1‑101 (MQ=255)
                                      gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCTACGCGCCAGCCCgg                                                           >  1:1912496/1‑101 (MQ=255)
                                      gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                                                           >  2:1438702/1‑101 (MQ=255)
                                        cGAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGGGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                                         >  2:1696169/1‑101 (MQ=255)
                                         gAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                                        >  2:2246729/1‑101 (MQ=255)
                                         gAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                                        <  1:1346452/101‑1 (MQ=255)
                                         gAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                                        >  2:4061073/1‑101 (MQ=255)
                                         gAAGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                                                        <  2:4586840/101‑1 (MQ=255)
                                          aaGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                                       >  2:1419532/1‑101 (MQ=255)
                                          aaGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                                       <  1:1616841/101‑1 (MQ=255)
                                          aaGGTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                                                       <  2:3813082/101‑1 (MQ=255)
                                             gTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGc                                                    <  1:3522332/101‑1 (MQ=255)
                                             gTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGc                                                    >  1:2421533/1‑101 (MQ=255)
                                             gTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGc                                                    <  1:2478146/101‑1 (MQ=255)
                                             gTCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGc                                                    >  2:2750204/1‑101 (MQ=255)
                                              tCATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCt                                                   >  2:3869656/1‑101 (MQ=255)
                                               cATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                                  <  1:561190/101‑1 (MQ=255)
                                               cATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                                  >  1:3272531/1‑101 (MQ=255)
                                               cATGTCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                                  >  2:614408/1‑101 (MQ=255)
                                                   tCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg                                              <  1:3736997/101‑1 (MQ=255)
                                                   tCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg                                              >  2:1596399/1‑101 (MQ=255)
                                                   tCGGTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg                                              >  1:4492442/1‑101 (MQ=255)
                                                      gTCTCCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCa                                           >  1:3927286/1‑101 (MQ=255)
                                                        ctcCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                                         <  2:4231725/101‑1 (MQ=255)
                                                        ctcCTGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                                         <  1:2011974/101‑1 (MQ=255)
                                                            tGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                                     >  1:3100479/1‑101 (MQ=255)
                                                            tGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                                     <  1:461383/101‑1 (MQ=255)
                                                            tGAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTCCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                                     <  1:2198446/101‑1 (MQ=255)
                                                            tGAGGGGTT..GAGGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                                     >  1:4104589/1‑101 (MQ=255)
                                                             gAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                                    <  2:342137/101‑1 (MQ=255)
                                                             gAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                                    <  1:1685876/101‑1 (MQ=255)
                                                             gAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                                    <  1:579309/101‑1 (MQ=255)
                                                             gAGGGGTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGACCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                                    >  1:2533769/1‑101 (MQ=255)
                                                              gggggTTG.G.GGCGGGGGTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGGCGAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCgg                                   <  2:3228921/100‑1 (MQ=255)
                                                                      tggggcgggggTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt                             >  1:1171885/8‑101 (MQ=255)
                                                                      tggggcgggggTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt                             >  1:917215/8‑101 (MQ=255)
                                                                      tggggcgggggTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt                             >  2:1890188/8‑101 (MQ=255)
                                                                      tggggcgggggTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt                             >  2:1549717/8‑101 (MQ=255)
                                                                       ggggcgggggTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt                            <  2:2582939/95‑1 (MQ=255)
                                                                        gggcgggggTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCttt                           <  2:1368143/96‑1 (MQ=255)
                                                                         ggcgggggTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc                          <  2:2185002/97‑1 (MQ=255)
                                                                             ggggTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAAc                      <  1:689202/101‑1 (MQ=255)
                                                                              gggTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACg                     <  2:2421398/101‑1 (MQ=255)
                                                                              gggTGTCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACg                     >  1:2314616/1‑101 (MQ=255)
                                                                                 tggcc.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGccc                  <  1:3123163/97‑1 (MQ=255)
                                                                                  gtcc.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCg                 >  1:4553584/4‑101 (MQ=255)
                                                                                   tcc.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCgg                >  1:964251/3‑101 (MQ=255)
                                                                                    cc.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCggg               >  2:1314394/2‑101 (MQ=255)
                                                                                     c.ccccGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGc              <  2:3088821/101‑1 (MQ=255)
                                                                                        cccgcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCa            >  1:3981393/1‑101 (MQ=255)
                                                                                        cccgcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCa            <  1:3817910/101‑1 (MQ=255)
                                                                                         ccgcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAg           <  2:2032491/101‑1 (MQ=255)
                                                                                          cgcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGt          <  2:631394/101‑1 (MQ=255)
                                                                                          cgcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGt          <  2:2276475/101‑1 (MQ=255)
                                                                                           gcccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGtt         <  1:2134164/101‑1 (MQ=255)
                                                                                            cccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTc        <  2:4312241/101‑1 (MQ=255)
                                                                                            cccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTc        <  1:3299698/101‑1 (MQ=255)
                                                                                            cccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTc        <  1:2750368/101‑1 (MQ=255)
                                                                                            cccgGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTCCGGGAAGCTTTCGAACGCCCGGGCCAGTTc        <  1:4226379/101‑1 (MQ=255)
                                                                                               ggATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTcgcg     >  2:759933/1‑101 (MQ=255)
                                                                                               ggATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTcgcg     <  2:4727163/101‑1 (MQ=255)
                                                                                               ggATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTcgcg     <  2:3327200/101‑1 (MQ=255)
                                                                                                gATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCACGGGCCAGTTCgcga    <  1:4711107/101‑1 (MQ=255)
                                                                                                       |                                                                                                
TCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..G.GGC.GGGGTGT.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGCCCGGGCCAGTTCGCGATG  >  NC_014034/1974258‑1974451

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG