Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,341,833 Δ1 bp 100% intergenic (‑75/+107) purS ← / ← purC phosphoribosylformylglycinamidine synthase subunit PurS/phosphoribosylaminoimidazole‑succinocarboxamide synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,341,8330C→.100.0% 790.3 179intergenic (‑75/+107)purS/purCphosphoribosylformylglycinamidine synthase subunit PurS/phosphoribosylaminoimidazole‑succinocarboxamide synthase
Reads supporting (aligned to +/- strand):  new base (104/75):  ref base (0/0):  total (104/75)

Only 100 of 206 total aligned reads displayed.

AGCATCACATGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAGCCCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCTTCGCTCCTG  >  NC_014034/2341733‑2341929
                                                                                                    |                                                                                                
 gCATCACATGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAGcc                                                                                                 <  2:3669796/101‑1 (MQ=255)
  cATCACATGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAGccc                                                                                                >  1:3358164/1‑101 (MQ=255)
  cATCACATGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGACAGCCCAAGccc                                                                                                >  1:3478428/1‑101 (MQ=255)
   aTCACATGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGTTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAGcccc                                                                                               >  1:2774778/1‑101 (MQ=255)
     cacaTGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAGccccgg                                                                                             >  1:3399814/1‑99 (MQ=255)
     cacaTGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAGccccgg                                                                                             >  2:2853558/1‑99 (MQ=255)
      acaTGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAGccccggc                                                                                            >  1:885062/1‑98 (MQ=255)
       caTGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAGccccggcc                                                                                           >  2:3824494/1‑97 (MQ=255)
       caTGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGACCTGATAGCCCAAGccccggcc                                                                                           >  1:1013611/1‑97 (MQ=255)
             cacGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGc                                                                                    <  1:4502202/101‑1 (MQ=255)
             cacGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGc                                                                                    <  2:4383695/101‑1 (MQ=255)
              acGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCa                                                                                   >  2:168945/1‑101 (MQ=255)
               cGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGcca                                                                                  >  1:1105034/1‑99 (MQ=255)
               cGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCaa                                                                                  >  1:2213330/1‑101 (MQ=255)
                gggCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAg                                                                                 <  2:3714837/101‑1 (MQ=255)
                gggCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAg                                                                                 >  2:2977514/1‑101 (MQ=255)
                gggCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAg                                                                                 >  1:3004416/1‑101 (MQ=255)
                   cTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAgcgc                                                                              >  1:2850723/1‑101 (MQ=255)
                   cTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAgcgc                                                                              >  1:3265203/1‑101 (MQ=255)
                    tttCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGcgcg                                                                             >  2:3558116/1‑101 (MQ=255)
                     ttCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCGGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGa                                                                            >  2:3604470/1‑101 (MQ=255)
                      tCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGat                                                                           <  2:3117690/101‑1 (MQ=255)
                      tCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGat                                                                           <  1:1058058/101‑1 (MQ=255)
                      tCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGGGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGat                                                                           >  2:4042728/1‑101 (MQ=255)
                       cATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGatg                                                                          >  1:1233246/1‑101 (MQ=255)
                       cATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGatg                                                                          <  2:2228816/101‑1 (MQ=255)
                        aTCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGatgg                                                                         >  1:4705316/1‑101 (MQ=255)
                        aTCCGGGAGCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGatgg                                                                         <  2:2884658/101‑1 (MQ=255)
                           cGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGatggcgc                                                                      >  2:1784054/1‑101 (MQ=255)
                            gggATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGatggcgca                                                                     >  1:944830/1‑101 (MQ=255)
                             ggATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGaaggcgcat                                                                    >  1:4328612/1‑101 (MQ=255)
                             ggATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGGGGCCGCACCCGTCCGCTTCGTCCCTAATAGTCCAAG.CCCAGGCCTCGGGCACGCGCGatagcgcat                                                                    >  2:1534199/1‑101 (MQ=255)
                              gATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGatggcgcatg                                                                   <  1:709220/101‑1 (MQ=255)
                                 ctcctcGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCCCCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGatggcgcatggcg                                                                <  1:980448/101‑1 (MQ=255)
                                    ctcGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCgg                                                             >  1:3033972/1‑101 (MQ=255)
                                     tcGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCggg                                                            >  2:2774993/1‑101 (MQ=255)
                                      cGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGgc                                                           <  1:67256/101‑1 (MQ=255)
                                      cGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGgc                                                           <  1:1877566/101‑1 (MQ=255)
                                         ttGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCa                                                        >  2:2941240/1‑101 (MQ=255)
                                          tGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAg                                                       <  2:2213206/101‑1 (MQ=255)
                                          tGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAg                                                       >  2:4639484/1‑101 (MQ=255)
                                                    gTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGc                                             >  1:3933446/1‑101 (MQ=255)
                                                    gTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGc                                             >  2:4154692/1‑101 (MQ=255)
                                                     tctcGACAGGTGGCCGCGCCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGcc                                            <  2:726433/101‑1 (MQ=255)
                                                     tctcGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGcc                                            >  1:2980291/1‑101 (MQ=255)
                                                     tctcGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGcc                                            <  1:1185313/101‑1 (MQ=255)
                                                      ctcGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCa                                           >  2:1841489/1‑101 (MQ=255)
                                                        cGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCaaa                                         <  1:1552140/101‑1 (MQ=255)
                                                         gACAGGTGGCCGCACCGGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAAc                                        <  1:3604689/101‑1 (MQ=255)
                                                          aCAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCCAACg                                       >  2:4009791/1‑101 (MQ=255)
                                                          aCAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACg                                       <  2:325567/101‑1 (MQ=255)
                                                          aCAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGACCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCCAACg                                       >  2:4108872/1‑101 (MQ=255)
                                                          aCAGGTGGCCGCACCCGTCCGGTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACg                                       >  1:2700722/1‑101 (MQ=255)
                                                           cAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGGCAAACGa                                      >  1:3289289/1‑101 (MQ=255)
                                                           cAGGCGGCCGCCCCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGa                                      <  2:4752975/101‑1 (MQ=255)
                                                            aGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGaa                                     >  2:4651834/1‑101 (MQ=255)
                                                            aGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGaa                                     >  2:3333453/1‑101 (MQ=255)
                                                             ggTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGaaa                                    <  1:3712706/101‑1 (MQ=255)
                                                             ggTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGaaa                                    <  2:1737126/101‑1 (MQ=255)
                                                              gTGGCCGCACCCGTCGGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGGGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGaaaa                                   >  1:4005496/1‑101 (MQ=255)
                                                              gTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGaagt                                   <  2:3406996/101‑3 (MQ=255)
                                                               tGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAg                                  >  2:2443284/1‑101 (MQ=255)
                                                                ggCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGaaaaag                                 >  1:151308/1‑99 (MQ=255)
                                                                  ccGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGggc                               >  2:206076/1‑101 (MQ=255)
                                                                   cGCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGggcg                              <  1:2941416/101‑1 (MQ=255)
                                                                    gCACCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGggcgg                             <  2:3478216/101‑1 (MQ=255)
                                                                      aCCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGcc                           >  2:3369749/1‑101 (MQ=255)
                                                                      aCCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGcc                           <  1:4639740/101‑1 (MQ=255)
                                                                      aCCCGTCCGTTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAAGGGGGcc                           >  1:3231565/1‑101 (MQ=255)
                                                                        ccgtccgtTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGcccc                         <  1:50712/101‑1 (MQ=255)
                                                                          gtccgtTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGGCAAACGGAAAGGGCGGCCCcgc                       >  2:754299/1‑101 (MQ=255)
                                                                          gtccgtTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCcgc                       >  2:694367/1‑101 (MQ=255)
                                                                           tccgtTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCcgcg                      <  2:2565030/101‑1 (MQ=255)
                                                                            ccgtTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCgg                     >  2:3495070/1‑101 (MQ=255)
                                                                             cgtTTCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCggg                    <  1:1841601/101‑1 (MQ=255)
                                                                               tttCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGGCCCGCggggc                  >  1:4373596/1‑99 (MQ=255)
                                                                                ttCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGAcc                 <  1:4679239/101‑1 (MQ=255)
                                                                                 tCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGAccc                >  1:4453627/1‑100 (MQ=255)
                                                                                 tCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCg                >  2:3041968/1‑101 (MQ=255)
                                                                                 tCGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCg                <  2:3004228/101‑1 (MQ=255)
                                                                                  cGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGc               >  2:1288610/1‑101 (MQ=255)
                                                                                  cGGCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGc               >  1:2072665/1‑101 (MQ=255)
                                                                                   ggCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGGCCAACGAAAAGGGCGGCCCCGCCGGGCCGcc              >  1:171820/1‑101 (MQ=255)
                                                                                   ggCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGcc              >  1:135450/1‑101 (MQ=255)
                                                                                   ggCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGcc              >  2:1395475/1‑101 (MQ=255)
                                                                                   ggCCCTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGcc              >  2:538122/1‑101 (MQ=255)
                                                                                     cccTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAAGGCGGCCCCGCGGGACCGcccc            >  1:2528040/1‑101 (MQ=255)
                                                                                      ccggATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGTGCGGCCCCGCGGGACCGCCCCt           <  2:4426957/98‑1 (MQ=255)
                                                                                      ccTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCt           <  2:1246915/101‑1 (MQ=255)
                                                                                      ccTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCt           >  2:2662097/1‑101 (MQ=255)
                                                                                      ccTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCt           >  2:3126980/1‑101 (MQ=255)
                                                                                       cTGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCtt          >  2:1609586/1‑101 (MQ=255)
                                                                                        tGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCTTc         >  1:2362756/1‑101 (MQ=255)
                                                                                        tGATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATAGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAAGGCGGCCCCGCGGGGCCGCCCCTTc         >  2:2421939/1‑101 (MQ=255)
                                                                                        tGAGAGCCCAAG.CCCGGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCTTc         <  2:3419827/101‑1 (MQ=255)
                                                                                         gATAGCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCTTCg        >  1:3836600/1‑101 (MQ=255)
                                                                                             gCCCAAG.CCCCGGCCTCGGGCAAGCGGGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCAAAACGAAAAGGGCAGCCCCGCGGGACCGCCCCTTCGCTcc    <  2:1049832/101‑1 (MQ=255)
                                                                                             gCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAGAAGGGCGGCCCCGCGGGGCCGCCCCTTCGCTcc    >  1:4106038/1‑101 (MQ=255)
                                                                                             gCCCAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCTTCGCTcc    >  2:4544984/1‑101 (MQ=255)
                                                                                              cccAAG.CCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATGGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCTTCGCTCCt   <  2:4328365/101‑1 (MQ=255)
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AGCATCACATGCACACGGGCTTTCATCCGGGATCTCCTCGCTTGGGGCTGGCGTCTCGACAGGTGGCCGCACCCGTCCGTTTCGGCCCTGATAGCCCAAGCCCCCGGCCTCGGGCAAGCGCGATGGCGCATGGCGCGGGCGCAGATTGGCGGGCCAAACGAAAAGGGCGGCCCCGCGGGACCGCCCCTTCGCTCCTG  >  NC_014034/2341733‑2341929

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG