Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,976,895 Δ1 bp 9.6% intergenic (‑74/+6) fba ← / ← gap1 fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,976,8950C→. 9.6% 58.1 ‑1.1 ‑1.2208intergenic (‑74/+6)fba/gap1fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (10/10):  ref base (115/73):  total (125/83)
Fisher's exact test strand distribution p-value = 3.47e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.56e-02

Only 100 of 212 total aligned reads displayed.

GCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCGGCGTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976800‑1976990
                                                                                                    |                                                                                               
gCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTtc                                                                                            >  2:1082203/1‑101 (MQ=255)
gCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTtc                                                                                            >  1:2272500/1‑101 (MQ=255)
  cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTtctc                                                                                          >  2:2671373/1‑101 (MQ=255)
   gAAGGGTGATCAGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCa                                                                                         >  1:2064924/1‑101 (MQ=255)
    aaggtttttCAGGGCTATTTTCTCGGTTCCTT.GGTTTCGCGGCCTTTTTCTTGGCCA.AAATCCGCCGGGGGCCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAg                                                                                        <  2:3870290/93‑1 (MQ=16)
       ggTGATCAGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATc                                                                                     >  1:2928737/1‑101 (MQ=255)
         tGATCAGTGCCTTTTTCTCAGTTCCTG.GAGTTCGCGGCCTTTTTCTGGGCCG.AAATCCGCCGGGGGCCG.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAg                                                                                   <  2:2968842/101‑1 (MQ=25)
         tGATCAGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAg                                                                                   >  2:1810140/1‑101 (MQ=255)
          gATCAGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGc                                                                                  >  2:2230140/1‑101 (MQ=255)
              aGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGc                                                                              >  2:449928/1‑101 (MQ=255)
              aGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGc                                                                              >  1:1782974/1‑101 (MQ=255)
                tgcttttttCTCAGTTCCTT.GAGTTGGCGGCTTTTTTCTGGGCAG.AAATACGCCGGGGGCCG.GGGGG.CACCC.CCCCCGGCTTTTCTCAGATCAGCTTGccc                                                                            <  1:3153269/96‑1 (MQ=255)
                tGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGccc                                                                            >  2:4567527/1‑101 (MQ=255)
                tGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGccc                                                                            >  1:4351473/1‑101 (MQ=255)
                  cttttttCTCTTTTCTTT.GTTTTCGCGCCTTTTTTCTGGGCAG.AAATCCGCGGGGGGCCG.GGGGG.CCGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCAt                                                                          <  1:433315/98‑1 (MQ=255)
                  cctttttCTCATTTCTTT.GGTTCCGGGGCTTTTTTCTGGCCCA.AAATCCGCGGGGGGCCG.GGGGG.CGGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCAt                                                                          <  1:4417397/98‑1 (MQ=255)
                  ccATTTTCTCAGTTGCTT.GACTTCGGCGCCTTTTTCTTGGCCA.AAAAAGCCCGGGGGTCG.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCAt                                                                          <  2:3065145/101‑1 (MQ=21)
                  ccATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGCG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCAt                                                                          >  1:3310747/1‑101 (MQ=37)
                   cATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATc                                                                         >  1:3946669/1‑101 (MQ=255)
                    tttttCTC.GTTCTTTGGAGTTCGCGCCTTTTTTCTTGGCAA.AAATGCGCGGGGGGCGG.GGGGG.CGCCC.CCCCGGGCTTTTCTCAGATCAGCTTGCCCATCg                                                                        <  1:1263544/100‑1 (MQ=255)
                    aTTTTCTCAGTTCCTT.GAGTTCGGTGCCTTTTTCTGGGCCA.AAAAAGGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCg                                                                        <  2:3896743/101‑1 (MQ=25)
                     ttttCCGAGTTCCTT.GAGTTCGCGCCCTTTTTCTTGGCCA.AAATACCCCGGGGTGCG.GGGGG.CGGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGc                                                                       <  2:3505881/101‑1 (MQ=255)
                      tttCTCTGTT.CTT.GGGTTCCGCGCCTTTTTTCGGGCCGAAAACCCGCGGGGGGCCG.GGGGG.CGGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGcc                                                                      <  1:280234/101‑1 (MQ=255)
                      tttCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGcc                                                                      >  2:3466722/1‑101 (MQ=255)
                      tttCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGcc                                                                      >  2:1290372/1‑101 (MQ=255)
                        tctcAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    >  2:843813/1‑101 (MQ=255)
                         ctcAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcg                                                                   >  2:113302/1‑101 (MQ=255)
                         ctcAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcg                                                                   >  1:1361999/1‑101 (MQ=255)
                           cAGTTCCTT.GAGTGCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCG.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggc                                                                 <  2:4626740/101‑1 (MQ=25)
                              ttCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAccgcggg                                                              >  1:3575105/1‑100 (MQ=25)
                              ttCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              >  1:3803657/1‑101 (MQ=255)
                              ttCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              >  2:3203563/1‑101 (MQ=255)
                              ttCCTT.GAGTTCGCGCCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              <  2:682221/101‑1 (MQ=25)
                               tCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  1:1617015/1‑101 (MQ=255)
                                 cTT.GAGTTCGCGGCTTTTTTCTTGCCCG.AAATCCGCCGGGGGTCC.GGGGG.CGGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATc                                                           <  1:2114632/101‑1 (MQ=25)
                                   t.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         >  1:560354/1‑101 (MQ=255)
                                     gAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCCCGGCGGTATCGGc                                                        >  2:2563320/1‑101 (MQ=37)
                                     gAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGc                                                        >  2:1935992/1‑101 (MQ=255)
                                       gTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCCTCGCCACGGCGGCATCGGCCa                                                      >  2:2210067/1‑101 (MQ=25)
                                        ttCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCAt                                                     >  1:4514920/1‑101 (MQ=255)
                                         tCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATg                                                    >  1:2034813/1‑101 (MQ=255)
                                         ggcgtgcttttttTCTGGCCA.AAACCCGGGGGGGGTCG.GGGGGCCCCCC.CCCCGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCAt                                                     <  1:2486702/93‑1 (MQ=9)
                                             ggCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGt                                                >  1:1851972/1‑101 (MQ=255)
                                             ggCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGGATCGGCCATGCGGt                                                >  2:2281954/1‑101 (MQ=37)
                                               ccTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              >  1:921455/1‑101 (MQ=255)
                                                cTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             >  2:3023449/1‑101 (MQ=255)
                                                  ttttCTTGGCCG.AAATCCGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           <  1:3091057/101‑1 (MQ=37)
                                                     tCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAg                                        >  1:4210491/1‑101 (MQ=255)
                                                     tCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAg                                        >  2:291294/1‑101 (MQ=255)
                                                      cTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       >  1:2547727/1‑101 (MQ=255)
                                                        tGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGGGAAGccc                                     >  2:3593236/1‑101 (MQ=25)
                                                         ggCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    >  2:3741453/1‑101 (MQ=255)
                                                         ggCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    >  1:1442175/1‑101 (MQ=255)
                                                         gcccG.AAATCCGCGGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    <  1:1975923/99‑1 (MQ=21)
                                                             g.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                >  2:4393155/1‑101 (MQ=255)
                                                             g.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                >  2:666354/1‑101 (MQ=255)
                                                              cgaaccccgcGGGGGCCCGGGGGG.CGCCC.CCCCGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 <  1:1111213/92‑1 (MQ=17)
                                                               aaaTGCGCCGGGGGTCG.GGGGC.CAGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               <  2:3793343/101‑1 (MQ=18)
                                                               aaaTACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               >  1:4745719/1‑101 (MQ=255)
                                                               aaaTACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               >  2:3357528/1‑101 (MQ=255)
                                                                aaTACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGTCCcattcat                              >  2:516714/1‑101 (MQ=25)
                                                                aaTACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              >  1:4436584/1‑101 (MQ=255)
                                                                 aTACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  2:1998524/1‑101 (MQ=255)
                                                                 aTACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             <  2:4351226/101‑1 (MQ=255)
                                                                 aTACGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  1:2358684/1‑101 (MQ=255)
                                                                   aCGCGGGGGGTCG.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGt                           <  2:3946427/101‑1 (MQ=18)
                                                                   aCGCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGt                           >  2:1760030/1‑101 (MQ=255)
                                                                   aCGCCGGGGGTCC.GGGGG.CACCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGt                           <  1:1525738/101‑1 (MQ=18)
                                                                     gCCGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         <  2:1851860/101‑1 (MQ=255)
                                                                       cGGGGGTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATC.GCCATGCGGTTCGAGGAGNCCCCTTCATTGTGAttc                      >  2:1711675/1‑99 (MQ=18)
                                                                        gggggTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      >  2:748114/1‑101 (MQ=255)
                                                                        gggggCCG.GGGGG.CGCCC.CCCCGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAc                      <  2:4153106/101‑1 (MQ=18)
                                                                         ggggTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     <  1:2230264/101‑1 (MQ=255)
                                                                          gggTCG.GGGGG.CAGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    <  2:4514684/101‑1 (MQ=18)
                                                                          gggTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    >  2:567456/1‑101 (MQ=255)
                                                                          gggCCC.GGGGG.CAGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    <  2:2523842/101‑1 (MQ=18)
                                                                           ggTCC.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCAc                   <  2:2876768/101‑1 (MQ=255)
                                                                            gTCC.GGGGG.CCGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  <  1:3041071/101‑1 (MQ=18)
                                                                             gccgggggg.CCCCC.CCCCGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCACTGTCATACCaag                  <  1:2578241/97‑3 (MQ=17)
                                                                              cc.GGGGG.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc                <  2:1474924/101‑1 (MQ=255)
                                                                               cgggggg.CAGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc                <  1:4544420/99‑1 (MQ=18)
                                                                               cgggggc.CAGCC.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc                <  1:1998641/99‑1 (MQ=18)
                                                                               c.GGGGG.CAGCGCCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa               >  2:3440913/1‑101 (MQ=255)
                                                                               c.GGGGG.CAGCGCCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa               >  1:2780223/1‑101 (MQ=255)
                                                                                 cggagcaaGCC.CCCCGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa               <  1:1490095/94‑1 (MQ=18)
                                                                                   ggg.CAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGa            <  2:2178268/101‑1 (MQ=255)
                                                                                      ccgccccccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATg          <  1:3203764/94‑1 (MQ=18)
                                                                                       cAGCG.CCCCGGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  2:480051/101‑1 (MQ=255)
                                                                                       cAGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  1:2671527/101‑1 (MQ=255)
                                                                                        cgcccccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        <  2:1204620/96‑1 (MQ=255)
                                                                                        aGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        >  2:1338120/1‑101 (MQ=255)
                                                                                        aGCG.CCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        <  1:612324/101‑1 (MQ=255)
                                                                                         ccccccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCgg       <  1:3088208/97‑1 (MQ=255)
                                                                                           ccccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     <  2:600955/99‑1 (MQ=255)
                                                                                            cccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    <  2:1552420/100‑1 (MQ=255)
                                                                                             ccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa   >  1:4267779/1‑101 (MQ=255)
                                                                                             ccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa   <  2:3083851/101‑1 (MQ=255)
                                                                                              cccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAt  <  2:42435/101‑1 (MQ=255)
                                                                                              cccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAt  <  2:1044768/101‑1 (MQ=255)
                                                                                              cccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAt  >  1:2070193/1‑101 (MQ=255)
                                                                                                    |                                                                                               
GCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTT.GAGTTCGCGGCCTTTTTCTTGGCCG.AAATACGCCGGGGGTCC.GGGGG.CAGCG.CCCCGGCGTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976800‑1976990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG