Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,493,971 Δ1 bp 4.2% intergenic (+19/‑223) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,493,9710G→. 4.2% 14.1 ‑0.9 ‑0.1166intergenic (+19/‑223)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/6):  ref base (54/105):  total (55/111)
Fisher's exact test strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.99e-01

Only 100 of 184 total aligned reads displayed.

TGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAA.GGGG.GGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAGCAGA  >  NC_014034/2493876‑2494067
                                                                                                  |                                                                                                
tGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGGGCTGCCCCCCGTCCg                                                                                              >  1:3511809/1‑101 (MQ=255)
 gATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGc                                                                                             <  2:2578019/101‑1 (MQ=39)
  ttgccggaccTGCCCCCCCGCGGCCCGCGTCTGGCCCAGGGCCGCGGCGGGCGCGCGGCCCGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCa                                                                                            <  2:4727931/92‑1 (MQ=21)
  aTGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCa                                                                                            <  1:4753797/101‑1 (MQ=39)
      ccGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCCAGCg                                                                                        >  1:577410/1‑101 (MQ=38)
       cGAGCTGCCCGCCCGCGGGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCgg                                                                                       <  2:281402/101‑1 (MQ=37)
       cGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCgg                                                                                       <  1:2356138/101‑1 (MQ=38)
        ggccTGCCCACCCGCGCGCCGCGTCTGGGCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGa                                                                                      <  2:3511597/98‑1 (MQ=37)
        gAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTGCGCAAGCGGa                                                                                      >  2:1970463/1‑101 (MQ=38)
        gAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGa                                                                                      <  2:1980612/101‑1 (MQ=38)
        gAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGa                                                                                      >  2:2875425/1‑101 (MQ=38)
            tGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGGCCGCAAGCGGACTcc                                                                                  >  2:401983/1‑101 (MQ=37)
              cccACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTcccc                                                                                <  1:3756723/101‑1 (MQ=37)
               ccACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTccccc                                                                               <  2:4188737/101‑1 (MQ=37)
                 ccccGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCg                                                                             <  1:3305459/100‑1 (MQ=25)
                  cccGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCgg                                                                            >  1:2067404/1‑101 (MQ=25)
                  cccGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCgg                                                                            <  1:1585203/101‑1 (MQ=25)
                   ccGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCggg                                                                           <  2:4380728/101‑1 (MQ=25)
                     gcgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.GGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGat                                                                         <  1:3776234/101‑1 (MQ=25)
                     gcgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGat                                                                         <  2:843134/101‑1 (MQ=25)
                     gcgcccCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGat                                                                         <  1:317568/101‑1 (MQ=25)
                      cgcgcCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGata                                                                        <  2:3698700/101‑1 (MQ=25)
                        cgccgcGTCTGGCCCAGCGCCGCGGCGGCCGCGCGGCACGACAGGCCG.AGTAA.GGGGTGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATAtt                                                                      <  2:1845542/101‑1 (MQ=25)
                         gccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATAttt                                                                     <  1:1204872/101‑1 (MQ=25)
                          ccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTg                                                                    <  1:1054021/101‑1 (MQ=25)
                          ccgcGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTg                                                                    <  1:1375086/101‑1 (MQ=25)
                           cgcgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgc                                                                   <  1:220489/101‑1 (MQ=25)
                           cgcgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGAGATTTgc                                                                   >  1:4386250/1‑101 (MQ=25)
                             cgTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcag                                                                 <  2:4459837/101‑1 (MQ=25)
                              gTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTgcagc                                                                <  1:3005772/101‑1 (MQ=25)
                                cTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGGGCTGCCCCCCCTCCGCAAGCGGGCTCCCCCCCGGGGATTTTCCGCaa                                                              >  2:2707512/1‑101 (MQ=255)
                                  ggCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCCAGa                                                            >  2:480737/1‑101 (MQ=21)
                                  ggCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGa                                                            <  1:3984174/101‑1 (MQ=21)
                                   gCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGAt                                                           <  1:3608562/101‑1 (MQ=21)
                                    cccAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATATTTGCAGGAAGacg                                                          >  1:81849/1‑99 (MQ=21)
                                      cAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                                        <  2:2122270/101‑1 (MQ=21)
                                      cAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaa                                                        <  1:1135872/101‑1 (MQ=21)
                                       atggccggGGCGGGCGCGCGGCCCGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                       <  2:1497035/98‑1 (MQ=17)
                                       aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                       <  1:1287612/101‑1 (MQ=21)
                                       aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                       <  2:1620984/101‑1 (MQ=21)
                                       aGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGaaa                                                       <  2:1427296/101‑1 (MQ=21)
                                        gcgcGGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                      <  2:4218986/101‑1 (MQ=18)
                                        gcgcCGGGGCGGGCGCGCGGCAGGCCAGACCGAAGAAG.GGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAc                                                      <  1:2938237/101‑1 (MQ=14)
                                            cgcgGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGGGCTGCCCCCCGTCCGCAAGCGGGCTCCCCCCGGGGTCTTTGGCGCAAGATGAAACCggg                                                  >  2:2920256/1‑100 (MQ=18)
                                            cgcgGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                  <  1:1867603/101‑1 (MQ=21)
                                            cgcgGCGGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGa                                                  <  2:2829653/101‑1 (MQ=21)
                                             gcggcgGGCGCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAt                                                 <  2:2269298/101‑1 (MQ=21)
                                                 cgggcgCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                             <  2:902414/101‑1 (MQ=21)
                                                  gggcgCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                            <  1:4035747/101‑1 (MQ=21)
                                                  gggcgCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCCGGATCTTTGCAGCAAGATGAAAACCGAGGCCtt                                            >  1:1629492/1‑101 (MQ=17)
                                                   ggcgCGCGGCACGACAGGCCG.AGTAAGGGGG.GGGCCCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCttt                                           <  2:4267536/101‑1 (MQ=21)
                                                    gcgcgcGGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                          <  1:4238216/101‑1 (MQ=21)
                                                      gcgcgGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                        >  2:4475525/1‑101 (MQ=21)
                                                      gcgcgGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTAAt                                        >  1:3671805/1‑101 (MQ=18)
                                                       cgcgGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                       >  2:3321987/1‑101 (MQ=21)
                                                         cgGCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                     >  2:2651574/1‑101 (MQ=21)
                                                          ggCACGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                    <  2:3990192/101‑1 (MQ=21)
                                                             aCGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGGAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                 >  2:2360973/1‑101 (MQ=21)
                                                              cGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                >  1:944194/1‑101 (MQ=21)
                                                              cGACAGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                <  2:3405855/101‑1 (MQ=21)
                                                                  aGGCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                            >  2:1270726/1‑101 (MQ=21)
                                                                   ggCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                           <  1:2629970/101‑1 (MQ=21)
                                                                   ggCCG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                           <  2:1003329/101‑1 (MQ=21)
                                                                      cG.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATcc                        >  1:2765184/1‑101 (MQ=18)
                                                                       g.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                       <  1:2875601/101‑1 (MQ=18)
                                                                       g.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                       >  1:260317/1‑101 (MQ=18)
                                                                       g.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                       >  2:3439104/1‑101 (MQ=18)
                                                                       g.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                       <  1:1960456/101‑1 (MQ=18)
                                                                       g.AGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                       >  2:3097438/1‑101 (MQ=18)
                                                                        gaggaaggggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                       <  1:305139/94‑1 (MQ=18)
                                                                         aGTAAGGGGG.GGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAGTATCCCg                      >  2:4149881/1‑101 (MQ=18)
                                                                          gtaaggggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                     <  2:1688279/96‑1 (MQ=255)
                                                                           taaggggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                    >  2:4753295/5‑101 (MQ=255)
                                                                           taaggggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                    >  2:1381320/5‑101 (MQ=255)
                                                                            aaggggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                   >  2:3228300/4‑101 (MQ=255)
                                                                            aaggggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                   >  2:2740666/4‑101 (MQ=255)
                                                                             aggggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                  <  2:2608289/99‑1 (MQ=255)
                                                                             aggggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                  >  1:1229022/3‑101 (MQ=255)
                                                                              ggggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                 >  1:1430865/2‑101 (MQ=255)
                                                                              ggggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGACGCCTTTCATCTTGCCCGAAATATCCCgggggg                 >  1:4450895/2‑101 (MQ=255)
                                                                              ggggg.gcgCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                 <  1:783717/100‑1 (MQ=255)
                                                                               gggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                <  1:2651728/101‑1 (MQ=255)
                                                                               gggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                >  2:4551779/1‑101 (MQ=255)
                                                                                ggg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg               >  2:3922708/1‑101 (MQ=255)
                                                                                 gg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa              >  1:4641123/1‑101 (MQ=255)
                                                                                 gg.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa              <  1:1970580/101‑1 (MQ=255)
                                                                                  g.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa             <  2:3812296/101‑1 (MQ=255)
                                                                                  g.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGaa             >  1:4360479/1‑101 (MQ=255)
                                                                                  g.gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGaa             >  1:4682235/1‑101 (MQ=255)
                                                                                    gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAAt            <  1:1520811/101‑1 (MQ=255)
                                                                                     ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATg           <  2:3759132/101‑1 (MQ=255)
                                                                                         cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGa       >  2:4291802/1‑101 (MQ=255)
                                                                                         cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGa       <  2:940000/101‑1 (MQ=255)
                                                                                          tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGaa      >  2:98397/1‑100 (MQ=255)
                                                                                            cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGaagcc   <  1:4605223/101‑5 (MQ=255)
                                                                                            cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGaagcc   <  1:1381414/101‑5 (MQ=255)
                                                                                            cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGATCAg   <  1:2790042/101‑1 (MQ=255)
                                                                                            cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGaagc    <  1:3577036/101‑4 (MQ=255)
                                                                                            cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGaagc    <  1:4551889/101‑4 (MQ=255)
                                                                                             ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGaagcca  <  2:2546496/101‑6 (MQ=255)
                                                                                                  |                                                                                                
TGATGGCCGAGCTGCCCACCCGCGCGCCGCGTCTGGCCCAGCGCCGCGGCGGGCGCGCGGCACGACAGGCCG.AGTAA.GGGG.GGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAGCAGA  >  NC_014034/2493876‑2494067

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG