Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,022 Δ1 bp 6.4% intergenic (+70/‑172) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0220C→. 6.4% 27.6 ‑0.6 ‑0.6173intergenic (+70/‑172)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (80/81):  total (88/85)
Fisher's exact test strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.34e-01

Only 100 of 186 total aligned reads displayed.

GGCGGGCGCGCGGCACGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A..G.CAGAAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493923‑2494121
                                                                                                     |                                                                                                            
     gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                         <  2:2192603/101‑1 (MQ=21)
     gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                         <  1:1545937/101‑1 (MQ=21)
     gcgcgcGGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                         <  1:4238216/101‑1 (MQ=21)
      cgcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                        <  2:887464/101‑1 (MQ=21)
       gcgcgGCACGGCGGGCAGAGTGAG.GGGGGGGCGCTGCCGCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAACCCGGATGCCTTTCAt                                                                                                       <  2:4382182/101‑1 (MQ=17)
       gcgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCAt                                                                                                       >  2:4475525/1‑101 (MQ=21)
        cgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                      <  1:1456007/101‑1 (MQ=21)
        cgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                      >  2:3321987/1‑101 (MQ=21)
        cgcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAGGCCTTTCATc                                                                                                      >  1:3655630/1‑101 (MQ=18)
         gcgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                     <  2:1119874/101‑1 (MQ=21)
          cgGCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                                    >  2:2651574/1‑101 (MQ=21)
           ggCACGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                   <  2:4007606/101‑1 (MQ=21)
              aCGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGGAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                                >  2:2360973/1‑101 (MQ=21)
               cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCTGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                               <  2:4202202/101‑1 (MQ=21)
               cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGCAATTTGCAGCAAGATGAAACCGGATGACTTTCATCTTGCCCg                                                                                               >  2:2847826/1‑101 (MQ=18)
               cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                               >  1:2437528/1‑101 (MQ=21)
               cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                               >  1:944194/1‑101 (MQ=21)
               cGACAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                               <  2:3405855/101‑1 (MQ=21)
                gACAGGCCGTGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGa                                                                                              <  2:45622/101‑1 (MQ=18)
                  cAGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGaaa                                                                                            >  2:2623504/1‑101 (MQ=21)
                   aGGCCGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAat                                                                                           >  1:2901878/1‑101 (MQ=21)
                      ccGAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                        <  1:4583406/101‑1 (MQ=21)
                        gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                      >  1:260317/1‑101 (MQ=18)
                        gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                      >  2:3097438/1‑101 (MQ=18)
                        gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                      >  2:3439104/1‑101 (MQ=18)
                        gAGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                      <  1:1960456/101‑1 (MQ=18)
                         gaggaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATccc                                                                                      <  1:305139/94‑1 (MQ=18)
                         aGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAGTATCCCg                                                                                     >  2:4149881/1‑101 (MQ=18)
                         aGT.AAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                     <  2:911869/101‑1 (MQ=18)
                            taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                   >  2:4753295/5‑101 (MQ=255)
                            taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                   >  2:1381320/5‑101 (MQ=255)
                             aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                  >  2:3737533/4‑101 (MQ=255)
                             aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                  >  1:347592/4‑101 (MQ=255)
                              aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                 >  1:4201577/3‑101 (MQ=255)
                              aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                 <  2:2608289/99‑1 (MQ=255)
                              aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                 >  1:1229022/3‑101 (MQ=255)
                               ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                                >  1:1430865/2‑101 (MQ=255)
                               ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGACGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                                >  1:4450895/2‑101 (MQ=255)
                               ggggggcgCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                                <  1:783717/100‑1 (MQ=255)
                                gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                               >  1:3835710/1‑100 (MQ=255)
                                gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                               <  1:2651728/101‑1 (MQ=255)
                                gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGCGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGt                                                                               >  2:4551779/1‑101 (MQ=255)
                                 ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                              >  1:1625525/1‑101 (MQ=255)
                                 ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                              >  2:3922708/1‑101 (MQ=255)
                                   ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGaa                                                                            >  1:4360479/1‑101 (MQ=255)
                                     ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                         <  2:1976251/101‑1 (MQ=255)
                                     ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                         <  2:3759132/101‑1 (MQ=255)
                                         cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                  >  2:4291802/1‑101 (MQ=255)
                                         cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                  <  1:3999310/101‑1 (MQ=255)
                                            cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                              <  1:480788/101‑5 (MQ=255)
                                            cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                              >  2:3871363/1‑97 (MQ=255)
                                            cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                              <  2:4121073/101‑5 (MQ=255)
                                            cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A..T.CAg                                                           <  1:2790042/101‑1 (MQ=255)
                                            cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                               <  1:4551889/101‑4 (MQ=255)
                                            cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                               <  1:3577036/101‑4 (MQ=255)
                                             ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                             <  2:2546496/101‑6 (MQ=255)
                                                ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                           <  1:2848002/98‑8 (MQ=255)
                                                ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                           >  2:617701/4‑94 (MQ=255)
                                                      gCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AAGG.CAGAGggggg                                                    >  1:1601862/1‑101 (MQ=18)
                                                        aaGCGGGCTCCCGCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGCa                                                 <  1:2260694/101‑1 (MQ=18)
                                                          gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGCagc                                               >  1:2176995/1‑99 (MQ=18)
                                                          gCGGACTCCCCCCGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGCaga                                               >  1:4734439/1‑99 (MQ=18)
                                                            ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAC.TGGC..GAA..G.CCAGAGGGGGGCagcgc                                             >  1:4638215/1‑97 (MQ=17)
                                                            ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGCagcgc                                             >  1:4676075/1‑97 (MQ=18)
                                                            ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGCagcgc                                             >  2:172563/1‑97 (MQ=18)
                                                            ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGACCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGCagcgc                                             >  1:2769520/1‑97 (MQ=17)
                                                             gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCG.c.c                                         >  1:2725543/1‑101 (MQ=17)
                                                               cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCAG.AGGGGGGC.AGCG.c.ccc                                       <  2:2954777/101‑1 (MQ=17)
                                                               cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..GCCAG.AGGGGGGC.AGCG.c.ccc                                       <  1:402027/101‑1 (MQ=17)
                                                                tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCG.c.cccc                                      >  2:128706/1‑101 (MQ=17)
                                                                tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCG.c.cccc                                      >  1:806307/1‑101 (MQ=17)
                                                                  cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCG.c.ccccct                                    <  1:4471390/101‑3 (MQ=17)
                                                                  cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCG.c.ccccct                                    <  2:848858/101‑3 (MQ=17)
                                                                       gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGcccc                                <  2:191057/101‑1 (MQ=17)
                                                                        ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A..G.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcg                               >  1:659612/1‑101 (MQ=2)
                                                                        ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcg                               <  1:4417932/101‑1 (MQ=17)
                                                                          atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A..G.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcgcc                             >  1:2418385/1‑99 (MQ=2)
                                                                           tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggcg                            >  1:1636751/1‑101 (MQ=18)
                                                                            atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggcgg                           >  2:1445592/1‑101 (MQ=18)
                                                                            atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCG.CACCCCTGCCCcggcgg                           >  2:2029738/1‑101 (MQ=18)
                                                                             tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggcggc                          >  2:977702/1‑100 (MQ=17)
                                                                               tGCAGCAAGATGAAACCGGATGCCTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTcc                        <  1:3190927/101‑1 (MQ=17)
                                                                                gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                       >  1:4463377/1‑101 (MQ=17)
                                                                                 cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGt                      <  1:4346247/101‑1 (MQ=17)
                                                                                  agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTg                     <  1:998655/101‑1 (MQ=17)
                                                                                    caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGG.AAGCGCC.CCCCTGCCCCGGCGGTCCGTggc                   >  1:345412/1‑101 (MQ=18)
                                                                                     aaGATCAAACCGGATGCCTTTCATCTTGCCAGAAATCGCCAGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcg                  <  1:836859/101‑1 (MQ=9)
                                                                                      aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcgg                 >  2:1161814/1‑101 (MQ=18)
                                                                                       gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcggc                >  2:4232837/1‑101 (MQ=17)
                                                                                        aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGgcggcg               >  2:1882578/1‑101 (MQ=18)
                                                                                          gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGcc             >  1:2626861/1‑101 (MQ=17)
                                                                                           aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGAGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCt            >  1:2831091/1‑101 (MQ=18)
                                                                                            aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt           <  1:4650818/101‑1 (MQ=17)
                                                                                             aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg          <  1:3335693/101‑1 (MQ=17)
                                                                                               cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        <  2:1041092/101‑1 (MQ=17)
                                                                                                ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCcg       >  1:1223598/1‑101 (MQ=17)
                                                                                                 gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg      <  1:2311185/101‑1 (MQ=17)
                                                                                                  aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg     <  1:1172984/101‑1 (MQ=17)
                                                                                                   tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc    <  1:4037518/101‑1 (MQ=17)
                                                                                                     ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA..G.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg  <  1:1254947/101‑1 (MQ=17)
                                                                                                     |                                                                                                            
GGCGGGCGCGCGGCACGACAGGCCGAGT.AA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A..G.CAGAAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493923‑2494121

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG