Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,027,931 Δ1 bp 100% intergenic (‑337/‑16) RCAP_rcc01873 ← / → RCAP_rcc01874 peptidoglycan‑binding domain 1 protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,027,9310T→.100.0% 918.7 207intergenic (‑337/‑16)RCAP_rcc01873/RCAP_rcc01874peptidoglycan‑binding domain 1 protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (112/94):  ref base (0/0):  total (113/94)

Only 100 of 221 total aligned reads displayed.

TAAGTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCTTTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTATTC  >  NC_014034/2027831‑2028026
                                                                                                    |                                                                                               
 aaGTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCtt                                                                                                >  1:501679/1‑101 (MQ=255)
 aaGTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCtt                                                                                                <  2:501628/101‑1 (MQ=255)
 aaGTCCGGAAAAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCtt                                                                                                <  1:2382103/101‑1 (MQ=255)
  aGTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCttt                                                                                               <  1:2524418/101‑1 (MQ=255)
   gTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCtttg                                                                                              >  1:596487/1‑100 (MQ=255)
       ggATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTtgctgc                                                                                         >  2:2684774/1‑101 (MQ=255)
       ggATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTtgctgc                                                                                         >  2:3907237/1‑101 (MQ=255)
        gATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGcc                                                                                        <  1:1269849/101‑1 (MQ=255)
        gATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGcc                                                                                        >  2:464021/1‑101 (MQ=255)
           cAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACg                                                                                     >  1:2584583/1‑101 (MQ=255)
                 gCAAACTCAGACGGCACAGACCGAGGGGCAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTtgtg                                                                               >  1:3143481/1‑101 (MQ=255)
                 gCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTtgtg                                                                               >  1:3901408/1‑101 (MQ=255)
                 gCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTtgtg                                                                               <  2:3060266/101‑1 (MQ=255)
                   aaaCTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGt                                                                             >  2:1085218/1‑101 (MQ=255)
                      cTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTagc                                                                          <  2:886097/101‑1 (MQ=255)
                      cTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTagc                                                                          >  2:4325056/1‑101 (MQ=255)
                       tCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTagca                                                                         <  2:2328809/101‑1 (MQ=255)
                        cAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTagcag                                                                        <  2:1797467/101‑1 (MQ=255)
                         aGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTagcagc                                                                       >  1:1182051/1‑101 (MQ=255)
                           aCGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGccc                                                                     <  1:1452633/101‑1 (MQ=255)
                            cGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGcccc                                                                    >  1:1686111/1‑101 (MQ=255)
                             ggCACAGACCGAGGGACAGGCGAACCGTTATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGCAGCAGCCCCg                                                                   <  2:1016703/101‑1 (MQ=255)
                              gCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGa                                                                  >  1:1762953/1‑101 (MQ=255)
                                acaGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGaaa                                                                >  1:1667724/1‑101 (MQ=255)
                                acaGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGaaa                                                                <  1:2723491/101‑1 (MQ=255)
                                 caGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAg                                                               <  1:464065/101‑1 (MQ=255)
                                 caGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAg                                                               >  2:4044094/1‑101 (MQ=255)
                                  aGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGc                                                              >  2:3213627/1‑101 (MQ=255)
                                   gACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGcc                                                             <  1:737293/101‑1 (MQ=255)
                                    aCCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCt                                                            >  2:2973587/1‑101 (MQ=255)
                                     ccGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTc                                                           <  2:4612100/101‑1 (MQ=255)
                                      cGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTcc                                                          <  2:1310609/101‑1 (MQ=255)
                                      cGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTcc                                                          >  1:3659852/1‑101 (MQ=255)
                                      cGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTcc                                                          <  1:3717840/101‑1 (MQ=255)
                                      cGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTcc                                                          <  1:400816/101‑1 (MQ=255)
                                       gAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCa                                                         <  1:636850/101‑1 (MQ=255)
                                       gAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGC.GCCCTTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTccc                                                         >  2:4354177/1‑100 (MQ=255)
                                        aGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCaa                                                        >  2:2453588/1‑101 (MQ=255)
                                        aGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCaa                                                        <  1:2048339/101‑1 (MQ=255)
                                         gggACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAAc                                                       <  1:938674/101‑1 (MQ=255)
                                         gggACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAAc                                                       >  2:3723629/1‑101 (MQ=255)
                                         gggACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAAc                                                       >  1:1949977/1‑101 (MQ=255)
                                          ggACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACg                                                      <  1:1035689/101‑1 (MQ=255)
                                          ggACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACg                                                      >  2:3620836/1‑101 (MQ=255)
                                           gACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGc                                                     >  1:4691837/1‑101 (MQ=255)
                                               ggCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTc                                                 >  1:4243692/1‑101 (MQ=255)
                                                gCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCa                                                <  2:543139/101‑1 (MQ=255)
                                                 cGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGCCAGCCTCCAACGCCTTCAg                                               >  2:3803802/1‑101 (MQ=255)
                                                  gAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGc                                              >  1:4547795/1‑101 (MQ=255)
                                                  gAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGGAGCAGCCCCGAAAGCCTCCAACGCCCTCAGc                                              >  1:2795259/1‑101 (MQ=255)
                                                      cGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTcc                                          >  2:4160212/1‑101 (MQ=255)
                                                       gATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCt                                         >  1:3392462/1‑101 (MQ=255)
                                                       gATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCt                                         >  2:2543239/1‑101 (MQ=255)
                                                        atatGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTg                                        >  1:581829/1‑101 (MQ=255)
                                                        atatGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTg                                        >  1:2652052/1‑101 (MQ=255)
                                                         tatGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTgc                                       >  2:4743433/1‑101 (MQ=255)
                                                           tGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTgcgc                                     >  1:2655982/1‑101 (MQ=255)
                                                             ggCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCgcgg                                   <  2:1036220/101‑1 (MQ=255)
                                                              gCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCgcggc                                  >  2:1592688/1‑101 (MQ=255)
                                                               cACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCgcggcg                                 >  1:820820/1‑101 (MQ=255)
                                                               cACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCgcggcg                                 >  2:4087552/1‑101 (MQ=255)
                                                                 cGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGgcgcg                               >  2:4684575/1‑100 (MQ=255)
                                                                  gATCACGCCTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTg                              <  2:596423/101‑1 (MQ=255)
                                                                  gATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTg                              >  2:3544503/1‑101 (MQ=255)
                                                                  gATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTg                              <  1:3907479/101‑1 (MQ=255)
                                                                  gATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTg                              >  1:3269666/1‑101 (MQ=255)
                                                                   aTCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTgg                             <  2:1695551/101‑1 (MQ=255)
                                                                   aTCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTgg                             >  2:1895955/1‑101 (MQ=255)
                                                                     cACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCg                           <  1:2684928/101‑1 (MQ=255)
                                                                      aCGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGa                          >  2:4200585/1‑101 (MQ=255)
                                                                      aCGACTTCTCGTCGCGGGCTTATTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGa                          >  1:3497353/1‑101 (MQ=255)
                                                                        gACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGata                        <  2:765049/101‑1 (MQ=255)
                                                                        gACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGata                        <  2:1642916/101‑1 (MQ=255)
                                                                          cTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATc                      <  1:2973344/101‑1 (MQ=255)
                                                                          cTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATc                      <  2:2072475/101‑1 (MQ=255)
                                                                          cTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATc                      <  2:2779023/101‑1 (MQ=255)
                                                                          cTTCTCGTCGCGGCCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATc                      <  2:1409231/101‑1 (MQ=255)
                                                                               cgtcgCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCg                 >  2:4141976/1‑101 (MQ=255)
                                                                                gtcgCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCgg                >  1:3081542/1‑101 (MQ=255)
                                                                                gtcgCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCgg                >  1:1161646/1‑101 (MQ=255)
                                                                                gtcgCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCgg                >  2:2597267/1‑101 (MQ=255)
                                                                                gtcgCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCgg                >  2:4022102/1‑101 (MQ=255)
                                                                                 tcgCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGa               <  2:704008/101‑1 (MQ=255)
                                                                                  cgcgGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGAt              <  1:25114/101‑1 (MQ=255)
                                                                                   gcgGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATc             <  2:3147053/101‑1 (MQ=255)
                                                                                   gcgGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATc             >  1:243833/1‑101 (MQ=255)
                                                                                      ggCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCcac          <  2:1949860/101‑1 (MQ=255)
                                                                                      ggCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGCAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCcac          <  1:4044336/101‑1 (MQ=255)
                                                                                        cttcttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACAt        <  1:214295/101‑1 (MQ=255)
                                                                                        cttcttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACAt        <  2:3472944/101‑1 (MQ=255)
                                                                                          tcttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGt      <  1:3757117/101‑1 (MQ=255)
                                                                                           cttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTa     >  1:1994063/1‑101 (MQ=255)
                                                                                           cttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTa     <  1:4510423/101‑1 (MQ=255)
                                                                                           cttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTa     <  1:653601/101‑1 (MQ=255)
                                                                                            ttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACNTGTAt    >  1:1531192/1‑101 (MQ=255)
                                                                                            ttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTAt    <  2:2891012/101‑1 (MQ=255)
                                                                                            ttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTAt    <  2:2717781/101‑1 (MQ=255)
                                                                                             tGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTAtt   >  2:284078/1‑101 (MQ=255)
                                                                                              gCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTATTc  <  1:1896067/101‑1 (MQ=255)
                                                                                              gCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTAAGCTTCCTGCGCGGCGCTGGCGATATCCGCCGGATCCACATGTATTc  >  1:1314148/1‑101 (MQ=255)
                                                                                                    |                                                                                               
TAAGTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCTTTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTATTC  >  NC_014034/2027831‑2028026

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG