Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,198,748 Δ1 bp 100% coding (121/729 nt) cobA2 ← uroporphyrinogen‑III C‑methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,198,7480C→.100.0% 688.7 156coding (121/729 nt)cobA2uroporphyrinogen‑III C‑methyltransferase
Reads supporting (aligned to +/- strand):  new base (74/82):  ref base (0/0):  total (74/82)

Only 100 of 182 total aligned reads displayed.

GAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGCGGGCATGGCCCCAGGATCGGCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCGGGCCCGAGGAG  >  NC_014034/2198647‑2198845
                                                                                                     |                                                                                                 
gaGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcc                                                                                                  >  1:1194076/1‑101 (MQ=255)
 aGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGccc                                                                                                 <  1:2451131/101‑1 (MQ=255)
  gCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccg                                                                                                >  2:4645125/1‑100 (MQ=255)
  gCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccg                                                                                                <  1:3771030/101‑2 (MQ=255)
  gCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccg                                                                                                <  1:4166839/101‑2 (MQ=255)
   cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGCCCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                                               >  2:445904/1‑99 (MQ=255)
   cAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccgg                                                                                               >  1:174597/1‑99 (MQ=255)
    aaGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccggc                                                                                              >  2:1622867/1‑98 (MQ=255)
    aaGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGGcccggc                                                                                              >  1:3736861/1‑98 (MQ=255)
       cGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGa                                                                                          >  2:505278/1‑101 (MQ=255)
       cGGCCCACCTCATCCTGCTTCGGCGAGGACCGCCCTTCCCTCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGa                                                                                          <  2:1770164/101‑1 (MQ=255)
        ggCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaa                                                                                         >  1:1545333/1‑101 (MQ=255)
        ggCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaa                                                                                         <  1:1062223/101‑1 (MQ=255)
         gCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGaaa                                                                                        >  1:2806800/1‑101 (MQ=255)
            cacaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCGTGG.CCCAGGATCGG.CCCGGCGGAAAGAt                                                                                     >  1:1291479/1‑101 (MQ=255)
             acaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtc                                                                                    <  1:1907951/101‑1 (MQ=255)
             acaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtc                                                                                    <  2:1641139/101‑1 (MQ=255)
             acaTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGCGGGGCA.GGGCCCGGGAT.GGGCCCGGGGGAAAAAtg                                                                                    >  2:3280729/1‑100 (MQ=255)
              caTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcg                                                                                   >  2:2381433/1‑101 (MQ=255)
              caTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcg                                                                                   >  1:3526059/1‑101 (MQ=255)
               aTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgt                                                                                  >  2:2721364/1‑101 (MQ=255)
               aTGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGAtcgt                                                                                  >  1:848711/1‑101 (MQ=255)
                  aTCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGa                                                                               <  2:4450672/101‑1 (MQ=255)
                  aTCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGa                                                                               >  1:1089326/1‑101 (MQ=255)
                  aTCCTGCTTCGGCGAGGGCCGCCCCGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGa                                                                               <  1:3937675/101‑1 (MQ=255)
                    ccTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGCAc                                                                             >  1:1233633/1‑101 (MQ=255)
                     ccggttCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACa                                                                            <  2:1932433/97‑1 (MQ=255)
                     cTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACa                                                                            <  2:1029288/101‑1 (MQ=255)
                        cTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGca                                                                         >  2:4533098/1‑101 (MQ=255)
                           cGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcacca                                                                      >  1:2055209/1‑101 (MQ=255)
                            ggCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGGGGAAAGAGCGGCGAACACcacgcc                                                                     >  2:3092402/1‑98 (MQ=255)
                            ggCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcaccac                                                                     >  1:3084724/1‑101 (MQ=255)
                            ggCGAGGGCCCCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGcaccac                                                                     <  2:1725196/101‑1 (MQ=255)
                              cGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACAt                                                                   <  1:285169/101‑1 (MQ=255)
                               gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                                                  <  2:3525847/101‑1 (MQ=255)
                               gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                                                  <  2:1428534/101‑1 (MQ=255)
                               gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                                                  >  2:3861649/1‑101 (MQ=255)
                               gAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                                                  >  2:434676/1‑101 (MQ=255)
                               gAGCGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATc                                                                  <  1:3342360/101‑1 (MQ=255)
                                aGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCg                                                                 >  2:407296/1‑101 (MQ=255)
                                aGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCg                                                                 <  2:1674315/101‑1 (MQ=255)
                                  ggCCGCCCGGCCCGCTTGCCGACCGCGATGCGTTCGG.CCCCCGGGCGAGCATGG.CCCAAGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGc                                                               <  2:2487871/101‑1 (MQ=255)
                                  ggCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGc                                                               >  2:3857665/1‑101 (MQ=255)
                                    ccgccCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCt                                                             >  1:4011912/1‑101 (MQ=255)
                                    ccgccCGGCCCGCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCt                                                             <  1:1382941/101‑1 (MQ=255)
                                      ggccggcccgGTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTg                                                           <  2:695984/99‑1 (MQ=255)
                                      gcccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGCACAGCACCACATCGGCCTTg                                                           >  2:571164/1‑101 (MQ=255)
                                      gcccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACGACATCGGCCTTg                                                           <  1:2577996/101‑1 (MQ=255)
                                        ccggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGc                                                         <  1:147213/101‑1 (MQ=255)
                                         cggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCg                                                        <  2:1965903/101‑1 (MQ=255)
                                          ggcccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGa                                                       <  1:379765/101‑1 (MQ=255)
                                             ccgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGcc                                                    <  1:688078/101‑1 (MQ=255)
                                              cgCTTGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCg                                                   <  2:640282/101‑1 (MQ=255)
                                              cgCTGGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCg                                                   <  2:4723353/101‑1 (MQ=255)
                                                  tGCCGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCg                                               <  1:1017725/101‑1 (MQ=255)
                                                    ccGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGc                                             <  2:127538/101‑1 (MQ=255)
                                                    ccGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGc                                             >  1:4274071/1‑101 (MQ=255)
                                                    ccGACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGc                                             <  1:2285220/101‑1 (MQ=255)
                                                      gACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCCCCACATCGGCCTTGGCGAGCCGGTCGGCAg                                           <  1:3257659/101‑1 (MQ=255)
                                                      gACCGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAg                                           >  2:2580923/1‑101 (MQ=255)
                                                        ccGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCCCCACATCGGCCTTGGCGAGCCGGTCGGCAGCt                                         <  1:3053622/101‑1 (MQ=255)
                                                        ccGCGATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCt                                         >  1:165859/1‑101 (MQ=255)
                                                         cgcgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCtt                                        >  1:2407433/1‑101 (MQ=255)
                                                          gcgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCttt                                       >  2:1214701/1‑101 (MQ=255)
                                                           ggATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGGCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTc                                      <  1:521207/100‑1 (MQ=255)
                                                           cgATGAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTc                                      <  2:3041532/101‑1 (MQ=255)
                                                               gAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCg                                  >  1:3040585/1‑101 (MQ=255)
                                                               gAGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCg                                  >  1:540636/1‑101 (MQ=255)
                                                                aGTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGt                                 >  2:4173247/1‑101 (MQ=255)
                                                                 gTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTc                                <  1:2865138/101‑1 (MQ=255)
                                                                 gTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTc                                <  2:2499213/101‑1 (MQ=255)
                                                                 gTTCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCAAGCCGGGCGGCAGCTTTCAGCGTc                                >  1:995770/1‑101 (MQ=255)
                                                                  ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa                               <  2:4381890/101‑1 (MQ=255)
                                                                  ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa                               <  2:53828/101‑1 (MQ=255)
                                                                  ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa                               <  2:3336390/101‑1 (MQ=255)
                                                                  ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa                               >  1:1977209/1‑101 (MQ=255)
                                                                  ttCGG.CCCCCGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCa                               >  2:1217444/1‑101 (MQ=255)
                                                                    tcggcccccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAg                              <  2:4308274/97‑1 (MQ=255)
                                                                     gggcccccGGGCGGGCATGG.CCCAGGATCGG.CCCGGGGGAAAGATCGTCGAACAGCCCCACATCGGCCTGGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGa                             <  1:4231383/98‑1 (MQ=255)
                                                                       gcccccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCCCCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAg                           <  2:3912845/100‑1 (MQ=255)
                                                                          cccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTc                        >  1:2927767/1‑101 (MQ=255)
                                                                          cccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTc                        <  1:4481906/101‑1 (MQ=255)
                                                                           ccGGGCGGGCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTcc                       <  1:3860889/101‑1 (MQ=255)
                                                                            cgggcgggCATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAAAAGCACCCCATCGGACTTGGCGAGCCGGTCGGCAGAGTTAAGCGTCAGAAGCTCCg                      >  1:4729239/1‑101 (MQ=255)
                                                                               gcgggcATGG.CCCGGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGAt                   <  2:2950279/101‑1 (MQ=255)
                                                                                 gggcATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCg                 >  2:1964189/1‑101 (MQ=255)
                                                                                 gggcATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCg                 <  1:40455/101‑1 (MQ=255)
                                                                                 gggcAT.GCCCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCg                 <  2:1372243/101‑1 (MQ=255)
                                                                                    cATGG.CCCAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGccc              >  2:3198585/1‑101 (MQ=255)
                                                                                      atggcccAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCg             >  1:2484175/5‑101 (MQ=255)
                                                                                      atggcccAGGATCGG.CCAGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCg             <  2:3473558/97‑1 (MQ=255)
                                                                                        ggcccAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCggg           >  1:4336467/3‑101 (MQ=255)
                                                                                         gcccAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCGGGc          >  2:4705839/2‑101 (MQ=255)
                                                                                            cAGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCGGGCCCg       >  1:3904145/1‑101 (MQ=255)
                                                                                             aGGATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCGGGCCCga      >  2:746231/1‑101 (MQ=255)
                                                                                              ggATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCGGGCCCgag     >  1:1326256/1‑101 (MQ=255)
                                                                                               gATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCGGGCCCgagg    >  2:3605128/1‑101 (MQ=255)
                                                                                               gATCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCGGGCCCgagg    >  2:318158/1‑101 (MQ=255)
                                                                                                aTCGG.CCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCGGGCCCgagga   <  2:3743813/101‑1 (MQ=255)
                                                                                                  tcgCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCGGGCCCgaggag  <  2:2290244/99‑1 (MQ=255)
                                                                                                     |                                                                                                 
GAGCAAGCGGCTCACATGATCCTGCTTCGGCGAGGGCCGCCCGGCCCGCTTGCCGACCGCGATGAGTTCGGCCCCCCGGGCGGGCATGGCCCCAGGATCGGCCCCGGCGGAAAGATCGTCGAACAGCACCACATCGGCCTTGGCGAGCCGGTCGGCAGCTTTCAGCGTCAGAAGCTCCGGATCGCCCGGGCCCGAGGAG  >  NC_014034/2198647‑2198845

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG