Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,470,253 Δ1 bp 97.5% intergenic (‑186/+31) RCAP_rcc02294 ← / ← proB signal transduction histidine kinase/glutamate 5‑kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,470,2530G→.97.5% 7.8 ‑0.4 ‑0.6198intergenic (‑186/+31)RCAP_rcc02294/proBsignal transduction histidine kinase/glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  new base (79/114):  ref base (1/4):  total (80/118)
Fisher's exact test strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.28e-01

Only 100 of 230 total aligned reads displayed.

GCCTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTC  >  NC_014034/2470152‑2470349
                                                                                                     |                                                                                                
gCCTGGCTTCGGCGCGGGGACGTGGA.GACCCCCCGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAg                                                                                                  <  2:1656897/101‑1 (MQ=255)
 ccTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAg                                                                                                  <  2:3733091/101‑1 (MQ=255)
 ccTGGCTTCGGAGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGCGTTGATGAAACCAAAg                                                                                                  >  2:1017994/1‑101 (MQ=255)
  cTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgg                                                                                                 >  1:1229158/1‑101 (MQ=255)
  cTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgg                                                                                                 >  2:2227930/1‑101 (MQ=255)
  cTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgg                                                                                                 <  2:4006579/101‑1 (MQ=255)
      cTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggg                                                                                             <  2:2000211/101‑1 (MQ=255)
      cTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggg                                                                                             <  1:3254024/101‑1 (MQ=255)
         cGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggggcc                                                                                          <  2:3097885/101‑3 (MQ=255)
         cGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAgggggggcc                                                                                          <  1:4720601/101‑3 (MQ=255)
          ggCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGCcc                                                                                         >  2:4119891/1‑101 (MQ=255)
           gcgcGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCTCGGATCAACGCCGCCCGCGCGCAAACCCGGGGGTTGATGAAACCAAAGGGGGGGCcct                                                                                        >  2:2418258/1‑100 (MQ=255)
           gcgcGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGCcct                                                                                        <  2:85440/101‑2 (MQ=255)
            cgcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTg                                                                                      <  1:449202/101‑1 (MQ=255)
             gcgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCGGTATTAACGCCGCCCGCGCTCAAAACCGGGTGTTGATGAAACCAAAggggggggactgc                                                                                      <  2:4737528/101‑6 (MQ=255)
              cgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcc                                                                                    <  2:1708526/101‑1 (MQ=255)
              cgGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcc                                                                                    <  2:1537416/101‑1 (MQ=255)
                gggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccc                                                                                  <  2:1428790/101‑1 (MQ=255)
                gggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccc                                                                                  >  1:1330768/1‑101 (MQ=255)
                 ggACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGccccc                                                                                 <  2:3809229/101‑1 (MQ=255)
                  gACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGcccccc                                                                                <  2:4008488/101‑1 (MQ=255)
                   aCGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCct                                                                               <  1:725052/101‑1 (MQ=255)
                     gTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctct                                                                             >  1:851043/1‑101 (MQ=255)
                     gTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCctct                                                                             <  1:266276/101‑1 (MQ=255)
                       tgatgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGc                                                                           <  1:4628654/101‑1 (MQ=255)
                       tgatgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGc                                                                           <  1:2998926/101‑1 (MQ=255)
                       tgatgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGc                                                                           >  1:3077919/1‑101 (MQ=255)
                         atgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGccc                                                                         >  1:3038199/1‑101 (MQ=255)
                          tgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGcccc                                                                        <  2:2255967/101‑1 (MQ=255)
                          tgaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGcccc                                                                        >  1:1737028/1‑101 (MQ=255)
                           gaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCg                                                                       >  1:2228782/1‑101 (MQ=255)
                           gaCGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCg                                                                       <  1:1604199/101‑1 (MQ=255)
                             cgcgcgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGc                                                                     <  1:2111677/101‑1 (MQ=255)
                                gcgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAACCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTc                                                                  <  2:213082/101‑1 (MQ=255)
                                gcgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTc                                                                  <  2:3422894/101‑1 (MQ=255)
                                 cgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                 <  2:4580309/101‑1 (MQ=255)
                                 cgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                 >  2:3869647/1‑101 (MQ=255)
                                 cgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                 <  1:2410318/101‑1 (MQ=255)
                                 cgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                 <  1:3949945/101‑1 (MQ=255)
                                 cgGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCa                                                                 <  2:2560705/101‑1 (MQ=255)
                                     cgcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCg                                                             <  2:3838117/101‑1 (MQ=255)
                                      gcCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGACCTCAGCCGa                                                            >  2:52554/1‑101 (MQ=255)
                                        cAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATc                                                          <  2:4546799/101‑1 (MQ=255)
                                        cAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATc                                                          <  1:48415/101‑1 (MQ=255)
                                         aaaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCa                                                         >  2:3971090/1‑101 (MQ=255)
                                          aaGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCAc                                                        <  1:4423346/101‑1 (MQ=255)
                                            ggAACGCGCCCGGATCAACGCTGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCa                                                      >  2:191848/1‑101 (MQ=255)
                                            ggAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCa                                                      <  1:3418954/101‑1 (MQ=255)
                                              aaCGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATg                                                    <  1:2463687/101‑1 (MQ=255)
                                              aaCGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATg                                                    >  1:2874506/1‑101 (MQ=255)
                                               aCGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgt                                                   <  2:4330344/101‑1 (MQ=255)
                                                cgcgCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgtc                                                  <  2:1330676/101‑1 (MQ=255)
                                                 gcgcCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgtcg                                                 <  1:3802712/101‑1 (MQ=255)
                                                   ccccGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGAGGAAACC.AAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATgtcgtc                                               <  1:1667411/100‑1 (MQ=255)
                                                    cccGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGtcgtcg                                              <  1:1282156/101‑1 (MQ=255)
                                                      cGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTcgcg                                            >  2:2807987/1‑101 (MQ=255)
                                                      cGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTcgcg                                            >  1:2354684/1‑101 (MQ=255)
                                                        gATCAACGCCGCCCGCGCGCAAACCCCGGTGTTGATGAAACCAAA.TGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGAt                                          <  1:3989362/101‑1 (MQ=255)
                                                           cAACGCCGCCCGGGCGCAACCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGCCGTCGCGATGca                                       <  1:2570416/101‑1 (MQ=255)
                                                            aaCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcac                                      >  2:1743302/1‑101 (MQ=255)
                                                             aCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcaca                                     <  1:2559487/101‑1 (MQ=255)
                                                             aCGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGcaca                                     <  1:129017/101‑1 (MQ=255)
                                                              cgccgcCCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACaa                                    <  2:3391987/101‑1 (MQ=255)
                                                               gccgccCGCGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCACCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAg                                   <  2:4433099/101‑1 (MQ=255)
                                                                ccgcccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgc                                  <  1:3339412/101‑1 (MQ=255)
                                                                ccgcccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgc                                  >  2:3290560/1‑101 (MQ=255)
                                                                ccgcccgcGCGCAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgc                                  >  1:4331992/1‑101 (MQ=255)
                                                                     cgcgcgCAAACCCGGGTGTTGAGGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAgcgcggc                             <  1:2793662/101‑1 (MQ=255)
                                                                         cgcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGcgcggcgcgg                         >  2:920694/1‑101 (MQ=255)
                                                                          gcAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCgcggcgcggc                        >  1:631225/1‑101 (MQ=255)
                                                                           cAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGcc                       >  2:2754989/1‑101 (MQ=255)
                                                                           cAAACCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGcc                       <  1:3971332/101‑1 (MQ=255)
                                                                            aaaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGccc                      <  2:231925/101‑1 (MQ=255)
                                                                             aaCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCg                     >  2:237073/1‑101 (MQ=255)
                                                                              aCCCGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCgg                    <  1:3530935/101‑1 (MQ=255)
                                                                                ccGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGAt                  <  1:1811386/101‑1 (MQ=255)
                                                                                ccGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGAt                  <  1:4390110/101‑1 (MQ=255)
                                                                                 cGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATa                 >  2:811478/1‑101 (MQ=255)
                                                                                 cGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATa                 <  1:1841141/101‑1 (MQ=255)
                                                                                 cGGGTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATa                 <  1:3168559/101‑1 (MQ=255)
                                                                                  gggTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAg                >  1:3774146/1‑101 (MQ=255)
                                                                                  gggTGGTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAg                <  1:3883446/101‑1 (MQ=255)
                                                                                   ggTGTTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGc               <  1:4025433/101‑1 (MQ=255)
                                                                                     tgtTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGccc             <  1:108008/101‑1 (MQ=255)
                                                                                     tgtTGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGccc             >  1:1858434/1‑101 (MQ=255)
                                                                                       ttGATGAAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcag           <  2:2244457/101‑1 (MQ=255)
                                                                                       ccgatctaaCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcag           <  2:3492834/94‑1 (MQ=255)
                                                                                        tgatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagc          >  2:3565981/1‑101 (MQ=255)
                                                                                        tgatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagc          >  1:2822308/1‑101 (MQ=255)
                                                                                        tgatgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagc          >  2:1057921/1‑101 (MQ=255)
                                                                                          atgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCgagcag        <  1:1148213/101‑1 (MQ=255)
                                                                                          atgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagcag        <  1:381691/101‑1 (MQ=255)
                                                                                          atgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagcag        <  2:644782/101‑1 (MQ=255)
                                                                                          atgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagaag        <  1:2715760/101‑1 (MQ=255)
                                                                                           tgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCagcagc       >  1:1402316/1‑101 (MQ=255)
                                                                                           tgaAACCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCagcagc       >  2:1298549/1‑101 (MQ=255)
                                                                                             aaaCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAgcgc     >  1:1066968/1‑101 (MQ=255)
                                                                                              aaCCAAA.GGGGGGGCCCTGCCGCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCcagagatcgg    >  1:3493046/1‑94 (MQ=255)
                                                                                              aaCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGcc    >  1:2005466/1‑101 (MQ=255)
                                                                                               aCCAAA.GGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCt   <  1:4555380/101‑1 (MQ=255)
                                                                                                     |                                                                                                
GCCTGGCTTCGGCGCGGGGACGTTGATGACGCGCGGGCGCCAAAGGAACGCGCCCGGATCAACGCCGCCCGCGCGCAAACCCGGGTGTTGATGAAACCAAAGGGGGGGGCCCTGCCCCCCTCTGCCCCGGCCTCAGCCGATCACCATGTCGTCGCGATGCACAAGCGCGGCGCGGCCCGGATAGCCCAGCAGCGCCTC  >  NC_014034/2470152‑2470349

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG