Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,198,718 Δ1 bp 97.6% coding (151/729 nt) cobA2 ← uroporphyrinogen‑III C‑methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,198,7180C→.97.6% 5.5 ‑1.0 ‑0.1169coding (151/729 nt)cobA2uroporphyrinogen‑III C‑methyltransferase
Reads supporting (aligned to +/- strand):  new base (86/79):  ref base (1/3):  total (87/82)
Fisher's exact test strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.69e-01

Only 100 of 191 total aligned reads displayed.

ACCCGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGCGGGCATGGCCCCAGGATCGGCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCA  >  NC_014034/2198618‑2198816
                                                                                                    |                                                                                                  
accCGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCCCGATGAGTTTGGc                                                                                                    >  1:4253520/1‑101 (MQ=255)
 cccGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGcc                                                                                                   <  2:2133759/101‑1 (MQ=255)
    gTTGCGCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGccccc                                                                                                <  1:1851864/101‑1 (MQ=255)
    gTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGccccc                                                                                                >  2:3773153/1‑101 (MQ=255)
     ttGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGcccccg                                                                                               >  1:4443401/1‑100 (MQ=255)
      tGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGcccccgg                                                                                              <  2:441715/101‑3 (MQ=255)
      tGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGcccccgg                                                                                              >  1:2921578/1‑99 (MQ=255)
        cccGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCgggc                                                                                            <  2:3994825/101‑2 (MQ=255)
        cccGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGCCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCgggc                                                                                            >  1:1461358/1‑100 (MQ=255)
           ggTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCcgggcggg                                                                                        >  2:741417/1‑101 (MQ=255)
            gTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCgggcgggc                                                                                       >  2:938728/1‑101 (MQ=255)
             tCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGCTGAGTTCGG.CCCCCgggcgggcc                                                                                      >  1:2058721/1‑100 (MQ=255)
             tCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCa                                                                                      >  2:4481656/1‑101 (MQ=255)
              cAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCAt                                                                                     >  1:3336592/1‑101 (MQ=255)
                  gcATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcc                                                                                 <  2:3205989/101‑1 (MQ=255)
                   cATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccc                                                                                >  2:4250558/1‑101 (MQ=255)
                    aTGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGccca                                                                               >  1:3740733/1‑100 (MQ=255)
                       aTCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcccagga                                                                            <  1:741491/101‑5 (MQ=255)
                       aTCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGGcccagga                                                                            >  1:2715841/1‑97 (MQ=255)
                         cgacgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCCTGG.CCCAGGATc                                                                         >  2:1459893/1‑101 (MQ=255)
                         cgacgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATc                                                                         <  1:2044361/101‑1 (MQ=255)
                          gacgaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCg                                                                        <  1:287837/101‑1 (MQ=255)
                             gaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcc                                                                     >  1:1194076/1‑101 (MQ=255)
                              aGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGccc                                                                    >  2:1027678/1‑101 (MQ=255)
                              aGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGccc                                                                    <  1:2451131/101‑1 (MQ=255)
                              aGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGccc                                                                    <  2:1650610/101‑1 (MQ=255)
                               gCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccg                                                                   <  1:4166839/101‑2 (MQ=255)
                               gCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccg                                                                   <  1:3771030/101‑2 (MQ=255)
                                cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGCCCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                  >  2:445904/1‑99 (MQ=255)
                                cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                  >  1:174597/1‑99 (MQ=255)
                                cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                  >  2:2475660/1‑99 (MQ=255)
                                 aaGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccggc                                                                 <  1:2323470/101‑4 (MQ=255)
                                 aaGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccggc                                                                 >  2:1622867/1‑98 (MQ=255)
                                  aGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccggcg                                                                >  1:3473770/1‑97 (MQ=255)
                                    cGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGa                                                             >  2:4550619/1‑101 (MQ=255)
                                    cGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGa                                                             >  2:505278/1‑101 (MQ=255)
                                    cGGCCCACCTCATCCTGCTTCGGCGAGGACCGCCCTTCCCTCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGa                                                             <  2:1770164/101‑1 (MQ=255)
                                     ggCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaa                                                            >  1:1545333/1‑101 (MQ=255)
                                     ggCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaa                                                            <  1:1062223/101‑1 (MQ=255)
                                       cTCACATGATCCTGCGTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.GCCGGCGGAAAg                                                          <  1:3919849/101‑1 (MQ=255)
                                          acaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtg                                                       >  1:3126862/1‑100 (MQ=255)
                                          acaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtc                                                       <  1:1907951/101‑1 (MQ=255)
                                          acaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGCGGGGCA.GGGCCCGGGAT.GGGCCCGGGGGAAAAAtg                                                       >  2:3280729/1‑100 (MQ=255)
                                           caTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcg                                                      >  2:2381433/1‑101 (MQ=255)
                                            aTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgt                                                     >  1:848711/1‑101 (MQ=255)
                                             tGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgtc                                                    <  2:279610/101‑1 (MQ=255)
                                             tGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgtc                                                    <  1:2816962/101‑1 (MQ=255)
                                              gATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgtcg                                                   <  2:4378176/101‑1 (MQ=255)
                                              gATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgtcg                                                   >  2:2996189/1‑101 (MQ=255)
                                               aTCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGa                                                  <  2:4450672/101‑1 (MQ=255)
                                               aTCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGa                                                  >  1:1089326/1‑101 (MQ=255)
                                               aTCCTGCTTCGGCGAGGGCCGCCCCGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGa                                                  <  1:3937675/101‑1 (MQ=255)
                                                  ccggttCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACa                                               <  2:1932433/97‑1 (MQ=255)
                                                      ttCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcac                                           >  2:1888803/1‑101 (MQ=255)
                                                         ggCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGGGGAAAGAGCGGCGAACACcacgcc                                        >  2:3092402/1‑98 (MQ=255)
                                                         ggCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcaccac                                        <  2:3736626/101‑1 (MQ=255)
                                                         ggCGAGGGCCCCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcaccac                                        <  2:1725196/101‑1 (MQ=255)
                                                           cGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACAt                                      >  1:4331642/1‑101 (MQ=255)
                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     <  2:3525847/101‑1 (MQ=255)
                                                            gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                     >  2:3861649/1‑101 (MQ=255)
                                                             aGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCg                                    <  2:1674315/101‑1 (MQ=255)
                                                             aGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCg                                    <  2:2495788/101‑1 (MQ=255)
                                                             aGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCg                                    >  2:407296/1‑101 (MQ=255)
                                                               ggCCGCCCGGCCCGCTTGCCGACCGCGATGCGTTCGG.CCCCCGGGCGAGCATGG.CCCAAGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGc                                  <  2:2487871/101‑1 (MQ=255)
                                                               ggCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGc                                  >  2:3857665/1‑101 (MQ=255)
                                                               ggCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGc                                  <  1:2285212/101‑1 (MQ=255)
                                                                gccgccCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGcc                                 >  2:140013/1‑101 (MQ=255)
                                                                 ccgccCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCt                                >  1:4011912/1‑101 (MQ=255)
                                                                 ccgccCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCt                                <  1:1382941/101‑1 (MQ=255)
                                                                   ggccggcccgGTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTg                              <  2:695984/99‑1 (MQ=255)
                                                                   gcccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGCACAGCACCACATCGGCCTTg                              >  2:571164/1‑101 (MQ=255)
                                                                   gcccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACGACATCGGCCTTg                              <  1:2577996/101‑1 (MQ=255)
                                                                   gcccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTg                              <  2:1193986/101‑1 (MQ=255)
                                                                      cggcccgCTTGCCGACCGCGATGCGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCAAATCGGCCTTGGCg                           >  2:4740117/1‑101 (MQ=255)
                                                                      cggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCg                           <  2:1965903/101‑1 (MQ=255)
                                                                       ggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGa                          <  1:379765/101‑1 (MQ=255)
                                                                          ccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGcc                       <  1:688078/101‑1 (MQ=255)
                                                                            gCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCgg                     >  1:3812909/1‑101 (MQ=255)
                                                                              ttGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTc                   >  1:1374636/1‑101 (MQ=255)
                                                                              ttGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTc                   <  2:4718226/101‑1 (MQ=255)
                                                                               tGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCg                  <  1:1017725/101‑1 (MQ=255)
                                                                                 ccGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGc                <  1:1281196/101‑1 (MQ=255)
                                                                                 ccGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGc                <  2:127538/101‑1 (MQ=255)
                                                                                  cGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCa               >  2:2631889/1‑101 (MQ=255)
                                                                                     ccGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCCCCACATCGGCCTTGGCGAGCCGGTCGGCAGCt            <  1:3053622/101‑1 (MQ=255)
                                                                                     ccGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCt            >  1:165859/1‑101 (MQ=255)
                                                                                        cgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTc         <  2:3041532/101‑1 (MQ=255)
                                                                                        cgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTc         <  2:1716877/101‑1 (MQ=255)
                                                                                        cgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTc         <  1:3639243/101‑1 (MQ=255)
                                                                                            gAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCg     >  1:3040585/1‑101 (MQ=255)
                                                                                            gAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCg     >  1:540636/1‑101 (MQ=255)
                                                                                             aGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGt    >  1:490766/1‑101 (MQ=255)
                                                                                             aGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGt    >  2:4173247/1‑101 (MQ=255)
                                                                                              gTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTc   <  1:2865138/101‑1 (MQ=255)
                                                                                              gTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTc   <  2:2499213/101‑1 (MQ=255)
                                                                                              gTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCAAGCCGGGCGGCAGCTTTCAGCGTc   >  1:995770/1‑101 (MQ=255)
                                                                                               ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa  >  2:1217444/1‑101 (MQ=255)
                                                                                               ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa  <  2:53828/101‑1 (MQ=255)
                                                                                               ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa  >  1:1977209/1‑101 (MQ=255)
                                                                                               ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa  <  2:3336390/101‑1 (MQ=255)
                                                                                                    |                                                                                                  
ACCCGTTGCCCGGTCAGCGCATGATCGACGAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGCGGGCATGGCCCCAGGATCGGCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCA  >  NC_014034/2198618‑2198816

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG