Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,047 Δ1 bp 6.7% intergenic (+95/‑147) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0470G→. 6.7% 27.3 ‑0.6 ‑0.1164intergenic (+95/‑147)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (78/75):  total (85/79)
Fisher's exact test strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.40e-01

Only 100 of 187 total aligned reads displayed.

AGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCA.AGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146
                                                                                                    |                                                                                                            
aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAGTATCCCg                                                                                                              >  2:4149881/1‑101 (MQ=18)
aGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCg                                                                                                              <  2:911869/101‑1 (MQ=18)
 gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                             <  2:1688279/96‑1 (MQ=255)
  taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                            >  2:1381320/5‑101 (MQ=255)
  taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                            >  2:4753295/5‑101 (MQ=255)
   aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                           >  2:2740666/4‑101 (MQ=255)
   aaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggg                                                                                                           >  2:3228300/4‑101 (MQ=255)
    aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                                          <  1:4354082/99‑1 (MQ=255)
    aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                                          <  2:2608289/99‑1 (MQ=255)
     ggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGACGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                                                         >  1:4450895/2‑101 (MQ=255)
     ggggggcgCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgggggg                                                                                                         <  1:783717/100‑1 (MQ=255)
      gggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggggg                                                                                                        >  1:3835710/1‑100 (MQ=255)
       ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                                                       >  1:1625525/1‑101 (MQ=255)
        gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAAACGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                      >  1:191082/1‑101 (MQ=255)
         ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                                                     <  2:3812296/101‑1 (MQ=255)
         ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGaa                                                                                                     >  1:4682235/1‑101 (MQ=255)
          gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                   >  2:788864/1‑101 (MQ=255)
           ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                  <  2:1976251/101‑1 (MQ=255)
               cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                           >  2:4291802/1‑101 (MQ=255)
               cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                           <  1:3999310/101‑1 (MQ=255)
                tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                          >  2:98397/1‑100 (MQ=255)
                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                       <  1:480788/101‑5 (MQ=255)
                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                       <  1:1381414/101‑5 (MQ=255)
                  cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AT.CAg                                                                                      <  1:2790042/101‑1 (MQ=255)
                  cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                        <  1:4551889/101‑4 (MQ=255)
                       cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                   <  2:2067283/99‑9 (MQ=255)
                         tCCGCAAGCGGACTCCCCCCGGGAGATTTGCAGCAAGATGAAACCGGATACCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagaggg                                                                                 >  1:43063/1‑91 (MQ=18)
                              aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCa                                                                          <  1:1431276/101‑1 (MQ=18)
                                gCGGACTCCCCCCGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCaga                                                                        >  1:4734439/1‑99 (MQ=18)
                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAC.TGGC..GAAG.CCA..GAGGGGGGCagcgc                                                                      >  1:4638215/1‑97 (MQ=17)
                                  ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCagcgc                                                                      >  2:172563/1‑97 (MQ=18)
                                    aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCG.ccc                                                                 >  1:283443/1‑101 (MQ=18)
                                    aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCG.ccc                                                                 >  2:2414556/1‑101 (MQ=18)
                                    aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCG.ccc                                                                 >  2:3025943/1‑101 (MQ=18)
                                     cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG...AGGGGGGC..AGCG.cccc                                                                <  1:402027/101‑1 (MQ=17)
                                      tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCG.ccccc                                                               >  1:806307/1‑101 (MQ=17)
                                      tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCG.ccccc                                                               >  1:1565968/1‑101 (MQ=18)
                                        cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCG.cccccct                                                             <  1:4471390/101‑3 (MQ=17)
                                           ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCG.cccccctgcc                                                          <  2:4018385/101‑6 (MQ=17)
                                            cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..TAGGGGGGC..AGCGCCCCCCTGccc                                                          >  1:2925682/1‑101 (MQ=18)
                                            cGGAATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGccc                                                          <  1:2203745/101‑1 (MQ=17)
                                             gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGC..C.AA.AGGCCAAGAGGGGGC.AGCG.CCCCCTGcccc                                                         <  1:4206111/101‑1 (MQ=0)
                                             gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGcccc                                                         >  1:3588006/1‑101 (MQ=17)
                                              ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGC.AGCG.CCCCCTGCCCcg                                                        >  1:659612/1‑101 (MQ=2)
                                              ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGC.AGCG.CCCCCTGCCCcg                                                        >  2:3750535/1‑101 (MQ=2)
                                               gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGC.AGCG.CCCCCTGCCCcgc                                                       >  1:4404457/1‑100 (MQ=2)
                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGC.AGCG.CCCCCTGCCCcgcc                                                      >  1:2418385/1‑99 (MQ=2)
                                                atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGC.AGCG.CCCCCTGCCCcgcc                                                      <  2:3882519/101‑3 (MQ=2)
                                                 tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGC.AGCG.CCCCCTGCCCcgccg                                                     >  2:1139753/1‑101 (MQ=2)
                                                 tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCcggcg                                                     >  1:1636751/1‑101 (MQ=18)
                                                   tttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCC..AAGAGGGGGC.AGCG.CCCCCTGCCCcgccgga                                                   >  2:2878051/1‑100 (MQ=2)
                                                     tGCAGCAAGATGAAACCGGATGCCTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTcc                                                 <  1:3190927/101‑1 (MQ=17)
                                                        agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTg                                              <  1:998655/101‑1 (MQ=17)
                                                          caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGG.A.AGCGCCCCCCTGCCCCGGCGGTCCGTggc                                            >  1:345412/1‑101 (MQ=18)
                                                           aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGCCCGTggcg                                           >  1:34950/1‑101 (MQ=17)
                                                           aaGATCAAACCGGATGCCTTTCATCTTGCCAGAAATCGCCAGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                           <  1:836859/101‑1 (MQ=9)
                                                             gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTggcggc                                         >  2:4232837/1‑101 (MQ=17)
                                                              aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGgcggcg                                        >  2:1882578/1‑101 (MQ=18)
                                                                gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGG.A.AGCGCCCCCCTGCCCCGGCGGTCCGGGGCGGGGcc                                      >  2:3683987/1‑101 (MQ=17)
                                                                 aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGGA.TGGC..GAAG.CCA..GAGGGGGGCA.GGGC.CCCCCTGCCCCCGCGGTCAGAGGCGGCGccg                                     >  1:4463056/1‑100 (MQ=12)
                                                                  aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                    <  1:4650818/101‑1 (MQ=17)
                                                                   aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                   >  1:2685816/1‑101 (MQ=18)
                                                                   aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                   <  1:3335693/101‑1 (MQ=17)
                                                                    ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                  <  1:2623656/101‑1 (MQ=17)
                                                                     cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                 <  2:4640867/101‑1 (MQ=17)
                                                                      ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCcg                                >  1:1223598/1‑101 (MQ=17)
                                                                       gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                               <  1:2311185/101‑1 (MQ=17)
                                                                        aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGCAGAGC..CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGatcgg                              >  2:857681/1‑97 (MQ=17)
                                                                        aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                              <  2:1430771/101‑1 (MQ=17)
                                                                        aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                              <  1:1172984/101‑1 (MQ=17)
                                                                           ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                           <  1:1254947/101‑1 (MQ=17)
                                                                            cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                          >  2:84451/1‑101 (MQ=17)
                                                                            cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                          >  1:3228256/1‑101 (MQ=18)
                                                                            cTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCA..GAGGGGGGCA.GCAC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGGCGTGAcgggcggg                          >  2:4502618/1‑101 (MQ=17)
                                                                             tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTTCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                         >  1:2587620/1‑101 (MQ=17)
                                                                             tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                         <  2:944117/101‑1 (MQ=17)
                                                                             tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                         <  1:4605986/101‑1 (MQ=17)
                                                                               tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                       >  2:3581/1‑100 (MQ=18)
                                                                               tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                       <  2:2564012/101‑1 (MQ=18)
                                                                               tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGAGGCGGCGCCTTGACGGGCGGGGgc                       >  2:2012970/1‑101 (MQ=17)
                                                                                 aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCGCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggggg                     >  1:4009401/1‑98 (MQ=17)
                                                                                  tCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                    <  2:2176871/101‑1 (MQ=18)
                                                                                  tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                    >  2:4376916/1‑101 (MQ=18)
                                                                                   cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                   >  2:1633663/1‑101 (MQ=18)
                                                                                     tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCACGAAGGGCCGGGGCGCgggg                 >  1:835590/1‑100 (MQ=9)
                                                                                      gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                >  2:4636090/1‑101 (MQ=18)
                                                                                        ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCTTGACGGGCGGGGGCGCGGGCAtt              >  1:2628535/1‑101 (MQ=18)
                                                                                        ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt              <  2:2304365/101‑1 (MQ=18)
                                                                                        ccGAAATACCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt              <  1:2122/101‑1 (MQ=18)
                                                                                         cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa             <  2:577359/101‑1 (MQ=18)
                                                                                          gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa            <  1:56142/101‑1 (MQ=18)
                                                                                          gAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGCCCTTCACGGGCGGGGGGGCGGGCATTaa            >  2:1959993/1‑101 (MQ=12)
                                                                                           aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc           <  1:459589/101‑1 (MQ=18)
                                                                                            aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc          <  1:2740830/101‑1 (MQ=21)
                                                                                            aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc          >  1:1127494/1‑101 (MQ=21)
                                                                                             atatCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg         <  2:4398025/101‑1 (MQ=21)
                                                                                              taccccGGGGGGTGAA.TGGC..G.AA.GCC..AGAGGGGGCC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa        <  2:1329142/98‑1 (MQ=21)
                                                                                               atCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa       <  1:2278845/101‑1 (MQ=21)
                                                                                               atCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa       >  2:1228239/1‑101 (MQ=21)
                                                                                                tCCCGGGGGGTGAA.TGGC..GAAG.CCA..GAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa      <  1:4150127/101‑1 (MQ=21)
                                                                                                    |                                                                                                            
AGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAG..AAGGGGGGCA.AGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG