Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,185,902 Δ1 bp 100% intergenic (+175/‑330) mtlK → / → RCAP_rcc02028 mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,185,9020T→.100.0% 406.6 98intergenic (+175/‑330)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (38/59):  ref base (1/0):  total (39/59)

Only 100 of 109 total aligned reads displayed.

TTCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCG.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCGCCGGGTCTC  >  NC_014034/2185802‑2185988
                                                                                                     |                                                                                        
ttCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGt                                                                                          <  2:3002683/101‑1 (MQ=255)
 tCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGtt                                                                                         <  1:1098053/101‑1 (MQ=255)
   gCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGtttt                                                                                       <  2:4436471/101‑1 (MQ=255)
      tCGGCGGTGCCGAGGCGACCCTGCGGGGGTTCCG.CCCCCCGACCCCCGGCGTATTTATCCCAAGAAAAAGCCGCGGCTTTCCCCCCAAGACTTGttttgat                                                                                    >  2:2433192/1‑98 (MQ=25)
       cggcggTGCCGAGGCGCCCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttgatg                                                                                   <  2:4168416/101‑5 (MQ=37)
         gcggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                                                                                <  2:2369221/101‑1 (MQ=37)
         gcggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                                                                                <  2:1072827/101‑1 (MQ=37)
         gcggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                                                                                <  2:3757109/101‑1 (MQ=37)
         gcggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                                                                                <  1:2031705/101‑1 (MQ=37)
          cggTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGca                                                                               <  1:3785610/101‑1 (MQ=37)
            gTGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGcaca                                                                             <  1:150253/101‑1 (MQ=37)
             tGCCGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACaa                                                                            <  2:3453478/101‑1 (MQ=37)
               ccGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGa                                                                          <  2:3550323/101‑1 (MQ=37)
               ccGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGa                                                                          <  1:1271777/101‑1 (MQ=37)
                cgggccGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                         <  1:6243/96‑1 (MQ=25)
                cGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTTCCCCGAAGGGCTTG.TTTTGATGGCACAAGaa                                                                         >  2:366452/1‑101 (MQ=25)
                cGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                         <  2:1957172/101‑1 (MQ=37)
                cGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                         <  1:3757199/101‑1 (MQ=37)
                cGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                         <  1:2720896/101‑1 (MQ=37)
                cGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                         <  1:1133577/101‑1 (MQ=37)
                cGAGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                         <  1:145079/101‑1 (MQ=37)
                  aGGCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTT.ATGGCACAAAAAAc                                                                       >  1:1673945/1‑101 (MQ=25)
                  aGGCGACCCTGCGGGGGCTCCG.CCCCCGCTCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                                                                       <  1:1536581/101‑1 (MQ=37)
                   ggCGACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACg                                                                      <  2:4201838/101‑1 (MQ=37)
                   ggCGACCCTGCGGGGGCTCCG.CCCCCGCCCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACg                                                                      <  2:4295714/101‑1 (MQ=37)
                      gACCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCCAAGGCCGTG.TTTTGATGGCACAAGAAACGCCg                                                                   >  2:2572122/1‑101 (MQ=25)
                       aCCCTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGa                                                                  <  1:2835365/101‑1 (MQ=37)
                         ccTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.Ac                                                               <  1:1285674/101‑1 (MQ=37)
                          cTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.Acc                                                              <  1:4276087/101‑1 (MQ=37)
                          cTGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.Acc                                                              <  1:3076012/101‑1 (MQ=37)
                           tGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCg                                                             <  2:4204848/101‑1 (MQ=37)
                             cGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCG.cc                                                          <  2:3452597/101‑1 (MQ=37)
                                  gCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCctt                                                     <  2:2433821/101‑1 (MQ=37)
                                  gCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCctt                                                     <  2:3493932/101‑1 (MQ=37)
                                  gCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCctt                                                     <  1:1979636/101‑1 (MQ=37)
                                   cTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCcttt                                                    <  1:1960695/101‑2 (MQ=37)
                                   cTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCcttt                                                    <  2:4077923/101‑2 (MQ=37)
                                    tccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCctttc                                                   >  1:3418966/6‑99 (MQ=37)
                                    tccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCctttc                                                   <  2:274819/96‑3 (MQ=37)
                                      cgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCAc                                                  <  1:3691128/98‑1 (MQ=38)
                                      cgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCAc                                                  >  1:2831382/4‑101 (MQ=38)
                                       gcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCCCAAGAAACGCCGA.ACCGCCCCCTTTCAcc                                                 >  1:3387795/3‑101 (MQ=38)
                                        cccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCt                                                <  1:3415297/100‑1 (MQ=38)
                                        cccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCt                                                <  2:3250880/100‑1 (MQ=38)
                                         ccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCtt                                               <  2:3947075/101‑1 (MQ=38)
                                          cccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGGACAAGAAACGCCGA.ACCGCCCCCTTTCCCCTTg                                              >  1:4051642/1‑101 (MQ=255)
                                          cccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTg                                              >  1:3712301/1‑101 (MQ=38)
                                           cccgcgcccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTg                                              <  1:1602509/95‑1 (MQ=38)
                                             cgcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTT.ATGGCACAAAAAAAGCCGA.ACCGCCCCCTTTCACCCTGGc                                            >  2:3666089/5‑101 (MQ=255)
                                             cgcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGc                                            >  2:3370271/5‑101 (MQ=255)
                                              gcacccccGGCGTATTTATCCCAAGAAAAAGCATCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCt                                           >  1:2030297/4‑101 (MQ=255)
                                              gcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCt                                           >  1:1829755/4‑101 (MQ=255)
                                              gcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCt                                           >  1:3013979/4‑101 (MQ=255)
                                               cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTTCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGTCTc                                          >  1:1387385/3‑101 (MQ=38)
                                               cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTc                                          >  1:3602962/3‑101 (MQ=255)
                                                 cccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCaa                                        <  1:3957476/101‑1 (MQ=255)
                                                 cccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCaa                                        >  1:3731836/1‑101 (MQ=255)
                                                  ccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCaaa                                       <  1:3370477/101‑1 (MQ=255)
                                                  ccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCaaa                                       >  2:1173591/1‑101 (MQ=255)
                                                   cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAat                                      <  2:3087042/101‑1 (MQ=255)
                                                   cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAat                                      <  1:2398340/101‑1 (MQ=255)
                                                     cGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAatat                                    <  1:2957452/101‑1 (MQ=255)
                                                      ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATc                                   >  2:1590716/1‑101 (MQ=255)
                                                      ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATc                                   >  2:1579731/1‑101 (MQ=255)
                                                      ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATc                                   <  2:1314124/101‑1 (MQ=255)
                                                       gCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATcc                                  >  1:4748640/1‑101 (MQ=255)
                                                        cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATccc                                 <  1:3319079/101‑1 (MQ=255)
                                                        cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATccc                                 <  2:546385/101‑1 (MQ=255)
                                                         gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCg                                <  2:4506427/101‑1 (MQ=255)
                                                         gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCg                                >  1:942191/1‑101 (MQ=255)
                                                         gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCg                                >  1:420665/1‑101 (MQ=255)
                                                          tATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCgg                               >  2:638831/1‑101 (MQ=255)
                                                          tATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCgg                               >  2:3443214/1‑101 (MQ=255)
                                                           aTTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCggg                              <  1:1579831/101‑1 (MQ=255)
                                                           aTTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCggg                              >  2:255395/1‑101 (MQ=255)
                                                             ctATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCggggg                            <  2:1526171/100‑1 (MQ=39)
                                                              tATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGt                           >  1:2390317/1‑101 (MQ=255)
                                                              tATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGt                           >  1:3030817/1‑101 (MQ=255)
                                                              tATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGt                           >  1:4350656/1‑101 (MQ=255)
                                                               aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCCCCTTGGCTCAAATATCCCggggggg                          >  1:1526272/1‑99 (MQ=255)
                                                               aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTc                          <  1:3817493/101‑1 (MQ=255)
                                                                tCCCAAGAAAAAGCATCGGCATGCCCCGAAGGGCTTGTTTTT.ATGGGACAAGAAAAGCCAA.ACAGCCCCCCCTCACCTTGGCTCAAATATCCCGGGGGTcc                         >  2:330770/1‑101 (MQ=19)
                                                                tCCCAAGAAAAAGCAGCGGCTTTCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCGGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         <  2:3679886/101‑1 (MQ=38)
                                                                tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         <  2:2831217/101‑1 (MQ=255)
                                                                tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         <  2:3598873/101‑1 (MQ=255)
                                                                 cccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                        >  2:2994983/1‑101 (MQ=255)
                                                                    aaGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCgggg                     <  1:2465401/101‑1 (MQ=255)
                                                                    aaGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCgggg                     >  1:199110/1‑101 (MQ=255)
                                                                       aaaaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCg                  >  2:2921070/1‑101 (MQ=255)
                                                                       aaaaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGGCCGGGGGCg                  >  2:124648/1‑101 (MQ=38)
                                                                        aaaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCgg                 >  2:4312070/1‑101 (MQ=255)
                                                                         aaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCggc                >  2:2003376/1‑100 (MQ=38)
                                                                              agcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccc           <  1:664908/101‑2 (MQ=37)
                                                                              agcGGCTTGCCCCGAAAGACTTG.TTTTGATGGCACAAGAAATACCCA.AACGCCCCCTTTCACCTTTGCTCAAAAATGCCGGGGGGACGGGGGCGGAgggcc           >  2:1992097/1‑97 (MQ=255)
                                                                               tcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccccc          <  2:2030180/100‑3 (MQ=37)
                                                                                cGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccg         <  1:3912014/101‑4 (MQ=37)
                                                                                    ttGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcgg     >  1:4522225/1‑94 (MQ=25)
                                                                                     tGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcggg    >  1:2443020/1‑93 (MQ=21)
                                                                                      cccccGAATGCCTTG.TTTTGATGGCACAAGAAACCCCGACCCCC.CCCCTTTCCCCTGGGCTAAAATTCCCGGGGGGTCGGGGGGCGGAGcccccggcgggt   <  2:3418760/100‑10 (MQ=12)
                                                                                       ccccGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcggggg  >  2:1550205/1‑91 (MQ=21)
                                                                                                     |                                                                                        
TTCGCCTCGGCGGTGCCGAGGCGACCCTGCGGGGGCTCCG.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCGCCGGGTCTC  >  NC_014034/2185802‑2185988

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG