Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,358 Δ1 bp 2.5% intergenic (‑154/‑151) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3580G→. 2.5% 7.3 0.0 ‑0.3200intergenic (‑154/‑151)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (98/96):  total (102/98)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.31e-01

Only 100 of 226 total aligned reads displayed.

CCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.C.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACC  >  NC_014034/2249259‑2249457
                                                                                                           |                                                                                                           
ccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                                             >  2:1646760/1‑101 (MQ=255)
ccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                                             <  2:3666721/101‑1 (MQ=255)
ccGGGGCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                                                             <  1:2174623/101‑1 (MQ=255)
  ggggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                                                           <  2:2131654/101‑1 (MQ=255)
  ggggCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                                                           <  1:909655/101‑1 (MQ=255)
     gCAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggg                                                                                                        <  2:219761/101‑1 (MQ=255)
      cAAGAAGGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                                                       <  1:4423044/101‑1 (MQ=255)
       aagaagGGGGCTCTAC.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                                                      >  2:4733758/1‑99 (MQ=255)
       aagaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                                                      <  1:4301777/101‑1 (MQ=255)
        agaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGa                                                                                                     >  2:4347125/1‑101 (MQ=255)
         gaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                                    <  1:3706845/101‑1 (MQ=255)
         gaagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                                                    <  1:2897477/101‑1 (MQ=255)
          aagGGGGCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaag                                                                                                   >  1:1350309/1‑100 (MQ=255)
           agGCGGCTCT.CCCCCCGCCCCGTCCCGGGCCC.CCCCCGGTATTTTGGAGGCAAGATGAG...GGGG..GCCTTTCTCCTTGCCAAAAATATCCCGGGGGGTGAA.Tg                                                                                                 <  2:883553/101‑1 (MQ=16)
             ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                               <  1:1297624/101‑1 (MQ=255)
             ggggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                                               >  2:3586307/1‑101 (MQ=255)
              gggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcg                                                                                              >  1:4157999/1‑101 (MQ=255)
               ggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                                             <  1:110420/101‑1 (MQ=255)
               ggCTCT.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                                                             <  2:482480/101‑1 (MQ=255)
                 ctct.GCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                                                         <  2:3472424/101‑1 (MQ=255)
                   tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                                                        >  1:264106/6‑101 (MQ=255)
                   tctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gc                                                                                        >  1:999976/6‑101 (MQ=255)
                    ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                                                       <  1:3605719/97‑1 (MQ=255)
                    ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGAAAGATGAA...GGGG..GGCTTTCATCTTGCCG.AAATATCCCCGGGGGGGAA.TGGCGCA..GCCa                                                                                      >  1:3928765/5‑101 (MQ=37)
                       cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                                                    <  2:1794228/100‑1 (MQ=255)
                          ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCAGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                                                 <  2:1978608/101‑1 (MQ=255)
                           cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                                >  2:45967/1‑101 (MQ=255)
                           cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                                >  1:4450149/1‑101 (MQ=255)
                           cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                                                                >  2:81113/1‑101 (MQ=255)
                            gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                                               <  2:1738376/101‑1 (MQ=255)
                              cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                                                             >  2:4294425/1‑101 (MQ=255)
                               ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                            >  2:4528299/1‑101 (MQ=255)
                               ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                                                            <  2:3214748/101‑1 (MQ=255)
                                cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                           >  1:296729/1‑101 (MQ=255)
                                cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                           >  2:2785752/1‑101 (MQ=255)
                                cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                           >  1:1718570/1‑101 (MQ=255)
                                 gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGTAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                                         >  2:4617883/1‑101 (MQ=255)
                                 gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                                                              <  1:3576625/95‑1 (MQ=18)
                                  tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cc                                                                        >  2:1468707/1‑100 (MQ=255)
                                   gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg.c                                                                      >  1:233057/1‑101 (MQ=255)
                                    ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c                                                                    >  1:3550859/1‑101 (MQ=255)
                                     gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.c                                                                         <  1:991578/99‑1 (MQ=18)
                                       ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.cg.c                                                                      <  1:2744523/101‑1 (MQ=17)
                                        gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.c.ccc                                                              <  1:737818/101‑2 (MQ=255)
                                         gcT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.c.ccct                                                             <  2:245395/100‑3 (MQ=255)
                                          cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.c.ccctt                                                            <  1:3089351/101‑4 (MQ=255)
                                          cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.c.ccctt                                                            <  1:4020275/101‑4 (MQ=255)
                                           t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.c.cccttc                                                           <  2:3059914/101‑5 (MQ=255)
                                           t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.c.cccttc                                                           <  1:1557905/101‑5 (MQ=255)
                                            gcccccGGGATATTTGGGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.c.cccttc                                                           <  1:4347372/100‑5 (MQ=255)
                                             cccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.C.C.C.CCt                                                              <  2:3851935/101‑1 (MQ=0)
                                             cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.c.cccttcg                                                          <  1:4528552/101‑6 (MQ=255)
                                             cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.c.cccttcg                                                          >  1:812051/1‑96 (MQ=255)
                                             cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.c.c.c.cccttcg                                                          <  2:677650/101‑6 (MQ=255)
                                               cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTcgcg                                                         <  2:1213634/101‑1 (MQ=255)
                                                ccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCgcgg                                                        <  2:607886/101‑1 (MQ=255)
                                                 cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.C.C.C.CCtgccc                                                          >  2:2731666/1‑97 (MQ=0)
                                                 cGGGATATTTGAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCgcggg                                                       <  2:180712/101‑1 (MQ=255)
                                                   ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCgcgggcg                                                     >  1:2113736/1‑101 (MQ=255)
                                                    gATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCgcgggcgg                                                    >  2:3999898/1‑101 (MQ=255)
                                                      tatTTGAGGCAAGATGAG...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGcc                                                  <  2:3019066/101‑1 (MQ=255)
                                                      tatTTGAGGCAAGAGGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGcc                                                  <  1:3167913/101‑1 (MQ=255)
                                                       atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGccc                                                 >  1:106802/1‑100 (MQ=255)
                                                        tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATGTCCCGGGGGGTGAA.TGGCGGA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCcgc                                                <  1:2774647/101‑1 (MQ=37)
                                                           gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGAc                                             <  1:1953156/101‑1 (MQ=255)
                                                           gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGAc                                             <  1:2674363/101‑1 (MQ=255)
                                                            gggaaaGATGAG...GGGG..GGTTTTCATCTTCCCGAAAATACCGGGGGGGGGGAA.GGGCGCA..GCCAGGGGGGGGCAGCCC.C.C.C.CCTTCGCGGGCGGCCGCGAcg                                            <  1:3329894/97‑1 (MQ=255)
                                                                aaGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGAcgcgcg                                        >  2:3307546/1‑101 (MQ=255)
                                                                 aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACgcgcgc                                       >  2:1297249/1‑101 (MQ=255)
                                                                  gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGcgcc                                      <  2:2847709/101‑1 (MQ=255)
                                                                   aTGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGcgccg                                     >  2:3922272/1‑101 (MQ=255)
                                                                    tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGccccac                                    >  2:3969880/1‑99 (MQ=255)
                                                                           gggg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACa                                >  1:2461999/1‑101 (MQ=255)
                                                                              g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.C.C.CGCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                             >  2:243587/1‑101 (MQ=255)
                                                                                 ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                            <  2:4536517/101‑1 (MQ=255)
                                                                                       cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                      <  2:358880/101‑1 (MQ=255)
                                                                                       cATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGccc                      >  2:2856412/1‑101 (MQ=255)
                                                                                        aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                     >  2:4121141/1‑101 (MQ=255)
                                                                                          cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                   >  1:1769054/1‑101 (MQ=255)
                                                                                          cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                   >  2:3402257/1‑101 (MQ=255)
                                                                                           ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                  <  2:505808/101‑1 (MQ=255)
                                                                                           ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCAAGAGGGGGGAAG.AG.CAA.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                  >  1:607164/1‑101 (MQ=38)
                                                                                             gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                >  2:230904/1‑101 (MQ=255)
                                                                                             gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                <  1:4658713/101‑1 (MQ=255)
                                                                                             gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                <  1:674676/101‑1 (MQ=255)
                                                                                                gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCCC.C.C.C.CCTTCGCGGGGGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt             <  1:1468801/101‑1 (MQ=38)
                                                                                                gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt             <  1:3448326/101‑1 (MQ=255)
                                                                                                 aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt            <  1:3643399/101‑1 (MQ=255)
                                                                                                 aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.C.CAC.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt            >  2:1904205/1‑101 (MQ=255)
                                                                                                   aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt          >  2:4167126/1‑101 (MQ=255)
                                                                                                   aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt          >  1:4276132/1‑101 (MQ=255)
                                                                                                    atatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTg         >  1:2056425/1‑101 (MQ=255)
                                                                                                    atatCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTGGTGCCCCTTTGTCCTTCTg         >  1:422611/1‑101 (MQ=37)
                                                                                                      atCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc       <  1:2239251/101‑1 (MQ=255)
                                                                                                      atCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc       >  2:1144011/1‑101 (MQ=255)
                                                                                                      atCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc       <  2:3122330/101‑1 (MQ=255)
                                                                                                       tCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCGTTGTCATTCTGttg      >  2:2566277/1‑99 (MQ=255)
                                                                                                        cgcGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc     <  2:4243666/99‑1 (MQ=255)
                                                                                                         ccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCa    <  1:2215507/101‑1 (MQ=255)
                                                                                                         ccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCC.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGCCATTCTGTCGCa    >  1:3894795/1‑101 (MQ=255)
                                                                                                           |                                                                                                           
CCGGGGCAAGAAGGGGGCTCT.G.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.C.C.C.CCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACC  >  NC_014034/2249259‑2249457

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG