Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,217 Δ1 bp 100% coding (906/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,2170C→.100.0% 870.0 197coding (906/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (98/99):  ref base (0/0):  total (98/99)

Only 100 of 219 total aligned reads displayed.

CCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCT.GGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAA  >  NC_014034/2180120‑2180315
                                                                                                     |                                                                                                  
ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAc                                                                                                    <  2:4151450/101‑1 (MQ=255)
 cttccgatctgACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTATGGGCCTGGAAcc                                                                                                   <  1:3285917/91‑1 (MQ=255)
 cacccccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                                                                   <  2:170673/98‑1 (MQ=255)
 cANCCCCAAGGACTCGGNCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                                                                   <  2:910536/101‑1 (MQ=255)
   aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                                                                 <  2:53128/101‑3 (MQ=255)
   aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                                                                 <  1:4252102/101‑3 (MQ=255)
     cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                                                               <  2:2408076/101‑5 (MQ=255)
     cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                                                               >  2:1582486/1‑97 (MQ=255)
     cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                                                               <  1:4138439/101‑5 (MQ=255)
      ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                                                                             <  2:1451992/101‑1 (MQ=255)
        aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                                                           <  1:3546711/101‑2 (MQ=255)
        aaGAACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                                                           >  2:161821/1‑100 (MQ=255)
          ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                                                         >  2:1758658/1‑98 (MQ=255)
          ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                                                         <  1:2385236/101‑4 (MQ=255)
           gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                                                        <  2:1285087/101‑5 (MQ=255)
           gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTATGGGCCTGGAAccagatcggaag                                                                                         >  2:3285715/1‑91 (MQ=255)
            aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                                                      >  1:4454033/1‑101 (MQ=255)
            aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                                                      >  1:3412449/1‑101 (MQ=255)
               cGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattagatcg                                                                                    >  2:1343278/1‑93 (MQ=255)
                  ccGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                                                >  1:2540579/1‑101 (MQ=255)
                  ccGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                                                <  1:3766278/101‑1 (MQ=255)
                    gTGGCCGATCATGTCGGCTTCGCGCTGGGG.CCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                                                              >  2:1438911/1‑101 (MQ=255)
                    gTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                                                              <  1:1400896/101‑1 (MQ=255)
                     tGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                                                             >  2:2475028/1‑101 (MQ=255)
                     tGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                                                             <  2:3954470/101‑1 (MQ=255)
                        ccGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCCCGCaa                                                                          >  2:3638271/1‑101 (MQ=255)
                        ccGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                                                          <  2:3795540/101‑1 (MQ=255)
                        ccGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                                                          <  1:1758766/101‑1 (MQ=255)
                         cGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                                                         >  1:1275818/1‑101 (MQ=255)
                          gATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                        <  1:1300267/101‑1 (MQ=255)
                          gATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                        <  1:4240228/101‑1 (MQ=255)
                          gATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                        <  2:2946127/101‑1 (MQ=255)
                           aTCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                                                       <  2:726084/101‑2 (MQ=255)
                           aTCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                                                       >  2:429729/1‑100 (MQ=255)
                            tCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                                                                      >  2:1997181/1‑99 (MQ=255)
                             cATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                     >  1:948630/1‑98 (MQ=255)
                              aTGTCGGCTTCGCGCT.GGCGCGGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                    >  1:2690611/1‑97 (MQ=255)
                              aTGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                    >  1:309877/1‑97 (MQ=255)
                              aTGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                    >  1:4497651/1‑97 (MQ=255)
                              aTGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                    <  1:453845/101‑5 (MQ=255)
                               tGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                  <  2:574608/101‑1 (MQ=255)
                                gTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                                                 <  1:2708192/101‑1 (MQ=255)
                                gTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                                                 <  2:408876/101‑1 (MQ=255)
                                gTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                                                 <  1:4647620/101‑1 (MQ=255)
                                 tcggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                                                                <  1:2127497/101‑1 (MQ=255)
                                  cggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                               >  2:2219595/1‑101 (MQ=255)
                                  cggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                               >  1:1056282/1‑101 (MQ=255)
                                   ggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                              >  1:1123126/1‑101 (MQ=255)
                                   ggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                              <  1:1020304/101‑1 (MQ=255)
                                       tcgcgct.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                                                          <  1:2184475/97‑1 (MQ=255)
                                           gct.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                                                      <  1:2252475/101‑1 (MQ=255)
                                              tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                                                    <  1:3027486/96‑1 (MQ=255)
                                               ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                                                   >  2:752023/5‑101 (MQ=255)
                                               ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                                                   >  1:3192748/5‑101 (MQ=255)
                                                 cgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                                                 >  1:732353/3‑101 (MQ=255)
                                                  gccGAACAAGGTCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg                                                <  1:719268/100‑1 (MQ=255)
                                                   ccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCTGAAATtcgt                                               >  2:3685780/1‑101 (MQ=255)
                                                    cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                              <  2:3767664/101‑1 (MQ=255)
                                                    cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                              <  1:1974901/101‑1 (MQ=255)
                                                     gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                             >  2:1111508/1‑101 (MQ=255)
                                                      aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                            <  1:2748920/101‑1 (MQ=255)
                                                       aCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcgca                                           >  2:3080692/1‑99 (MQ=255)
                                                        cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt                                          >  1:1674022/1‑101 (MQ=255)
                                                         aaggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATg                                         >  2:3871856/6‑101 (MQ=255)
                                                          aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                        >  1:4564442/5‑101 (MQ=255)
                                                          aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                        <  2:1904999/97‑1 (MQ=255)
                                                           ggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATggg                                       >  2:3606748/4‑101 (MQ=255)
                                                             ccgggtgcgccAAGCATGGCAACTGGCGCTGGTCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg                                     <  1:3254826/91‑1 (MQ=255)
                                                             ccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg                                     <  2:4245430/100‑1 (MQ=255)
                                                              cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa                                    >  2:4415661/1‑101 (MQ=255)
                                                               ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                   >  2:3609498/1‑101 (MQ=255)
                                                               ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                   >  1:3714283/1‑101 (MQ=255)
                                                                 tgtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCt                                 <  1:3898163/95‑1 (MQ=255)
                                                                 tgtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCt                                 <  1:4438712/95‑1 (MQ=255)
                                                                 tgtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCt                                 <  1:2025882/95‑1 (MQ=255)
                                                                  gtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTc                                >  2:1676740/6‑101 (MQ=255)
                                                                   tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                               <  1:1582585/97‑1 (MQ=255)
                                                                    cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGGCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                              >  2:1521974/4‑101 (MQ=255)
                                                                    cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                              <  1:2488930/98‑1 (MQ=255)
                                                                    cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                              <  1:3607449/98‑1 (MQ=255)
                                                                     gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                             <  1:3925668/99‑1 (MQ=255)
                                                                      ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                            <  1:1446219/100‑1 (MQ=255)
                                                                        aaGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGa                          >  2:4573868/1‑101 (MQ=255)
                                                                         aGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGAc                         <  2:2611579/101‑1 (MQ=255)
                                                                           cATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTa                       <  2:4696915/101‑1 (MQ=255)
                                                                            aTGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTac                      <  1:1617335/101‑1 (MQ=255)
                                                                            aTGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTac                      >  2:229377/1‑101 (MQ=255)
                                                                             tGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGCCTaca                     >  2:3940778/1‑101 (MQ=255)
                                                                                     ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt             <  2:1605215/101‑1 (MQ=255)
                                                                                     ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt             <  2:3511032/101‑1 (MQ=255)
                                                                                     ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt             <  2:4608690/101‑1 (MQ=255)
                                                                                     ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt             >  1:4751503/1‑101 (MQ=255)
                                                                                     ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt             >  2:1190542/1‑101 (MQ=255)
                                                                                      gCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTg            <  2:1731805/101‑1 (MQ=255)
                                                                                       ctctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGt           <  1:638052/101‑1 (MQ=255)
                                                                                          tGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGc        >  2:3035184/1‑101 (MQ=255)
                                                                                           gggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGcc       <  2:2522680/101‑1 (MQ=255)
                                                                                            ggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCa      >  2:4029259/1‑101 (MQ=255)
                                                                                            ggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCa      <  2:2936699/101‑1 (MQ=255)
                                                                                                tGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaa  <  2:4506929/101‑1 (MQ=255)
                                                                                                     |                                                                                                  
CCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCT.GGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAA  >  NC_014034/2180120‑2180315

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG