Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,242 Δ1 bp 100% coding (931/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,2420A→.100.0% 763.7 172coding (931/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (77/95):  ref base (0/0):  total (77/95)

Only 100 of 199 total aligned reads displayed.

GCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGCCCC  >  NC_014034/2180143‑2180338
                                                                                                      |                                                                                                
gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                                                                                  <  1:693767/101‑1 (MQ=255)
   gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                                               <  2:2946127/101‑1 (MQ=255)
    aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                                                                              <  2:726084/101‑2 (MQ=255)
     tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                                                                                             >  2:1997181/1‑99 (MQ=255)
      cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                                            >  1:4619315/1‑98 (MQ=255)
      cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                                            >  1:948630/1‑98 (MQ=255)
      cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                                            <  2:223916/101‑4 (MQ=255)
       aTGTCGGCTTCGCGCTGGCGCGGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                                           >  1:2690611/1‑97 (MQ=255)
       aTGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                                           <  1:453845/101‑5 (MQ=255)
       aTGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                                           <  2:2983348/101‑5 (MQ=255)
       aTGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                                           >  2:3622341/1‑97 (MQ=255)
        tGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                                         >  1:1688752/1‑101 (MQ=255)
        tGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                                         <  2:574608/101‑1 (MQ=255)
         gTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                                                                        <  2:408876/101‑1 (MQ=255)
         gTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                                                                        <  1:2708192/101‑1 (MQ=255)
         gTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                                                                                        >  1:4507179/1‑101 (MQ=255)
           cggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                                                      >  1:315243/1‑101 (MQ=255)
           cggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                                                      >  1:1056282/1‑101 (MQ=255)
           cggattcgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                                                      >  2:395702/10‑101 (MQ=255)
            ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                                     >  1:2891336/1‑101 (MQ=255)
            ggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                                                                                     <  1:1020304/101‑1 (MQ=255)
               ttcgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcggc                                                                                  <  1:3622560/96‑1 (MQ=255)
                    gctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                                                                             >  2:978106/1‑101 (MQ=255)
                    gctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                                                                             <  1:2252475/101‑1 (MQ=255)
                      tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                                                                           <  1:3027486/96‑1 (MQ=255)
                       ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                                                                          >  2:752023/5‑101 (MQ=255)
                       ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                                                                          >  1:1842847/5‑101 (MQ=255)
                        gcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                                                                         <  2:3731041/98‑1 (MQ=255)
                         cgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                                                                        >  1:732353/3‑101 (MQ=255)
                          gccGAACAAGGTCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg                                                                       <  1:719268/100‑1 (MQ=255)
                            cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                                                     <  2:12513/101‑1 (MQ=255)
                            cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                                                     >  2:4233309/1‑101 (MQ=255)
                             gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                                                    >  2:1111508/1‑101 (MQ=255)
                               aCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcgca                                                                  >  2:3080692/1‑99 (MQ=255)
                               aCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCTGAAATTCGTCGaa                                                                  >  2:1285952/1‑101 (MQ=255)
                                cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt                                                                 >  1:1432061/1‑101 (MQ=255)
                                cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt                                                                 >  1:3511244/1‑101 (MQ=255)
                                 aaggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCCCTGAAATTCGTCGAATg                                                                >  2:3607230/6‑101 (MQ=255)
                                  aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                                               >  2:2903204/5‑101 (MQ=255)
                                  aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                                               <  2:1904999/97‑1 (MQ=255)
                                     ccgggtgcgccAAGCATGGCAACTGGCGCTGGTCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg                                                            <  1:3254826/91‑1 (MQ=255)
                                     ccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg                                                            >  1:4264592/2‑101 (MQ=255)
                                      cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa                                                           >  2:4415661/1‑101 (MQ=255)
                                      cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa                                                           >  1:1278713/1‑101 (MQ=255)
                                         tgtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCt                                                        >  1:1190630/7‑101 (MQ=255)
                                         tgtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCt                                                        <  1:4438712/95‑1 (MQ=255)
                                           tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                                                      <  1:1582585/97‑1 (MQ=255)
                                            cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGGCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                                     >  2:1521974/4‑101 (MQ=255)
                                            cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                                     <  1:3607449/98‑1 (MQ=255)
                                            cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                                     <  2:3368429/98‑1 (MQ=255)
                                            cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                                     <  1:2488930/98‑1 (MQ=255)
                                            cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                                                     <  1:161129/98‑1 (MQ=255)
                                             gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                                    <  1:4463532/99‑1 (MQ=255)
                                             gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                                    >  2:1446125/3‑101 (MQ=255)
                                             gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                                    <  2:2540434/99‑1 (MQ=255)
                                              ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                                   >  1:1669866/2‑101 (MQ=255)
                                              ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                                   <  1:1446219/100‑1 (MQ=255)
                                                aaGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGa                                                 >  2:4573868/1‑101 (MQ=255)
                                                  gCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACt                                               >  2:4284295/1‑101 (MQ=255)
                                                   cATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTa                                              <  2:3831562/101‑1 (MQ=255)
                                                   cATGGCAACTGGCTCTGGGCCCGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTa                                              <  2:4298405/101‑1 (MQ=255)
                                                     tGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGCCTaca                                            >  2:3940778/1‑101 (MQ=255)
                                                      ggCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTacac                                           <  2:315209/101‑1 (MQ=255)
                                                        cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCg                                         <  2:1686695/101‑1 (MQ=255)
                                                        cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGACGCGGCGCTGAAATTCGCGCAATGGCCGCCCTCCAAGCCCTacactt                                         >  1:2155471/1‑99 (MQ=255)
                                                          aCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGcc                                       >  1:4649985/1‑101 (MQ=255)
                                                            tGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCt                                     <  2:1842735/101‑1 (MQ=255)
                                                            tGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACGACACCGCCCt                                     >  1:33614/1‑101 (MQ=255)
                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                                    <  2:4608690/101‑1 (MQ=255)
                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                                    <  2:1605215/101‑1 (MQ=255)
                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                                    <  2:3511032/101‑1 (MQ=255)
                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                                    <  2:47145/101‑1 (MQ=255)
                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                                    >  1:4751503/1‑101 (MQ=255)
                                                              gCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTg                                   <  2:1731805/101‑1 (MQ=255)
                                                                 ctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCg                                <  2:3788657/101‑1 (MQ=255)
                                                                 ctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCg                                <  2:3557718/101‑1 (MQ=255)
                                                                   gggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGcc                              <  2:2522680/101‑1 (MQ=255)
                                                                   gggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGcc                              >  1:3737806/1‑101 (MQ=255)
                                                                   gggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGcc                              <  2:1654686/101‑1 (MQ=255)
                                                                    ggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCa                             <  2:2936699/101‑1 (MQ=255)
                                                                    ggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCa                             >  2:4029259/1‑101 (MQ=255)
                                                                    ggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCa                             <  1:1997298/101‑1 (MQ=255)
                                                                     gCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAg                            <  2:243786/101‑1 (MQ=255)
                                                                          ggaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaag                        <  2:2700683/97‑1 (MQ=255)
                                                                           gaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaagg                       >  1:3057479/4‑101 (MQ=255)
                                                                             accTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaaggaa                     >  2:477306/2‑101 (MQ=255)
                                                                             accTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaaggaa                     <  2:2323145/100‑1 (MQ=255)
                                                                              ccTCGCC.ATTCCGGCGGGCACAC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaaggaag                    <  2:3841252/101‑1 (MQ=255)
                                                                               cTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaaggaagg                   >  2:4626268/1‑101 (MQ=255)
                                                                                tCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGc                  >  1:1304571/1‑101 (MQ=255)
                                                                                tCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGc                  >  2:3661723/1‑101 (MQ=255)
                                                                                  cgccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCt                 >  2:803208/5‑101 (MQ=255)
                                                                                   gccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTg                <  2:4538897/98‑1 (MQ=255)
                                                                                    ccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTgg               >  2:1034443/3‑101 (MQ=255)
                                                                                     caTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTggg              >  1:401500/2‑101 (MQ=255)
                                                                                      aTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGc             >  2:4474446/1‑101 (MQ=255)
                                                                                      aTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGc             <  2:1190540/101‑1 (MQ=255)
                                                                                               ggCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGcc    >  1:637402/1‑101 (MQ=255)
                                                                                               ggCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGcc    >  1:3913664/1‑101 (MQ=255)
                                                                                                 cACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGcccc  <  1:2071892/101‑1 (MQ=255)
                                                                                                      |                                                                                                
GCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGCCCC  >  NC_014034/2180143‑2180338

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG